Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2974

Experiment: RCH2 defined with Cobalt chloride 0.04 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2973 and Pf6N2E2_2974 are separated by 214 nucleotidesPf6N2E2_2974 and Pf6N2E2_6114 are separated by 174 nucleotidesPf6N2E2_6114 and Pf6N2E2_6115 are separated by 223 nucleotidesPf6N2E2_6115 and Pf6N2E2_2975 are separated by 156 nucleotides Pf6N2E2_2973 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 3,464,272 to 3,465,225 _2973 Pf6N2E2_2974 - DNA polymerase IV (EC 2.7.7.7), at 3,465,440 to 3,466,612 _2974 Pf6N2E2_6114 - tRNA-Met-CAT, at 3,466,787 to 3,466,860 _6114 Pf6N2E2_6115 - tRNA-Met-CAT, at 3,467,084 to 3,467,157 _6115 Pf6N2E2_2975 - membrane protein, putative, at 3,467,314 to 3,469,956 _2975 Position (kb) 3465 3466 3467Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3464.515 kb on + strand, within Pf6N2E2_2973at 3464.525 kb on - strand, within Pf6N2E2_2973at 3464.555 kb on - strand, within Pf6N2E2_2973at 3464.665 kb on + strand, within Pf6N2E2_2973at 3464.665 kb on + strand, within Pf6N2E2_2973at 3464.665 kb on + strand, within Pf6N2E2_2973at 3464.665 kb on + strand, within Pf6N2E2_2973at 3464.666 kb on - strand, within Pf6N2E2_2973at 3464.666 kb on - strand, within Pf6N2E2_2973at 3464.666 kb on - strand, within Pf6N2E2_2973at 3464.666 kb on - strand, within Pf6N2E2_2973at 3465.023 kb on - strand, within Pf6N2E2_2973at 3465.023 kb on - strand, within Pf6N2E2_2973at 3465.314 kb on + strandat 3465.315 kb on - strandat 3465.315 kb on - strandat 3465.315 kb on - strandat 3465.316 kb on + strandat 3465.316 kb on + strandat 3465.316 kb on + strandat 3465.316 kb on + strandat 3465.317 kb on - strandat 3465.317 kb on - strandat 3465.317 kb on - strandat 3465.561 kb on + strand, within Pf6N2E2_2974at 3465.561 kb on + strand, within Pf6N2E2_2974at 3465.562 kb on - strand, within Pf6N2E2_2974at 3465.562 kb on - strand, within Pf6N2E2_2974at 3465.750 kb on + strand, within Pf6N2E2_2974at 3465.750 kb on + strand, within Pf6N2E2_2974at 3465.751 kb on - strand, within Pf6N2E2_2974at 3465.751 kb on - strand, within Pf6N2E2_2974at 3465.751 kb on - strand, within Pf6N2E2_2974at 3465.751 kb on - strand, within Pf6N2E2_2974at 3465.751 kb on - strand, within Pf6N2E2_2974at 3466.180 kb on - strand, within Pf6N2E2_2974at 3466.218 kb on + strand, within Pf6N2E2_2974at 3466.219 kb on - strand, within Pf6N2E2_2974at 3466.219 kb on - strand, within Pf6N2E2_2974at 3466.261 kb on - strand, within Pf6N2E2_2974at 3466.345 kb on - strand, within Pf6N2E2_2974at 3466.359 kb on + strand, within Pf6N2E2_2974at 3466.360 kb on - strand, within Pf6N2E2_2974at 3466.363 kb on + strand, within Pf6N2E2_2974at 3466.363 kb on + strand, within Pf6N2E2_2974at 3466.489 kb on + strand, within Pf6N2E2_2974at 3466.489 kb on + strand, within Pf6N2E2_2974at 3466.489 kb on + strand, within Pf6N2E2_2974at 3466.490 kb on - strand, within Pf6N2E2_2974at 3466.490 kb on - strand, within Pf6N2E2_2974at 3466.490 kb on - strand, within Pf6N2E2_2974at 3466.501 kb on + strandat 3466.501 kb on + strandat 3466.505 kb on + strandat 3466.505 kb on + strandat 3466.505 kb on + strandat 3466.505 kb on + strandat 3466.506 kb on - strandat 3466.506 kb on - strandat 3466.518 kb on + strandat 3466.519 kb on - strandat 3466.519 kb on - strandat 3466.519 kb on - strandat 3466.541 kb on + strandat 3466.558 kb on + strandat 3466.558 kb on + strandat 3466.559 kb on - strandat 3466.559 kb on - strandat 3466.559 kb on - strandat 3466.559 kb on - strandat 3466.559 kb on - strandat 3466.560 kb on + strandat 3466.560 kb on + strandat 3466.560 kb on + strandat 3466.560 kb on + strandat 3466.562 kb on + strandat 3466.959 kb on + strandat 3466.989 kb on + strandat 3466.989 kb on + strandat 3466.989 kb on + strandat 3467.018 kb on - strandat 3467.030 kb on + strandat 3467.163 kb on + strandat 3467.163 kb on + strandat 3467.163 kb on + strandat 3467.163 kb on + strandat 3467.224 kb on + strandat 3467.225 kb on - strandat 3467.242 kb on - strandat 3467.264 kb on - strandat 3467.264 kb on - strandat 3467.264 kb on - strandat 3467.296 kb on + strandat 3467.296 kb on + strandat 3467.350 kb on - strandat 3467.350 kb on - strandat 3467.418 kb on + strandat 3467.419 kb on - strandat 3467.419 kb on - strandat 3467.419 kb on - strandat 3467.419 kb on - strandat 3467.441 kb on + strandat 3467.441 kb on + strandat 3467.590 kb on + strand, within Pf6N2E2_2975

