Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1107 and Pf6N2E2_1108 are separated by 3 nucleotides Pf6N2E2_1108 and Pf6N2E2_1109 are separated by 158 nucleotides
Pf6N2E2_1107 - hypothetical protein, at 1,294,263 to 1,299,359
_1107
Pf6N2E2_1108 - Methyltransferase (EC 2.1.1.-), at 1,299,363 to 1,300,421
_1108
Pf6N2E2_1109 - Mobile element protein, at 1,300,580 to 1,301,818
_1109
Position (kb)
1299
1300
1301 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1298.407 kb on - strand, within Pf6N2E2_1107 at 1298.589 kb on + strand, within Pf6N2E2_1107 at 1298.589 kb on + strand, within Pf6N2E2_1107 at 1298.590 kb on - strand, within Pf6N2E2_1107 at 1298.590 kb on - strand, within Pf6N2E2_1107 at 1298.592 kb on + strand, within Pf6N2E2_1107 at 1298.592 kb on + strand, within Pf6N2E2_1107 at 1298.592 kb on + strand, within Pf6N2E2_1107 at 1298.593 kb on - strand, within Pf6N2E2_1107 at 1298.613 kb on + strand, within Pf6N2E2_1107 at 1298.613 kb on + strand, within Pf6N2E2_1107 at 1298.613 kb on + strand, within Pf6N2E2_1107 at 1298.614 kb on - strand, within Pf6N2E2_1107 at 1298.614 kb on - strand, within Pf6N2E2_1107 at 1298.614 kb on - strand, within Pf6N2E2_1107 at 1298.614 kb on - strand, within Pf6N2E2_1107 at 1298.614 kb on - strand, within Pf6N2E2_1107 at 1298.614 kb on - strand, within Pf6N2E2_1107 at 1298.614 kb on - strand, within Pf6N2E2_1107 at 1298.818 kb on - strand, within Pf6N2E2_1107 at 1298.831 kb on - strand, within Pf6N2E2_1107 at 1299.088 kb on + strand at 1299.267 kb on + strand at 1299.267 kb on + strand at 1299.267 kb on + strand at 1299.267 kb on + strand at 1299.267 kb on + strand at 1299.267 kb on + strand at 1299.268 kb on - strand at 1299.268 kb on - strand at 1299.268 kb on - strand at 1299.268 kb on - strand at 1299.268 kb on - strand at 1299.357 kb on + strand at 1299.667 kb on + strand, within Pf6N2E2_1108 at 1300.049 kb on - strand, within Pf6N2E2_1108 at 1300.059 kb on + strand, within Pf6N2E2_1108 at 1300.070 kb on + strand, within Pf6N2E2_1108 at 1300.103 kb on + strand, within Pf6N2E2_1108 at 1300.103 kb on + strand, within Pf6N2E2_1108 at 1300.103 kb on + strand, within Pf6N2E2_1108 at 1300.104 kb on - strand, within Pf6N2E2_1108 at 1300.359 kb on + strand at 1300.359 kb on + strand at 1300.360 kb on - strand at 1300.360 kb on - strand at 1300.462 kb on - strand at 1300.490 kb on - strand at 1300.490 kb on - strand at 1300.527 kb on - strand at 1300.536 kb on - strand at 1300.548 kb on + strand at 1300.644 kb on + strand at 1300.645 kb on - strand at 1300.906 kb on - strand, within Pf6N2E2_1109 at 1300.906 kb on - strand, within Pf6N2E2_1109 at 1300.978 kb on - strand, within Pf6N2E2_1109 at 1300.978 kb on - strand, within Pf6N2E2_1109 at 1300.978 kb on - strand, within Pf6N2E2_1109 at 1300.978 kb on - strand, within Pf6N2E2_1109 at 1300.978 kb on - strand, within Pf6N2E2_1109 at 1301.064 kb on + strand, within Pf6N2E2_1109 at 1301.065 kb on - strand, within Pf6N2E2_1109 at 1301.065 kb on - strand, within Pf6N2E2_1109 at 1301.065 kb on - strand, within Pf6N2E2_1109 at 1301.065 kb on - strand, within Pf6N2E2_1109 at 1301.065 kb on - strand, within Pf6N2E2_1109 at 1301.116 kb on - strand, within Pf6N2E2_1109 at 1301.186 kb on - strand, within Pf6N2E2_1109 at 1301.363 kb on + strand, within Pf6N2E2_1109
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 1,298,407 - Pf6N2E2_1107 0.81 +1.1 1,298,589 + Pf6N2E2_1107 0.85 +0.3 1,298,589 + Pf6N2E2_1107 0.85 +0.9 1,298,590 - Pf6N2E2_1107 0.85 +0.9 1,298,590 - Pf6N2E2_1107 0.85 -0.2 1,298,592 + Pf6N2E2_1107 0.85 -3.6 1,298,592 + Pf6N2E2_1107 0.85 -2.0 1,298,592 + Pf6N2E2_1107 0.85 -1.3 1,298,593 - Pf6N2E2_1107 0.85 -0.4 1,298,613 + Pf6N2E2_1107 0.85 +0.7 1,298,613 + Pf6N2E2_1107 0.85 +1.3 1,298,613 + Pf6N2E2_1107 0.85 +1.1 1,298,614 - Pf6N2E2_1107 0.85 +0.6 1,298,614 - Pf6N2E2_1107 0.85 +0.1 1,298,614 - Pf6N2E2_1107 0.85 +0.7 1,298,614 - Pf6N2E2_1107 0.85 +0.4 1,298,614 - Pf6N2E2_1107 0.85 +0.9 1,298,614 - Pf6N2E2_1107 0.85 +1.2 1,298,614 - Pf6N2E2_1107 0.85 -0.3 1,298,818 - Pf6N2E2_1107 0.89 -0.7 1,298,831 - Pf6N2E2_1107 0.90 -0.7 1,299,088 + -2.5 1,299,267 + -0.3 1,299,267 + +1.1 1,299,267 + +0.3 1,299,267 + +0.5 1,299,267 + +0.1 1,299,267 + +0.7 1,299,268 - -2.5 1,299,268 - -1.9 1,299,268 - -1.5 1,299,268 - -0.3 1,299,268 - +0.3 1,299,357 + -2.3 1,299,667 + Pf6N2E2_1108 0.29 +0.3 1,300,049 - Pf6N2E2_1108 0.65 +1.2 1,300,059 + Pf6N2E2_1108 0.66 +1.0 1,300,070 + Pf6N2E2_1108 0.67 +0.7 1,300,103 + Pf6N2E2_1108 0.70 +1.9 1,300,103 + Pf6N2E2_1108 0.70 +1.7 1,300,103 + Pf6N2E2_1108 0.70 +1.2 1,300,104 - Pf6N2E2_1108 0.70 -1.3 1,300,359 + +0.3 1,300,359 + +0.2 1,300,360 - -0.4 1,300,360 - -0.8 1,300,462 - -0.7 1,300,490 - +0.3 1,300,490 - +0.6 1,300,527 - -0.5 1,300,536 - -1.0 1,300,548 + -1.7 1,300,644 + +0.1 1,300,645 - +0.3 1,300,906 - Pf6N2E2_1109 0.26 +0.8 1,300,906 - Pf6N2E2_1109 0.26 -1.7 1,300,978 - Pf6N2E2_1109 0.32 -1.5 1,300,978 - Pf6N2E2_1109 0.32 -0.4 1,300,978 - Pf6N2E2_1109 0.32 -0.7 1,300,978 - Pf6N2E2_1109 0.32 +0.1 1,300,978 - Pf6N2E2_1109 0.32 -0.6 1,301,064 + Pf6N2E2_1109 0.39 -0.4 1,301,065 - Pf6N2E2_1109 0.39 -0.2 1,301,065 - Pf6N2E2_1109 0.39 +0.5 1,301,065 - Pf6N2E2_1109 0.39 +2.1 1,301,065 - Pf6N2E2_1109 0.39 +1.3 1,301,065 - Pf6N2E2_1109 0.39 -0.7 1,301,116 - Pf6N2E2_1109 0.43 +0.9 1,301,186 - Pf6N2E2_1109 0.49 -0.9 1,301,363 + Pf6N2E2_1109 0.63 -1.6
Or see this region's nucleotide sequence