Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT1861

Experiment: Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT1860 and BT1861 are separated by 77 nucleotidesBT1861 and BT1862 are separated by 397 nucleotidesBT1862 and BT_r04 are separated by 175 nucleotides BT1860: BT1860 - 3-isopropylmalate dehydratase large subunit (NCBI ptt file), at 2,333,231 to 2,334,625 BT1860 BT1861: BT1861 - 2-isopropylmalate synthase (EC 2.3.3.13) (from data), at 2,334,703 to 2,336,199 BT1861 BT1862: BT1862 - hypothetical protein (NCBI ptt file), at 2,336,597 to 2,336,839 BT1862 BT_r04: BT_r04 - 5S ribosomal RNA (RefSeq), at 2,337,015 to 2,337,048 _r04 Position (kb) 2334 2335 2336 2337Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 2333.711 kb on - strand, within BT1860at 2333.714 kb on - strand, within BT1860at 2333.765 kb on - strand, within BT1860at 2333.936 kb on - strand, within BT1860at 2333.968 kb on + strand, within BT1860at 2333.978 kb on - strand, within BT1860at 2334.021 kb on + strand, within BT1860at 2334.124 kb on + strand, within BT1860at 2334.158 kb on - strand, within BT1860at 2334.376 kb on + strand, within BT1860at 2334.436 kb on + strand, within BT1860at 2334.551 kb on - strandat 2334.552 kb on + strandat 2334.553 kb on - strandat 2334.630 kb on + strandat 2334.631 kb on - strandat 2334.631 kb on - strandat 2334.646 kb on - strandat 2334.650 kb on - strandat 2334.673 kb on - strandat 2334.734 kb on + strandat 2334.735 kb on - strandat 2334.742 kb on + strandat 2334.756 kb on + strandat 2334.765 kb on + strandat 2334.765 kb on + strandat 2334.765 kb on + strandat 2334.813 kb on + strandat 2334.814 kb on - strandat 2334.823 kb on - strandat 2334.934 kb on - strand, within BT1861at 2334.969 kb on + strand, within BT1861at 2335.038 kb on + strand, within BT1861at 2335.039 kb on - strand, within BT1861at 2335.073 kb on - strand, within BT1861at 2335.078 kb on + strand, within BT1861at 2335.078 kb on + strand, within BT1861at 2335.096 kb on - strand, within BT1861at 2335.135 kb on + strand, within BT1861at 2335.136 kb on - strand, within BT1861at 2335.136 kb on - strand, within BT1861at 2335.246 kb on + strand, within BT1861at 2335.246 kb on + strand, within BT1861at 2335.247 kb on - strand, within BT1861at 2335.280 kb on - strand, within BT1861at 2335.368 kb on + strand, within BT1861at 2335.368 kb on + strand, within BT1861at 2335.561 kb on - strand, within BT1861at 2335.588 kb on - strand, within BT1861at 2335.690 kb on - strand, within BT1861at 2335.698 kb on + strand, within BT1861at 2335.698 kb on + strand, within BT1861at 2335.698 kb on + strand, within BT1861at 2335.698 kb on + strand, within BT1861at 2335.751 kb on + strand, within BT1861at 2335.752 kb on - strand, within BT1861at 2335.817 kb on + strand, within BT1861at 2335.878 kb on + strand, within BT1861at 2335.879 kb on - strand, within BT1861at 2335.908 kb on + strand, within BT1861at 2335.914 kb on + strand, within BT1861at 2335.914 kb on + strand, within BT1861at 2335.914 kb on + strand, within BT1861at 2335.960 kb on - strand, within BT1861at 2336.034 kb on + strand, within BT1861at 2336.181 kb on + strandat 2336.217 kb on + strandat 2336.284 kb on - strandat 2336.327 kb on - strandat 2336.334 kb on + strandat 2336.334 kb on + strandat 2336.344 kb on + strandat 2336.345 kb on - strandat 2336.348 