Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_730 and Pf6N2E2_731 are separated by 2 nucleotides Pf6N2E2_731 and Pf6N2E2_732 overlap by 8 nucleotides Pf6N2E2_732 and Pf6N2E2_733 are separated by 10 nucleotides
Pf6N2E2_730 - Respiratory nitrate reductase beta chain (EC 1.7.99.4), at 838,011 to 839,549
_730
Pf6N2E2_731 - Respiratory nitrate reductase delta chain (EC 1.7.99.4), at 839,552 to 840,307
_731
Pf6N2E2_732 - Respiratory nitrate reductase gamma chain (EC 1.7.99.4), at 840,300 to 840,980
_732
Pf6N2E2_733 - Peptidyl-prolyl cis-trans isomerase PpiD (EC 5.2.1.8), at 840,991 to 841,959
_733
Position (kb) 840
841 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 839.337 kb on + strand, within Pf6N2E2_730 at 839.338 kb on - strand, within Pf6N2E2_730 at 839.338 kb on - strand, within Pf6N2E2_730 at 839.338 kb on - strand, within Pf6N2E2_730 at 839.361 kb on + strand, within Pf6N2E2_730 at 839.361 kb on + strand, within Pf6N2E2_730 at 839.361 kb on + strand, within Pf6N2E2_730 at 839.362 kb on - strand, within Pf6N2E2_730 at 839.362 kb on - strand, within Pf6N2E2_730 at 839.362 kb on - strand, within Pf6N2E2_730 at 839.380 kb on + strand, within Pf6N2E2_730 at 839.880 kb on + strand, within Pf6N2E2_731 at 839.880 kb on + strand, within Pf6N2E2_731 at 839.881 kb on - strand, within Pf6N2E2_731 at 839.881 kb on - strand, within Pf6N2E2_731 at 839.881 kb on - strand, within Pf6N2E2_731 at 839.888 kb on + strand, within Pf6N2E2_731 at 839.888 kb on + strand, within Pf6N2E2_731 at 839.888 kb on + strand, within Pf6N2E2_731 at 839.888 kb on + strand, within Pf6N2E2_731 at 839.888 kb on + strand, within Pf6N2E2_731 at 839.888 kb on + strand, within Pf6N2E2_731 at 839.888 kb on + strand, within Pf6N2E2_731 at 840.298 kb on + strand at 840.298 kb on + strand at 840.305 kb on + strand at 840.305 kb on + strand at 840.306 kb on - strand at 840.306 kb on - strand at 840.396 kb on + strand, within Pf6N2E2_732 at 840.397 kb on - strand, within Pf6N2E2_732 at 840.397 kb on - strand, within Pf6N2E2_732 at 840.528 kb on + strand, within Pf6N2E2_732 at 840.528 kb on + strand, within Pf6N2E2_732 at 840.528 kb on + strand, within Pf6N2E2_732 at 840.528 kb on + strand, within Pf6N2E2_732 at 840.528 kb on + strand, within Pf6N2E2_732 at 840.528 kb on + strand, within Pf6N2E2_732 at 840.528 kb on + strand, within Pf6N2E2_732 at 840.528 kb on + strand, within Pf6N2E2_732 at 840.529 kb on - strand, within Pf6N2E2_732 at 840.529 kb on - strand, within Pf6N2E2_732 at 840.529 kb on - strand, within Pf6N2E2_732 at 840.529 kb on - strand, within Pf6N2E2_732 at 840.529 kb on - strand, within Pf6N2E2_732 at 840.529 kb on - strand, within Pf6N2E2_732 at 840.740 kb on + strand, within Pf6N2E2_732 at 840.852 kb on + strand, within Pf6N2E2_732 at 840.852 kb on + strand, within Pf6N2E2_732 at 840.853 kb on - strand, within Pf6N2E2_732 at 840.940 kb on - strand at 840.940 kb on - strand at 841.246 kb on + strand, within Pf6N2E2_733 at 841.247 kb on - strand, within Pf6N2E2_733 at 841.454 kb on - strand, within Pf6N2E2_733 at 841.454 kb on - strand, within Pf6N2E2_733 at 841.454 kb on - strand, within Pf6N2E2_733 at 841.882 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 839,337 + Pf6N2E2_730 0.86 +1.4 839,338 - Pf6N2E2_730 0.86 -1.1 839,338 - Pf6N2E2_730 0.86 +0.1 839,338 - Pf6N2E2_730 0.86 -0.6 839,361 + Pf6N2E2_730 0.88 +1.5 839,361 + Pf6N2E2_730 0.88 +0.4 839,361 + Pf6N2E2_730 0.88 +0.6 839,362 - Pf6N2E2_730 0.88 -0.1 839,362 - Pf6N2E2_730 0.88 -1.6 839,362 - Pf6N2E2_730 0.88 +0.3 839,380 + Pf6N2E2_730 0.89 +0.1 839,880 + Pf6N2E2_731 0.43 +0.4 839,880 + Pf6N2E2_731 0.43 +1.5 839,881 - Pf6N2E2_731 0.44 -2.4 839,881 - Pf6N2E2_731 0.44 -0.1 839,881 - Pf6N2E2_731 0.44 -1.6 839,888 + Pf6N2E2_731 0.44 +3.2 839,888 + Pf6N2E2_731 0.44 +0.4 839,888 + Pf6N2E2_731 0.44 +1.4 839,888 + Pf6N2E2_731 0.44 -0.1 839,888 + Pf6N2E2_731 0.44 -1.2 839,888 + Pf6N2E2_731 0.44 +1.0 839,888 + Pf6N2E2_731 0.44 -1.0 840,298 + -0.7 840,298 + +0.4 840,305 + +1.3 840,305 + -1.4 840,306 - -0.2 840,306 - -3.6 840,396 + Pf6N2E2_732 0.14 -1.8 840,397 - Pf6N2E2_732 0.14 -3.3 840,397 - Pf6N2E2_732 0.14 -0.4 840,528 + Pf6N2E2_732 0.33 -3.5 840,528 + Pf6N2E2_732 0.33 -3.0 840,528 + Pf6N2E2_732 0.33 -0.8 840,528 + Pf6N2E2_732 0.33 -0.1 840,528 + Pf6N2E2_732 0.33 -0.3 840,528 + Pf6N2E2_732 0.33 -0.7 840,528 + Pf6N2E2_732 0.33 -1.1 840,528 + Pf6N2E2_732 0.33 -1.4 840,529 - Pf6N2E2_732 0.34 -2.0 840,529 - Pf6N2E2_732 0.34 -0.7 840,529 - Pf6N2E2_732 0.34 +0.4 840,529 - Pf6N2E2_732 0.34 -0.0 840,529 - Pf6N2E2_732 0.34 +1.0 840,529 - Pf6N2E2_732 0.34 -1.4 840,740 + Pf6N2E2_732 0.65 +0.6 840,852 + Pf6N2E2_732 0.81 +0.6 840,852 + Pf6N2E2_732 0.81 +0.8 840,853 - Pf6N2E2_732 0.81 -0.6 840,940 - -0.6 840,940 - -1.6 841,246 + Pf6N2E2_733 0.26 -0.6 841,247 - Pf6N2E2_733 0.26 -2.1 841,454 - Pf6N2E2_733 0.48 -2.0 841,454 - Pf6N2E2_733 0.48 +0.1 841,454 - Pf6N2E2_733 0.48 +1.6 841,882 + -1.1
Or see this region's nucleotide sequence