Experiment: Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1374 and BT1375 are separated by 78 nucleotides BT1375 and BT1376 are separated by 13 nucleotides BT1376 and BT1377 overlap by 16 nucleotides
BT1374: BT1374 - diaminopimelate decarboxylase (EC 4.1.1.20) (from data) , at 1,702,211 to 1,703,371
BT1374
BT1375: BT1375 - aspartokinase (NCBI ptt file), at 1,703,450 to 1,704,769
BT1375
BT1376: BT1376 - putative ATP-binding protein involved in cell division (NCBI ptt file), at 1,704,783 to 1,705,508
BT1376
BT1377: BT1377 - phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphohydrolase (NCBI ptt file), at 1,705,493 to 1,706,104
BT1377
Position (kb)
1703
1704
1705 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1702.518 kb on - strand, within BT1374 at 1702.621 kb on - strand, within BT1374 at 1702.705 kb on + strand, within BT1374 at 1702.705 kb on + strand, within BT1374 at 1702.706 kb on - strand, within BT1374 at 1702.713 kb on - strand, within BT1374 at 1702.720 kb on + strand, within BT1374 at 1702.721 kb on - strand, within BT1374 at 1702.882 kb on + strand, within BT1374 at 1702.883 kb on - strand, within BT1374 at 1703.201 kb on - strand, within BT1374 at 1703.241 kb on - strand, within BT1374 at 1703.247 kb on + strand, within BT1374 at 1703.252 kb on - strand, within BT1374 at 1703.291 kb on - strand at 1703.291 kb on - strand at 1703.317 kb on - strand at 1703.320 kb on - strand at 1703.360 kb on + strand at 1703.361 kb on - strand at 1703.389 kb on + strand at 1703.439 kb on + strand at 1703.452 kb on - strand at 1703.528 kb on - strand at 1703.533 kb on - strand at 1703.536 kb on + strand at 1703.630 kb on + strand, within BT1375 at 1703.634 kb on - strand, within BT1375 at 1703.645 kb on - strand, within BT1375 at 1703.651 kb on - strand, within BT1375 at 1703.723 kb on + strand, within BT1375 at 1703.779 kb on - strand, within BT1375 at 1703.830 kb on + strand, within BT1375 at 1703.857 kb on + strand, within BT1375 at 1703.858 kb on - strand, within BT1375 at 1703.858 kb on - strand, within BT1375 at 1703.958 kb on - strand, within BT1375 at 1703.963 kb on + strand, within BT1375 at 1703.964 kb on - strand, within BT1375 at 1703.966 kb on - strand, within BT1375 at 1703.977 kb on + strand, within BT1375 at 1703.978 kb on - strand, within BT1375 at 1704.022 kb on - strand, within BT1375 at 1704.022 kb on - strand, within BT1375 at 1704.022 kb on - strand, within BT1375 at 1704.123 kb on - strand, within BT1375 at 1704.172 kb on - strand, within BT1375 at 1704.296 kb on - strand, within BT1375 at 1704.298 kb on - strand, within BT1375 at 1704.298 kb on - strand, within BT1375 at 1704.299 kb on + strand, within BT1375 at 1704.300 kb on - strand, within BT1375 at 1704.329 kb on - strand, within BT1375 at 1704.521 kb on - strand, within BT1375 at 1704.613 kb on + strand, within BT1375 at 1704.785 kb on - strand at 1704.788 kb on + strand at 1704.795 kb on - strand at 1704.832 kb on + strand at 1704.833 kb on - strand at 1704.875 kb on - strand, within BT1376 at 1704.877 kb on - strand, within BT1376 at 1704.877 kb on - strand, within BT1376 at 1705.211 kb on - strand, within BT1376 at 1705.305 kb on - strand, within BT1376 at 1705.307 kb on + strand, within BT1376 at 1705.406 kb on - strand, within BT1376 at 1705.406 kb on - strand, within BT1376 at 1705.574 kb on + strand, within BT1377 at 1705.640 kb on - strand, within BT1377 at 1705.723 kb on - strand, within BT1377 at 1705.723 kb on - strand, within BT1377
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM remove 1,702,518 - BT1374 0.26 -4.7 1,702,621 - BT1374 0.35 -1.3 1,702,705 + BT1374 0.43 -1.7 1,702,705 + BT1374 0.43 -2.6 1,702,706 - BT1374 0.43 -2.3 1,702,713 - BT1374 0.43 -0.7 1,702,720 + BT1374 0.44 -2.3 1,702,721 - BT1374 0.44 -4.0 1,702,882 + BT1374 0.58 -1.3 1,702,883 - BT1374 0.58 -1.3 1,703,201 - BT1374 0.85 -2.1 1,703,241 - BT1374 0.89 +0.3 1,703,247 + BT1374 0.89 -2.7 1,703,252 - BT1374 0.90 -4.3 1,703,291 - -0.7 1,703,291 - +0.3 1,703,317 - -1.3 1,703,320 - +0.3 1,703,360 + -3.1 1,703,361 - -0.7 1,703,389 + -2.3 1,703,439 + -3.9 1,703,452 - -1.7 1,703,528 - -2.6 1,703,533 - -2.3 1,703,536 + -2.9 1,703,630 + BT1375 0.14 +0.3 1,703,634 - BT1375 0.14 -5.0 1,703,645 - BT1375 0.15 +0.3 1,703,651 - BT1375 0.15 -2.1 1,703,723 + BT1375 0.21 +0.3 1,703,779 - BT1375 0.25 -1.3 1,703,830 + BT1375 0.29 +0.3 1,703,857 + BT1375 0.31 +0.3 1,703,858 - BT1375 0.31 -3.6 1,703,858 - BT1375 0.31 -0.7 1,703,958 - BT1375 0.38 -2.6 1,703,963 + BT1375 0.39 -1.3 1,703,964 - BT1375 0.39 -1.7 1,703,966 - BT1375 0.39 -0.7 1,703,977 + BT1375 0.40 -1.7 1,703,978 - BT1375 0.40 -2.7 1,704,022 - BT1375 0.43 -3.8 1,704,022 - BT1375 0.43 -2.6 1,704,022 - BT1375 0.43 -4.1 1,704,123 - BT1375 0.51 -2.3 1,704,172 - BT1375 0.55 -3.3 1,704,296 - BT1375 0.64 -4.4 1,704,298 - BT1375 0.64 -3.2 1,704,298 - BT1375 0.64 -5.2 1,704,299 + BT1375 0.64 -3.2 1,704,300 - BT1375 0.64 -5.1 1,704,329 - BT1375 0.67 -2.7 1,704,521 - BT1375 0.81 -3.1 1,704,613 + BT1375 0.88 -2.3 1,704,785 - -2.3 1,704,788 + -0.8 1,704,795 - -1.1 1,704,832 + -0.8 1,704,833 - +1.2 1,704,875 - BT1376 0.13 -1.8 1,704,877 - BT1376 0.13 -1.3 1,704,877 - BT1376 0.13 -0.5 1,705,211 - BT1376 0.59 -2.1 1,705,305 - BT1376 0.72 -0.8 1,705,307 + BT1376 0.72 -2.6 1,705,406 - BT1376 0.86 +1.2 1,705,406 - BT1376 0.86 -2.3 1,705,574 + BT1377 0.13 -2.6 1,705,640 - BT1377 0.24 -3.5 1,705,723 - BT1377 0.38 -1.3 1,705,723 - BT1377 0.38 -3.1
Or see this region's nucleotide sequence