Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2424 and Pf6N2E2_2425 are separated by 75 nucleotides Pf6N2E2_2425 and Pf6N2E2_2426 are separated by 78 nucleotides Pf6N2E2_2426 and Pf6N2E2_2427 are separated by 72 nucleotides Pf6N2E2_2427 and Pf6N2E2_2428 are separated by 92 nucleotides Pf6N2E2_2428 and Pf6N2E2_2429 are separated by 189 nucleotides
Pf6N2E2_2424 - Molybdopterin-guanine dinucleotide biosynthesis protein MobA, at 2,874,663 to 2,875,265
_2424
Pf6N2E2_2425 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 2,875,341 to 2,875,562
_2425
Pf6N2E2_2426 - Similar to ribosomal large subunit pseudouridine synthase A, at 2,875,641 to 2,876,531
_2426
Pf6N2E2_2427 - DNA damage response gene with a putative DNA binding domain and often regulated by LexA (PA0922-like) (from data) , at 2,876,604 to 2,876,924
_2427
Pf6N2E2_2428 - Thiol-disulfide isomerase and thioredoxins, at 2,877,017 to 2,877,253
_2428
Pf6N2E2_2429 - Glutathione-regulated potassium-efflux system protein KefB, at 2,877,443 to 2,879,155
_2429
Position (kb)
2875
2876
2877 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2874.840 kb on - strand, within Pf6N2E2_2424 at 2874.861 kb on + strand, within Pf6N2E2_2424 at 2874.861 kb on + strand, within Pf6N2E2_2424 at 2874.861 kb on + strand, within Pf6N2E2_2424 at 2874.862 kb on - strand, within Pf6N2E2_2424 at 2874.951 kb on + strand, within Pf6N2E2_2424 at 2874.952 kb on - strand, within Pf6N2E2_2424 at 2875.235 kb on - strand at 2875.247 kb on + strand at 2875.247 kb on + strand at 2875.248 kb on - strand at 2875.269 kb on + strand at 2875.292 kb on + strand at 2875.292 kb on + strand at 2875.292 kb on + strand at 2875.292 kb on + strand at 2875.292 kb on + strand at 2875.292 kb on + strand at 2875.292 kb on + strand at 2875.292 kb on + strand at 2875.292 kb on + strand at 2875.292 kb on + strand at 2875.293 kb on - strand at 2875.293 kb on - strand at 2875.293 kb on - strand at 2875.293 kb on - strand at 2875.293 kb on - strand at 2875.293 kb on - strand at 2875.294 kb on + strand at 2875.294 kb on + strand at 2875.294 kb on + strand at 2875.295 kb on - strand at 2875.295 kb on - strand at 2875.295 kb on - strand at 2875.295 kb on - strand at 2875.310 kb on + strand at 2875.467 kb on + strand, within Pf6N2E2_2425 at 2875.467 kb on + strand, within Pf6N2E2_2425 at 2875.488 kb on + strand, within Pf6N2E2_2425 at 2875.488 kb on + strand, within Pf6N2E2_2425 at 2875.489 kb on - strand, within Pf6N2E2_2425 at 2875.544 kb on + strand at 2875.544 kb on + strand at 2875.545 kb on - strand at 2875.560 kb on + strand at 2875.561 kb on - strand at 2875.584 kb on - strand at 2875.616 kb on - strand at 2875.616 kb on - strand at 2875.654 kb on - strand at 2875.654 kb on - strand at 2875.654 kb on - strand at 2875.654 kb on - strand at 2875.671 kb on + strand at 2875.671 kb on + strand at 2875.671 kb on + strand at 2875.672 kb on - strand at 2875.672 kb on - strand at 2875.722 kb on + strand at 2875.774 kb on + strand, within Pf6N2E2_2426 at 2875.775 kb on - strand, within Pf6N2E2_2426 at 2875.775 kb on - strand, within Pf6N2E2_2426 at 2875.852 