Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5395 and Pf6N2E2_5396 are separated by 128 nucleotides Pf6N2E2_5396 and Pf6N2E2_5397 are separated by 89 nucleotides Pf6N2E2_5397 and Pf6N2E2_5398 are separated by 232 nucleotides Pf6N2E2_5398 and Pf6N2E2_5399 are separated by 1 nucleotides
Pf6N2E2_5395 - Arginine/ornithine ABC transporter, periplasmic arginine/ornithine binding protein, at 6,200,942 to 6,201,676
_5395
Pf6N2E2_5396 - YheO-like PAS domain, at 6,201,805 to 6,202,431
_5396
Pf6N2E2_5397 - Sarcosine oxidase beta subunit (EC 1.5.3.1), at 6,202,521 to 6,203,477
_5397
Pf6N2E2_5398 - Ornithine cyclodeaminase (EC 4.3.1.12), at 6,203,710 to 6,203,892
_5398
Pf6N2E2_5399 - Ornithine cyclodeaminase (EC 4.3.1.12), at 6,203,894 to 6,204,661
_5399
Position (kb)
6202
6203
6204 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6201.527 kb on + strand, within Pf6N2E2_5395 at 6201.528 kb on - strand, within Pf6N2E2_5395 at 6201.626 kb on + strand at 6201.626 kb on + strand at 6201.626 kb on + strand at 6201.626 kb on + strand at 6201.647 kb on + strand at 6201.647 kb on + strand at 6201.647 kb on + strand at 6201.647 kb on + strand at 6201.665 kb on + strand at 6201.665 kb on + strand at 6201.666 kb on - strand at 6201.677 kb on + strand at 6201.677 kb on + strand at 6201.678 kb on - strand at 6201.678 kb on - strand at 6201.728 kb on + strand at 6201.728 kb on + strand at 6201.728 kb on + strand at 6201.728 kb on + strand at 6201.728 kb on + strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.746 kb on + strand at 6201.747 kb on - strand at 6201.833 kb on + strand at 6201.833 kb on + strand at 6201.833 kb on + strand at 6201.834 kb on - strand at 6201.834 kb on - strand at 6201.852 kb on + strand at 6201.872 kb on + strand, within Pf6N2E2_5396 at 6201.872 kb on + strand, within Pf6N2E2_5396 at 6201.872 kb on + strand, within Pf6N2E2_5396 at 6201.872 kb on + strand, within Pf6N2E2_5396 at 6201.872 kb on + strand, within Pf6N2E2_5396 at 6201.872 kb on + strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6202.110 kb on + strand, within Pf6N2E2_5396 at 6202.111 kb on - strand, within Pf6N2E2_5396 at 6202.162 kb on + strand, within Pf6N2E2_5396 at 6202.163 kb on - strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.225 kb on - strand, within Pf6N2E2_5396 at 6202.225 kb on - strand, within Pf6N2E2_5396 at 6202.225 kb on - strand, within Pf6N2E2_5396 at 6202.225 