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM
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3,464,515 + Pf6N2E2_2973 0.25 -1.5
3,464,525 - Pf6N2E2_2973 0.27 +0.0
3,464,555 - Pf6N2E2_2973 0.30 +1.1
3,464,665 + Pf6N2E2_2973 0.41 -0.0
3,464,665 + Pf6N2E2_2973 0.41 -0.2
3,464,665 + Pf6N2E2_2973 0.41 +0.7
3,464,665 + Pf6N2E2_2973 0.41 +0.6
3,464,666 - Pf6N2E2_2973 0.41 +0.8
3,464,666 - Pf6N2E2_2973 0.41 -0.6
3,464,666 - Pf6N2E2_2973 0.41 +0.2
3,464,666 - Pf6N2E2_2973 0.41 +1.1
3,465,023 - Pf6N2E2_2973 0.79 -2.2
3,465,023 - Pf6N2E2_2973 0.79 -1.5
3,465,314 + -0.1
3,465,315 - +2.0
3,465,315 - +0.2
3,465,315 - -3.0
3,465,316 + -0.6
3,465,316 + +1.1
3,465,316 + -0.0
3,465,316 + +1.3
3,465,317 - +0.5
3,465,317 - +1.5
3,465,317 - -0.5
3,465,561 + Pf6N2E2_2974 0.10 -0.3
3,465,561 + Pf6N2E2_2974 0.10 -0.2
3,465,562 - Pf6N2E2_2974 0.10 +1.2
3,465,562 - Pf6N2E2_2974 0.10 +1.0
3,465,750 + Pf6N2E2_2974 0.26 -2.5
3,465,750 + Pf6N2E2_2974 0.26 +0.8
3,465,751 - Pf6N2E2_2974 0.27 +0.4
3,465,751 - Pf6N2E2_2974 0.27 +1.2
3,465,751 - Pf6N2E2_2974 0.27 -0.7
3,465,751 - Pf6N2E2_2974 0.27 +0.3
3,465,751 - Pf6N2E2_2974 0.27 +1.0
3,466,180 - Pf6N2E2_2974 0.63 +0.6
3,466,218 + Pf6N2E2_2974 0.66 +1.6
3,466,219 - Pf6N2E2_2974 0.66 +2.6
3,466,219 - Pf6N2E2_2974 0.66 -2.2
3,466,261 - Pf6N2E2_2974 0.70 +2.3
3,466,345 - Pf6N2E2_2974 0.77 -0.6
3,466,359 + Pf6N2E2_2974 0.78 +1.2
3,466,360 - Pf6N2E2_2974 0.78 -1.3
3,466,363 + Pf6N2E2_2974 0.79 -0.0
3,466,363 + Pf6N2E2_2974 0.79 -0.6
3,466,489 + Pf6N2E2_2974 0.89 +0.9
3,466,489 + Pf6N2E2_2974 0.89 -0.2
3,466,489 + Pf6N2E2_2974 0.89 -0.0
3,466,490 - Pf6N2E2_2974 0.90 +1.3
3,466,490 - Pf6N2E2_2974 0.90 -0.3
3,466,490 - Pf6N2E2_2974 0.90 +0.5
3,466,501 + -0.2
3,466,501 + -0.0
3,466,505 + -0.4
3,466,505 + -1.6
3,466,505 + +0.5
3,466,505 + +1.3
3,466,506 - -1.0
3,466,506 - -1.9
3,466,518 + -0.0
3,466,519 - -0.6
3,466,519 - -1.3
3,466,519 - +1.0
3,466,541 + +0.9
3,466,558 + -2.2
3,466,558 + -1.0
3,466,559 - -2.3
3,466,559 - +0.4
3,466,559 - +0.4
3,466,559 - -0.1
3,466,559 - -0.2
3,466,560 + +1.0
3,466,560 + +0.3
3,466,560 + -1.8
3,466,560 + -0.5
3,466,562 + -2.3
3,466,959 + -2.7
3,466,989 + -1.5
3,466,989 + -1.8
3,466,989 + -0.2
3,467,018 - +0.5
3,467,030 + -1.1
3,467,163 + -0.1
3,467,163 + +1.5
3,467,163 + +1.5
3,467,163 + +2.0
3,467,224 + +1.0
3,467,225 - -1.5
3,467,242 - +1.1
3,467,264 - +0.8
3,467,264 - -0.2
3,467,264 - -1.0
3,467,296 + -0.0
3,467,296 + -0.6
3,467,350 - -2.6
3,467,350 - -0.5
3,467,418 + +0.8
3,467,419 - +1.0
3,467,419 - +0.7
3,467,419 - -1.0
3,467,419 - -1.6
3,467,441 + -0.4
3,467,441 + +2.0
3,467,590 + Pf6N2E2_2975 0.10 -0.6

Or see this region's nucleotide sequence