Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3373 and Pf6N2E2_3374 are separated by 59 nucleotides Pf6N2E2_3374 and Pf6N2E2_3375 are separated by 11 nucleotides Pf6N2E2_3375 and Pf6N2E2_3376 are separated by 7 nucleotides Pf6N2E2_3376 and Pf6N2E2_3377 are separated by 84 nucleotides Pf6N2E2_3377 and Pf6N2E2_3378 are separated by 10 nucleotides Pf6N2E2_3378 and Pf6N2E2_3379 are separated by 195 nucleotides
Pf6N2E2_3373 - Outer membrane lipoprotein SmpA, a component of the essential YaeT outer-membrane protein assembly complex, at 3,905,971 to 3,906,324
_3373
Pf6N2E2_3374 - UPF0125 protein yfjF, at 3,906,384 to 3,906,671
_3374
Pf6N2E2_3375 - Putative oligoketide cyclase/lipid transport protein, similarity with yeast ubiquinone-binding protein YOL008W, at 3,906,683 to 3,907,126
_3375
Pf6N2E2_3376 - Sodium-dependent transporter family protein, at 3,907,134 to 3,908,426
_3376
Pf6N2E2_3377 - hypothetical protein, at 3,908,511 to 3,908,645
_3377
Pf6N2E2_3378 - tmRNA-binding protein SmpB, at 3,908,656 to 3,909,138
_3378
Pf6N2E2_3379 - Lactate-responsive regulator LldR in Enterobacteria, GntR family, at 3,909,334 to 3,910,101
_3379
Position (kb)
3907
3908
3909 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3906.282 kb on - strand, within Pf6N2E2_3373 at 3906.310 kb on - strand at 3906.310 kb on - strand at 3906.322 kb on + strand at 3906.322 kb on + strand at 3906.322 kb on + strand at 3906.322 kb on + strand at 3906.323 kb on - strand at 3906.323 kb on - strand at 3906.323 kb on - strand at 3906.362 kb on - strand at 3906.362 kb on - strand at 3906.385 kb on + strand at 3906.676 kb on - strand at 3906.676 kb on - strand at 3907.129 kb on - strand at 3907.129 kb on - strand at 3907.129 kb on - strand at 3907.129 kb on - strand at 3907.129 kb on - strand at 3907.129 kb on - strand at 3907.129 kb on - strand at 3907.129 kb on - strand at 3907.129 kb on - strand at 3907.162 kb on - strand at 3907.162 kb on - strand at 3907.162 kb on - strand at 3907.162 kb on - strand at 3907.187 kb on - strand at 3907.187 kb on - strand at 3907.187 kb on - strand at 3907.187 kb on - strand at 3907.187 kb on - strand at 3907.187 kb on - strand at 3907.219 kb on - strand at 3907.379 kb on - strand, within Pf6N2E2_3376 at 3907.429 kb on - strand, within Pf6N2E2_3376 at 3907.429 kb on - strand, within Pf6N2E2_3376 at 3907.429 kb on - strand, within Pf6N2E2_3376 at 3907.429 kb on - strand, within Pf6N2E2_3376 at 3907.429 kb on - strand, within Pf6N2E2_3376 at 3907.429 kb on - strand, within Pf6N2E2_3376 at 3907.429 kb on - strand, within Pf6N2E2_3376 at 3907.429 kb on - strand, within Pf6N2E2_3376 at 3907.429 kb on - strand, within Pf6N2E2_3376 at 3907.429 kb on - strand, within Pf6N2E2_3376 at 3907.429 kb on - strand, within Pf6N2E2_3376 at 3907.429 kb on - strand, within Pf6N2E2_3376 at 3907.429 kb on - strand, within Pf6N2E2_3376 at 3907.431 kb on - strand, within Pf6N2E2_3376 at 3907.431 kb on - strand, within Pf6N2E2_3376 at 3907.431 kb on - strand, within Pf6N2E2_3376 at 3907.431 kb on - strand, within Pf6N2E2_3376 at 3907.431 kb on - strand, within Pf6N2E2_3376 at 3907.431 kb on - strand, within Pf6N2E2_3376 at 3907.431 kb on - strand, within Pf6N2E2_3376 at 3907.431 kb on - strand, within Pf6N2E2_3376 at 