Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_351 and Pf6N2E2_352 are separated by 203 nucleotides Pf6N2E2_352 and Pf6N2E2_353 are separated by 166 nucleotides Pf6N2E2_353 and Pf6N2E2_354 overlap by 11 nucleotides
Pf6N2E2_351 - Aldehyde dehydrogenase (EC 1.2.1.3), at 416,238 to 417,686
_351
Pf6N2E2_352 - Opine oxidase subunit B, at 417,890 to 419,071
_352
Pf6N2E2_353 - Bona fide RidA/YjgF/TdcF/RutC subgroup, at 419,238 to 419,642
_353
Pf6N2E2_354 - Type III PLP / low-specificity D-threonine aldolase, at 419,632 to 420,711
_354
Position (kb) 417
418
419
420 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 417.212 kb on - strand, within Pf6N2E2_351 at 417.301 kb on + strand, within Pf6N2E2_351 at 417.301 kb on + strand, within Pf6N2E2_351 at 417.301 kb on + strand, within Pf6N2E2_351 at 417.302 kb on - strand, within Pf6N2E2_351 at 417.302 kb on - strand, within Pf6N2E2_351 at 417.330 kb on + strand, within Pf6N2E2_351 at 417.531 kb on + strand, within Pf6N2E2_351 at 417.640 kb on + strand at 417.640 kb on + strand at 417.640 kb on + strand at 417.640 kb on + strand at 417.641 kb on - strand at 417.641 kb on - strand at 417.641 kb on - strand at 417.641 kb on - strand at 417.641 kb on - strand at 417.701 kb on + strand at 417.701 kb on + strand at 417.712 kb on + strand at 417.712 kb on + strand at 417.713 kb on - strand at 417.713 kb on - strand at 417.759 kb on + strand at 417.760 kb on - strand at 417.760 kb on - strand at 417.781 kb on + strand at 417.818 kb on + strand at 417.819 kb on - strand at 417.819 kb on - strand at 418.075 kb on - strand, within Pf6N2E2_352 at 418.075 kb on - strand, within Pf6N2E2_352 at 418.131 kb on + strand, within Pf6N2E2_352 at 418.131 kb on + strand, within Pf6N2E2_352 at 418.132 kb on - strand, within Pf6N2E2_352 at 418.132 kb on - strand, within Pf6N2E2_352 at 418.132 kb on - strand, within Pf6N2E2_352 at 418.317 kb on + strand, within Pf6N2E2_352 at 418.318 kb on - strand, within Pf6N2E2_352 at 418.318 kb on - strand, within Pf6N2E2_352 at 418.335 kb on - strand, within Pf6N2E2_352 at 418.496 kb on + strand, within Pf6N2E2_352 at 418.497 kb on - strand, within Pf6N2E2_352 at 418.653 kb on + strand, within Pf6N2E2_352 at 418.654 kb on - strand, within Pf6N2E2_352 at 418.870 kb on - strand, within Pf6N2E2_352 at 418.870 kb on - strand, within Pf6N2E2_352 at 418.948 kb on - strand, within Pf6N2E2_352 at 419.048 kb on + strand at 419.048 kb on + strand at 419.048 kb on + strand at 419.048 kb on + strand at 419.049 kb on - strand at 419.049 kb on - strand at 419.049 kb on - strand at 419.075 kb on - strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.113 kb on - strand at 419.113 kb on - strand at 419.113 kb on - strand at 419.113 kb on - strand at 419.113 kb on - strand at 419.113 kb on - strand at 419.239 kb on + strand at 419.239 kb on + strand at 419.240 kb on - strand at 419.291 kb on - strand, within Pf6N2E2_353 at 419.291 kb on - strand, within Pf6N2E2_353 at 419.351 kb on - strand, within Pf6N2E2_353 at 419.359 kb on + strand, within Pf6N2E2_353 at 419.401 kb on + strand, within Pf6N2E2_353 at 419.401 kb on + strand, within Pf6N2E2_353 at 419.401 kb on + strand, within Pf6N2E2_353 at 419.401 kb on + strand, within Pf6N2E2_353 at 419.401 kb on + strand, within Pf6N2E2_353 at 419.402 kb on - strand, within Pf6N2E2_353 at 419.402 kb on - strand, within Pf6N2E2_353 at 419.402 kb on - strand, within Pf6N2E2_353 at 419.402 kb on - strand, within