Experiment: Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2073 and BT2074 are separated by 532 nucleotides BT2074 and BT2075 are separated by 28 nucleotides BT2075 and BT2076 overlap by 1 nucleotides
BT2073: BT2073 - putative helicase (NCBI ptt file), at 2,596,590 to 2,598,548
BT2073
BT2074: BT2074 - ketol-acid reductoisomerase (NCBI ptt file), at 2,599,081 to 2,600,160
BT2074
BT2075: BT2075 - acyl-ACP thioesterase (NCBI ptt file), at 2,600,189 to 2,600,932
BT2075
BT2076: BT2076 - acetohydroxybutanoate synthase regulatory subunit (EC 2.2.1.6) (from data) , at 2,600,932 to 2,601,495
BT2076
Position (kb)
2599
2600
2601 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2598.171 kb on + strand, within BT2073 at 2598.172 kb on - strand, within BT2073 at 2598.172 kb on - strand, within BT2073 at 2598.174 kb on - strand, within BT2073 at 2598.174 kb on - strand, within BT2073 at 2598.256 kb on - strand, within BT2073 at 2598.256 kb on - strand, within BT2073 at 2598.266 kb on + strand, within BT2073 at 2598.415 kb on + strand at 2598.415 kb on + strand at 2598.519 kb on + strand at 2598.520 kb on - strand at 2598.602 kb on - strand at 2598.633 kb on + strand at 2598.678 kb on - strand at 2598.712 kb on + strand at 2598.727 kb on - strand at 2598.763 kb on - strand at 2598.763 kb on - strand at 2598.805 kb on - strand at 2598.825 kb on + strand at 2598.852 kb on + strand at 2598.857 kb on - strand at 2598.927 kb on + strand at 2598.928 kb on - strand at 2598.928 kb on - strand at 2598.928 kb on - strand at 2598.948 kb on - strand at 2598.948 kb on - strand at 2598.948 kb on - strand at 2598.997 kb on - strand at 2599.015 kb on + strand at 2599.019 kb on + strand at 2599.065 kb on + strand at 2599.067 kb on + strand at 2599.068 kb on - strand at 2599.068 kb on - strand at 2599.071 kb on - strand at 2599.083 kb on - strand at 2599.087 kb on - strand at 2599.267 kb on - strand, within BT2074 at 2599.326 kb on + strand, within BT2074 at 2599.331 kb on + strand, within BT2074 at 2599.331 kb on + strand, within BT2074 at 2599.332 kb on - strand, within BT2074 at 2599.332 kb on - strand, within BT2074 at 2599.332 kb on - strand, within BT2074 at 2599.390 kb on + strand, within BT2074 at 2599.445 kb on + strand, within BT2074 at 2599.470 kb on + strand, within BT2074 at 2599.471 kb on - strand, within BT2074 at 2599.610 kb on + strand, within BT2074 at 2599.653 kb on + strand, within BT2074 at 2599.656 kb on + strand, within BT2074 at 2599.657 kb on - strand, within BT2074 at 2599.669 kb on - strand, within BT2074 at 2599.696 kb on + strand, within BT2074 at 2599.696 kb on + strand, within BT2074 at 2599.697 kb on - strand, within BT2074 at 2599.697 kb on - strand, within BT2074 at 2599.721 kb on - strand, within BT2074 at 2599.801 kb on - strand, within BT2074 at 2599.820 kb on - strand, within BT2074 at 2599.845 kb on + strand, within BT2074 at 2599.928 kb on + strand, within BT2074 at 2599.957 kb on + strand, within BT2074 at 2599.958 kb on - strand, within BT2074 at 2599.958 kb on - strand, within BT2074 at 2600.087 kb on - strand at 2600.087 kb on - strand at 2600.165 kb on + strand at 2600.181 kb on - strand at 2600.186 kb on - strand at 2600.187 kb on + strand at 2600.188 kb on - strand at 2600.216 kb on + strand at 2600.216 kb on + strand at 2600.216 kb on + strand at 2600.220 kb on - strand at 2600.247 kb on - strand at 2600.319 kb on + strand, within BT2075 at 2600.319 kb on + strand, within BT2075 at 2600.319 kb on + strand, within BT2075 at 2600.320 kb on - strand, within BT2075 at 2600.342 kb on - strand, within BT2075 at 2600.356 kb on - strand, within BT2075 at 