Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2973 and Pf6N2E2_2974 are separated by 214 nucleotides Pf6N2E2_2974 and Pf6N2E2_6114 are separated by 174 nucleotides Pf6N2E2_6114 and Pf6N2E2_6115 are separated by 223 nucleotides Pf6N2E2_6115 and Pf6N2E2_2975 are separated by 156 nucleotides
Pf6N2E2_2973 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 3,464,272 to 3,465,225
_2973
Pf6N2E2_2974 - DNA polymerase IV (EC 2.7.7.7), at 3,465,440 to 3,466,612
_2974
Pf6N2E2_6114 - tRNA-Met-CAT, at 3,466,787 to 3,466,860
_6114
Pf6N2E2_6115 - tRNA-Met-CAT, at 3,467,084 to 3,467,157
_6115
Pf6N2E2_2975 - membrane protein, putative, at 3,467,314 to 3,469,956
_2975
Position (kb)
3465
3466
3467 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3464.515 kb on + strand, within Pf6N2E2_2973 at 3464.525 kb on - strand, within Pf6N2E2_2973 at 3464.555 kb on - strand, within Pf6N2E2_2973 at 3464.665 kb on + strand, within Pf6N2E2_2973 at 3464.665 kb on + strand, within Pf6N2E2_2973 at 3464.665 kb on + strand, within Pf6N2E2_2973 at 3464.665 kb on + strand, within Pf6N2E2_2973 at 3464.666 kb on - strand, within Pf6N2E2_2973 at 3464.666 kb on - strand, within Pf6N2E2_2973 at 3464.666 kb on - strand, within Pf6N2E2_2973 at 3464.666 kb on - strand, within Pf6N2E2_2973 at 3465.023 kb on - strand, within Pf6N2E2_2973 at 3465.023 kb on - strand, within Pf6N2E2_2973 at 3465.314 kb on + strand at 3465.315 kb on - strand at 3465.315 kb on - strand at 3465.315 kb on - strand at 3465.316 kb on + strand at 3465.316 kb on + strand at 3465.316 kb on + strand at 3465.316 kb on + strand at 3465.317 kb on - strand at 3465.317 kb on - strand at 3465.317 kb on - strand at 3465.561 kb on + strand, within Pf6N2E2_2974 at 3465.561 kb on + strand, within Pf6N2E2_2974 at 3465.562 kb on - strand, within Pf6N2E2_2974 at 3465.562 kb on - strand, within Pf6N2E2_2974 at 3465.750 kb on + strand, within Pf6N2E2_2974 at 3465.750 kb on + strand, within Pf6N2E2_2974 at 3465.751 kb on - strand, within Pf6N2E2_2974 at 3465.751 kb on - strand, within Pf6N2E2_2974 at 3465.751 kb on - strand, within Pf6N2E2_2974 at 3465.751 kb on - strand, within Pf6N2E2_2974 at 3465.751 kb on - strand, within Pf6N2E2_2974 at 3466.180 kb on - strand, within Pf6N2E2_2974 at 3466.218 kb on + strand, within Pf6N2E2_2974 at 3466.219 kb on - strand, within Pf6N2E2_2974 at 3466.219 kb on - strand, within Pf6N2E2_2974 at 3466.261 kb on - strand, within Pf6N2E2_2974 at 3466.345 kb on - strand, within Pf6N2E2_2974 at 3466.359 kb on + strand, within Pf6N2E2_2974 at 3466.360 kb on - strand, within Pf6N2E2_2974 at 3466.363 kb on + strand, within Pf6N2E2_2974 at 3466.363 kb on + strand, within Pf6N2E2_2974 at 3466.489 kb on + strand, within Pf6N2E2_2974 at 3466.489 kb on + strand, within Pf6N2E2_2974 at 3466.489 kb on + strand, within Pf6N2E2_2974 at 3466.490 kb on - strand, within Pf6N2E2_2974 at 3466.490 kb on - strand, within Pf6N2E2_2974 at 3466.490 kb on - strand, within Pf6N2E2_2974 at 3466.501 kb on + strand at 3466.501 kb on + strand at 3466.505 kb on + strand at 3466.505 kb on + strand at 3466.505 kb on + strand at 3466.505 kb on + strand at 3466.506 kb on - strand at 3466.506 kb on - strand at 3466.518 kb on + strand at 3466.519 kb on - strand at 3466.519 kb on - strand at 3466.519 kb on - strand at 3466.541 kb on + strand at 3466.558 kb on + strand at 3466.558 kb on + strand at 3466.559 kb on - strand at 3466.559 kb on - strand at 3466.559 kb on - strand at 3466.559 kb on - strand at 3466.559 kb on - strand at 