kb on - strandat 2336.348 kb on - strandat 2336.352 kb on + strandat 2336.352 kb on + strandat 2336.353 kb on - strandat 2336.356 kb on + strandat 2336.357 kb on - strandat 2336.358 kb on + strandat 2336.390 kb on - strandat 2336.416 kb on - strandat 2336.519 kb on + strandat 2336.578 kb on - strandat 2336.578 kb on - strandat 2336.586 kb on - strandat 2336.598 kb on + strandat 2336.714 kb on + strand, within BT1862at 2336.714 kb on + strand, within BT1862at 2336.758 kb on + strand, within BT1862at 2336.759 kb on - strand, within BT1862at 2336.759 kb on - strand, within BT1862at 2336.762 kb on - strand, within BT1862at 2336.811 kb on - strand, within BT1862at 2336.811 kb on - strand, within BT1862at 2336.840 kb on - strandat 2336.847 kb on - strandat 2336.890 kb on - strandat 2336.891 kb on + strandat 2336.892 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM
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2,333,711 - BT1860 0.34 -2.6
2,333,714 - BT1860 0.35 -1.8
2,333,765 - BT1860 0.38 -3.6
2,333,936 - BT1860 0.51 -2.8
2,333,968 + BT1860 0.53 -0.8
2,333,978 - BT1860 0.54 -0.8
2,334,021 + BT1860 0.57 -3.6
2,334,124 + BT1860 0.64 -4.9
2,334,158 - BT1860 0.66 -4.0
2,334,376 + BT1860 0.82 -3.7
2,334,436 + BT1860 0.86 -1.2
2,334,551 - -1.4
2,334,552 + -3.1
2,334,553 - -2.6
2,334,630 + -1.4
2,334,631 - -0.8
2,334,631 - -1.4
2,334,646 - +0.2
2,334,650 - -1.4
2,334,673 - -2.3
2,334,734 + -1.4
2,334,735 - -0.8
2,334,742 + -0.8
2,334,756 + +0.2
2,334,765 + -2.8
2,334,765 + -1.8
2,334,765 + -1.8
2,334,813 + -1.4
2,334,814 - -0.8
2,334,823 - -0.8
2,334,934 - BT1861 0.15 -1.4
2,334,969 + BT1861 0.18 -2.4
2,335,038 + BT1861 0.22 -3.8
2,335,039 - BT1861 0.22 -0.4
2,335,073 - BT1861 0.25 -3.0
2,335,078 + BT1861 0.25 -3.1
2,335,078 + BT1861 0.25 -0.9
2,335,096 - BT1861 0.26 -2.4
2,335,135 + BT1861 0.29 +0.2
2,335,136 - BT1861 0.29 -2.4
2,335,136 - BT1861 0.29 -1.8
2,335,246 + BT1861 0.36 +1.2
2,335,246 + BT1861 0.36 +0.2
2,335,247 - BT1861 0.36 +0.2
2,335,280 - BT1861 0.39 -1.8
2,335,368 + BT1861 0.44 -3.6
2,335,368 + BT1861 0.44 -4.4
2,335,561 - BT1861 0.57 -1.4
2,335,588 - BT1861 0.59 -1.8
2,335,690 - BT1861 0.66 -0.8
2,335,698 + BT1861 0.66 -2.6
2,335,698 + BT1861 0.66 -1.4
2,335,698 + BT1861 0.66 -0.8
2,335,698 + BT1861 0.66 -0.8
2,335,751 + BT1861 0.70 -5.1
2,335,752 - BT1861 0.70 -2.1
2,335,817 + BT1861 0.74 -0.8
2,335,878 + BT1861 0.78 -1.8
2,335,879 - BT1861 0.79 -1.4
2,335,908 + BT1861 0.80 -4.7
2,335,914 + BT1861 0.81 -1.4
2,335,914 + BT1861 0.81 -3.2
2,335,914 + BT1861 0.81 +0.2
2,335,960 - BT1861 0.84 -3.4
2,336,034 + BT1861 0.89 -4.3
2,336,181 + -6.4
2,336,217 + -3.1
2,336,284 - -4.0
2,336,327 - -0.8
2,336,334 + -2.6
2,336,334 + -1.8
2,336,344 + +0.2
2,336,345 - +0.5
2,336,348 - -0.5
2,336,348 - -1.5
2,336,352 + -0.8
2,336,352 + -3.7
2,336,353 - -2.7
2,336,356 + -3.4
2,336,357 - -0.8
2,336,358 + -4.4
2,336,390 - +1.2
2,336,416 - +0.2
2,336,519 + +0.2
2,336,578 - -0.9
2,336,578 - -0.2
2,336,586 - -2.1
2,336,598 + -1.4
2,336,714 + BT1862 0.48 +0.2
2,336,714 + BT1862 0.48 -3.0
2,336,758 + BT1862 0.66 -0.6
2,336,759 - BT1862 0.67 -1.6
2,336,759 - BT1862 0.67 -0.2
2,336,762 - BT1862 0.68 -0.8
2,336,811 - BT1862 0.88 -2.6
2,336,811 - BT1862 0.88 -0.5
2,336,840 - -0.7
2,336,847 - -1.8
2,336,890 - -0.2
2,336,891 + -0.2
2,336,892 - -2.1

Or see this region's nucleotide sequence