Experiment: Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1842 and BT1843 are separated by 117 nucleotides BT1843 and BT1844 overlap by 4 nucleotides BT1844 and BT1845 are separated by 160 nucleotides
BT1842: BT1842 - alpha-L-fucosidase precursor (NCBI ptt file), at 2,313,348 to 2,314,664
BT1842
BT1843: BT1843 - adenylosuccinate synthetase (NCBI ptt file), at 2,314,782 to 2,316,053
BT1843
BT1844: BT1844 - transcriptional regulator, putative iron uptake regulation protein (NCBI ptt file), at 2,316,050 to 2,316,541
BT1844
BT1845: BT1845 - conserved hypothetical protein (NCBI ptt file), at 2,316,702 to 2,317,367
BT1845
Position (kb)
2314
2315
2316
2317 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3
4 at 2313.783 kb on - strand, within BT1842 at 2313.788 kb on + strand, within BT1842 at 2313.788 kb on + strand, within BT1842 at 2313.791 kb on - strand, within BT1842 at 2313.800 kb on + strand, within BT1842 at 2313.800 kb on + strand, within BT1842 at 2313.866 kb on + strand, within BT1842 at 2313.895 kb on - strand, within BT1842 at 2313.936 kb on - strand, within BT1842 at 2313.943 kb on - strand, within BT1842 at 2313.960 kb on + strand, within BT1842 at 2313.979 kb on + strand, within BT1842 at 2313.979 kb on + strand, within BT1842 at 2314.067 kb on + strand, within BT1842 at 2314.108 kb on + strand, within BT1842 at 2314.201 kb on + strand, within BT1842 at 2314.204 kb on + strand, within BT1842 at 2314.304 kb on - strand, within BT1842 at 2314.357 kb on + strand at 2314.361 kb on + strand, within BT1842 at 2314.361 kb on + strand, within BT1842 at 2314.395 kb on - strand, within BT1842 at 2314.441 kb on + strand, within BT1842 at 2314.463 kb on + strand, within BT1842 at 2314.501 kb on + strand, within BT1842 at 2314.532 kb on + strand, within BT1842 at 2314.629 kb on + strand at 2314.629 kb on + strand at 2314.632 kb on + strand at 2314.633 kb on - strand at 2314.636 kb on - strand at 2314.636 kb on - strand at 2314.636 kb on - strand at 2314.675 kb on - strand at 2314.678 kb on + strand at 2314.740 kb on - strand at 2314.745 kb on - strand at 2314.745 kb on - strand at 2314.746 kb on + strand at 2314.762 kb on + strand at 2314.793 kb on + strand at 2314.796 kb on - strand at 2314.796 kb on - strand at 2314.879 kb on + strand at 2314.879 kb on + strand at 2314.929 kb on - strand, within BT1843 at 2314.957 kb on + strand, within BT1843 at 2314.957 kb on + strand, within BT1843 at 2314.959 kb on + strand, within BT1843 at 2314.959 kb on + strand, within BT1843 at 2314.960 kb on - strand, within BT1843 at 2315.017 kb on - strand, within BT1843 at 2315.023 kb on + strand, within BT1843 at 2315.023 kb on + strand, within BT1843 at 2315.045 kb on + strand, within BT1843 at 2315.101 kb on - strand, within BT1843 at 2315.101 kb on - strand, within BT1843 at 2315.189 kb on + strand, within BT1843 at 2315.245 kb on - strand, within BT1843 at 2315.302 kb on - strand, within BT1843 at 2315.349 kb on - strand, within BT1843 at 2315.486 kb on + strand, within BT1843 at 2315.524 kb on + strand, within BT1843 at 2315.649 kb on - strand, within BT1843 at 2315.705 kb on - strand, within BT1843 at 2315.901 kb on + strand, within BT1843 at 2315.901 kb on + strand, within BT1843 at 2315.901 kb on + strand, within BT1843 at 2315.905 kb on + strand, within BT1843 at 2315.930 kb on + strand at 2315.930 kb on + strand at 2315.930 kb on + strand at 2315.931 kb on - strand at 2315.931 kb on - strand at 2315.931 kb on - strand at 2315.974 kb on + strand at 2315.985 kb on + strand at 2315.989 kb on - strand at 2316.034 kb on - strand at 2316.046 kb on - strand at 2316.092 kb on - strand at 2316.127 kb on + strand, within BT1844 at 2316.128 kb on - strand, within BT1844 at 2316.130 kb on - strand, within BT1844 at 2316.229 kb on + strand, within BT1844 at 2316.233 kb on + strand, within BT1844 at 2316.233 kb on + strand at 2316.233 kb on + strand, within BT1844 at 2316.274 kb on - strand, within BT1844 at 2316.337 kb on + strand, within BT1844 at 2316.341 kb on - strand, within BT1844 at 2316.341 kb on - strand, within BT1844 at 2316.394 kb on - strand, within BT1844 at 2316.400 kb on + strand, within BT1844 at 2316.429 kb on + strand, within BT1844 at 2316.429 kb on + strand, within BT1844 at 2316.490 kb on - strand, within BT1844 at 2316.514 kb on + strand at 2316.568 kb on + strand at 2316.607 kb on + strand at 2316.612 kb on - strand at 2316.618 kb on + strand at 2316.619 kb on - strand at 2316.625 kb on + strand at 2316.628 kb on + strand at 2316.632 kb on + strand at 2316.633 kb on - strand at 2316.642 kb on - strand at 2316.642 kb on - strand at 2316.645 kb on + strand at 2316.682 kb on + strand at 2316.683 kb on - strand at 2316.683 kb on - strand at 2316.683 kb on - strand at 2316.687 kb on - strand at 2316.693 kb on + strand at 2316.697 kb on - strand at 2316.697 kb on - strand at 2316.700 kb on + strand at 2316.825 kb on - strand, within BT1845 at 2316.825 kb on - strand, within BT1845 at 2316.825 kb on - strand, within BT1845 at 2316.826 kb on + strand, within BT1845 at 2316.827 kb on - strand, within BT1845
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM remove 2,313,783 - BT1842 0.33 +0.0 2,313,788 + BT1842 0.33 +0.1 2,313,788 + BT1842 0.33 -0.1 2,313,791 - BT1842 0.34 -3.0 2,313,800 + BT1842 0.34 -0.1 2,313,800 + BT1842 0.34 +0.2 2,313,866 + BT1842 0.39 -1.8 2,313,895 - BT1842 0.42 -1.4 2,313,936 - BT1842 0.45 -0.5 2,313,943 - BT1842 0.45 -0.1 2,313,960 + BT1842 0.46 -2.1 2,313,979 + BT1842 0.48 -0.8 2,313,979 + BT1842 0.48 -0.1 2,314,067 + BT1842 0.55 -1.4 2,314,108 + BT1842 0.58 +0.0 2,314,201 + BT1842 0.65 +4.4 2,314,204 + BT1842 0.65 +2.2 2,314,304 - BT1842 0.73 -1.8 2,314,357 + +0.2 2,314,361 + BT1842 0.77 -0.8 2,314,361 + BT1842 0.77 -0.8 2,314,395 - BT1842 0.79 -0.5 2,314,441 + BT1842 0.83 +0.7 2,314,463 + BT1842 0.85 +0.2 2,314,501 + BT1842 0.88 -1.4 2,314,532 + BT1842 0.90 -2.1 2,314,629 + -0.8 2,314,629 + -3.0 2,314,632 + -0.3 2,314,633 - -2.1 2,314,636 - -0.1 2,314,636 - -2.6 2,314,636 - -0.8 2,314,675 - -1.4 2,314,678 + -0.8 2,314,740 - -0.8 2,314,745 - -0.8 2,314,745 - +1.2 2,314,746 + -0.8 2,314,762 + -0.8 2,314,793 + -2.1 2,314,796 - -1.4 2,314,796 - +0.6 2,314,879 + -3.4 2,314,879 + -2.8 2,314,929 - BT1843 0.12 -1.8 2,314,957 + BT1843 0.14 -0.8 2,314,957 + BT1843 0.14 -1.8 2,314,959 + BT1843 0.14 -3.0 2,314,959 + BT1843 0.14 -2.6 2,314,960 - BT1843 0.14 -3.1 2,315,017 - BT1843 0.18 -1.4 2,315,023 + BT1843 0.19 -1.8 2,315,023 + BT1843 0.19 -0.8 2,315,045 + BT1843 0.21 -1.4 2,315,101 - BT1843 0.25 -1.4 2,315,101 - BT1843 0.25 +0.2 2,315,189 + BT1843 0.32 -0.8 2,315,245 - BT1843 0.36 -4.1 2,315,302 - BT1843 0.41 -2.4 2,315,349 - BT1843 0.45 -2.1 2,315,486 + BT1843 0.55 -3.6 2,315,524 + BT1843 0.58 -2.7 2,315,649 - BT1843 0.68 -2.1 2,315,705 - BT1843 0.73 -5.9 2,315,901 + BT1843 0.88 -3.0 2,315,901 + BT1843 0.88 -1.4 2,315,901 + BT1843 0.88 -0.8 2,315,905 + BT1843 0.88 -5.3 2,315,930 + -2.6 2,315,930 + -0.8 2,315,930 + -1.4 2,315,931 - -3.1 2,315,931 - -2.9 2,315,931 - -1.4 2,315,974 + -0.8 2,315,985 + -3.8 2,315,989 - -4.1 2,316,034 - -1.4 2,316,046 - -4.0 2,316,092 - -3.0 2,316,127 + BT1844 0.16 -2.1 2,316,128 - BT1844 0.16 +0.2 2,316,130 - BT1844 0.16 -0.6 2,316,229 + BT1844 0.36 -3.0 2,316,233 + BT1844 0.37 -2.6 2,316,233 + -1.4 2,316,233 + BT1844 0.37 -1.8 2,316,274 - BT1844 0.46 -0.8 2,316,337 + BT1844 0.58 -2.6 2,316,341 - BT1844 0.59 -2.1 2,316,341 - BT1844 0.59 -1.8 2,316,394 - BT1844 0.70 -2.4 2,316,400 + BT1844 0.71 -0.8 2,316,429 + BT1844 0.77 -2.6 2,316,429 + BT1844 0.77 -2.8 2,316,490 - BT1844 0.89 +0.2 2,316,514 + -1.8 2,316,568 + -2.1 2,316,607 + -3.0 2,316,612 - -2.1 2,316,618 + -1.9 2,316,619 - -0.8 2,316,625 + -1.8 2,316,628 + +0.2 2,316,632 + -1.2 2,316,633 - -0.8 2,316,642 - +0.2 2,316,642 - +1.5 2,316,645 + +0.2 2,316,682 + -0.7 2,316,683 - -1.8 2,316,683 - -1.4 2,316,683 - -2.4 2,316,687 - -0.4 2,316,693 + -0.4 2,316,697 - -0.8 2,316,697 - -1.8 2,316,700 + -0.8 2,316,825 - BT1845 0.18 -0.8 2,316,825 - BT1845 0.18 +1.2 2,316,825 - BT1845 0.18 -1.4 2,316,826 + BT1845 0.19 +0.2 2,316,827 - BT1845 0.19 +0.2
Or see this region's nucleotide sequence