Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5103 and Pf6N2E2_5104 are separated by 9 nucleotides Pf6N2E2_5104 and Pf6N2E2_5105 are separated by 80 nucleotides
Pf6N2E2_5103 - Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA, at 5,884,996 to 5,886,114
_5103
Pf6N2E2_5104 - Conserved uncharacterized protein CreA, at 5,886,124 to 5,886,579
_5104
Pf6N2E2_5105 - hypothetical protein, at 5,886,660 to 5,888,699
_5105
Position (kb)
5886
5887 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5885.846 kb on + strand, within Pf6N2E2_5103 at 5885.846 kb on + strand, within Pf6N2E2_5103 at 5886.287 kb on - strand, within Pf6N2E2_5104 at 5886.381 kb on + strand, within Pf6N2E2_5104 at 5886.381 kb on + strand, within Pf6N2E2_5104 at 5886.381 kb on + strand, within Pf6N2E2_5104 at 5886.489 kb on + strand, within Pf6N2E2_5104 at 5886.489 kb on + strand, within Pf6N2E2_5104 at 5886.490 kb on - strand, within Pf6N2E2_5104 at 5886.490 kb on - strand, within Pf6N2E2_5104 at 5886.500 kb on - strand, within Pf6N2E2_5104 at 5886.500 kb on - strand, within Pf6N2E2_5104 at 5886.508 kb on + strand, within Pf6N2E2_5104 at 5886.508 kb on + strand, within Pf6N2E2_5104 at 5886.577 kb on + strand at 5886.578 kb on - strand at 5886.617 kb on + strand at 5886.621 kb on + strand at 5886.622 kb on - strand at 5886.659 kb on + strand at 5886.660 kb on - strand at 5886.660 kb on - strand at 5886.685 kb on + strand at 5886.730 kb on - strand at 5886.760 kb on + strand at 5886.797 kb on + strand at 5886.820 kb on + strand at 5886.820 kb on + strand at 5886.820 kb on + strand at 5886.826 kb on - strand at 5886.826 kb on - strand at 5886.876 kb on + strand, within Pf6N2E2_5105 at 5886.876 kb on + strand, within Pf6N2E2_5105 at 5886.876 kb on + strand, within Pf6N2E2_5105 at 5886.877 kb on - strand, within Pf6N2E2_5105 at 5886.906 kb on + strand, within Pf6N2E2_5105 at 5886.906 kb on + strand, within Pf6N2E2_5105 at 5886.907 kb on - strand, within Pf6N2E2_5105 at 5887.120 kb on - strand, within Pf6N2E2_5105 at 5887.194 kb on + strand, within Pf6N2E2_5105 at 5887.194 kb on + strand, within Pf6N2E2_5105 at 5887.261 kb on - strand, within Pf6N2E2_5105 at 5887.308 kb on + strand, within Pf6N2E2_5105 at 5887.308 kb on + strand, within Pf6N2E2_5105 at 5887.308 kb on + strand, within Pf6N2E2_5105 at 5887.308 kb on + strand, within Pf6N2E2_5105 at 5887.309 kb on - strand, within Pf6N2E2_5105 at 5887.309 kb on - strand, within Pf6N2E2_5105 at 5887.309 kb on - strand, within Pf6N2E2_5105 at 5887.350 kb on + strand, within Pf6N2E2_5105 at 5887.392 kb on + strand, within Pf6N2E2_5105 at 5887.393 kb on - strand, within Pf6N2E2_5105 at 5887.393 kb on - strand, within Pf6N2E2_5105 at 5887.393 kb on - strand, within Pf6N2E2_5105 at 5887.393 kb on - strand, within Pf6N2E2_5105 at 5887.458 kb on + strand, within Pf6N2E2_5105 at 5887.458 kb on + strand, within Pf6N2E2_5105 at 5887.458 kb on + strand, within Pf6N2E2_5105 at 5887.458 kb on + strand, within Pf6N2E2_5105 at 5887.459 kb on - strand, within Pf6N2E2_5105 at 5887.459 kb on - strand, within Pf6N2E2_5105 at 5887.459 kb on - strand, within Pf6N2E2_5105 at 5887.459 kb on - strand, within Pf6N2E2_5105 at 5887.459 kb on - strand, within