Experiment: Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1860 and BT1861 are separated by 77 nucleotides BT1861 and BT1862 are separated by 397 nucleotides BT1862 and BT_r04 are separated by 175 nucleotides
BT1860: BT1860 - 3-isopropylmalate dehydratase large subunit (NCBI ptt file), at 2,333,231 to 2,334,625
BT1860
BT1861: BT1861 - 2-isopropylmalate synthase (EC 2.3.3.13) (from data) , at 2,334,703 to 2,336,199
BT1861
BT1862: BT1862 - hypothetical protein (NCBI ptt file), at 2,336,597 to 2,336,839
BT1862
BT_r04: BT_r04 - 5S ribosomal RNA (RefSeq), at 2,337,015 to 2,337,048
_r04
Position (kb)
2334
2335
2336
2337 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2333.711 kb on - strand, within BT1860 at 2333.714 kb on - strand, within BT1860 at 2333.765 kb on - strand, within BT1860 at 2333.936 kb on - strand, within BT1860 at 2333.968 kb on + strand, within BT1860 at 2333.978 kb on - strand, within BT1860 at 2334.021 kb on + strand, within BT1860 at 2334.124 kb on + strand, within BT1860 at 2334.158 kb on - strand, within BT1860 at 2334.376 kb on + strand, within BT1860 at 2334.436 kb on + strand, within BT1860 at 2334.551 kb on - strand at 2334.552 kb on + strand at 2334.553 kb on - strand at 2334.630 kb on + strand at 2334.631 kb on - strand at 2334.631 kb on - strand at 2334.646 kb on - strand at 2334.650 kb on - strand at 2334.673 kb on - strand at 2334.734 kb on + strand at 2334.735 kb on - strand at 2334.742 kb on + strand at 2334.756 kb on + strand at 2334.765 kb on + strand at 2334.765 kb on + strand at 2334.765 kb on + strand at 2334.813 kb on + strand at 2334.814 kb on - strand at 2334.823 kb on - strand at 2334.934 kb on - strand, within BT1861 at 2334.969 kb on + strand, within BT1861 at 2335.038 kb on + strand, within BT1861 at 2335.039 kb on - strand, within BT1861 at 2335.073 kb on - strand, within BT1861 at 2335.078 kb on + strand, within BT1861 at 2335.078 kb on + strand, within BT1861 at 2335.096 kb on - strand, within BT1861 at 2335.135 kb on + strand, within BT1861 at 2335.136 kb on - strand, within BT1861 at 2335.136 kb on - strand, within BT1861 at 2335.246 kb on + strand, within BT1861 at 2335.246 kb on + strand, within BT1861 at 2335.247 kb on - strand, within BT1861 at 2335.280 kb on - strand, within BT1861 at 2335.368 kb on + strand, within BT1861 at 2335.368 kb on + strand, within BT1861 at 2335.561 kb on - strand, within BT1861 at 2335.588 kb on - strand, within BT1861 at 2335.690 kb on - strand, within BT1861 at 2335.698 kb on + strand, within BT1861 at 2335.698 kb on + strand, within BT1861 at 2335.698 kb on + strand, within BT1861 at 2335.698 kb on + strand, within BT1861 at 2335.751 kb on + strand, within BT1861 at 2335.752 kb on - strand, within BT1861 at 2335.817 kb on + strand, within BT1861 at 2335.878 kb on + strand, within BT1861 at 2335.879 kb on - strand, within BT1861 at 2335.908 kb on + strand, within BT1861 at 2335.914 kb on + strand, within BT1861 at 2335.914 kb on + strand, within BT1861 at 2335.914 kb on + strand, within BT1861 at 2335.960 kb on - strand, within BT1861 at 2336.034 kb on + strand, within BT1861 at 2336.181 kb on + strand at 2336.217 kb on + strand at 2336.284 kb on - strand at 2336.327 kb on - strand at 2336.334 kb on + strand at 2336.334 kb on + strand at 2336.344 kb on + strand at 2336.345 kb on - strand at 2336.348 kb on - strand at 2336.348 kb on - strand at 2336.352 kb on + strand at 2336.352 kb on + strand at 2336.353 kb on - strand at 2336.356 kb on + strand at 2336.357 kb on - strand at 2336.358 kb on + strand at 2336.390 kb on - strand at 2336.416 kb on - strand at 2336.519 kb on + strand at 2336.578 kb on - strand at 2336.578 kb on - strand at 2336.586 kb on - strand at 2336.598 kb on + strand at 2336.714 kb on + strand, within BT1862 at 2336.714 kb on + strand, within BT1862 at 2336.758 kb on + strand, within BT1862 at 2336.759 kb on - strand, within BT1862 at 2336.759 kb on - strand, within BT1862 at 2336.762 kb on - strand, within BT1862 at 2336.811 kb on - strand, within BT1862 at 2336.811 kb on - strand, within BT1862 at 2336.840 kb on - strand at 2336.847 kb on - strand at 2336.890 kb on - strand at 2336.891 kb on + strand at 2336.892 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM remove 2,333,711 - BT1860 0.34 -2.6 2,333,714 - BT1860 0.35 -1.8 2,333,765 - BT1860 0.38 -3.6 2,333,936 - BT1860 0.51 -2.8 2,333,968 + BT1860 0.53 -0.8 2,333,978 - BT1860 0.54 -0.8 2,334,021 + BT1860 0.57 -3.6 2,334,124 + BT1860 0.64 -4.9 2,334,158 - BT1860 0.66 -4.0 2,334,376 + BT1860 0.82 -3.7 2,334,436 + BT1860 0.86 -1.2 2,334,551 - -1.4 2,334,552 + -3.1 2,334,553 - -2.6 2,334,630 + -1.4 2,334,631 - -0.8 2,334,631 - -1.4 2,334,646 - +0.2 2,334,650 - -1.4 2,334,673 - -2.3 2,334,734 + -1.4 2,334,735 - -0.8 2,334,742 + -0.8 2,334,756 + +0.2 2,334,765 + -2.8 2,334,765 + -1.8 2,334,765 + -1.8 2,334,813 + -1.4 2,334,814 - -0.8 2,334,823 - -0.8 2,334,934 - BT1861 0.15 -1.4 2,334,969 + BT1861 0.18 -2.4 2,335,038 + BT1861 0.22 -3.8 2,335,039 - BT1861 0.22 -0.4 2,335,073 - BT1861 0.25 -3.0 2,335,078 + BT1861 0.25 -3.1 2,335,078 + BT1861 0.25 -0.9 2,335,096 - BT1861 0.26 -2.4 2,335,135 + BT1861 0.29 +0.2 2,335,136 - BT1861 0.29 -2.4 2,335,136 - BT1861 0.29 -1.8 2,335,246 + BT1861 0.36 +1.2 2,335,246 + BT1861 0.36 +0.2 2,335,247 - BT1861 0.36 +0.2 2,335,280 - BT1861 0.39 -1.8 2,335,368 + BT1861 0.44 -3.6 2,335,368 + BT1861 0.44 -4.4 2,335,561 - BT1861 0.57 -1.4 2,335,588 - BT1861 0.59 -1.8 2,335,690 - BT1861 0.66 -0.8 2,335,698 + BT1861 0.66 -2.6 2,335,698 + BT1861 0.66 -1.4 2,335,698 + BT1861 0.66 -0.8 2,335,698 + BT1861 0.66 -0.8 2,335,751 + BT1861 0.70 -5.1 2,335,752 - BT1861 0.70 -2.1 2,335,817 + BT1861 0.74 -0.8 2,335,878 + BT1861 0.78 -1.8 2,335,879 - BT1861 0.79 -1.4 2,335,908 + BT1861 0.80 -4.7 2,335,914 + BT1861 0.81 -1.4 2,335,914 + BT1861 0.81 -3.2 2,335,914 + BT1861 0.81 +0.2 2,335,960 - BT1861 0.84 -3.4 2,336,034 + BT1861 0.89 -4.3 2,336,181 + -6.4 2,336,217 + -3.1 2,336,284 - -4.0 2,336,327 - -0.8 2,336,334 + -2.6 2,336,334 + -1.8 2,336,344 + +0.2 2,336,345 - +0.5 2,336,348 - -0.5 2,336,348 - -1.5 2,336,352 + -0.8 2,336,352 + -3.7 2,336,353 - -2.7 2,336,356 + -3.4 2,336,357 - -0.8 2,336,358 + -4.4 2,336,390 - +1.2 2,336,416 - +0.2 2,336,519 + +0.2 2,336,578 - -0.9 2,336,578 - -0.2 2,336,586 - -2.1 2,336,598 + -1.4 2,336,714 + BT1862 0.48 +0.2 2,336,714 + BT1862 0.48 -3.0 2,336,758 + BT1862 0.66 -0.6 2,336,759 - BT1862 0.67 -1.6 2,336,759 - BT1862 0.67 -0.2 2,336,762 - BT1862 0.68 -0.8 2,336,811 - BT1862 0.88 -2.6 2,336,811 - BT1862 0.88 -0.5 2,336,840 - -0.7 2,336,847 - -1.8 2,336,890 - -0.2 2,336,891 + -0.2 2,336,892 - -2.1
Or see this region's nucleotide sequence