kb on - strand, within Pf6N2E2_5396 at 6202.225 kb on - strand, within Pf6N2E2_5396 at 6202.225 kb on - strand, within Pf6N2E2_5396 at 6202.225 kb on - strand, within Pf6N2E2_5396 at 6202.297 kb on - strand, within Pf6N2E2_5396 at 6202.307 kb on + strand, within Pf6N2E2_5396 at 6202.307 kb on + strand, within Pf6N2E2_5396 at 6202.308 kb on - strand, within Pf6N2E2_5396 at 6202.308 kb on - strand, within Pf6N2E2_5396 at 6202.308 kb on - strand, within Pf6N2E2_5396 at 6202.308 kb on - strand, within Pf6N2E2_5396 at 6202.358 kb on + strand, within Pf6N2E2_5396 at 6202.358 kb on + strand, within Pf6N2E2_5396 at 6202.722 kb on + strand, within Pf6N2E2_5397 at 6202.723 kb on - strand, within Pf6N2E2_5397 at 6202.795 kb on + strand, within Pf6N2E2_5397 at 6202.796 kb on - strand, within Pf6N2E2_5397 at 6202.796 kb on - strand, within Pf6N2E2_5397 at 6202.796 kb on - strand, within Pf6N2E2_5397 at 6202.796 kb on - strand, within Pf6N2E2_5397 at 6202.830 kb on + strand, within Pf6N2E2_5397 at 6202.830 kb on + strand, within Pf6N2E2_5397 at 6202.830 kb on + strand, within Pf6N2E2_5397 at 6202.831 kb on - strand, within Pf6N2E2_5397 at 6202.831 kb on - strand, within Pf6N2E2_5397 at 6202.831 kb on - strand, within Pf6N2E2_5397 at 6202.831 kb on - strand, within Pf6N2E2_5397 at 6202.898 kb on + strand, within Pf6N2E2_5397 at 6202.898 kb on + strand, within Pf6N2E2_5397 at 6202.898 kb on + strand, within Pf6N2E2_5397 at 6202.899 kb on - strand, within Pf6N2E2_5397 at 6202.899 kb on - strand, within Pf6N2E2_5397 at 6202.899 kb on - strand, within Pf6N2E2_5397 at 6202.899 kb on - strand, within Pf6N2E2_5397 at 6202.899 kb on - strand, within Pf6N2E2_5397 at 6202.899 kb on - strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6203.178 kb on - strand, within Pf6N2E2_5397 at 6203.178 kb on - strand, within Pf6N2E2_5397 at 6203.188 kb on + strand, within Pf6N2E2_5397 at 6203.188 kb on + strand, within Pf6N2E2_5397 at 6203.189 kb on - strand, within Pf6N2E2_5397 at 6203.237 kb on - strand, within Pf6N2E2_5397 at 6203.237 kb on - strand, within Pf6N2E2_5397 at 6203.237 kb on - strand, within Pf6N2E2_5397 at 6203.237 kb on - strand, within Pf6N2E2_5397 at 6203.237 kb on - strand, within Pf6N2E2_5397 at 6203.237 kb on - strand, within Pf6N2E2_5397 at 6203.237 kb on - strand, within Pf6N2E2_5397 at 6203.342 kb on - strand, within Pf6N2E2_5397 at 6203.502 kb on + strand at 6203.504 kb on + strand