Experiment: Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2740 and tRNA-Thr are separated by 50 nucleotides tRNA-Thr and tRNA-Gly are separated by 12 nucleotides tRNA-Gly and tRNA-Tyr are separated by 25 nucleotides tRNA-Tyr and tRNA-Thr are separated by 155 nucleotides tRNA-Thr and BT2741 are separated by 97 nucleotides BT2741 and BT2742 are separated by 16 nucleotides BT2742 and BT2743 are separated by 456 nucleotides
BT2740: BT2740 - elongation factor Tu (NCBI ptt file), at 3,393,922 to 3,395,106
BT2740
BT_t45: tRNA-Thr - tRNA-Thr (RefSeq), at 3,395,157 to 3,395,228
tRNA-Thr
BT_t46: tRNA-Gly - tRNA-Gly (RefSeq), at 3,395,241 to 3,395,313
tRNA-Gly
BT_t47: tRNA-Tyr - tRNA-Tyr (RefSeq), at 3,395,339 to 3,395,424
tRNA-Tyr
BT_t48: tRNA-Thr - tRNA-Thr (RefSeq), at 3,395,580 to 3,395,656
tRNA-Thr
BT2741: BT2741 - sigma-54 modulation protein (NCBI ptt file), at 3,395,754 to 3,396,053
BT2741
BT2742: BT2742 - integrase, site-specific recombinase (NCBI ptt file), at 3,396,070 to 3,396,951
BT2742
BT2743: BT2743 - putative aminopeptidase (NCBI ptt file), at 3,397,408 to 3,399,189
BT2743
Position (kb)
3396
3397 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3395.763 kb on - strand at 3395.763 kb on - strand at 3395.838 kb on - strand, within BT2741 at 3395.849 kb on + strand, within BT2741 at 3395.850 kb on - strand, within BT2741 at 3395.921 kb on + strand, within BT2741 at 3395.921 kb on + strand, within BT2741 at 3395.944 kb on - strand, within BT2741 at 3395.944 kb on - strand at 3396.045 kb on - strand at 3396.054 kb on - strand at 3396.054 kb on - strand at 3396.058 kb on - strand at 3396.058 kb on - strand at 3396.223 kb on - strand, within BT2742 at 3397.284 kb on - strand at 3397.360 kb on + strand at 3397.361 kb on - strand at 3397.364 kb on - strand at 3397.383 kb on - strand at 3397.399 kb on + strand at 3397.400 kb on - strand at 3397.402 kb on + strand at 3397.421 kb on + strand at 3397.467 kb on + strand at 3397.513 kb on + strand at 3397.514 kb on - strand at 3397.514 kb on - strand at 3397.518 kb on - strand at 3397.518 kb on - strand at 3397.586 kb on + strand at 3397.587 kb on - strand, within BT2743 at 3397.593 kb on + strand, within BT2743 at 3397.594 kb on - strand, within BT2743 at 3397.662 kb on + strand, within BT2743 at 3397.662 kb on + strand, within BT2743 at 3397.669 kb on + strand, within BT2743 at 3397.671 kb on + strand, within BT2743 at 3397.672 kb on - strand, within BT2743 at 3397.725 kb on - strand, within BT2743 at 3397.746 kb on + strand, within BT2743 at 3397.747 kb on - strand, within BT2743 at 3397.769 kb on + strand, within BT2743 at 3397.895 kb on - strand, within BT2743 at 3397.906 kb on + strand, within BT2743 at 3397.906 kb on + strand, within BT2743
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM remove 3,395,763 - -0.8 3,395,763 - -0.9 3,395,838 - BT2741 0.28 -2.7 3,395,849 + BT2741 0.32 +0.1 3,395,850 - BT2741 0.32 -1.0 3,395,921 + BT2741 0.56 -2.5 3,395,921 + BT2741 0.56 +0.1 3,395,944 - BT2741 0.63 +0.1 3,395,944 - +0.1 3,396,045 - +0.2 3,396,054 - -0.7 3,396,054 - +0.1 3,396,058 - -0.6 3,396,058 - -1.9 3,396,223 - BT2742 0.17 -3.4 3,397,284 - +0.8 3,397,360 + -0.9 3,397,361 - +1.1 3,397,364 - +0.4 3,397,383 - -1.4 3,397,399 + -1.2 3,397,400 - -1.2 3,397,402 + +0.7 3,397,421 + +0.1 3,397,467 + +0.1 3,397,513 + +0.1 3,397,514 - -0.3 3,397,514 - -2.5 3,397,518 - -0.7 3,397,518 - -0.9 3,397,586 + +1.7 3,397,587 - BT2743 0.10 +0.8 3,397,593 + BT2743 0.10 -0.9 3,397,594 - BT2743 0.10 +1.9 3,397,662 + BT2743 0.14 +0.1 3,397,662 + BT2743 0.14 +0.7 3,397,669 + BT2743 0.15 -0.9 3,397,671 + BT2743 0.15 -1.9 3,397,672 - BT2743 0.15 +0.1 3,397,725 - BT2743 0.18 +2.9 3,397,746 + BT2743 0.19 +0.1 3,397,747 - BT2743 0.19 +0.4 3,397,769 + BT2743 0.20 -4.7 3,397,895 - BT2743 0.27 -0.1 3,397,906 + BT2743 0.28 +0.8 3,397,906 + BT2743 0.28 -0.3
Or see this region's nucleotide sequence