Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4432 and Pf6N2E2_4433 overlap by 4 nucleotides Pf6N2E2_4433 and Pf6N2E2_4434 are separated by 396 nucleotides
Pf6N2E2_4432 - Uroporphyrinogen-III synthase (EC 4.2.1.75), at 5,163,293 to 5,165,209
_4432
Pf6N2E2_4433 - Uncharacterized protein EC-HemY, likely associated with heme metabolism based on gene clustering with hemC, hemD in Proteobacteria (unrelated to HemY-type PPO in GramPositives), at 5,165,206 to 5,166,453
_4433
Pf6N2E2_4434 - Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation, at 5,166,850 to 5,167,392
_4434
Position (kb) 5165
5166
5167 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5164.262 kb on - strand, within Pf6N2E2_4432 at 5164.739 kb on + strand, within Pf6N2E2_4432 at 5164.739 kb on + strand, within Pf6N2E2_4432 at 5164.740 kb on - strand, within Pf6N2E2_4432 at 5164.740 kb on - strand, within Pf6N2E2_4432 at 5164.830 kb on - strand, within Pf6N2E2_4432 at 5164.831 kb on + strand, within Pf6N2E2_4432 at 5164.968 kb on - strand, within Pf6N2E2_4432 at 5165.129 kb on + strand at 5165.130 kb on - strand at 5165.130 kb on - strand at 5165.218 kb on + strand at 5165.218 kb on + strand at 5165.219 kb on - strand at 5165.287 kb on + strand at 5165.288 kb on - strand at 5165.302 kb on + strand at 5165.406 kb on + strand, within Pf6N2E2_4433 at 5165.406 kb on + strand, within Pf6N2E2_4433 at 5165.406 kb on + strand, within Pf6N2E2_4433 at 5165.407 kb on - strand, within Pf6N2E2_4433 at 5165.407 kb on - strand, within Pf6N2E2_4433 at 5165.566 kb on + strand, within Pf6N2E2_4433 at 5165.566 kb on + strand, within Pf6N2E2_4433 at 5165.566 kb on + strand, within Pf6N2E2_4433 at 5165.566 kb on + strand, within Pf6N2E2_4433 at 5165.567 kb on - strand, within Pf6N2E2_4433 at 5165.567 kb on - strand, within Pf6N2E2_4433 at 5165.567 kb on - strand, within Pf6N2E2_4433 at 5165.567 kb on - strand, within Pf6N2E2_4433 at 5165.569 kb on + strand, within Pf6N2E2_4433 at 5165.570 kb on - strand, within Pf6N2E2_4433 at 5165.608 kb on + strand, within Pf6N2E2_4433 at 5165.608 kb on + strand, within Pf6N2E2_4433 at 5165.609 kb on - strand, within Pf6N2E2_4433 at 5165.609 kb on - strand, within Pf6N2E2_4433 at 5166.028 kb on + strand, within Pf6N2E2_4433 at 5166.028 kb on + strand, within Pf6N2E2_4433 at 5166.028 kb on + strand, within Pf6N2E2_4433 at 5166.029 kb on - strand, within Pf6N2E2_4433 at 5166.077 kb on + strand, within Pf6N2E2_4433 at 5166.077 kb on + strand, within Pf6N2E2_4433 at 5166.078 kb on - strand, within Pf6N2E2_4433 at 5166.103 kb on + strand, within Pf6N2E2_4433 at 5166.103 