Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5017

Experiment: RCH2 defined with Cobalt chloride 0.04 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5015 and Pf6N2E2_5016 are separated by 40 nucleotidesPf6N2E2_5016 and Pf6N2E2_5017 overlap by 8 nucleotidesPf6N2E2_5017 and Pf6N2E2_5018 are separated by 215 nucleotides Pf6N2E2_5015 - Magnesium and cobalt efflux protein CorC, at 5,782,132 to 5,782,971 _5015 Pf6N2E2_5016 - Metal-dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly, at 5,783,012 to 5,783,470 _5016 Pf6N2E2_5017 - Phosphate starvation-inducible protein PhoH, predicted ATPase, at 5,783,463 to 5,784,473 _5017 Pf6N2E2_5018 - tRNA-i(6)A37 methylthiotransferase, at 5,784,689 to 5,786,053 _5018 Position (kb) 5783 5784 5785Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4 5at 5783.011 kb on - strandat 5783.011 kb on - strandat 5783.011 kb on - strandat 5783.011 kb on - strandat 5783.011 kb on - strandat 5783.011 kb on - strandat 5783.011 kb on - strandat 5783.011 kb on - strandat 5783.807 kb on - strand, within Pf6N2E2_5017at 5783.807 kb on - strand, within Pf6N2E2_5017at 5783.807 kb on - strand, within Pf6N2E2_5017at 5783.807 kb on - strand, within Pf6N2E2_5017at 5783.807 kb on - strand, within Pf6N2E2_5017at 5783.891 kb on - strand, within Pf6N2E2_5017at 5783.906 kb on - strand, within Pf6N2E2_5017at 5783.906 kb on - strand, within Pf6N2E2_5017at 5784.041 kb on - strand, within Pf6N2E2_5017at 5784.041 kb on - strand, within Pf6N2E2_5017at 5784.041 kb on - strand, within Pf6N2E2_5017at 5784.107 kb on - strand, within Pf6N2E2_5017at 5784.107 kb on - strand, within Pf6N2E2_5017at 5784.107 kb on - strand, within Pf6N2E2_5017at 5784.502 kb on - strandat 5784.528 kb on - strandat 5784.548 kb on + strandat 5784.548 kb on + strandat 5784.634 kb on + strandat 5784.635 kb on - strandat 5784.635 kb on - strandat 5785.005 kb on + strand, within Pf6N2E2_5018at 5785.005 kb on + strand, within Pf6N2E2_5018at 5785.006 kb on - strand, within Pf6N2E2_5018at 5785.006 kb on - strand, within Pf6N2E2_5018at 5785.017 kb on + strand, within Pf6N2E2_5018at 5785.017 kb on + strand, within Pf6N2E2_5018at 5785.018 kb on - strand, within Pf6N2E2_5018at 5785.018 kb on - strand, within Pf6N2E2_5018at 5785.018 kb on - strand, within Pf6N2E2_5018at 5785.100 kb on - strand, within Pf6N2E2_5018at 5785.100 kb on - strand, within Pf6N2E2_5018at 5785.161 kb on + strand, within Pf6N2E2_5018

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM
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5,783,011 - -4.2
5,783,011 - -2.9
5,783,011 - -2.9
5,783,011 - -3.4
5,783,011 - -1.5
5,783,011 - -2.7
5,783,011 - -3.5
5,783,011 - -2.9
5,783,807 - Pf6N2E2_5017 0.34 +0.3
5,783,807 - Pf6N2E2_5017 0.34 -1.5
5,783,807 - Pf6N2E2_5017 0.34 +2.7
5,783,807 - Pf6N2E2_5017 0.34 -0.5
5,783,807 - Pf6N2E2_5017 0.34 +1.3
5,783,891 - Pf6N2E2_5017 0.42 -0.2
5,783,906 - Pf6N2E2_5017 0.44 +3.8
5,783,906 - Pf6N2E2_5017 0.44 -1.7
5,784,041 - Pf6N2E2_5017 0.57 -0.9
5,784,041 - Pf6N2E2_5017 0.57 +4.0
5,784,041 - Pf6N2E2_5017 0.57 +2.8
5,784,107 - Pf6N2E2_5017 0.64 +1.5
5,784,107 - Pf6N2E2_5017 0.64 +4.6
5,784,107 - Pf6N2E2_5017 0.64 +1.3
5,784,502 - -1.8
5,784,528 - -0.7
5,784,548 + +4.7
5,784,548 + +5.1
5,784,634 + +2.5
5,784,635 - -2.1
5,784,635 - -0.2
5,785,005 + Pf6N2E2_5018 0.23 +0.7
5,785,005 + Pf6N2E2_5018 0.23 +0.7
5,785,006 - Pf6N2E2_5018 0.23 -0.7
5,785,006 - Pf6N2E2_5018 0.23 -1.9
5,785,017 + Pf6N2E2_5018 0.24 -2.9
5,785,017 + Pf6N2E2_5018 0.24 +0.3
5,785,018 - Pf6N2E2_5018 0.24 -0.1
5,785,018 - Pf6N2E2_5018 0.24 -0.8
5,785,018 - Pf6N2E2_5018 0.24 -3.1
5,785,100 - Pf6N2E2_5018 0.30 +0.0
5,785,100 - Pf6N2E2_5018 0.30 +0.1
5,785,161 + Pf6N2E2_5018 0.35 -0.6

Or see this region's nucleotide sequence