Experiment: Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2544 and BT2545 are separated by 12 nucleotides BT2545 and BT2546 are separated by 22 nucleotides BT2546 and BT2547 are separated by 118 nucleotides BT2547 and BT2548 are separated by 30 nucleotides
BT2544: BT2544 - putative metal-dependent membrane protease (NCBI ptt file), at 3,178,471 to 3,179,274
BT2544
BT2545: BT2545 - hypothetical protein (NCBI ptt file), at 3,179,287 to 3,179,943
BT2545
BT2546: BT2546 - transcriptional regulator (NCBI ptt file), at 3,179,966 to 3,180,181
BT2546
BT2547: BT2547 - conserved hypothetical protein (NCBI ptt file), at 3,180,300 to 3,180,725
BT2547
BT2548: BT2548 - leucine aminopeptidase precursor (NCBI ptt file), at 3,180,756 to 3,181,751
BT2548
Position (kb)
3179
3180
3181 Strain fitness (log2 ratio)
-2
-1
0
1 at 3179.208 kb on + strand at 3179.208 kb on + strand at 3179.239 kb on + strand at 3179.262 kb on + strand at 3179.439 kb on + strand, within BT2545 at 3179.474 kb on + strand, within BT2545 at 3179.584 kb on + strand, within BT2545 at 3179.585 kb on - strand, within BT2545 at 3179.649 kb on + strand, within BT2545 at 3179.745 kb on + strand, within BT2545 at 3179.745 kb on + strand, within BT2545 at 3179.821 kb on + strand, within BT2545 at 3179.821 kb on + strand, within BT2545 at 3179.823 kb on + strand, within BT2545 at 3179.974 kb on + strand at 3180.064 kb on + strand, within BT2546 at 3180.148 kb on + strand, within BT2546 at 3180.180 kb on + strand at 3180.267 kb on - strand at 3180.267 kb on - strand at 3180.301 kb on + strand at 3180.302 kb on - strand at 3180.746 kb on - strand at 3180.746 kb on - strand at 3180.750 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM remove 3,179,208 + +0.4 3,179,208 + -0.6 3,179,239 + -0.3 3,179,262 + -1.4 3,179,439 + BT2545 0.23 -1.3 3,179,474 + BT2545 0.28 -1.0 3,179,584 + BT2545 0.45 -2.2 3,179,585 - BT2545 0.45 -0.9 3,179,649 + BT2545 0.55 -0.7 3,179,745 + BT2545 0.70 -0.9 3,179,745 + BT2545 0.70 -0.8 3,179,821 + BT2545 0.81 +0.6 3,179,821 + BT2545 0.81 -0.3 3,179,823 + BT2545 0.82 +0.7 3,179,974 + -2.7 3,180,064 + BT2546 0.45 +0.1 3,180,148 + BT2546 0.84 -2.6 3,180,180 + -1.2 3,180,267 - -2.7 3,180,267 - -1.8 3,180,301 + +1.1 3,180,302 - -2.2 3,180,746 - +0.1 3,180,746 - -0.7 3,180,750 - -2.0
Or see this region's nucleotide sequence