Pf6N2E2_5105 at 5887.459 kb on - strand, within Pf6N2E2_5105 at 5887.459 kb on - strand, within Pf6N2E2_5105 at 5887.527 kb on + strand, within Pf6N2E2_5105 at 5887.527 kb on + strand, within Pf6N2E2_5105 at 5887.527 kb on + strand, within Pf6N2E2_5105 at 5887.527 kb on + strand, within Pf6N2E2_5105 at 5887.527 kb on + strand, within Pf6N2E2_5105 at 5887.527 kb on + strand, within Pf6N2E2_5105 at 5887.527 kb on + strand, within Pf6N2E2_5105 at 5887.528 kb on - strand, within Pf6N2E2_5105 at 5887.528 kb on - strand, within Pf6N2E2_5105 at 5887.528 kb on - strand, within Pf6N2E2_5105 at 5887.528 kb on - strand, within Pf6N2E2_5105 at 5887.528 kb on - strand, within Pf6N2E2_5105
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 5,885,846 + Pf6N2E2_5103 0.76 +1.9 5,885,846 + Pf6N2E2_5103 0.76 -0.7 5,886,287 - Pf6N2E2_5104 0.36 -2.1 5,886,381 + Pf6N2E2_5104 0.56 -1.3 5,886,381 + Pf6N2E2_5104 0.56 -0.2 5,886,381 + Pf6N2E2_5104 0.56 +0.3 5,886,489 + Pf6N2E2_5104 0.80 -1.6 5,886,489 + Pf6N2E2_5104 0.80 -1.2 5,886,490 - Pf6N2E2_5104 0.80 -2.3 5,886,490 - Pf6N2E2_5104 0.80 -0.6 5,886,500 - Pf6N2E2_5104 0.82 +0.3 5,886,500 - Pf6N2E2_5104 0.82 -0.8 5,886,508 + Pf6N2E2_5104 0.84 -1.2 5,886,508 + Pf6N2E2_5104 0.84 -0.3 5,886,577 + -0.3 5,886,578 - -0.7 5,886,617 + -0.5 5,886,621 + +1.2 5,886,622 - +0.2 5,886,659 + -2.1 5,886,660 - -3.4 5,886,660 - +0.4 5,886,685 + -1.7 5,886,730 - +1.0 5,886,760 + -1.5 5,886,797 + -0.6 5,886,820 + +0.4 5,886,820 + +0.5 5,886,820 + +0.0 5,886,826 - -0.1 5,886,826 - +0.3 5,886,876 + Pf6N2E2_5105 0.11 +0.7 5,886,876 + Pf6N2E2_5105 0.11 +0.0 5,886,876 + Pf6N2E2_5105 0.11 +0.9 5,886,877 - Pf6N2E2_5105 0.11 +0.3 5,886,906 + Pf6N2E2_5105 0.12 +0.5 5,886,906 + Pf6N2E2_5105 0.12 +1.9 5,886,907 - Pf6N2E2_5105 0.12 +1.2 5,887,120 - Pf6N2E2_5105 0.23 -0.4 5,887,194 + Pf6N2E2_5105 0.26 +1.0 5,887,194 + Pf6N2E2_5105 0.26 +1.2 5,887,261 - Pf6N2E2_5105 0.29 -3.4 5,887,308 + Pf6N2E2_5105 0.32 +0.6 5,887,308 + Pf6N2E2_5105 0.32 +1.6 5,887,308 + Pf6N2E2_5105 0.32 +1.7 5,887,308 + Pf6N2E2_5105 0.32 -0.7 5,887,309 - Pf6N2E2_5105 0.32 -0.3 5,887,309 - Pf6N2E2_5105 0.32 +2.9 5,887,309 - Pf6N2E2_5105 0.32 +0.2 5,887,350 + Pf6N2E2_5105 0.34 -1.3 5,887,392 + Pf6N2E2_5105 0.36 -0.8 5,887,393 - Pf6N2E2_5105 0.36 +2.3 5,887,393 - Pf6N2E2_5105 0.36 +0.2 5,887,393 - Pf6N2E2_5105 0.36 +0.1 5,887,393 - Pf6N2E2_5105 0.36 -1.5 5,887,458 + Pf6N2E2_5105 0.39 +0.3 5,887,458 + Pf6N2E2_5105 0.39 +1.5 5,887,458 + Pf6N2E2_5105 0.39 -0.3 5,887,458 + Pf6N2E2_5105 0.39 -0.3 5,887,459 - Pf6N2E2_5105 0.39 +0.0 5,887,459 - Pf6N2E2_5105 0.39 +1.2 5,887,459 - Pf6N2E2_5105 0.39 +1.3 5,887,459 - Pf6N2E2_5105 0.39 +0.2 5,887,459 - Pf6N2E2_5105 0.39 -2.7 5,887,459 - Pf6N2E2_5105 0.39 +1.3 5,887,459 - Pf6N2E2_5105 0.39 +1.2 5,887,527 + Pf6N2E2_5105 0.42 +0.7 5,887,527 + Pf6N2E2_5105 0.42 -0.1 5,887,527 + Pf6N2E2_5105 0.42 -2.0 5,887,527 + Pf6N2E2_5105 0.42 +2.6 5,887,527 + Pf6N2E2_5105 0.42 -0.1 5,887,527 + Pf6N2E2_5105 0.42 -2.0 5,887,527 + Pf6N2E2_5105 0.42 +0.3 5,887,528 - Pf6N2E2_5105 0.43 -0.7 5,887,528 - Pf6N2E2_5105 0.43 +1.7 5,887,528 - Pf6N2E2_5105 0.43 -0.3 5,887,528 - Pf6N2E2_5105 0.43 -0.3 5,887,528 - Pf6N2E2_5105 0.43 -0.1
Or see this region's nucleotide sequence