Experiment: Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0615 and BT0616 are separated by 357 nucleotides BT0616 and BT0617 are separated by 6 nucleotides BT0617 and BT0618 are separated by 24 nucleotides
BT0615: BT0615 - conserved hypothetical protein (NCBI ptt file), at 760,881 to 761,399
BT0615
BT0616: BT0616 - conserved hypothetical protein (NCBI ptt file), at 761,757 to 762,182
BT0616
BT0617: BT0617 - Na+-transporting NADH:ubiquinone oxidoreductase, Electron transport complex protein rnfB (NCBI ptt file), at 762,189 to 763,070
BT0617
BT0618: BT0618 - Na+-transporting NADH:ubiquinone oxidoreductase, Electron transport complex protein rnfC (NCBI ptt file), at 763,095 to 764,432
BT0618
Position (kb)
762
763
764 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 761.220 kb on - strand, within BT0615 at 761.525 kb on - strand at 761.538 kb on + strand at 761.547 kb on + strand at 761.547 kb on + strand at 761.560 kb on - strand at 761.601 kb on + strand at 761.602 kb on - strand at 761.629 kb on - strand at 761.771 kb on + strand at 761.772 kb on - strand at 761.783 kb on - strand at 761.906 kb on + strand, within BT0616 at 761.906 kb on + strand, within BT0616 at 761.939 kb on - strand, within BT0616 at 762.004 kb on + strand, within BT0616 at 762.070 kb on - strand, within BT0616 at 762.116 kb on - strand, within BT0616 at 762.228 kb on - strand at 762.497 kb on - strand, within BT0617 at 762.583 kb on - strand, within BT0617 at 762.708 kb on - strand, within BT0617 at 762.790 kb on - strand, within BT0617 at 763.161 kb on - strand at 763.603 kb on - strand, within BT0618 at 763.654 kb on + strand, within BT0618 at 763.688 kb on - strand, within BT0618
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM remove 761,220 - BT0615 0.65 -2.2 761,525 - -0.8 761,538 + -0.8 761,547 + -1.1 761,547 + -1.0 761,560 - -1.8 761,601 + +0.2 761,602 - -2.9 761,629 - -1.1 761,771 + -3.2 761,772 - -1.8 761,783 - -0.8 761,906 + BT0616 0.35 -0.8 761,906 + BT0616 0.35 -0.8 761,939 - BT0616 0.43 -1.4 762,004 + BT0616 0.58 -1.4 762,070 - BT0616 0.73 -0.8 762,116 - BT0616 0.84 -2.8 762,228 - -0.8 762,497 - BT0617 0.35 -0.8 762,583 - BT0617 0.45 -0.8 762,708 - BT0617 0.59 -1.4 762,790 - BT0617 0.68 -1.8 763,161 - +0.2 763,603 - BT0618 0.38 +0.2 763,654 + BT0618 0.42 -2.2 763,688 - BT0618 0.44 -1.4
Or see this region's nucleotide sequence