kb on + strand, within Pf6N2E2_4433 at 5166.104 kb on - strand, within Pf6N2E2_4433 at 5166.127 kb on + strand, within Pf6N2E2_4433 at 5166.271 kb on + strand, within Pf6N2E2_4433 at 5166.482 kb on + strand at 5166.482 kb on + strand at 5166.482 kb on + strand at 5166.483 kb on - strand at 5166.483 kb on - strand at 5166.483 kb on - strand at 5166.508 kb on - strand at 5166.508 kb on - strand at 5166.537 kb on + strand at 5166.537 kb on + strand at 5166.537 kb on + strand at 5166.537 kb on + strand at 5166.537 kb on + strand at 5166.538 kb on - strand at 5166.538 kb on - strand at 5166.564 kb on - strand at 5166.588 kb on - strand at 5166.614 kb on + strand at 5166.615 kb on - strand at 5166.703 kb on - strand at 5166.711 kb on - strand at 5166.728 kb on - strand at 5166.755 kb on + strand at 5166.755 kb on + strand at 5166.756 kb on - strand at 5166.756 kb on - strand at 5166.818 kb on + strand at 5166.839 kb on - strand at 5166.839 kb on - strand at 5166.925 kb on + strand, within Pf6N2E2_4434 at 5166.925 kb on + strand, within Pf6N2E2_4434 at 5166.925 kb on + strand, within Pf6N2E2_4434 at 5166.934 kb on + strand, within Pf6N2E2_4434 at 5166.934 kb on + strand, within Pf6N2E2_4434 at 5166.998 kb on + strand, within Pf6N2E2_4434 at 5166.998 kb on + strand, within Pf6N2E2_4434 at 5166.998 kb on + strand, within Pf6N2E2_4434 at 5166.998 kb on + strand, within Pf6N2E2_4434 at 5166.998 kb on + strand, within Pf6N2E2_4434 at 5166.998 kb on + strand, within Pf6N2E2_4434 at 5166.999 kb on - strand, within Pf6N2E2_4434 at 5167.022 kb on + strand, within Pf6N2E2_4434 at 5167.022 kb on + strand, within Pf6N2E2_4434 at 5167.022 kb on + strand, within Pf6N2E2_4434 at 5167.022 kb on + strand, within Pf6N2E2_4434 at 5167.022 kb on + strand, within Pf6N2E2_4434 at 5167.022 kb on + strand, within Pf6N2E2_4434 at 5167.022 kb on + strand, within Pf6N2E2_4434 at 5167.023 kb on - strand, within Pf6N2E2_4434 at 5167.023 kb on - strand, within Pf6N2E2_4434 at 5167.054 kb on + strand, within Pf6N2E2_4434 at 5167.054 kb on + strand, within Pf6N2E2_4434 at 5167.054 kb on + strand, within Pf6N2E2_4434 at 5167.054 kb on + strand, within Pf6N2E2_4434 at 5167.054 kb on + strand, within Pf6N2E2_4434 at 5167.055 kb on - strand, within Pf6N2E2_4434 at 5167.055 kb on - strand, within Pf6N2E2_4434 at 5167.055 kb on - strand, within Pf6N2E2_4434 at 5167.230 kb on + strand, within Pf6N2E2_4434 at 5167.231 kb on - strand, within Pf6N2E2_4434 at 5167.257 kb on + strand, within Pf6N2E2_4434 at 5167.309 kb on + strand, within Pf6N2E2_4434 at 5167.309 kb on + strand, within Pf6N2E2_4434 