3466.560 kb on + strand at 3466.560 kb on + strand at 3466.560 kb on + strand at 3466.560 kb on + strand at 3466.562 kb on + strand at 3466.959 kb on + strand at 3466.989 kb on + strand at 3466.989 kb on + strand at 3466.989 kb on + strand at 3467.018 kb on - strand at 3467.030 kb on + strand at 3467.163 kb on + strand at 3467.163 kb on + strand at 3467.163 kb on + strand at 3467.163 kb on + strand at 3467.224 kb on + strand at 3467.225 kb on - strand at 3467.242 kb on - strand at 3467.264 kb on - strand at 3467.264 kb on - strand at 3467.264 kb on - strand at 3467.296 kb on + strand at 3467.296 kb on + strand at 3467.350 kb on - strand at 3467.350 kb on - strand at 3467.418 kb on + strand at 3467.419 kb on - strand at 3467.419 kb on - strand at 3467.419 kb on - strand at 3467.419 kb on - strand at 3467.441 kb on + strand at 3467.441 kb on + strand at 3467.590 kb on + strand, within Pf6N2E2_2975
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 3,464,515 + Pf6N2E2_2973 0.25 -1.5 3,464,525 - Pf6N2E2_2973 0.27 +0.0 3,464,555 - Pf6N2E2_2973 0.30 +1.1 3,464,665 + Pf6N2E2_2973 0.41 -0.0 3,464,665 + Pf6N2E2_2973 0.41 -0.2 3,464,665 + Pf6N2E2_2973 0.41 +0.7 3,464,665 + Pf6N2E2_2973 0.41 +0.6 3,464,666 - Pf6N2E2_2973 0.41 +0.8 3,464,666 - Pf6N2E2_2973 0.41 -0.6 3,464,666 - Pf6N2E2_2973 0.41 +0.2 3,464,666 - Pf6N2E2_2973 0.41 +1.1 3,465,023 - Pf6N2E2_2973 0.79 -2.2 3,465,023 - Pf6N2E2_2973 0.79 -1.5 3,465,314 + -0.1 3,465,315 - +2.0 3,465,315 - +0.2 3,465,315 - -3.0 3,465,316 + -0.6 3,465,316 + +1.1 3,465,316 + -0.0 3,465,316 + +1.3 3,465,317 - +0.5 3,465,317 - +1.5 3,465,317 - -0.5 3,465,561 + Pf6N2E2_2974 0.10 -0.3 3,465,561 + Pf6N2E2_2974 0.10 -0.2 3,465,562 - Pf6N2E2_2974 0.10 +1.2 3,465,562 - Pf6N2E2_2974 0.10 +1.0 3,465,750 + Pf6N2E2_2974 0.26 -2.5 3,465,750 + Pf6N2E2_2974 0.26 +0.8 3,465,751 - Pf6N2E2_2974 0.27 +0.4 3,465,751 - Pf6N2E2_2974 0.27 +1.2 3,465,751 - Pf6N2E2_2974 0.27 -0.7 3,465,751 - Pf6N2E2_2974 0.27 +0.3 3,465,751 - Pf6N2E2_2974 0.27 +1.0 3,466,180 - Pf6N2E2_2974 0.63 +0.6 3,466,218 + Pf6N2E2_2974 0.66 +1.6 3,466,219 - Pf6N2E2_2974 0.66 +2.6 3,466,219 - Pf6N2E2_2974 0.66 -2.2 3,466,261 - Pf6N2E2_2974 0.70 +2.3 3,466,345 - Pf6N2E2_2974 0.77 -0.6 3,466,359 + Pf6N2E2_2974 0.78 +1.2 3,466,360 - Pf6N2E2_2974 0.78 -1.3 3,466,363 + Pf6N2E2_2974 0.79 -0.0 3,466,363 + Pf6N2E2_2974 0.79 -0.6 3,466,489 + Pf6N2E2_2974 0.89 +0.9 3,466,489 + Pf6N2E2_2974 0.89 -0.2 3,466,489 + Pf6N2E2_2974 0.89 -0.0 3,466,490 - Pf6N2E2_2974 0.90 +1.3 3,466,490 - Pf6N2E2_2974 0.90 -0.3 3,466,490 - Pf6N2E2_2974 0.90 +0.5 3,466,501 + -0.2 3,466,501 + -0.0 3,466,505 + -0.4 3,466,505 + -1.6 3,466,505 + +0.5 3,466,505 + +1.3 3,466,506 - -1.0 3,466,506 - -1.9 3,466,518 + -0.0 3,466,519 - -0.6 3,466,519 - -1.3 3,466,519 - +1.0 3,466,541 + +0.9 3,466,558 + -2.2 3,466,558 + -1.0 3,466,559 - -2.3 3,466,559 - +0.4 3,466,559 - +0.4 3,466,559 - -0.1 3,466,559 - -0.2 3,466,560 + +1.0 3,466,560 + +0.3 3,466,560 + -1.8 3,466,560 + -0.5 3,466,562 + -2.3 3,466,959 + -2.7 3,466,989 + -1.5 3,466,989 + -1.8 3,466,989 + -0.2 3,467,018 - +0.5 3,467,030 + -1.1 3,467,163 + -0.1 3,467,163 + +1.5 3,467,163 + +1.5 3,467,163 + +2.0 3,467,224 + +1.0 3,467,225 - -1.5 3,467,242 - +1.1 3,467,264 - +0.8 3,467,264 - -0.2 3,467,264 - -1.0 3,467,296 + -0.0 3,467,296 + -0.6 3,467,350 - -2.6 3,467,350 - -0.5 3,467,418 + +0.8 3,467,419 - +1.0 3,467,419 - +0.7 3,467,419 - -1.0 3,467,419 - -1.6 3,467,441 + -0.4 3,467,441 + +2.0 3,467,590 + Pf6N2E2_2975 0.10 -0.6
Or see this region's nucleotide sequence