Experiment: Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2172 and BT2173 are separated by 6 nucleotides BT2173 and BT2174 are separated by 28 nucleotides BT2174 and BT2175 overlap by 22 nucleotides
BT2172: BT2172 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 2,731,204 to 2,733,897
BT2172
BT2173: BT2173 - hypothetical protein (NCBI ptt file), at 2,733,904 to 2,734,917
BT2173
BT2174: BT2174 - ABC transporter ATP-binding protein (NCBI ptt file), at 2,734,946 to 2,735,623
BT2174
BT2175: BT2175 - L-fuculose kinase FucK (EC 2.7.1.51); D-ribulose 1-kinase (from data) , at 2,735,602 to 2,735,958
BT2175
Position (kb)
2733
2734
2735 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2732.928 kb on - strand, within BT2172 at 2732.963 kb on - strand at 2733.007 kb on - strand, within BT2172 at 2733.031 kb on + strand, within BT2172 at 2733.049 kb on + strand, within BT2172 at 2733.053 kb on + strand, within BT2172 at 2733.054 kb on - strand, within BT2172 at 2733.088 kb on - strand, within BT2172 at 2733.095 kb on + strand, within BT2172 at 2733.160 kb on + strand, within BT2172 at 2733.161 kb on - strand, within BT2172 at 2733.162 kb on + strand, within BT2172 at 2733.214 kb on + strand, within BT2172 at 2733.214 kb on + strand, within BT2172 at 2733.215 kb on - strand, within BT2172 at 2733.215 kb on - strand, within BT2172 at 2733.363 kb on + strand, within BT2172 at 2733.363 kb on + strand, within BT2172 at 2733.433 kb on + strand, within BT2172 at 2733.450 kb on + strand, within BT2172 at 2733.454 kb on - strand, within BT2172 at 2733.531 kb on + strand, within BT2172 at 2733.622 kb on - strand, within BT2172 at 2733.751 kb on - strand at 2733.778 kb on + strand at 2733.779 kb on - strand at 2733.816 kb on + strand at 2733.848 kb on - strand at 2733.877 kb on - strand at 2733.916 kb on - strand at 2733.916 kb on - strand at 2733.917 kb on + strand at 2733.919 kb on + strand at 2733.931 kb on - strand at 2733.939 kb on - strand at 2733.943 kb on - strand at 2733.943 kb on - strand at 2733.943 kb on - strand at 2733.943 kb on - strand at 2734.056 kb on - strand, within BT2173 at 2734.134 kb on - strand, within BT2173 at 2734.134 kb on - strand, within BT2173 at 2734.223 kb on + strand, within BT2173 at 2734.259 kb on - strand, within BT2173 at 2734.351 kb on - strand, within BT2173 at 2734.361 kb on - strand, within BT2173 at 2734.369 kb on + strand, within BT2173 at 2734.376 kb on + strand, within BT2173 at 2734.424 kb on + strand, within BT2173 at 2734.429 kb on - strand, within BT2173 at 2734.505 kb on - strand, within BT2173 at 2734.529 kb on + strand, within BT2173 at 2734.530 kb on - strand, within BT2173 at 2734.593 kb on + strand, within BT2173 at 2734.603 kb on + strand, within BT2173 at 2734.629 kb on + strand, within BT2173 at 2734.635 kb on + strand, within BT2173 at 2734.635 kb on + strand, within BT2173 at 2734.636 kb on - strand, within BT2173 at 2734.644 kb on + strand, within BT2173 at 2734.691 kb on - strand, within BT2173 at 2734.691 kb on - strand, within BT2173 at 2734.754 kb on - strand, within BT2173 at 2734.795 kb on + strand, within BT2173 at 2734.859 kb on + strand at 2734.860 kb on - strand at 2734.863 kb on + strand at 2734.864 kb on - strand at 2734.928 kb on + strand at 2734.932 kb on + strand at 2734.971 kb on + strand at 2735.068 kb on - strand, within BT2174 at 2735.117 kb on + strand, within BT2174 at 2735.132 kb on - strand, within BT2174 at 2735.155 kb on - strand, within BT2174 at 2735.209 kb on + strand, within BT2174 at 2735.210 kb on - strand, within BT2174 at 2735.312 kb on - strand, within BT2174 at 2735.331 kb on - strand, within BT2174 at 2735.364 kb on + strand, within BT2174 at 2735.387 kb on + strand, within BT2174 at 2735.425 kb on + strand, within BT2174 at 2735.425 kb on + strand, within BT2174 at 2735.426 kb on - strand, within BT2174 at 2735.528 kb on - strand, within BT2174 at 2735.540 kb on - strand, within BT2174 at 2735.596 kb on + strand at 2735.597 kb on - strand at 2735.597 kb on - strand at 2735.597 kb on - strand at 2735.641 kb on + strand, within BT2175 at 2735.652 kb on - strand, within BT2175 at 2735.653 kb on + strand, within BT2175 at 2735.654 kb on - strand, within BT2175 at 2735.714 kb on + strand, within BT2175 at 2735.715 kb on - strand, within BT2175 at 2735.739 kb on + strand, within BT2175 at 2735.741 kb on + strand, within BT2175 at 2735.776 kb on + strand, within BT2175 at 2735.836 kb on + strand, within BT2175 at 2735.836 kb on + strand, within BT2175 at 2735.837 kb on - strand, within BT2175 at 2735.837 kb on - strand, within BT2175 at 2735.837 kb on - strand, within BT2175 at 2735.841 kb on + strand, within BT2175 at 2735.907 kb on + strand, within BT2175 at 2735.914 kb on + strand, within BT2175 at 2735.914 kb on + strand, within BT2175 at 2735.914 kb on + strand, within BT2175
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM remove 2,732,928 - BT2172 0.64 +0.2 2,732,963 - -0.8 2,733,007 - BT2172 0.67 -3.6 2,733,031 + BT2172 0.68 +0.2 2,733,049 + BT2172 0.68 -2.3 2,733,053 + BT2172 0.69 -1.3 2,733,054 - BT2172 0.69 +0.5 2,733,088 - BT2172 0.70 -2.8 2,733,095 + BT2172 0.70 -0.8 2,733,160 + BT2172 0.73 -1.3 2,733,161 - BT2172 0.73 -3.4 2,733,162 + BT2172 0.73 +0.2 2,733,214 + BT2172 0.75 -2.5 2,733,214 + BT2172 0.75 -1.8 2,733,215 - BT2172 0.75 -1.8 2,733,215 - BT2172 0.75 -2.4 2,733,363 + BT2172 0.80 -1.4 2,733,363 + BT2172 0.80 -2.8 2,733,433 + BT2172 0.83 -3.1 2,733,450 + BT2172 0.83 -2.1 2,733,454 - BT2172 0.84 -1.8 2,733,531 + BT2172 0.86 -2.1 2,733,622 - BT2172 0.90 -2.1 2,733,751 - -2.1 2,733,778 + -0.8 2,733,779 - -3.1 2,733,816 + +0.2 2,733,848 - -1.4 2,733,877 - -2.5 2,733,916 - +0.2 2,733,916 - -1.5 2,733,917 + +0.2 2,733,919 + -1.0 2,733,931 - -0.8 2,733,939 - -2.1 2,733,943 - -3.3 2,733,943 - -1.4 2,733,943 - +0.2 2,733,943 - -0.6 2,734,056 - BT2173 0.15 -1.8 2,734,134 - BT2173 0.23 -2.8 2,734,134 - BT2173 0.23 -2.5 2,734,223 + BT2173 0.31 +0.2 2,734,259 - BT2173 0.35 -1.8 2,734,351 - BT2173 0.44 -5.3 2,734,361 - BT2173 0.45 -4.6 2,734,369 + BT2173 0.46 -3.0 2,734,376 + BT2173 0.47 -3.2 2,734,424 + BT2173 0.51 -1.4 2,734,429 - BT2173 0.52 -1.4 2,734,505 - BT2173 0.59 +0.2 2,734,529 + BT2173 0.62 -3.1 2,734,530 - BT2173 0.62 -0.7 2,734,593 + BT2173 0.68 -2.4 2,734,603 + BT2173 0.69 +0.2 2,734,629 + BT2173 0.71 +2.0 2,734,635 + BT2173 0.72 +0.9 2,734,635 + BT2173 0.72 -2.1 2,734,636 - BT2173 0.72 -1.8 2,734,644 + BT2173 0.73 -0.8 2,734,691 - BT2173 0.78 -0.8 2,734,691 - BT2173 0.78 +0.2 2,734,754 - BT2173 0.84 -3.6 2,734,795 + BT2173 0.88 -3.1 2,734,859 + +1.2 2,734,860 - -4.7 2,734,863 + -2.1 2,734,864 - -3.0 2,734,928 + -1.3 2,734,932 + +1.3 2,734,971 + -0.6 2,735,068 - BT2174 0.18 -0.8 2,735,117 + BT2174 0.25 -1.4 2,735,132 - BT2174 0.27 -0.2 2,735,155 - BT2174 0.31 -2.6 2,735,209 + BT2174 0.39 -0.8 2,735,210 - BT2174 0.39 -1.9 2,735,312 - BT2174 0.54 +2.8 2,735,331 - BT2174 0.57 -2.4 2,735,364 + BT2174 0.62 -3.4 2,735,387 + BT2174 0.65 -1.8 2,735,425 + BT2174 0.71 -2.1 2,735,425 + BT2174 0.71 +0.2 2,735,426 - BT2174 0.71 -2.1 2,735,528 - BT2174 0.86 -0.7 2,735,540 - BT2174 0.88 -2.1 2,735,596 + -0.8 2,735,597 - +0.2 2,735,597 - -2.6 2,735,597 - -0.3 2,735,641 + BT2175 0.11 +0.2 2,735,652 - BT2175 0.14 -0.7 2,735,653 + BT2175 0.14 -2.1 2,735,654 - BT2175 0.15 -2.6 2,735,714 + BT2175 0.31 +0.2 2,735,715 - BT2175 0.32 -0.6 2,735,739 + BT2175 0.38 +0.2 2,735,741 + BT2175 0.39 -2.1 2,735,776 + BT2175 0.49 -0.8 2,735,836 + BT2175 0.66 +0.2 2,735,836 + BT2175 0.66 +0.2 2,735,837 - BT2175 0.66 -1.4 2,735,837 - BT2175 0.66 -0.7 2,735,837 - BT2175 0.66 -1.2 2,735,841 + BT2175 0.67 -0.2 2,735,907 + BT2175 0.85 -1.4 2,735,914 + BT2175 0.87 -2.1 2,735,914 + BT2175 0.87 -0.9 2,735,914 + BT2175 0.87 -2.1
Or see this region's nucleotide sequence