Experiment: Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2040 and BT2041 are separated by 82 nucleotides BT2041 and BT2042 are separated by 74 nucleotides BT2042 and BT2043 are separated by 108 nucleotides BT2043 and BT2044 overlap by 4 nucleotides
BT2040: BT2040 - hypothetical protein (NCBI ptt file), at 2,561,968 to 2,563,161
BT2040
BT2041: BT2041 - hypothetical protein (NCBI ptt file), at 2,563,244 to 2,564,068
BT2041
BT2042: BT2042 - tRNA and rRNA cytosine-C5-methylase (NCBI ptt file), at 2,564,143 to 2,565,621
BT2042
BT2043: BT2043 - hypothetical protein (NCBI ptt file), at 2,565,730 to 2,566,482
BT2043
BT2044: BT2044 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 2,566,479 to 2,567,027
BT2044
Position (kb)
2564
2565
2566 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2563.158 kb on - strand at 2563.173 kb on - strand at 2563.210 kb on + strand at 2563.225 kb on + strand at 2563.240 kb on + strand at 2563.240 kb on + strand at 2563.240 kb on + strand at 2563.352 kb on + strand, within BT2041 at 2563.379 kb on + strand, within BT2041 at 2563.494 kb on - strand, within BT2041 at 2563.587 kb on - strand, within BT2041 at 2563.589 kb on + strand, within BT2041 at 2563.608 kb on + strand, within BT2041 at 2563.770 kb on - strand, within BT2041 at 2563.770 kb on - strand, within BT2041 at 2563.770 kb on - strand, within BT2041 at 2563.813 kb on + strand, within BT2041 at 2563.902 kb on + strand, within BT2041 at 2563.902 kb on + strand, within BT2041 at 2563.903 kb on - strand, within BT2041 at 2563.968 kb on - strand, within BT2041 at 2564.036 kb on + strand at 2564.046 kb on + strand at 2564.047 kb on - strand at 2564.272 kb on - strand at 2564.301 kb on + strand, within BT2042 at 2564.302 kb on - strand, within BT2042 at 2564.383 kb on - strand, within BT2042 at 2564.497 kb on - strand, within BT2042 at 2564.673 kb on - strand, within BT2042 at 2564.902 kb on - strand, within BT2042 at 2565.507 kb on + strand at 2565.593 kb on + strand at 2565.684 kb on - strand at 2565.697 kb on - strand at 2565.752 kb on + strand at 2565.805 kb on - strand at 2565.939 kb on - strand, within BT2043 at 2565.939 kb on - strand, within BT2043 at 2566.056 kb on + strand, within BT2043 at 2566.264 kb on - strand, within BT2043 at 2566.477 kb on - strand at 2566.496 kb on + strand at 2566.497 kb on - strand at 2566.497 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM remove 2,563,158 - -1.1 2,563,173 - -1.3 2,563,210 + -0.7 2,563,225 + -0.7 2,563,240 + -1.3 2,563,240 + -1.4 2,563,240 + -0.8 2,563,352 + BT2041 0.13 +0.7 2,563,379 + BT2041 0.16 +0.2 2,563,494 - BT2041 0.30 -1.8 2,563,587 - BT2041 0.42 +0.2 2,563,589 + BT2041 0.42 -3.2 2,563,608 + BT2041 0.44 -3.2 2,563,770 - BT2041 0.64 -0.8 2,563,770 - BT2041 0.64 +0.0 2,563,770 - BT2041 0.64 -0.8 2,563,813 + BT2041 0.69 -1.0 2,563,902 + BT2041 0.80 -2.4 2,563,902 + BT2041 0.80 -0.6 2,563,903 - BT2041 0.80 -0.8 2,563,968 - BT2041 0.88 -0.3 2,564,036 + -1.8 2,564,046 + -1.4 2,564,047 - -0.8 2,564,272 - -1.4 2,564,301 + BT2042 0.11 -2.2 2,564,302 - BT2042 0.11 -0.8 2,564,383 - BT2042 0.16 -1.4 2,564,497 - BT2042 0.24 +1.7 2,564,673 - BT2042 0.36 -1.4 2,564,902 - BT2042 0.51 -2.8 2,565,507 + +0.4 2,565,593 + -3.3 2,565,684 - +0.1 2,565,697 - -0.8 2,565,752 + -1.1 2,565,805 - -1.8 2,565,939 - BT2043 0.28 -0.8 2,565,939 - BT2043 0.28 -3.4 2,566,056 + BT2043 0.43 -2.1 2,566,264 - BT2043 0.71 -1.4 2,566,477 - -2.2 2,566,496 + -1.4 2,566,497 - -1.3 2,566,497 - -0.8
Or see this region's nucleotide sequence