Experiment: Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1857 and BT1858 are separated by 90 nucleotides BT1858 and BT1859 are separated by 5 nucleotides BT1859 and BT1860 are separated by 42 nucleotides
BT1857: BT1857 - 3-isopropylmalate dehydrogenase (NCBI ptt file), at 2,329,929 to 2,330,990
BT1857
BT1858: BT1858 - (R)-citramalate synthase (EC 2.3.1.182) (from data) , at 2,331,081 to 2,332,580
BT1858
BT1859: BT1859 - 3-isopropylmalate dehydratase small subunit (NCBI ptt file), at 2,332,586 to 2,333,188
BT1859
BT1860: BT1860 - 3-isopropylmalate dehydratase large subunit (NCBI ptt file), at 2,333,231 to 2,334,625
BT1860
Position (kb)
2331
2332
2333 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 2330.100 kb on - strand, within BT1857 at 2330.100 kb on - strand, within BT1857 at 2330.104 kb on - strand, within BT1857 at 2330.181 kb on + strand, within BT1857 at 2330.189 kb on + strand, within BT1857 at 2330.201 kb on + strand, within BT1857 at 2330.273 kb on + strand, within BT1857 at 2330.345 kb on + strand, within BT1857 at 2330.346 kb on - strand, within BT1857 at 2330.348 kb on - strand, within BT1857 at 2330.471 kb on - strand, within BT1857 at 2330.519 kb on - strand, within BT1857 at 2330.531 kb on - strand, within BT1857 at 2330.538 kb on - strand, within BT1857 at 2330.544 kb on - strand at 2330.640 kb on - strand, within BT1857 at 2330.640 kb on - strand, within BT1857 at 2330.769 kb on + strand, within BT1857 at 2330.812 kb on + strand, within BT1857 at 2330.813 kb on - strand, within BT1857 at 2330.920 kb on - strand at 2330.920 kb on - strand at 2330.926 kb on - strand at 2330.982 kb on - strand at 2331.031 kb on + strand at 2331.032 kb on - strand at 2331.044 kb on - strand at 2331.067 kb on - strand at 2331.179 kb on - strand at 2331.192 kb on + strand at 2331.193 kb on - strand at 2331.193 kb on - strand at 2331.193 kb on - strand at 2331.193 kb on - strand at 2331.215 kb on + strand at 2331.234 kb on - strand, within BT1858 at 2331.267 kb on - strand, within BT1858 at 2331.274 kb on - strand, within BT1858 at 2331.319 kb on + strand, within BT1858 at 2331.319 kb on + strand, within BT1858 at 2331.319 kb on + strand, within BT1858 at 2331.364 kb on - strand, within BT1858 at 2331.463 kb on - strand, within BT1858 at 2331.476 kb on + strand, within BT1858 at 2331.476 kb on + strand, within BT1858 at 2331.477 kb on - strand, within BT1858 at 2331.593 kb on + strand, within BT1858 at 2331.599 kb on + strand, within BT1858 at 2331.599 kb on + strand, within BT1858 at 2331.631 kb on + strand, within BT1858 at 2331.825 kb on - strand, within BT1858 at 2332.039 kb on + strand, within BT1858 at 2332.040 kb on - strand, within BT1858 at 2332.044 kb on + strand, within BT1858 at 2332.088 kb on + strand, within BT1858 at 2332.088 kb on + strand, within BT1858 at 2332.089 kb on - strand, within BT1858 at 2332.089 kb on - strand, within BT1858 at 2332.089 kb on - strand, within BT1858 at 2332.091 kb on - strand, within BT1858 at 2332.091 kb on - strand, within BT1858 at 2332.138 kb on + strand, within BT1858 at 2332.138 kb on + strand, within BT1858 at 2332.181 kb on - strand, within BT1858 at 2332.182 kb on + strand, within BT1858 at 2332.182 kb on + strand, within BT1858 at 2332.182 kb on + strand, within BT1858 at 2332.222 kb on - strand, within BT1858 at 2332.229 kb on + strand, within BT1858 at 2332.229 kb on + strand, within BT1858 at 2332.232 kb on + strand, within BT1858 at 2332.296 kb