at 6203.504 kb on + strand at 6203.504 kb on + strand at 6203.504 kb on + strand at 6203.505 kb on - strand at 6203.505 kb on - strand at 6203.505 kb on - strand at 6203.535 kb on - strand at 6203.535 kb on - strand at 6203.535 kb on - strand at 6203.566 kb on + strand at 6203.566 kb on + strand at 6203.566 kb on + strand at 6203.566 kb on + strand at 6203.566 kb on + strand at 6203.566 kb on + strand at 6203.566 kb on + strand at 6203.566 kb on + strand at 6203.566 kb on + strand at 6203.566 kb on + strand at 6203.993 kb on - strand, within Pf6N2E2_5399 at 6203.993 kb on - strand, within Pf6N2E2_5399 at 6204.186 kb on + strand, within Pf6N2E2_5399 at 6204.187 kb on - strand, within Pf6N2E2_5399 at 6204.225 kb on + strand, within Pf6N2E2_5399 at 6204.225 kb on + strand, within Pf6N2E2_5399 at 6204.225 kb on + strand, within Pf6N2E2_5399 at 6204.225 kb on + strand, within Pf6N2E2_5399 at 6204.225 kb on + strand, within Pf6N2E2_5399 at 6204.225 kb on + strand, within Pf6N2E2_5399 at 6204.225 kb on + strand, within Pf6N2E2_5399 at 6204.225 kb on + strand, within Pf6N2E2_5399 at 6204.226 kb on - strand, within Pf6N2E2_5399 at 6204.226 kb on - strand, within Pf6N2E2_5399 at 6204.226 kb on - strand, within Pf6N2E2_5399 at 6204.438 kb on + strand, within Pf6N2E2_5399 at 6204.438 kb on + strand, within Pf6N2E2_5399 at 6204.438 kb on + strand, within Pf6N2E2_5399 at 6204.439 kb on - strand, within Pf6N2E2_5399 at 6204.439 kb on - strand, within Pf6N2E2_5399 at 6204.439 kb on - strand, within Pf6N2E2_5399 at 6204.459 kb on + strand, within Pf6N2E2_5399 at 6204.460 kb on - strand, within Pf6N2E2_5399 at 6204.460 kb on - strand, within Pf6N2E2_5399
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 6,201,527 + Pf6N2E2_5395 0.80 -0.1 6,201,528 - Pf6N2E2_5395 0.80 -1.5 6,201,626 + -0.4 6,201,626 + +0.6 6,201,626 + +0.6 6,201,626 + +0.5 6,201,647 + -0.4 6,201,647 + -1.1 6,201,647 + -0.4 6,201,647 + -0.8 6,201,665 + -1.6 6,201,665 + -0.7 6,201,666 - +0.1 6,201,677 + +1.1 6,201,677 + +0.7 6,201,678 - +0.8 6,201,678 - -2.4 6,201,728 + +2.0 6,201,728 + +1.8 6,201,728 + +0.8 6,201,728 + -1.4 6,201,728 + +1.2 6,201,729 - +0.6 6,201,729 - +0.7 6,201,729 - -1.5 6,201,729 - +0.6 6,201,729 - -0.8 6,201,729 - -0.9 6,201,729 - -1.6 6,201,729 - +0.1 6,201,729 - -0.8 6,201,729 - +0.7 6,201,746 + +1.2 6,201,747 - +1.8 6,201,833 + -1.9 6,201,833 + -0.8 6,201,833 + +1.2 6,201,834 - -1.8 6,201,834 - -1.1 6,201,852 + -0.3 6,201,872 + Pf6N2E2_5396 0.11 -0.4 6,201,872 + Pf6N2E2_5396 0.11 -0.6 6,201,872 + Pf6N2E2_5396 0.11 -0.2 6,201,872 + Pf6N2E2_5396 0.11 -0.5 6,201,872 + Pf6N2E2_5396 0.11 -2.7 6,201,872 + Pf6N2E2_5396 0.11 -0.8 6,201,873 - Pf6N2E2_5396 0.11 +0.0 6,201,873 - Pf6N2E2_5396 0.11 -0.6 6,201,873 - Pf6N2E2_5396 0.11 -2.3 6,201,873 - Pf6N2E2_5396 0.11 -1.2 6,201,873 - Pf6N2E2_5396 0.11 -1.1 6,201,873 - Pf6N2E2_5396 0.11 +0.1 6,201,873 - Pf6N2E2_5396 0.11 -1.3 6,201,873 - Pf6N2E2_5396 0.11 +0.4 6,201,873 - Pf6N2E2_5396 0.11 -1.5 6,201,873 - Pf6N2E2_5396 0.11 -0.5 6,201,873 - Pf6N2E2_5396 0.11 -3.3 6,201,873 - Pf6N2E2_5396 0.11 +0.2 6,202,110 + Pf6N2E2_5396 0.49 +0.3 6,202,111 - Pf6N2E2_5396 0.49 +0.1 6,202,162 + Pf6N2E2_5396 0.57 -0.4 6,202,163 - Pf6N2E2_5396 0.57 -0.8 6,202,224 + Pf6N2E2_5396 0.67 +0.7 6,202,224 + Pf6N2E2_5396 0.67 -0.7 6,202,224 + Pf6N2E2_5396 0.67 +1.1 6,202,224 + Pf6N2E2_5396 0.67 +0.4 6,202,224 + Pf6N2E2_5396 0.67 -0.4 6,202,224 + Pf6N2E2_5396 0.67 +0.6 6,202,224 + Pf6N2E2_5396 0.67 -2.0 6,202,224 + Pf6N2E2_5396 0.67 +0.2 6,202,224 + Pf6N2E2_5396 0.67 +0.2 6,202,225 - Pf6N2E2_5396 0.67 -0.8 6,202,225 - Pf6N2E2_5396 0.67 +0.2 6,202,225 - Pf6N2E2_5396 0.67 -2.4 6,202,225 - Pf6N2E2_5396 0.67 +1.3 6,202,225 - Pf6N2E2_5396 0.67 +0.4 6,202,225 - Pf6N2E2_5396 0.67 +1.4 6,202,225 - Pf6N2E2_5396 0.67 -0.5 6,202,297 - Pf6N2E2_5396 0.78 -1.0 6,202,307 + Pf6N2E2_5396 0.80 +0.2 6,202,307 + Pf6N2E2_5396 0.80 -1.8 6,202,308 - Pf6N2E2_5396 0.80 +0.6 6,202,308 - Pf6N2E2_5396 0.80 +0.7 6,202,308 - Pf6N2E2_5396 0.80 -0.5 6,202,308 - Pf6N2E2_5396 0.80 -0.4 6,202,358 + Pf6N2E2_5396 0.88 +0.1 6,202,358 + Pf6N2E2_5396 0.88 -2.4 6,202,722 + Pf6N2E2_5397 0.21 -0.8 6,202,723 - Pf6N2E2_5397 0.21 -0.5 6,202,795 + Pf6N2E2_5397 0.29 -2.3 6,202,796 - Pf6N2E2_5397 0.29 -0.0 6,202,796 - Pf6N2E2_5397 0.29 +0.4 6,202,796 - Pf6N2E2_5397 0.29 -0.8 6,202,796 - Pf6N2E2_5397 0.29 -1.4 6,202,830 + Pf6N2E2_5397 0.32 +0.2 6,202,830 + Pf6N2E2_5397 0.32 +1.1 6,202,830 + Pf6N2E2_5397 0.32 +1.2 6,202,831 - Pf6N2E2_5397 0.32 +0.0 6,202,831 - Pf6N2E2_5397 0.32 +2.1 6,202,831 - Pf6N2E2_5397 0.32 -1.8 6,202,831 - Pf6N2E2_5397 0.32 +0.2 6,202,898 + Pf6N2E2_5397 0.39 -0.1 6,202,898 + Pf6N2E2_5397 0.39 +0.8 6,202,898 + Pf6N2E2_5397 0.39 +0.6 6,202,899 - Pf6N2E2_5397 0.39 -0.2 6,202,899 - Pf6N2E2_5397 0.39 -0.0 6,202,899 - Pf6N2E2_5397 0.39 -1.1 6,202,899 - Pf6N2E2_5397 0.39 -0.2 6,202,899 - Pf6N2E2_5397 0.39 -0.8 6,202,899 - Pf6N2E2_5397 0.39 -0.9 6,202,900 + Pf6N2E2_5397 0.40 -1.0 6,202,900 + Pf6N2E2_5397 0.40 +1.0 6,202,900 + Pf6N2E2_5397 0.40 -2.1 6,202,900 + Pf6N2E2_5397 0.40 -0.5 6,202,900 + Pf6N2E2_5397 0.40 -1.6 6,202,900 + Pf6N2E2_5397 0.40 -0.9 6,202,900 + Pf6N2E2_5397 0.40 -3.4 6,202,900 + Pf6N2E2_5397 0.40 +0.4 6,202,900 + Pf6N2E2_5397 0.40 -0.8 6,202,900 + Pf6N2E2_5397 0.40 -0.4 6,202,901 - Pf6N2E2_5397 0.40 -0.1 6,202,901 - Pf6N2E2_5397 0.40 +0.2 6,202,901 - Pf6N2E2_5397 0.40 +1.2 6,202,901 - Pf6N2E2_5397 0.40 +1.4 6,202,901 - Pf6N2E2_5397 0.40 -0.1 6,202,901 - Pf6N2E2_5397 0.40 -2.5 6,202,901 - Pf6N2E2_5397 0.40 +0.4 6,202,901 - Pf6N2E2_5397 0.40 -1.4 6,202,901 - Pf6N2E2_5397 0.40 +1.2 6,203,178 - Pf6N2E2_5397 0.69 +0.5 6,203,178 - Pf6N2E2_5397 0.69 +1.0 6,203,188 + Pf6N2E2_5397 0.70 +0.2 6,203,188 + Pf6N2E2_5397 0.70 -2.7 6,203,189 - Pf6N2E2_5397 0.70 +1.7 6,203,237 - Pf6N2E2_5397 0.75 -0.9 6,203,237 - Pf6N2E2_5397 0.75 +0.4 6,203,237 - Pf6N2E2_5397 0.75 +1.2 6,203,237 - Pf6N2E2_5397 0.75 -1.8 6,203,237 - Pf6N2E2_5397 0.75 -1.0 6,203,237 - Pf6N2E2_5397 0.75 -0.3 6,203,237 - Pf6N2E2_5397 0.75 +2.3 6,203,342 - Pf6N2E2_5397 0.86 +1.2 6,203,502 + -1.8 6,203,504 + -1.6 6,203,504 + +1.4 6,203,504 + +0.1 6,203,504 + +0.2 6,203,505 - +0.2 6,203,505 - +1.0 6,203,505 - -0.7 6,203,535 - -2.9 6,203,535 - -1.6 6,203,535 - -1.0 6,203,566 + -1.4 6,203,566 + -0.4 6,203,566 + +0.6 6,203,566 + +0.6 6,203,566 + +0.4 6,203,566 + +1.6 6,203,566 + -1.6 6,203,566 + +1.5 6,203,566 + -0.4 6,203,566 + +0.6 6,203,993 - Pf6N2E2_5399 0.13 +0.4 6,203,993 - Pf6N2E2_5399 0.13 -1.4 6,204,186 + Pf6N2E2_5399 0.38 -0.2 6,204,187 - Pf6N2E2_5399 0.38 +0.3 6,204,225 + Pf6N2E2_5399 0.43 +0.2 6,204,225 + Pf6N2E2_5399 0.43 +0.9 6,204,225 + Pf6N2E2_5399 0.43 -2.8 6,204,225 + Pf6N2E2_5399 0.43 +1.1 6,204,225 + Pf6N2E2_5399 0.43 -0.8 6,204,225 + Pf6N2E2_5399 0.43 -0.6 6,204,225 + Pf6N2E2_5399 0.43 +0.9 6,204,225 + Pf6N2E2_5399 0.43 -1.0 6,204,226 - Pf6N2E2_5399 0.43 -0.1 6,204,226 - Pf6N2E2_5399 0.43 +0.2 6,204,226 - Pf6N2E2_5399 0.43 +1.2 6,204,438 + Pf6N2E2_5399 0.71 +0.1 6,204,438 + Pf6N2E2_5399 0.71 +0.3 6,204,438 + Pf6N2E2_5399 0.71 -0.8 6,204,439 - Pf6N2E2_5399 0.71 +0.9 6,204,439 - Pf6N2E2_5399 0.71 +0.3 6,204,439 - Pf6N2E2_5399 0.71 -0.6 6,204,459 + Pf6N2E2_5399 0.74 -0.6 6,204,460 - Pf6N2E2_5399 0.74 -1.0 6,204,460 - Pf6N2E2_5399 0.74 -1.3
Or see this region's nucleotide sequence