Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT1608

Experiment: Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT1607 and BT1608 are separated by 154 nucleotidesBT1608 and tRNA-His are separated by 64 nucleotidestRNA-His and BT1609 are separated by 74 nucleotidesBT1609 and BT1610 are separated by 70 nucleotides BT1607: BT1607 - methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase (NCBI ptt file), at 1,978,425 to 1,979,306 BT1607 BT1608: BT1608 - capsule biosynthesis protein capA (NCBI ptt file), at 1,979,461 to 1,980,597 BT1608 BT_t29: tRNA-His - tRNA-His (RefSeq), at 1,980,662 to 1,980,740 tRNA-His BT1609: BT1609 - conserved hypothetical protein (NCBI ptt file), at 1,980,815 to 1,981,051 BT1609 BT1610: BT1610 - DNA polymerase III subunit gamma/tau (NCBI ptt file), at 1,981,122 to 1,982,981 BT1610 Position (kb) 1979 1980 1981Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3 4at 1978.570 kb on + strand, within BT1607at 1978.630 kb on - strand, within BT1607at 1978.694 kb on - strand, within BT1607at 1978.704 kb on - strand, within BT1607at 1978.704 kb on - strand, within BT1607at 1978.708 kb on + strand, within BT1607at 1978.731 kb on - strandat 1978.830 kb on + strand, within BT1607at 1978.834 kb on + strand, within BT1607at 1979.045 kb on + strand, within BT1607at 1979.046 kb on - strand, within BT1607at 1979.254 kb on + strandat 1979.294 kb on + strandat 1979.397 kb on + strandat 1979.398 kb on + strandat 1979.398 kb on + strandat 1979.443 kb on + strandat 1979.443 kb on + strandat 1979.444 kb on - strandat 1979.444 kb on - strandat 1979.461 kb on - strandat 1979.478 kb on - strandat 1979.508 kb on + strandat 1979.532 kb on + strandat 1979.536 kb on - strandat 1979.536 kb on - strandat 1979.546 kb on + strandat 1979.547 kb on - strandat 1979.547 kb on - strandat 1979.714 kb on + strand, within BT1608at 1979.749 kb on + strand, within BT1608at 1979.785 kb on + strand, within BT1608at 1979.842 kb on - strand, within BT1608at 1979.879 kb on + strand, within BT1608at 1979.880 kb on - strand, within BT1608at 1979.888 kb on + strand, within BT1608at 1979.991 kb on - strand, within BT1608at 1980.004 kb on + strand, within BT1608at 1980.057 kb on + strand, within BT1608at 1980.181 kb on + strand, within BT1608at 1980.203 kb on + strand, within BT1608at 1980.204 kb on - strand, within BT1608at 1980.212 kb on + strand, within BT1608at 1980.288 kb on - strand, within BT1608at 1980.288 kb on - strand, within BT1608at 1980.405 kb on + strand, within BT1608at 1980.405 kb on + strand, within BT1608at 1980.413 kb on + strand, within BT1608at 1980.414 kb on - strand, within BT1608at 1980.458 kb on + strand, within BT1608at 1980.504 kb on + strandat 1980.912 kb on + strand, within BT1609at 1980.921 kb on + strand, within BT1609at 1980.921 kb on + strand, within BT1609at 1980.922 kb on - strand, within BT1609at 1980.977 kb on - strand, within BT1609at 1980.991 kb on + strand, within BT1609at 1981.044 kb on + strandat 1981.080 kb on + strandat 1981.081 kb on - strandat 1981.086 kb on - strandat 1981.108 kb on + strandat 1981.118 kb on + strandat 1981.128 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM
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1,978,570 + BT1607 0.16 -2.4
1,978,630 - BT1607 0.23 -0.8
1,978,694 - BT1607 0.30 -1.4
1,978,704 - BT1607 0.32 -1.5
1,978,704 - BT1607 0.32 -5.4
1,978,708 + BT1607 0.32 -3.5
1,978,731 - -1.4
1,978,830 + BT1607 0.46 -3.9
1,978,834 + BT1607 0.46 -2.6
1,979,045 + BT1607 0.70 -0.8
1,979,046 - BT1607 0.70 -0.8
1,979,254 + -0.8
1,979,294 + -3.0
1,979,397 + -1.0
1,979,398 + -0.2
1,979,398 + -2.0
1,979,443 + +1.2
1,979,443 + -2.3
1,979,444 - -1.3
1,979,444 - -3.3
1,979,461 - -3.0
1,979,478 - +0.2
1,979,508 + -0.8
1,979,532 + -2.7
1,979,536 - -1.7
1,979,536 - -0.8
1,979,546 + +0.2
1,979,547 - -3.9
1,979,547 - -4.3
1,979,714 + BT1608 0.22 -1.4
1,979,749 + BT1608 0.25 -2.1
1,979,785 + BT1608 0.28 -1.8
1,979,842 - BT1608 0.34 -1.4
1,979,879 + BT1608 0.37 -1.8
1,979,880 - BT1608 0.37 -0.8
1,979,888 + BT1608 0.38 -3.4
1,979,991 - BT1608 0.47 +0.2
1,980,004 + BT1608 0.48 -1.8
1,980,057 + BT1608 0.52 +0.2
1,980,181 + BT1608 0.63 -0.9
1,980,203 + BT1608 0.65 -0.4
1,980,204 - BT1608 0.65 -2.4
1,980,212 + BT1608 0.66 -2.4
1,980,288 - BT1608 0.73 -5.3
1,980,288 - BT1608 0.73 -2.4
1,980,405 + BT1608 0.83 -0.8
1,980,405 + BT1608 0.83 -0.8
1,980,413 + BT1608 0.84 +0.2
1,980,414 - BT1608 0.84 -0.8
1,980,458 + BT1608 0.88 -0.8
1,980,504 + -2.4
1,980,912 + BT1609 0.41 -2.1
1,980,921 + BT1609 0.45 -0.2
1,980,921 + BT1609 0.45 -0.8
1,980,922 - BT1609 0.45 +0.2
1,980,977 - BT1609 0.68 +4.0
1,980,991 + BT1609 0.74 -1.0
1,981,044 + +0.2
1,981,080 + -1.4
1,981,081 - -1.3
1,981,086 - -1.2
1,981,108 + +0.2
1,981,118 + -0.2
1,981,128 + -3.4

Or see this region's nucleotide sequence