Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1899 and Pf6N2E2_1900 are separated by 361 nucleotides Pf6N2E2_1900 and Pf6N2E2_1901 are separated by 105 nucleotides Pf6N2E2_1901 and Pf6N2E2_1902 are separated by 59 nucleotides
Pf6N2E2_1899 - Dienelactone hydrolase and related enzymes, at 2,250,537 to 2,251,511
_1899
Pf6N2E2_1900 - hypothetical protein, at 2,251,873 to 2,252,337
_1900
Pf6N2E2_1901 - Sorbitol-6-phosphate 2-dehydrogenase (EC 1.1.1.140), at 2,252,443 to 2,253,186
_1901
Pf6N2E2_1902 - Oxidoreductase, aldo/keto reductase family, at 2,253,246 to 2,254,196
_1902
Position (kb)
2252
2253
2254 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2251.579 kb on - strand at 2251.583 kb on + strand at 2251.645 kb on + strand at 2251.645 kb on + strand at 2251.646 kb on - strand at 2251.646 kb on - strand at 2251.646 kb on - strand at 2251.646 kb on - strand at 2251.646 kb on - strand at 2251.646 kb on - strand at 2251.668 kb on - strand at 2251.676 kb on - strand at 2251.981 kb on - strand, within Pf6N2E2_1900 at 2252.190 kb on + strand, within Pf6N2E2_1900 at 2252.190 kb on + strand, within Pf6N2E2_1900 at 2252.191 kb on - strand, within Pf6N2E2_1900 at 2252.191 kb on - strand, within Pf6N2E2_1900 at 2252.191 kb on - strand, within Pf6N2E2_1900 at 2252.224 kb on + strand, within Pf6N2E2_1900 at 2252.224 kb on + strand, within Pf6N2E2_1900 at 2252.225 kb on - strand, within Pf6N2E2_1900 at 2252.245 kb on + strand, within Pf6N2E2_1900 at 2252.246 kb on - strand, within Pf6N2E2_1900 at 2252.251 kb on + strand, within Pf6N2E2_1900 at 2252.251 kb on + strand, within Pf6N2E2_1900 at 2252.251 kb on + strand, within Pf6N2E2_1900 at 2252.251 kb on + strand, within Pf6N2E2_1900 at 2252.251 kb on + strand at 2252.251 kb on + strand, within Pf6N2E2_1900 at 2252.252 kb on - strand, within Pf6N2E2_1900 at 2252.356 kb on + strand at 2252.356 kb on + strand at 2252.356 kb on + strand at 2252.459 kb on + strand at 2252.459 kb on + strand at 2252.459 kb on + strand at 2252.459 kb on + strand at 2252.459 kb on + strand at 2252.459 kb on + strand at 2252.612 kb on - strand, within Pf6N2E2_1901 at 2252.768 kb on + strand, within Pf6N2E2_1901 at 2252.768 kb on + strand, within Pf6N2E2_1901 at 2252.768 kb on + strand, within Pf6N2E2_1901 at 2252.768 kb on + strand, within Pf6N2E2_1901 at 2252.769 kb on - strand, within Pf6N2E2_1901 at 2252.769 kb on - strand, within Pf6N2E2_1901 at 2252.853 kb on + strand, within Pf6N2E2_1901 at 2252.854 kb on - strand, within Pf6N2E2_1901 at 2252.873 kb on + strand, within Pf6N2E2_1901 at 2252.992 kb on + strand, within Pf6N2E2_1901 at 2253.067 kb on + strand, within Pf6N2E2_1901 at 2253.067 kb on + strand, within Pf6N2E2_1901 at 2253.067 kb on + strand, within Pf6N2E2_1901 at 2253.067 kb on + strand, within Pf6N2E2_1901 at 2253.067 kb on + strand, within Pf6N2E2_1901 at 2253.067 kb on + strand, within Pf6N2E2_1901 at 2253.068 kb on - strand, within Pf6N2E2_1901 at 2253.068 kb on - strand, within Pf6N2E2_1901 at 2253.068 kb on - strand, within Pf6N2E2_1901 at 2253.068 kb on - strand, within Pf6N2E2_1901 at 2253.068 kb on - strand, within Pf6N2E2_1901 at 2253.077 kb on - strand, within Pf6N2E2_1901 at 