Experiment: Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tRNA-Gly and BT0954 are separated by 741 nucleotides BT0954 and BT0955 are separated by 6 nucleotides
BT_t18: tRNA-Gly - tRNA-Gly (RefSeq), at 1,163,463 to 1,163,535
tRNA-Gly
BT0954: BT0954 - conserved hypothetical protein (NCBI ptt file), at 1,164,277 to 1,165,689
BT0954
BT0955: BT0955 - conserved hypothetical protein, putative membrane protein (NCBI ptt file), at 1,165,696 to 1,166,685
BT0955
Position (kb)
1164
1165
1166 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1163.286 kb on + strand at 1163.286 kb on + strand at 1163.287 kb on - strand at 1163.315 kb on + strand at 1163.322 kb on + strand at 1163.334 kb on - strand at 1163.334 kb on - strand at 1163.334 kb on - strand at 1163.443 kb on - strand at 1163.507 kb on + strand, within tRNA-Gly at 1163.539 kb on - strand at 1163.545 kb on - strand at 1163.640 kb on - strand at 1163.645 kb on - strand at 1163.646 kb on + strand at 1163.843 kb on + strand at 1163.864 kb on - strand at 1163.908 kb on + strand at 1163.921 kb on + strand at 1163.943 kb on - strand at 1164.068 kb on + strand at 1164.070 kb on + strand at 1164.071 kb on - strand at 1164.103 kb on - strand at 1164.144 kb on - strand at 1164.172 kb on + strand at 1164.264 kb on + strand at 1164.270 kb on - strand at 1164.543 kb on + strand, within BT0954 at 1164.566 kb on + strand, within BT0954 at 1164.593 kb on + strand, within BT0954 at 1164.618 kb on - strand, within BT0954 at 1164.809 kb on - strand, within BT0954 at 1165.063 kb on - strand, within BT0954 at 1165.114 kb on - strand, within BT0954 at 1165.278 kb on + strand, within BT0954 at 1165.429 kb on - strand, within BT0954 at 1165.429 kb on - strand, within BT0954 at 1165.604 kb on - strand at 1165.607 kb on - strand at 1165.613 kb on - strand at 1165.738 kb on - strand at 1165.779 kb on - strand at 1165.908 kb on + strand, within BT0955 at 1166.001 kb on - strand, within BT0955 at 1166.046 kb on - strand, within BT0955 at 1166.070 kb on + strand, within BT0955 at 1166.089 kb on + strand, within BT0955 at 1166.103 kb on + strand, within BT0955 at 1166.111 kb on - strand, within BT0955 at 1166.158 kb on - strand, within BT0955 at 1166.158 kb on - strand, within BT0955 at 1166.194 kb on + strand, within BT0955 at 1166.257 kb on - strand, within BT0955 at 1166.343 kb on - strand, within BT0955 at 1166.591 kb on - strand at 1166.598 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM remove 1,163,286 + +0.2 1,163,286 + -1.4 1,163,287 - -0.8 1,163,315 + -2.1 1,163,322 + +1.4 1,163,334 - -0.8 1,163,334 - +0.2 1,163,334 - +0.2 1,163,443 - -2.8 1,163,507 + tRNA-Gly BT_t18 0.60 -2.6 1,163,539 - -0.8 1,163,545 - -0.8 1,163,640 - -2.8 1,163,645 - -0.8 1,163,646 + +0.2 1,163,843 + -0.8 1,163,864 - -2.8 1,163,908 + -2.4 1,163,921 + -2.1 1,163,943 - -2.6 1,164,068 + -2.1 1,164,070 + -0.8 1,164,071 - +0.2 1,164,103 - -3.3 1,164,144 - -0.8 1,164,172 + +2.2 1,164,264 + +0.2 1,164,270 - -1.8 1,164,543 + BT0954 0.19 -1.8 1,164,566 + BT0954 0.20 -0.8 1,164,593 + BT0954 0.22 -1.4 1,164,618 - BT0954 0.24 +0.2 1,164,809 - BT0954 0.38 -5.1 1,165,063 - BT0954 0.56 -0.9 1,165,114 - BT0954 0.59 -1.4 1,165,278 + BT0954 0.71 -0.8 1,165,429 - BT0954 0.82 -1.1 1,165,429 - BT0954 0.82 -1.4 1,165,604 - -1.8 1,165,607 - -0.8 1,165,613 - +0.8 1,165,738 - +0.2 1,165,779 - +0.9 1,165,908 + BT0955 0.21 -3.7 1,166,001 - BT0955 0.31 -1.8 1,166,046 - BT0955 0.35 -2.1 1,166,070 + BT0955 0.38 -2.1 1,166,089 + BT0955 0.40 +1.4 1,166,103 + BT0955 0.41 -0.8 1,166,111 - BT0955 0.42 +0.2 1,166,158 - BT0955 0.47 -3.5 1,166,158 - BT0955 0.47 +2.2 1,166,194 + BT0955 0.50 -1.1 1,166,257 - BT0955 0.57 +0.2 1,166,343 - BT0955 0.65 +0.2 1,166,591 - -2.8 1,166,598 - -1.4
Or see this region's nucleotide sequence