Experiment: Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1229 and BT1230 are separated by 117 nucleotides BT1230 and BT1231 are separated by 520 nucleotides BT1231 and tRNA-Ser are separated by 95 nucleotides tRNA-Ser and tRNA-Glu are separated by 63 nucleotides
BT1229: BT1229 - conserved hypothetical protein, putative membrane protein (NCBI ptt file), at 1,531,038 to 1,532,705
BT1229
BT1230: BT1230 - hypothetical protein (NCBI ptt file), at 1,532,823 to 1,533,323
BT1230
BT1231: BT1231 - hypothetical protein (NCBI ptt file), at 1,533,844 to 1,534,047
BT1231
BT_t21: tRNA-Ser - tRNA-Ser (RefSeq), at 1,534,143 to 1,534,232
tRNA-Ser
BT_t22: tRNA-Glu - tRNA-Glu (RefSeq), at 1,534,296 to 1,534,367
tRNA-Glu
Position (kb)
1532
1533
1534 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1531.854 kb on - strand, within BT1229 at 1532.476 kb on + strand, within BT1229 at 1532.822 kb on + strand at 1532.822 kb on + strand at 1532.823 kb on - strand at 1532.834 kb on + strand at 1532.851 kb on - strand at 1532.865 kb on + strand at 1533.034 kb on + strand, within BT1230 at 1533.093 kb on + strand, within BT1230 at 1533.093 kb on + strand, within BT1230 at 1533.093 kb on + strand, within BT1230 at 1533.311 kb on + strand at 1533.318 kb on - strand at 1533.438 kb on + strand at 1533.438 kb on + strand at 1533.438 kb on + strand at 1533.478 kb on + strand at 1533.493 kb on + strand at 1533.545 kb on + strand at 1533.563 kb on + strand at 1533.597 kb on + strand at 1533.862 kb on - strand at 1533.912 kb on + strand, within BT1231 at 1534.036 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM remove 1,531,854 - BT1229 0.49 +1.0 1,532,476 + BT1229 0.86 -1.3 1,532,822 + -0.6 1,532,822 + -0.7 1,532,823 - +0.3 1,532,834 + +0.3 1,532,851 - -2.9 1,532,865 + +0.3 1,533,034 + BT1230 0.42 -2.3 1,533,093 + BT1230 0.54 -1.1 1,533,093 + BT1230 0.54 -3.1 1,533,093 + BT1230 0.54 -0.7 1,533,311 + +0.3 1,533,318 - -0.7 1,533,438 + -1.7 1,533,438 + -1.2 1,533,438 + +0.3 1,533,478 + -1.2 1,533,493 + -1.2 1,533,545 + +1.8 1,533,563 + -3.1 1,533,597 + -0.7 1,533,862 - -0.7 1,533,912 + BT1231 0.33 -2.1 1,534,036 + -2.7
Or see this region's nucleotide sequence