Experiment: Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1607 and BT1608 are separated by 154 nucleotides BT1608 and tRNA-His are separated by 64 nucleotides tRNA-His and BT1609 are separated by 74 nucleotides BT1609 and BT1610 are separated by 70 nucleotides
BT1607: BT1607 - methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase (NCBI ptt file), at 1,978,425 to 1,979,306
BT1607
BT1608: BT1608 - capsule biosynthesis protein capA (NCBI ptt file), at 1,979,461 to 1,980,597
BT1608
BT_t29: tRNA-His - tRNA-His (RefSeq), at 1,980,662 to 1,980,740
tRNA-His
BT1609: BT1609 - conserved hypothetical protein (NCBI ptt file), at 1,980,815 to 1,981,051
BT1609
BT1610: BT1610 - DNA polymerase III subunit gamma/tau (NCBI ptt file), at 1,981,122 to 1,982,981
BT1610
Position (kb)
1979
1980
1981 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 1978.570 kb on + strand, within BT1607 at 1978.630 kb on - strand, within BT1607 at 1978.694 kb on - strand, within BT1607 at 1978.704 kb on - strand, within BT1607 at 1978.704 kb on - strand, within BT1607 at 1978.708 kb on + strand, within BT1607 at 1978.731 kb on - strand at 1978.830 kb on + strand, within BT1607 at 1978.834 kb on + strand, within BT1607 at 1979.045 kb on + strand, within BT1607 at 1979.046 kb on - strand, within BT1607 at 1979.254 kb on + strand at 1979.294 kb on + strand at 1979.397 kb on + strand at 1979.398 kb on + strand at 1979.398 kb on + strand at 1979.443 kb on + strand at 1979.443 kb on + strand at 1979.444 kb on - strand at 1979.444 kb on - strand at 1979.461 kb on - strand at 1979.478 kb on - strand at 1979.508 kb on + strand at 1979.532 kb on + strand at 1979.536 kb on - strand at 1979.536 kb on - strand at 1979.546 kb on + strand at 1979.547 kb on - strand at 1979.547 kb on - strand at 1979.714 kb on + strand, within BT1608 at 1979.749 kb on + strand, within BT1608 at 1979.785 kb on + strand, within BT1608 at 1979.842 kb on - strand, within BT1608 at 1979.879 kb on + strand, within BT1608 at 1979.880 kb on - strand, within BT1608 at 1979.888 kb on + strand, within BT1608 at 1979.991 kb on - strand, within BT1608 at 1980.004 kb on + strand, within BT1608 at 1980.057 kb on + strand, within BT1608 at 1980.181 kb on + strand, within BT1608 at 1980.203 kb on + strand, within BT1608 at 1980.204 kb on - strand, within BT1608 at 1980.212 kb on + strand, within BT1608 at 1980.288 kb on - strand, within BT1608 at 1980.288 kb on - strand, within BT1608 at 1980.405 kb on + strand, within BT1608 at 1980.405 kb on + strand, within BT1608 at 1980.413 kb on + strand, within BT1608 at 1980.414 kb on - strand, within BT1608 at 1980.458 kb on + strand, within BT1608 at 1980.504 kb on + strand at 1980.912 kb on + strand, within BT1609 at 1980.921 kb on + strand, within BT1609 at 1980.921 kb on + strand, within BT1609 at 1980.922 kb on - strand, within BT1609 at 1980.977 kb on - strand, within BT1609 at 1980.991 kb on + strand, within BT1609 at 1981.044 kb on + strand at 1981.080 kb on + strand at 1981.081 kb on - strand at 1981.086 kb on - strand at 1981.108 kb on + strand at 1981.118 kb on + strand at 1981.128 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM remove 1,978,570 + BT1607 0.16 -2.4 1,978,630 - BT1607 0.23 -0.8 1,978,694 - BT1607 0.30 -1.4 1,978,704 - BT1607 0.32 -1.5 1,978,704 - BT1607 0.32 -5.4 1,978,708 + BT1607 0.32 -3.5 1,978,731 - -1.4 1,978,830 + BT1607 0.46 -3.9 1,978,834 + BT1607 0.46 -2.6 1,979,045 + BT1607 0.70 -0.8 1,979,046 - BT1607 0.70 -0.8 1,979,254 + -0.8 1,979,294 + -3.0 1,979,397 + -1.0 1,979,398 + -0.2 1,979,398 + -2.0 1,979,443 + +1.2 1,979,443 + -2.3 1,979,444 - -1.3 1,979,444 - -3.3 1,979,461 - -3.0 1,979,478 - +0.2 1,979,508 + -0.8 1,979,532 + -2.7 1,979,536 - -1.7 1,979,536 - -0.8 1,979,546 + +0.2 1,979,547 - -3.9 1,979,547 - -4.3 1,979,714 + BT1608 0.22 -1.4 1,979,749 + BT1608 0.25 -2.1 1,979,785 + BT1608 0.28 -1.8 1,979,842 - BT1608 0.34 -1.4 1,979,879 + BT1608 0.37 -1.8 1,979,880 - BT1608 0.37 -0.8 1,979,888 + BT1608 0.38 -3.4 1,979,991 - BT1608 0.47 +0.2 1,980,004 + BT1608 0.48 -1.8 1,980,057 + BT1608 0.52 +0.2 1,980,181 + BT1608 0.63 -0.9 1,980,203 + BT1608 0.65 -0.4 1,980,204 - BT1608 0.65 -2.4 1,980,212 + BT1608 0.66 -2.4 1,980,288 - BT1608 0.73 -5.3 1,980,288 - BT1608 0.73 -2.4 1,980,405 + BT1608 0.83 -0.8 1,980,405 + BT1608 0.83 -0.8 1,980,413 + BT1608 0.84 +0.2 1,980,414 - BT1608 0.84 -0.8 1,980,458 + BT1608 0.88 -0.8 1,980,504 + -2.4 1,980,912 + BT1609 0.41 -2.1 1,980,921 + BT1609 0.45 -0.2 1,980,921 + BT1609 0.45 -0.8 1,980,922 - BT1609 0.45 +0.2 1,980,977 - BT1609 0.68 +4.0 1,980,991 + BT1609 0.74 -1.0 1,981,044 + +0.2 1,981,080 + -1.4 1,981,081 - -1.3 1,981,086 - -1.2 1,981,108 + +0.2 1,981,118 + -0.2 1,981,128 + -3.4
Or see this region's nucleotide sequence