Pf6N2E2_353 at 419.551 kb on + strand, within Pf6N2E2_353 at 419.551 kb on + strand, within Pf6N2E2_353 at 419.552 kb on - strand, within Pf6N2E2_353 at 419.552 kb on - strand, within Pf6N2E2_353 at 419.552 kb on - strand, within Pf6N2E2_353 at 419.552 kb on - strand, within Pf6N2E2_353 at 419.584 kb on + strand, within Pf6N2E2_353 at 419.585 kb on - strand, within Pf6N2E2_353 at 419.691 kb on - strand at 419.691 kb on - strand at 419.691 kb on - strand at 420.059 kb on + strand, within Pf6N2E2_354 at 420.059 kb on + strand, within Pf6N2E2_354 at 420.059 kb on + strand, within Pf6N2E2_354 at 420.059 kb on + strand, within Pf6N2E2_354 at 420.059 kb on + strand, within Pf6N2E2_354 at 420.060 kb on - strand, within Pf6N2E2_354 at 420.060 kb on - strand, within Pf6N2E2_354 at 420.060 kb on - strand, within Pf6N2E2_354
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 417,212 - Pf6N2E2_351 0.67 -0.4 417,301 + Pf6N2E2_351 0.73 -0.2 417,301 + Pf6N2E2_351 0.73 +1.6 417,301 + Pf6N2E2_351 0.73 -0.7 417,302 - Pf6N2E2_351 0.73 +0.6 417,302 - Pf6N2E2_351 0.73 +2.9 417,330 + Pf6N2E2_351 0.75 -0.4 417,531 + Pf6N2E2_351 0.89 +0.6 417,640 + +0.6 417,640 + -0.4 417,640 + +0.7 417,640 + +0.3 417,641 - +1.2 417,641 - -1.7 417,641 - -0.4 417,641 - -0.3 417,641 - +0.1 417,701 + -1.3 417,701 + -0.3 417,712 + -2.9 417,712 + +0.2 417,713 - -0.6 417,713 - +0.2 417,759 + +0.9 417,760 - -0.4 417,760 - +2.0 417,781 + +1.2 417,818 + -1.9 417,819 - -0.5 417,819 - +0.5 418,075 - Pf6N2E2_352 0.16 +2.3 418,075 - Pf6N2E2_352 0.16 +0.7 418,131 + Pf6N2E2_352 0.20 -0.2 418,131 + Pf6N2E2_352 0.20 +1.4 418,132 - Pf6N2E2_352 0.20 +0.7 418,132 - Pf6N2E2_352 0.20 -2.4 418,132 - Pf6N2E2_352 0.20 -0.7 418,317 + Pf6N2E2_352 0.36 -0.9 418,318 - Pf6N2E2_352 0.36 -0.3 418,318 - Pf6N2E2_352 0.36 -0.2 418,335 - Pf6N2E2_352 0.38 +0.9 418,496 + Pf6N2E2_352 0.51 +0.0 418,497 - Pf6N2E2_352 0.51 +1.2 418,653 + Pf6N2E2_352 0.65 -1.0 418,654 - Pf6N2E2_352 0.65 +0.0 418,870 - Pf6N2E2_352 0.83 +0.7 418,870 - Pf6N2E2_352 0.83 -1.5 418,948 - Pf6N2E2_352 0.90 +0.2 419,048 + -1.1 419,048 + +1.9 419,048 + -0.5 419,048 + -0.5 419,049 - +0.7 419,049 - +1.3 419,049 - -3.1 419,075 - -0.2 419,112 + -0.3 419,112 + -2.6 419,112 + +0.2 419,112 + -1.7 419,112 + -1.0 419,112 + +1.4 419,112 + +0.4 419,112 + -0.4 419,112 + +0.7 419,113 - -1.2 419,113 - +0.6 419,113 - -1.7 419,113 - -1.0 419,113 - -1.7 419,113 - +2.5 419,239 + -0.4 419,239 + +0.8 419,240 - +1.4 419,291 - Pf6N2E2_353 0.13 -0.3 419,291 - Pf6N2E2_353 0.13 +1.5 419,351 - Pf6N2E2_353 0.28 +0.5 419,359 + Pf6N2E2_353 0.30 -0.2 419,401 + Pf6N2E2_353 0.40 -0.3 419,401 + Pf6N2E2_353 0.40 +1.6 419,401 + Pf6N2E2_353 0.40 -0.6 419,401 + Pf6N2E2_353 0.40 -0.5 419,401 + Pf6N2E2_353 0.40 +0.1 419,402 - Pf6N2E2_353 0.40 -0.5 419,402 - Pf6N2E2_353 0.40 -1.0 419,402 - Pf6N2E2_353 0.40 +1.4 419,402 - Pf6N2E2_353 0.40 -0.7 419,551 + Pf6N2E2_353 0.77 +1.0 419,551 + Pf6N2E2_353 0.77 -0.2 419,552 - Pf6N2E2_353 0.78 +1.1 419,552 - Pf6N2E2_353 0.78 -1.4 419,552 - Pf6N2E2_353 0.78 -0.4 419,552 - Pf6N2E2_353 0.78 -3.0 419,584 + Pf6N2E2_353 0.85 -2.7 419,585 - Pf6N2E2_353 0.86 +0.2 419,691 - -0.1 419,691 - -2.0 419,691 - -1.3 420,059 + Pf6N2E2_354 0.40 +0.3 420,059 + Pf6N2E2_354 0.40 -2.4 420,059 + Pf6N2E2_354 0.40 -1.5 420,059 + Pf6N2E2_354 0.40 -1.9 420,059 + Pf6N2E2_354 0.40 -1.0 420,060 - Pf6N2E2_354 0.40 +1.2 420,060 - Pf6N2E2_354 0.40 -1.7 420,060 - Pf6N2E2_354 0.40 -1.0
Or see this region's nucleotide sequence