kb on + strand, within Pf6N2E2_2426 at 2875.973 kb on + strand, within Pf6N2E2_2426 at 2875.973 kb on + strand, within Pf6N2E2_2426 at 2875.973 kb on + strand, within Pf6N2E2_2426 at 2875.973 kb on + strand, within Pf6N2E2_2426 at 2875.973 kb on + strand, within Pf6N2E2_2426 at 2875.973 kb on + strand, within Pf6N2E2_2426 at 2875.973 kb on + strand, within Pf6N2E2_2426 at 2875.973 kb on + strand, within Pf6N2E2_2426 at 2875.973 kb on + strand, within Pf6N2E2_2426 at 2875.973 kb on + strand, within Pf6N2E2_2426 at 2875.973 kb on + strand, within Pf6N2E2_2426 at 2875.973 kb on + strand, within Pf6N2E2_2426 at 2875.973 kb on + strand, within Pf6N2E2_2426 at 2875.974 kb on - strand, within Pf6N2E2_2426 at 2875.974 kb on - strand, within Pf6N2E2_2426 at 2875.974 kb on - strand, within Pf6N2E2_2426 at 2875.974 kb on - strand, within Pf6N2E2_2426 at 2875.974 kb on - strand, within Pf6N2E2_2426 at 2875.974 kb on - strand, within Pf6N2E2_2426 at 2875.974 kb on - strand, within Pf6N2E2_2426 at 2875.974 kb on - strand, within Pf6N2E2_2426 at 2875.974 kb on - strand, within Pf6N2E2_2426 at 2875.974 kb on - strand, within Pf6N2E2_2426 at 2875.974 kb on - strand, within Pf6N2E2_2426 at 2875.974 kb on - strand, within Pf6N2E2_2426 at 2875.974 kb on - strand, within Pf6N2E2_2426 at 2876.062 kb on + strand, within Pf6N2E2_2426 at 2876.063 kb on - strand, within Pf6N2E2_2426 at 2876.261 kb on - strand, within Pf6N2E2_2426 at 2876.315 kb on - strand, within Pf6N2E2_2426 at 2876.315 kb on - strand, within Pf6N2E2_2426 at 2876.482 kb on + strand at 2876.482 kb on + strand at 2876.482 kb on + strand at 2876.489 kb on + strand at 2876.490 kb on - strand at 2876.490 kb on - strand at 2876.542 kb on + strand at 2876.563 kb on + strand at 2876.564 kb on - strand at 2876.564 kb on - strand at 2876.564 kb on - strand at 2876.695 kb on + strand, within Pf6N2E2_2427 at 2876.695 kb on + strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.704 kb on + strand, within Pf6N2E2_2427 at 2876.704 kb on + strand, within Pf6N2E2_2427 at 2876.705 kb on - strand, within Pf6N2E2_2427 at 2876.705 kb on - strand, within Pf6N2E2_2427 at 2876.705 kb on - strand, within Pf6N2E2_2427 at 2876.941 kb on + strand at 2876.993 kb on - strand at 2877.111 kb on + strand, within Pf6N2E2_2428 at 2877.111 kb on + strand, within Pf6N2E2_2428 at 2877.111 kb on + strand, within Pf6N2E2_2428 at 2877.200 kb on + strand, within Pf6N2E2_2428 at 2877.288 kb on + strand at 2877.288 kb on + strand at 2877.407 kb on + strand at 2877.421 kb on + strand at 2877.421 kb on + strand at 2877.530 kb on + strand at 2877.531 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 2,874,840 - Pf6N2E2_2424 0.29 +0.4 2,874,861 + Pf6N2E2_2424 0.33 +0.3 2,874,861 + Pf6N2E2_2424 0.33 -3.1 2,874,861 + Pf6N2E2_2424 0.33 -1.5 2,874,862 - Pf6N2E2_2424 0.33 -1.4 2,874,951 + Pf6N2E2_2424 0.48 -1.2 2,874,952 - Pf6N2E2_2424 0.48 -0.2 2,875,235 - -0.7 2,875,247 + +0.6 2,875,247 + +0.5 2,875,248 - +0.2 2,875,269 + -1.5 2,875,292 + +0.8 2,875,292 + +0.8 2,875,292 + +0.8 2,875,292 + +0.7 2,875,292 + +1.2 2,875,292 + +0.0 2,875,292 + +0.1 