Experiment: Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1172 and BT1173 are separated by 265 nucleotides BT1173 and BT1174 are separated by 13 nucleotides BT1174 and BT1175 are separated by 135 nucleotides
BT1172: BT1172 - DNA primase/helicase (NCBI ptt file), at 1,459,791 to 1,461,590
BT1172
BT1173: BT1173 - hypothetical protein (NCBI ptt file), at 1,461,856 to 1,462,842
BT1173
BT1174: BT1174 - hypothetical protein (NCBI ptt file), at 1,462,856 to 1,463,299
BT1174
BT1175: BT1175 - hypothetical protein (NCBI ptt file), at 1,463,435 to 1,464,553
BT1175
Position (kb)
1461
1462
1463 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1461.288 kb on - strand, within BT1172 at 1461.603 kb on + strand at 1461.656 kb on - strand at 1461.681 kb on - strand at 1461.681 kb on - strand at 1461.857 kb on + strand at 1461.857 kb on + strand at 1461.945 kb on - strand at 1461.980 kb on + strand, within BT1173 at 1462.218 kb on - strand, within BT1173 at 1462.518 kb on + strand, within BT1173 at 1462.519 kb on - strand, within BT1173 at 1462.725 kb on + strand, within BT1173 at 1462.739 kb on + strand, within BT1173 at 1462.798 kb on - strand at 1462.847 kb on + strand at 1463.085 kb on - strand, within BT1174 at 1463.085 kb on - strand, within BT1174 at 1463.097 kb on + strand, within BT1174 at 1463.098 kb on - strand, within BT1174 at 1463.098 kb on - strand, within BT1174 at 1463.101 kb on + strand, within BT1174 at 1463.130 kb on + strand, within BT1174 at 1463.145 kb on + strand, within BT1174 at 1463.145 kb on + strand, within BT1174 at 1463.190 kb on + strand, within BT1174 at 1463.191 kb on - strand, within BT1174 at 1463.209 kb on - strand, within BT1174 at 1463.210 kb on + strand, within BT1174 at 1463.211 kb on - strand, within BT1174 at 1463.214 kb on - strand, within BT1174 at 1463.278 kb on + strand at 1463.279 kb on - strand at 1463.297 kb on + strand at 1463.381 kb on - strand at 1463.387 kb on + strand at 1463.388 kb on - strand at 1463.398 kb on + strand at 1463.451 kb on + strand at 1463.452 kb on - strand at 1463.537 kb on - strand at 1463.560 kb on - strand, within BT1175 at 1463.560 kb on - strand, within BT1175 at 1463.687 kb on - strand, within BT1175
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM remove 1,461,288 - BT1172 0.83 +1.8 1,461,603 + -1.4 1,461,656 - +0.2 1,461,681 - -3.2 1,461,681 - +1.5 1,461,857 + +2.2 1,461,857 + -1.0 1,461,945 - -1.4 1,461,980 + BT1173 0.13 -1.4 1,462,218 - BT1173 0.37 -2.6 1,462,518 + BT1173 0.67 -2.2 1,462,519 - BT1173 0.67 -1.7 1,462,725 + BT1173 0.88 +0.2 1,462,739 + BT1173 0.89 -1.4 1,462,798 - -1.8 1,462,847 + -1.8 1,463,085 - BT1174 0.52 -1.4 1,463,085 - BT1174 0.52 -0.8 1,463,097 + BT1174 0.54 -1.4 1,463,098 - BT1174 0.55 +0.9 1,463,098 - BT1174 0.55 -0.8 1,463,101 + BT1174 0.55 -1.8 1,463,130 + BT1174 0.62 -1.3 1,463,145 + BT1174 0.65 -1.4 1,463,145 + BT1174 0.65 -2.6 1,463,190 + BT1174 0.75 +0.2 1,463,191 - BT1174 0.75 +0.2 1,463,209 - BT1174 0.80 +0.2 1,463,210 + BT1174 0.80 -2.0 1,463,211 - BT1174 0.80 -2.1 1,463,214 - BT1174 0.81 +0.2 1,463,278 + +0.4 1,463,279 - -1.4 1,463,297 + -0.8 1,463,381 - -3.4 1,463,387 + -0.9 1,463,388 - -2.1 1,463,398 + -0.8 1,463,451 + -0.8 1,463,452 - -0.8 1,463,537 - -0.8 1,463,560 - BT1175 0.11 -0.8 1,463,560 - BT1175 0.11 -0.8 1,463,687 - BT1175 0.23 -2.1
Or see this region's nucleotide sequence