Experiment: Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0201 and BT0202 overlap by 4 nucleotides BT0202 and BT0203 are separated by 3 nucleotides
BT0201: BT0201 - histidinol dehydrogenase (NCBI ptt file), at 215,955 to 217,241
BT0201
BT0202: BT0202 - histidinol-phosphate aminotransferase (EC 2.6.1.9) <small>(from data)</small>, at 217,238 to 218,278
BT0202
BT0203: BT0203 - histidine biosynthesis bifunctional protein hisB (NCBI ptt file), at 218,282 to 219,406
BT0203
Position (kb) 217
218
219 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 216.309 kb on - strand, within BT0201 at 216.330 kb on - strand, within BT0201 at 216.455 kb on + strand, within BT0201 at 216.455 kb on - strand, within BT0201 at 216.494 kb on - strand, within BT0201 at 216.494 kb on - strand, within BT0201 at 216.494 kb on - strand, within BT0201 at 216.534 kb on + strand, within BT0201 at 216.535 kb on - strand, within BT0201 at 216.629 kb on - strand, within BT0201 at 216.713 kb on - strand, within BT0201 at 216.787 kb on + strand, within BT0201 at 216.799 kb on + strand, within BT0201 at 216.800 kb on - strand, within BT0201 at 217.046 kb on + strand, within BT0201 at 217.047 kb on - strand, within BT0201 at 217.047 kb on - strand, within BT0201 at 217.230 kb on - strand at 217.271 kb on - strand at 217.282 kb on - strand at 217.311 kb on - strand at 217.313 kb on - strand at 217.361 kb on - strand, within BT0202 at 217.447 kb on + strand, within BT0202 at 217.448 kb on - strand, within BT0202 at 217.524 kb on + strand, within BT0202 at 217.524 kb on + strand, within BT0202 at 217.524 kb on + strand, within BT0202 at 217.525 kb on - strand, within BT0202 at 217.545 kb on - strand, within BT0202 at 217.554 kb on - strand, within BT0202 at 217.658 kb on - strand, within BT0202 at 217.731 kb on + strand, within BT0202 at 217.773 kb on - strand, within BT0202 at 217.775 kb on - strand, within BT0202 at 217.941 kb on - strand, within BT0202 at 217.941 kb on - strand, within BT0202 at 217.941 kb on - strand, within BT0202 at 217.992 kb on - strand, within BT0202 at 217.995 kb on - strand, within BT0202 at 218.084 kb on + strand, within BT0202 at 218.085 kb on - strand, within BT0202 at 218.277 kb on - strand at 218.281 kb on + strand at 218.281 kb on + strand at 218.282 kb on - strand at 218.309 kb on - strand at 218.346 kb on - strand at 218.627 kb on - strand, within BT0203 at 218.627 kb on - strand, within BT0203 at 218.870 kb on - strand, within BT0203 at 218.873 kb on + strand, within BT0203 at 218.874 kb on - strand, within BT0203 at 218.874 kb on - strand, within BT0203 at 219.058 kb on - strand, within BT0203 at 219.116 kb on - strand, within BT0203 at 219.172 kb on - strand, within BT0203
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Rifampicin 0.0001953125 mM remove 216,309 - BT0201 0.28 -1.4 216,330 - BT0201 0.29 -3.3 216,455 + BT0201 0.39 -4.0 216,455 - BT0201 0.39 -1.4 216,494 - BT0201 0.42 -2.6 216,494 - BT0201 0.42 -0.8 216,494 - BT0201 0.42 +0.2 216,534 + BT0201 0.45 -2.6 216,535 - BT0201 0.45 -2.2 216,629 - BT0201 0.52 -1.4 216,713 - BT0201 0.59 -0.8 216,787 + BT0201 0.65 -2.0 216,799 + BT0201 0.66 -2.8 216,800 - BT0201 0.66 -2.6 217,046 + BT0201 0.85 -2.8 217,047 - BT0201 0.85 -0.8 217,047 - BT0201 0.85 -2.6 217,230 - -2.2 217,271 - -2.2 217,282 - -2.2 217,311 - -0.6 217,313 - +0.1 217,361 - BT0202 0.12 -1.4 217,447 + BT0202 0.20 -3.0 217,448 - BT0202 0.20 -1.9 217,524 + BT0202 0.27 -1.4 217,524 + BT0202 0.27 -0.9 217,524 + BT0202 0.27 -2.0 217,525 - BT0202 0.28 -3.2 217,545 - BT0202 0.29 -0.9 217,554 - BT0202 0.30 -4.5 217,658 - BT0202 0.40 -1.0 217,731 + BT0202 0.47 -1.4 217,773 - BT0202 0.51 -2.4 217,775 - BT0202 0.52 -3.2 217,941 - BT0202 0.68 -1.9 217,941 - BT0202 0.68 -5.3 217,941 - BT0202 0.68 -2.2 217,992 - BT0202 0.72 -3.6 217,995 - BT0202 0.73 -1.9 218,084 + BT0202 0.81 -3.0 218,085 - BT0202 0.81 -3.0 218,277 - -3.8 218,281 + -2.2 218,281 + +0.1 218,282 - -1.2 218,309 - -4.9 218,346 - -5.4 218,627 - BT0203 0.31 -4.4 218,627 - BT0203 0.31 -1.4 218,870 - BT0203 0.52 -2.2 218,873 + BT0203 0.53 -1.4 218,874 - BT0203 0.53 -2.2 218,874 - BT0203 0.53 +0.1 219,058 - BT0203 0.69 +0.1 219,116 - BT0203 0.74 -1.9 219,172 - BT0203 0.79 -3.0
Or see this region's nucleotide sequence