2600.381 kb on + strand, within BT2075 at 2600.520 kb on - strand, within BT2075 at 2600.521 kb on + strand, within BT2075 at 2600.521 kb on + strand, within BT2075 at 2600.522 kb on - strand, within BT2075 at 2600.524 kb on - strand, within BT2075 at 2600.710 kb on - strand, within BT2075 at 2600.763 kb on + strand, within BT2075 at 2600.764 kb on - strand, within BT2075 at 2600.782 kb on + strand, within BT2075 at 2600.782 kb on + strand, within BT2075 at 2600.782 kb on + strand, within BT2075 at 2600.783 kb on - strand, within BT2075 at 2600.904 kb on + strand at 2600.934 kb on - strand at 2601.015 kb on + strand, within BT2076 at 2601.015 kb on + strand, within BT2076 at 2601.015 kb on + strand, within BT2076 at 2601.087 kb on + strand, within BT2076 at 2601.143 kb on - strand, within BT2076 at 2601.156 kb on + strand, within BT2076 at 2601.158 kb on + strand, within BT2076 at 2601.159 kb on - strand, within BT2076
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM remove 2,598,171 + BT2073 0.81 +0.2 2,598,172 - BT2073 0.81 -1.8 2,598,172 - BT2073 0.81 +0.5 2,598,174 - BT2073 0.81 -0.6 2,598,174 - BT2073 0.81 -3.1 2,598,256 - BT2073 0.85 -0.3 2,598,256 - BT2073 0.85 +1.8 2,598,266 + BT2073 0.86 -1.3 2,598,415 + -1.9 2,598,415 + -0.8 2,598,519 + -1.4 2,598,520 - -1.2 2,598,602 - -0.1 2,598,633 + -2.6 2,598,678 - +0.2 2,598,712 + -2.0 2,598,727 - +0.5 2,598,763 - -2.4 2,598,763 - +0.6 2,598,805 - -0.8 2,598,825 + +0.2 2,598,852 + +0.2 2,598,857 - -1.5 2,598,927 + +0.2 2,598,928 - -1.6 2,598,928 - +0.2 2,598,928 - -3.0 2,598,948 - -2.6 2,598,948 - -1.8 2,598,948 - +1.2 2,598,997 - -0.9 2,599,015 + -2.1 2,599,019 + -2.3 2,599,065 + -4.1 2,599,067 + -3.0 2,599,068 - -2.6 2,599,068 - -3.0 2,599,071 - -0.8 2,599,083 - -1.8 2,599,087 - -2.5 2,599,267 - BT2074 0.17 -4.2 2,599,326 + BT2074 0.23 -3.4 2,599,331 + BT2074 0.23 -0.8 2,599,331 + BT2074 0.23 +0.2 2,599,332 - BT2074 0.23 -2.1 2,599,332 - BT2074 0.23 -2.1 2,599,332 - BT2074 0.23 -3.7 2,599,390 + BT2074 0.29 +0.2 2,599,445 + BT2074 0.34 -2.1 2,599,470 + BT2074 0.36 -3.1 2,599,471 - BT2074 0.36 -1.8 2,599,610 + BT2074 0.49 +0.2 2,599,653 + BT2074 0.53 -4.2 2,599,656 + BT2074 0.53 -5.0 2,599,657 - BT2074 0.53 -5.6 2,599,669 - BT2074 0.54 -2.8 2,599,696 + BT2074 0.57 -4.4 2,599,696 + BT2074 0.57 +0.2 2,599,697 - BT2074 0.57 -4.3 2,599,697 - BT2074 0.57 -0.8 2,599,721 - BT2074 0.59 -3.9 2,599,801 - BT2074 0.67 -3.1 2,599,820 - BT2074 0.68 -0.8 2,599,845 + BT2074 0.71 -2.4 2,599,928 + BT2074 0.78 +0.2 2,599,957 + BT2074 0.81 -3.4 2,599,958 - BT2074 0.81 -0.8 2,599,958 - BT2074 0.81 +0.2 2,600,087 - +0.2 2,600,087 - -2.6 2,600,165 + -1.4 2,600,181 - -0.8 2,600,186 - -2.0 2,600,187 + -3.4 2,600,188 - -0.8 2,600,216 + -1.8 2,600,216 + -2.7 2,600,216 + -0.8 2,600,220 - -1.0 2,600,247 - -3.8 2,600,319 + BT2075 0.17 -1.8 2,600,319 + BT2075 0.17 -5.1 2,600,319 + BT2075 0.17 -0.8 2,600,320 - BT2075 0.18 +0.1 2,600,342 - BT2075 0.21 -1.5 2,600,356 - BT2075 0.22 -0.2 2,600,381 + BT2075 0.26 -3.9 2,600,520 - BT2075 0.44 -1.4 2,600,521 + BT2075 0.45 -1.4 2,600,521 + BT2075 0.45 -4.4 2,600,522 - BT2075 0.45 -1.8 2,600,524 - BT2075 0.45 +0.8 2,600,710 - BT2075 0.70 -0.4 2,600,763 + BT2075 0.77 -5.4 2,600,764 - BT2075 0.77 +1.2 2,600,782 + BT2075 0.80 -2.1 2,600,782 + BT2075 0.80 -1.8 2,600,782 + BT2075 0.80 -1.5 2,600,783 - BT2075 0.80 +2.8 2,600,904 + -1.4 2,600,934 - -1.4 2,601,015 + BT2076 0.15 +1.0 2,601,015 + BT2076 0.15 -3.5 2,601,015 + BT2076 0.15 -2.8 2,601,087 + BT2076 0.27 -2.1 2,601,143 - BT2076 0.37 -1.8 2,601,156 + BT2076 0.40 -2.8 2,601,158 + BT2076 0.40 -2.4 2,601,159 - BT2076 0.40 -3.8
Or see this region's nucleotide sequence