2,875,292 + -2.9 2,875,292 + -0.2 2,875,292 + +0.4 2,875,293 - -2.7 2,875,293 - -0.2 2,875,293 - -1.8 2,875,293 - -2.7 2,875,293 - +0.5 2,875,293 - -3.4 2,875,294 + +0.0 2,875,294 + +1.2 2,875,294 + +0.8 2,875,295 - -0.2 2,875,295 - -3.3 2,875,295 - -1.0 2,875,295 - -1.7 2,875,310 + -0.2 2,875,467 + Pf6N2E2_2425 0.57 -1.7 2,875,467 + Pf6N2E2_2425 0.57 -0.7 2,875,488 + Pf6N2E2_2425 0.66 -1.2 2,875,488 + Pf6N2E2_2425 0.66 -2.9 2,875,489 - Pf6N2E2_2425 0.67 -1.6 2,875,544 + -1.5 2,875,544 + -1.4 2,875,545 - +0.8 2,875,560 + -0.7 2,875,561 - -1.5 2,875,584 - -1.9 2,875,616 - +0.8 2,875,616 - +0.8 2,875,654 - -0.3 2,875,654 - +3.1 2,875,654 - +0.6 2,875,654 - -0.3 2,875,671 + -0.8 2,875,671 + +0.8 2,875,671 + +1.9 2,875,672 - +1.5 2,875,672 - +1.7 2,875,722 + +1.9 2,875,774 + Pf6N2E2_2426 0.15 +0.8 2,875,775 - Pf6N2E2_2426 0.15 +2.1 2,875,775 - Pf6N2E2_2426 0.15 +1.8 2,875,852 + Pf6N2E2_2426 0.24 +4.0 2,875,973 + Pf6N2E2_2426 0.37 +1.6 2,875,973 + Pf6N2E2_2426 0.37 +1.4 2,875,973 + Pf6N2E2_2426 0.37 -0.0 2,875,973 + Pf6N2E2_2426 0.37 +1.5 2,875,973 + Pf6N2E2_2426 0.37 +2.5 2,875,973 + Pf6N2E2_2426 0.37 +1.5 2,875,973 + Pf6N2E2_2426 0.37 +2.2 2,875,973 + Pf6N2E2_2426 0.37 -0.4 2,875,973 + Pf6N2E2_2426 0.37 -2.4 2,875,973 + Pf6N2E2_2426 0.37 -0.2 2,875,973 + Pf6N2E2_2426 0.37 +1.9 2,875,973 + Pf6N2E2_2426 0.37 -1.5 2,875,973 + Pf6N2E2_2426 0.37 +1.8 2,875,974 - Pf6N2E2_2426 0.37 +1.4 2,875,974 - Pf6N2E2_2426 0.37 +0.6 2,875,974 - Pf6N2E2_2426 0.37 +2.0 2,875,974 - Pf6N2E2_2426 0.37 +3.2 2,875,974 - Pf6N2E2_2426 0.37 +0.3 2,875,974 - Pf6N2E2_2426 0.37 -1.5 2,875,974 - Pf6N2E2_2426 0.37 +3.1 2,875,974 - Pf6N2E2_2426 0.37 +2.7 2,875,974 - Pf6N2E2_2426 0.37 +0.8 2,875,974 - Pf6N2E2_2426 0.37 +2.6 2,875,974 - Pf6N2E2_2426 0.37 +0.2 2,875,974 - Pf6N2E2_2426 0.37 -0.2 2,875,974 - Pf6N2E2_2426 0.37 +1.0 2,876,062 + Pf6N2E2_2426 0.47 +1.4 2,876,063 - Pf6N2E2_2426 0.47 +1.2 2,876,261 - Pf6N2E2_2426 0.70 +1.3 2,876,315 - Pf6N2E2_2426 0.76 +1.4 2,876,315 - Pf6N2E2_2426 0.76 -0.8 2,876,482 + +1.1 2,876,482 + +4.1 2,876,482 + +1.3 2,876,489 + +0.6 2,876,490 - +0.2 2,876,490 - +2.3 2,876,542 + +0.8 2,876,563 + +0.0 2,876,564 - +1.0 2,876,564 - +1.5 2,876,564 - +0.1 2,876,695 + Pf6N2E2_2427 0.28 -2.1 2,876,695 + Pf6N2E2_2427 0.28 -0.0 2,876,696 - Pf6N2E2_2427 0.29 +0.8 2,876,696 - Pf6N2E2_2427 0.29 -0.6 2,876,696 - Pf6N2E2_2427 0.29 -1.7 2,876,696 - Pf6N2E2_2427 0.29 -0.1 2,876,696 - Pf6N2E2_2427 0.29 -1.0 2,876,696 - Pf6N2E2_2427 0.29 -0.2 2,876,696 - Pf6N2E2_2427 0.29 -1.8 2,876,696 - Pf6N2E2_2427 0.29 -0.5 2,876,696 - Pf6N2E2_2427 0.29 -0.1 2,876,696 - Pf6N2E2_2427 0.29 +1.6 2,876,704 + Pf6N2E2_2427 0.31 +0.4 2,876,704 + Pf6N2E2_2427 0.31 -0.3 2,876,705 - Pf6N2E2_2427 0.31 +0.8 2,876,705 - Pf6N2E2_2427 0.31 -0.2 2,876,705 - Pf6N2E2_2427 0.31 -1.2 2,876,941 + -0.2 2,876,993 - +0.6 2,877,111 + Pf6N2E2_2428 0.40 +0.5 2,877,111 + Pf6N2E2_2428 0.40 +0.1 2,877,111 + Pf6N2E2_2428 0.40 -0.5 2,877,200 + Pf6N2E2_2428 0.77 -0.2 2,877,288 + +0.1 2,877,288 + -0.3 2,877,407 + -0.2 2,877,421 + -0.9 2,877,421 + -0.3 2,877,530 + -0.8 2,877,531 - +1.4
Or see this region's nucleotide sequence