at 5167.310 kb on - strand, within Pf6N2E2_4434 at 5167.313 kb on + strand, within Pf6N2E2_4434 at 5167.313 kb on + strand, within Pf6N2E2_4434 at 5167.313 kb on + strand, within Pf6N2E2_4434 at 5167.313 kb on + strand, within Pf6N2E2_4434 at 5167.313 kb on + strand, within Pf6N2E2_4434 at 5167.313 kb on + strand, within Pf6N2E2_4434 at 5167.313 kb on + strand, within Pf6N2E2_4434 at 5167.314 kb on - strand, within Pf6N2E2_4434 at 5167.314 kb on - strand, within Pf6N2E2_4434 at 5167.314 kb on - strand, within Pf6N2E2_4434 at 5167.314 kb on - strand, within Pf6N2E2_4434 at 5167.314 kb on - strand, within Pf6N2E2_4434 at 5167.314 kb on - strand, within Pf6N2E2_4434 at 5167.363 kb on - strand at 5167.400 kb on + strand at 5167.400 kb on + strand at 5167.400 kb on + strand at 5167.401 kb on - strand at 5167.401 kb on - strand at 5167.401 kb on - strand at 5167.401 kb on - strand at 5167.401 kb on - strand at 5167.409 kb on + strand at 5167.410 kb on - strand at 5167.410 kb on - strand at 5167.420 kb on + strand at 5167.420 kb on + strand at 5167.421 kb on - strand at 5167.447 kb on + strand at 5167.447 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 5,164,262 - Pf6N2E2_4432 0.51 -0.9 5,164,739 + Pf6N2E2_4432 0.75 -1.2 5,164,739 + Pf6N2E2_4432 0.75 -1.9 5,164,740 - Pf6N2E2_4432 0.75 -0.9 5,164,740 - Pf6N2E2_4432 0.75 -4.2 5,164,830 - Pf6N2E2_4432 0.80 -1.6 5,164,831 + Pf6N2E2_4432 0.80 -1.1 5,164,968 - Pf6N2E2_4432 0.87 -2.0 5,165,129 + +2.0 5,165,130 - +0.0 5,165,130 - -1.0 5,165,218 + -1.5 5,165,218 + -2.1 5,165,219 - -1.2 5,165,287 + -1.0 5,165,288 - -1.0 5,165,302 + -0.5 5,165,406 + Pf6N2E2_4433 0.16 -1.2 5,165,406 + Pf6N2E2_4433 0.16 -0.2 5,165,406 + Pf6N2E2_4433 0.16 -0.3 5,165,407 - Pf6N2E2_4433 0.16 -0.5 5,165,407 - Pf6N2E2_4433 0.16 +0.0 5,165,566 + Pf6N2E2_4433 0.29 -1.0 5,165,566 + Pf6N2E2_4433 0.29 -1.2 5,165,566 + Pf6N2E2_4433 0.29 -2.4 5,165,566 + Pf6N2E2_4433 0.29 -2.0 5,165,567 - Pf6N2E2_4433 0.29 -3.0 5,165,567 - Pf6N2E2_4433 0.29 +0.3 5,165,567 - Pf6N2E2_4433 0.29 -0.7 5,165,567 - Pf6N2E2_4433 0.29 -0.4 5,165,569 + Pf6N2E2_4433 0.29 -2.4 5,165,570 - Pf6N2E2_4433 0.29 -1.6 5,165,608 + Pf6N2E2_4433 0.32 -1.2 5,165,608 + Pf6N2E2_4433 0.32 -1.3 5,165,609 - Pf6N2E2_4433 0.32 -0.8 5,165,609 - Pf6N2E2_4433 0.32 +0.2 5,166,028 + Pf6N2E2_4433 0.66 -0.4 5,166,028 + Pf6N2E2_4433 0.66 -2.6 5,166,028 + Pf6N2E2_4433 0.66 -2.4 5,166,029 - Pf6N2E2_4433 0.66 -0.7 5,166,077 + Pf6N2E2_4433 0.70 -2.4 5,166,077 + Pf6N2E2_4433 0.70 -2.1 5,166,078 - Pf6N2E2_4433 0.70 -1.2 5,166,103 + Pf6N2E2_4433 0.72 -1.2 5,166,103 + Pf6N2E2_4433 0.72 -1.4 5,166,104 - Pf6N2E2_4433 0.72 -2.7 5,166,127 + Pf6N2E2_4433 0.74 +0.4 5,166,271 + Pf6N2E2_4433 0.85 -0.8 5,166,482 + -1.9 5,166,482 + +0.5 5,166,482 + +0.1 5,166,483 - -0.6 5,166,483 - +1.6 5,166,483 - -2.1 5,166,508 - +0.3 5,166,508 - +0.2 5,166,537 + +1.1 5,166,537 + +0.0 5,166,537 + -1.0 5,166,537 + -0.6 5,166,537 + -1.6 5,166,538 - -0.0 5,166,538 - +1.3 5,166,564 - -0.2 5,166,588 - -1.6 5,166,614 + -1.7 5,166,615 - +0.5 5,166,703 - -1.7 5,166,711 - +0.9 5,166,728 - -0.1 5,166,755 + +1.7 5,166,755 + -0.7 5,166,756 - +0.1 5,166,756 - -1.2 5,166,818 + +1.0 5,166,839 - -1.0 5,166,839 - -1.0 5,166,925 + Pf6N2E2_4434 0.14 -1.6 5,166,925 + Pf6N2E2_4434 0.14 -1.7 5,166,925 + Pf6N2E2_4434 0.14 -1.0 5,166,934 + Pf6N2E2_4434 0.15 +0.3 5,166,934 + Pf6N2E2_4434 0.15 -0.6 5,166,998 + Pf6N2E2_4434 0.27 +0.1 5,166,998 + Pf6N2E2_4434 0.27 +0.7 5,166,998 + Pf6N2E2_4434 0.27 +0.7 5,166,998 + Pf6N2E2_4434 0.27 -1.7 5,166,998 + Pf6N2E2_4434 0.27 +1.6 5,166,998 + Pf6N2E2_4434 0.27 -0.1 5,166,999 - Pf6N2E2_4434 0.27 +0.3 5,167,022 + Pf6N2E2_4434 0.32 +0.5 5,167,022 + Pf6N2E2_4434 0.32 -2.0 5,167,022 + Pf6N2E2_4434 0.32 +0.0 5,167,022 + Pf6N2E2_4434 0.32 -1.9 5,167,022 + Pf6N2E2_4434 0.32 +0.8 5,167,022 + Pf6N2E2_4434 0.32 +0.0 5,167,022 + Pf6N2E2_4434 0.32 -3.0 5,167,023 - Pf6N2E2_4434 0.32 -0.7 5,167,023 - Pf6N2E2_4434 0.32 -3.9 5,167,054 + Pf6N2E2_4434 0.38 -1.2 5,167,054 + Pf6N2E2_4434 0.38 +0.3 5,167,054 + Pf6N2E2_4434 0.38 +1.6 5,167,054 + Pf6N2E2_4434 0.38 +1.0 5,167,054 + Pf6N2E2_4434 0.38 -0.1 5,167,055 - Pf6N2E2_4434 0.38 +0.4 5,167,055 - Pf6N2E2_4434 0.38 -0.7 5,167,055 - Pf6N2E2_4434 0.38 +0.5 5,167,230 + Pf6N2E2_4434 0.70 -1.3 5,167,231 - Pf6N2E2_4434 0.70 -1.0 5,167,257 + Pf6N2E2_4434 0.75 -0.7 5,167,309 + Pf6N2E2_4434 0.85 -1.7 5,167,309 + Pf6N2E2_4434 0.85 -0.7 5,167,310 - Pf6N2E2_4434 0.85 -0.4 5,167,313 + Pf6N2E2_4434 0.85 +1.1 5,167,313 + Pf6N2E2_4434 0.85 +0.7 5,167,313 + Pf6N2E2_4434 0.85 +0.5 5,167,313 + Pf6N2E2_4434 0.85 +1.8 5,167,313 + Pf6N2E2_4434 0.85 +1.0 5,167,313 + Pf6N2E2_4434 0.85 +2.4 5,167,313 + Pf6N2E2_4434 0.85 -1.6 5,167,314 - Pf6N2E2_4434 0.85 -1.1 5,167,314 - Pf6N2E2_4434 0.85 +0.0 5,167,314 - Pf6N2E2_4434 0.85 -0.0 5,167,314 - Pf6N2E2_4434 0.85 +0.1 5,167,314 - Pf6N2E2_4434 0.85 -0.8 5,167,314 - Pf6N2E2_4434 0.85 +0.0 5,167,363 - +1.6 5,167,400 + +1.4 5,167,400 + -0.7 5,167,400 + -1.0 5,167,401 - +0.3 5,167,401 - -0.7 5,167,401 - -1.0 5,167,401 - +0.0 5,167,401 - -0.3 5,167,409 + -0.3 5,167,410 - -0.8 5,167,410 - +0.3 5,167,420 + -0.8 5,167,420 + -1.2 5,167,421 - -1.2 5,167,447 + -0.2 5,167,447 + -1.4
Or see this region's nucleotide sequence