2253.077 kb on - strand, within Pf6N2E2_1901 at 2253.257 kb on - strand at 2253.348 kb on + strand, within Pf6N2E2_1902 at 2253.349 kb on - strand, within Pf6N2E2_1902 at 2253.535 kb on - strand, within Pf6N2E2_1902 at 2253.547 kb on - strand, within Pf6N2E2_1902 at 2253.600 kb on + strand, within Pf6N2E2_1902 at 2253.614 kb on - strand, within Pf6N2E2_1902 at 2253.614 kb on - strand, within Pf6N2E2_1902 at 2253.614 kb on - strand, within Pf6N2E2_1902 at 2253.720 kb on - strand, within Pf6N2E2_1902 at 2253.858 kb on - strand, within Pf6N2E2_1902 at 2253.983 kb on + strand, within Pf6N2E2_1902 at 2253.983 kb on + strand, within Pf6N2E2_1902 at 2254.014 kb on + strand, within Pf6N2E2_1902 at 2254.015 kb on - strand, within Pf6N2E2_1902 at 2254.134 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 2,251,579 - +0.6 2,251,583 + +1.3 2,251,645 + +2.9 2,251,645 + -1.2 2,251,646 - +0.6 2,251,646 - +0.5 2,251,646 - -0.5 2,251,646 - +1.1 2,251,646 - -0.4 2,251,646 - +0.8 2,251,668 - -0.4 2,251,676 - -0.7 2,251,981 - Pf6N2E2_1900 0.23 -0.6 2,252,190 + Pf6N2E2_1900 0.68 -0.2 2,252,190 + Pf6N2E2_1900 0.68 -0.3 2,252,191 - Pf6N2E2_1900 0.68 -2.2 2,252,191 - Pf6N2E2_1900 0.68 -2.0 2,252,191 - Pf6N2E2_1900 0.68 +0.8 2,252,224 + Pf6N2E2_1900 0.75 +0.0 2,252,224 + Pf6N2E2_1900 0.75 -2.4 2,252,225 - Pf6N2E2_1900 0.76 -0.1 2,252,245 + Pf6N2E2_1900 0.80 -0.4 2,252,246 - Pf6N2E2_1900 0.80 +0.6 2,252,251 + Pf6N2E2_1900 0.81 -1.2 2,252,251 + Pf6N2E2_1900 0.81 +0.6 2,252,251 + Pf6N2E2_1900 0.81 -0.8 2,252,251 + Pf6N2E2_1900 0.81 -0.5 2,252,251 + +0.6 2,252,251 + Pf6N2E2_1900 0.81 -2.2 2,252,252 - Pf6N2E2_1900 0.82 +0.5 2,252,356 + +0.6 2,252,356 + -2.4 2,252,356 + +0.6 2,252,459 + +1.2 2,252,459 + +1.2 2,252,459 + -1.7 2,252,459 + +0.6 2,252,459 + -0.3 2,252,459 + -1.8 2,252,612 - Pf6N2E2_1901 0.23 +1.5 2,252,768 + Pf6N2E2_1901 0.44 +0.5 2,252,768 + Pf6N2E2_1901 0.44 -0.7 2,252,768 + Pf6N2E2_1901 0.44 +0.1 2,252,768 + Pf6N2E2_1901 0.44 -2.0 2,252,769 - Pf6N2E2_1901 0.44 +0.9 2,252,769 - Pf6N2E2_1901 0.44 +0.7 2,252,853 + Pf6N2E2_1901 0.55 +0.3 2,252,854 - Pf6N2E2_1901 0.55 -3.3 2,252,873 + Pf6N2E2_1901 0.58 +0.1 2,252,992 + Pf6N2E2_1901 0.74 -0.3 2,253,067 + Pf6N2E2_1901 0.84 -0.1 2,253,067 + Pf6N2E2_1901 0.84 +2.2 2,253,067 + Pf6N2E2_1901 0.84 -0.4 2,253,067 + Pf6N2E2_1901 0.84 +0.3 2,253,067 + Pf6N2E2_1901 0.84 -0.8 2,253,067 + Pf6N2E2_1901 0.84 +0.1 2,253,068 - Pf6N2E2_1901 0.84 +0.0 2,253,068 - Pf6N2E2_1901 0.84 -0.7 2,253,068 - Pf6N2E2_1901 0.84 +2.1 2,253,068 - Pf6N2E2_1901 0.84 -2.0 2,253,068 - Pf6N2E2_1901 0.84 -0.7 2,253,077 - Pf6N2E2_1901 0.85 +0.1 2,253,077 - Pf6N2E2_1901 0.85 +0.6 2,253,257 - -0.8 2,253,348 + Pf6N2E2_1902 0.11 +0.6 2,253,349 - Pf6N2E2_1902 0.11 -3.1 2,253,535 - Pf6N2E2_1902 0.30 -0.4 2,253,547 - Pf6N2E2_1902 0.32 -2.4 2,253,600 + Pf6N2E2_1902 0.37 +0.6 2,253,614 - Pf6N2E2_1902 0.39 +1.2 2,253,614 - Pf6N2E2_1902 0.39 -0.4 2,253,614 - Pf6N2E2_1902 0.39 +0.2 2,253,720 - Pf6N2E2_1902 0.50 +1.0 2,253,858 - Pf6N2E2_1902 0.64 +1.4 2,253,983 + Pf6N2E2_1902 0.77 +0.6 2,253,983 + Pf6N2E2_1902 0.77 +0.4 2,254,014 + Pf6N2E2_1902 0.81 +0.7 2,254,015 - Pf6N2E2_1902 0.81 +0.3 2,254,134 - +0.4
Or see this region's nucleotide sequence