on - strand, within BT1858 at 2332.332 kb on + strand, within BT1858 at 2332.350 kb on - strand, within BT1858 at 2332.588 kb on - strand at 2332.588 kb on - strand at 2332.646 kb on + strand at 2332.724 kb on - strand, within BT1859 at 2332.833 kb on + strand, within BT1859 at 2332.884 kb on - strand, within BT1859 at 2332.884 kb on - strand, within BT1859 at 2332.983 kb on + strand, within BT1859 at 2332.984 kb on - strand, within BT1859 at 2333.032 kb on + strand, within BT1859 at 2333.056 kb on + strand, within BT1859 at 2333.056 kb on + strand, within BT1859 at 2333.110 kb on + strand, within BT1859 at 2333.122 kb on + strand, within BT1859 at 2333.126 kb on + strand, within BT1859 at 2333.127 kb on - strand, within BT1859 at 2333.156 kb on - strand at 2333.163 kb on - strand at 2333.206 kb on - strand at 2333.214 kb on - strand at 2333.308 kb on + strand at 2333.309 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM remove 2,330,100 - BT1857 0.16 -3.5 2,330,100 - BT1857 0.16 -3.0 2,330,104 - BT1857 0.16 -5.7 2,330,181 + BT1857 0.24 -0.7 2,330,189 + BT1857 0.24 -4.7 2,330,201 + BT1857 0.26 -3.8 2,330,273 + BT1857 0.32 -3.3 2,330,345 + BT1857 0.39 +0.2 2,330,346 - BT1857 0.39 -1.6 2,330,348 - BT1857 0.39 -2.8 2,330,471 - BT1857 0.51 -1.8 2,330,519 - BT1857 0.56 -5.4 2,330,531 - BT1857 0.57 -5.6 2,330,538 - BT1857 0.57 -5.4 2,330,544 - -2.6 2,330,640 - BT1857 0.67 +0.2 2,330,640 - BT1857 0.67 -0.8 2,330,769 + BT1857 0.79 -0.8 2,330,812 + BT1857 0.83 -2.6 2,330,813 - BT1857 0.83 -2.1 2,330,920 - -2.3 2,330,920 - -2.1 2,330,926 - -4.4 2,330,982 - -2.4 2,331,031 + -0.8 2,331,032 - -0.5 2,331,044 - -4.0 2,331,067 - -3.1 2,331,179 - -3.4 2,331,192 + -2.1 2,331,193 - -6.3 2,331,193 - -2.4 2,331,193 - -0.8 2,331,193 - -0.8 2,331,215 + -0.8 2,331,234 - BT1858 0.10 -3.7 2,331,267 - BT1858 0.12 -2.6 2,331,274 - BT1858 0.13 -2.1 2,331,319 + BT1858 0.16 +0.2 2,331,319 + BT1858 0.16 -1.4 2,331,319 + BT1858 0.16 +0.2 2,331,364 - BT1858 0.19 -2.8 2,331,463 - BT1858 0.25 -3.8 2,331,476 + BT1858 0.26 +0.2 2,331,476 + BT1858 0.26 -4.3 2,331,477 - BT1858 0.26 -1.8 2,331,593 + BT1858 0.34 -3.7 2,331,599 + BT1858 0.35 -0.8 2,331,599 + BT1858 0.35 -3.1 2,331,631 + BT1858 0.37 -2.1 2,331,825 - BT1858 0.50 -1.8 2,332,039 + BT1858 0.64 -1.4 2,332,040 - BT1858 0.64 -2.6 2,332,044 + BT1858 0.64 -0.8 2,332,088 + BT1858 0.67 -2.4 2,332,088 + BT1858 0.67 -1.4 2,332,089 - BT1858 0.67 -1.8 2,332,089 - BT1858 0.67 +0.2 2,332,089 - BT1858 0.67 -1.4 2,332,091 - BT1858 0.67 -0.8 2,332,091 - BT1858 0.67 -2.6 2,332,138 + BT1858 0.70 -0.8 2,332,138 + BT1858 0.70 -3.5 2,332,181 - BT1858 0.73 -3.5 2,332,182 + BT1858 0.73 -4.6 2,332,182 + BT1858 0.73 -4.0 2,332,182 + BT1858 0.73 -0.8 2,332,222 - BT1858 0.76 -4.1 2,332,229 + BT1858 0.77 +0.2 2,332,229 + BT1858 0.77 -1.8 2,332,232 + BT1858 0.77 -1.4 2,332,296 - BT1858 0.81 -2.4 2,332,332 + BT1858 0.83 -4.6 2,332,350 - BT1858 0.85 -3.3 2,332,588 - -4.6 2,332,588 - -4.1 2,332,646 + -4.7 2,332,724 - BT1859 0.23 +0.2 2,332,833 + BT1859 0.41 -4.8 2,332,884 - BT1859 0.49 -3.6 2,332,884 - BT1859 0.49 +0.2 2,332,983 + BT1859 0.66 -4.6 2,332,984 - BT1859 0.66 +0.2 2,333,032 + BT1859 0.74 -2.6 2,333,056 + BT1859 0.78 -1.8 2,333,056 + BT1859 0.78 -3.4 2,333,110 + BT1859 0.87 -4.7 2,333,122 + BT1859 0.89 -1.4 2,333,126 + BT1859 0.90 -2.6 2,333,127 - BT1859 0.90 -0.8 2,333,156 - -2.8 2,333,163 - -6.0 2,333,206 - +2.5 2,333,214 - +0.2 2,333,308 + -1.4 2,333,309 - -6.0
Or see this region's nucleotide sequence