3907.431 kb on - strand, within Pf6N2E2_3376 at 3907.431 kb on - strand, within Pf6N2E2_3376 at 3907.431 kb on - strand, within Pf6N2E2_3376 at 3907.431 kb on - strand, within Pf6N2E2_3376 at 3907.431 kb on - strand, within Pf6N2E2_3376 at 3907.529 kb on - strand at 3907.529 kb on - strand, within Pf6N2E2_3376 at 3907.529 kb on - strand, within Pf6N2E2_3376 at 3907.799 kb on - strand, within Pf6N2E2_3376 at 3907.799 kb on - strand, within Pf6N2E2_3376 at 3907.799 kb on - strand, within Pf6N2E2_3376 at 3907.799 kb on - strand, within Pf6N2E2_3376 at 3907.799 kb on - strand, within Pf6N2E2_3376 at 3907.799 kb on - strand, within Pf6N2E2_3376 at 3907.799 kb on - strand, within Pf6N2E2_3376 at 3907.808 kb on - strand, within Pf6N2E2_3376 at 3907.808 kb on - strand, within Pf6N2E2_3376 at 3907.808 kb on - strand, within Pf6N2E2_3376 at 3907.808 kb on - strand, within Pf6N2E2_3376 at 3908.174 kb on - strand, within Pf6N2E2_3376 at 3908.174 kb on - strand, within Pf6N2E2_3376 at 3908.174 kb on - strand, within Pf6N2E2_3376 at 3908.174 kb on - strand, within Pf6N2E2_3376 at 3908.202 kb on - strand, within Pf6N2E2_3376 at 3908.202 kb on - strand, within Pf6N2E2_3376 at 3908.203 kb on + strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.290 kb on - strand, within Pf6N2E2_3376 at 3908.429 kb on - strand at 3908.429 kb on - strand at 3908.429 kb on - strand at 3908.445 kb on - strand at 3908.445 kb on - strand at 3908.445 kb on - strand at 3908.445 kb on - strand at 3908.445 kb on - strand at 3908.482 kb on - strand at 3908.482 kb on - strand at 3908.482 kb on - strand at 3908.641 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.411 kb on - strand, within Pf6N2E2_3379 at 3909.411 kb on - strand, within Pf6N2E2_3379 at 3909.411 kb on - strand, within Pf6N2E2_3379 at 3909.411 kb on - strand, within Pf6N2E2_3379 at 3909.411 kb on - strand, within Pf6N2E2_3379 at 3909.411 kb on - strand, within Pf6N2E2_3379 at 3909.417 kb on + strand, within Pf6N2E2_3379 at 3909.417 kb on + strand, within Pf6N2E2_3379 at 3909.417 kb on + strand, within Pf6N2E2_3379 at 3909.417 kb on + strand, within Pf6N2E2_3379 at 3909.417 kb on + strand, within Pf6N2E2_3379 at 3909.417 kb on + strand, within Pf6N2E2_3379 at 3909.417 kb on + strand, within Pf6N2E2_3379 at 3909.417 kb on + strand, within Pf6N2E2_3379 at 3909.418 kb on - strand, within Pf6N2E2_3379 at 3909.418 kb on - strand, within Pf6N2E2_3379 at 3909.418 kb on - strand, within Pf6N2E2_3379 at 3909.418 kb on - strand, within Pf6N2E2_3379 at 3909.418 kb on - strand, within Pf6N2E2_3379 at 3909.418 kb on - strand, within Pf6N2E2_3379
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 3,906,282 - Pf6N2E2_3373 0.88 -0.1 3,906,310 - -1.5 3,906,310 - -0.0 3,906,322 + -0.1 3,906,322 + +0.6 3,906,322 + +0.5 3,906,322 + -1.1 3,906,323 - -1.7 3,906,323 - +0.8 3,906,323 - +0.6 3,906,362 - -0.6 3,906,362 - +1.0 3,906,385 + +1.3 3,906,676 - -1.7 3,906,676 - -1.1 3,907,129 - +1.1 3,907,129 - -2.1 3,907,129 - +2.3 3,907,129 - +0.7 3,907,129 - -0.7 3,907,129 - +0.2 3,907,129 - -0.5 3,907,129 - +0.7 3,907,129 - -0.7 3,907,162 - +1.5 3,907,162 - +1.3 3,907,162 - -0.1 3,907,162 - +1.8 3,907,187 - +0.9 3,907,187 - -0.2 3,907,187 - -0.9 3,907,187 - +1.9 3,907,187 - +1.8 3,907,187 - -2.3 3,907,219 - +2.2 3,907,379 - Pf6N2E2_3376 0.19 -0.8 3,907,429 - Pf6N2E2_3376 0.23 +0.9 3,907,429 - Pf6N2E2_3376 0.23 +2.7 3,907,429 - Pf6N2E2_3376 0.23 -0.3 3,907,429 - Pf6N2E2_3376 0.23 +1.0 3,907,429 - Pf6N2E2_3376 0.23 +1.5 3,907,429 - Pf6N2E2_3376 0.23 +1.3 3,907,429 - Pf6N2E2_3376 0.23 +1.2 3,907,429 - Pf6N2E2_3376 0.23 +1.1 3,907,429 - Pf6N2E2_3376 0.23 +2.5 3,907,429 - Pf6N2E2_3376 0.23 -2.5 3,907,429 - Pf6N2E2_3376 0.23 -2.2 3,907,429 - Pf6N2E2_3376 0.23 -0.7 3,907,429 - Pf6N2E2_3376 0.23 +2.1 3,907,431 - Pf6N2E2_3376 0.23 +0.6 3,907,431 - Pf6N2E2_3376 0.23 +2.6 3,907,431 - Pf6N2E2_3376 0.23 +2.1 3,907,431 - Pf6N2E2_3376 0.23 +0.3 3,907,431 - Pf6N2E2_3376 0.23 +2.3 3,907,431 - Pf6N2E2_3376 0.23 +1.4 3,907,431 - Pf6N2E2_3376 0.23 -1.9 3,907,431 - Pf6N2E2_3376 0.23 +1.6 3,907,431 - Pf6N2E2_3376 0.23 +0.3 3,907,431 - Pf6N2E2_3376 0.23 -1.5 3,907,431 - Pf6N2E2_3376 0.23 +2.7 3,907,431 - Pf6N2E2_3376 0.23 +2.4 3,907,431 - Pf6N2E2_3376 0.23 -0.7 3,907,529 - +0.3 3,907,529 - Pf6N2E2_3376 0.31 +2.0 3,907,529 - Pf6N2E2_3376 0.31 +3.9 3,907,799 - Pf6N2E2_3376 0.51 +3.0 3,907,799 - Pf6N2E2_3376 0.51 -0.1 3,907,799 - Pf6N2E2_3376 0.51 +0.6 3,907,799 - Pf6N2E2_3376 0.51 +0.3 3,907,799 - Pf6N2E2_3376 0.51 -0.3 3,907,799 - Pf6N2E2_3376 0.51 -0.5 3,907,799 - Pf6N2E2_3376 0.51 -1.1 3,907,808 - Pf6N2E2_3376 0.52 +1.3 3,907,808 - Pf6N2E2_3376 0.52 +0.4 3,907,808 - Pf6N2E2_3376 0.52 +1.9 3,907,808 - Pf6N2E2_3376 0.52 +0.9 3,908,174 - Pf6N2E2_3376 0.80 -0.6 3,908,174 - Pf6N2E2_3376 0.80 +0.5 3,908,174 - Pf6N2E2_3376 0.80 -1.5 3,908,174 - Pf6N2E2_3376 0.80 +2.3 3,908,202 - Pf6N2E2_3376 0.83 -0.7 3,908,202 - Pf6N2E2_3376 0.83 -0.9 3,908,203 + Pf6N2E2_3376 0.83 +1.3 3,908,204 - Pf6N2E2_3376 0.83 +0.9 3,908,204 - Pf6N2E2_3376 0.83 +1.7 3,908,204 - Pf6N2E2_3376 0.83 +1.3 3,908,204 - Pf6N2E2_3376 0.83 +1.8 3,908,204 - Pf6N2E2_3376 0.83 +2.1 3,908,204 - Pf6N2E2_3376 0.83 +0.5 3,908,204 - Pf6N2E2_3376 0.83 -1.9 3,908,204 - Pf6N2E2_3376 0.83 +0.6 3,908,204 - Pf6N2E2_3376 0.83 -0.0 3,908,204 - Pf6N2E2_3376 0.83 -0.4 3,908,204 - Pf6N2E2_3376 0.83 +0.7 3,908,204 - Pf6N2E2_3376 0.83 +0.8 3,908,290 - Pf6N2E2_3376 0.89 +1.3 3,908,429 - +1.1 3,908,429 - -0.7 3,908,429 - -0.3 3,908,445 - +0.4 3,908,445 - +0.7 3,908,445 - +1.7 3,908,445 - +1.4 3,908,445 - +0.5 3,908,482 - -1.5 3,908,482 - +1.9 3,908,482 - -3.1 3,908,641 + -0.5 3,909,410 + +0.0 3,909,410 + -1.1 3,909,410 + +1.3 3,909,410 + -1.1 3,909,410 + +1.2 3,909,410 + +4.0 3,909,410 + -0.0 3,909,410 + +1.2 3,909,410 + +1.5 3,909,410 + +2.7 3,909,411 - Pf6N2E2_3379 0.10 +2.4 3,909,411 - Pf6N2E2_3379 0.10 -0.7 3,909,411 - Pf6N2E2_3379 0.10 +3.6 3,909,411 - Pf6N2E2_3379 0.10 +2.7 3,909,411 - Pf6N2E2_3379 0.10 -0.3 3,909,411 - Pf6N2E2_3379 0.10 -0.3 3,909,417 + Pf6N2E2_3379 0.11 +1.0 3,909,417 + Pf6N2E2_3379 0.11 -1.9 3,909,417 + Pf6N2E2_3379 0.11 +0.1 3,909,417 + Pf6N2E2_3379 0.11 -0.7 3,909,417 + Pf6N2E2_3379 0.11 +1.3 3,909,417 + Pf6N2E2_3379 0.11 -3.1 3,909,417 + Pf6N2E2_3379 0.11 +0.5 3,909,417 + Pf6N2E2_3379 0.11 +3.4 3,909,418 - Pf6N2E2_3379 0.11 -0.4 3,909,418 - Pf6N2E2_3379 0.11 +2.6 3,909,418 - Pf6N2E2_3379 0.11 +1.6 3,909,418 - Pf6N2E2_3379 0.11 -0.3 3,909,418 - Pf6N2E2_3379 0.11 +0.3 3,909,418 - Pf6N2E2_3379 0.11 -2.2
Or see this region's nucleotide sequence