Experiment: RCH2 defined with Cobalt chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_812 and Pf6N2E2_813 are separated by 77 nucleotides Pf6N2E2_813 and Pf6N2E2_814 are separated by 212 nucleotides Pf6N2E2_814 and Pf6N2E2_815 overlap by 30 nucleotides
Pf6N2E2_812 - Glycosyltransferase, at 944,886 to 946,178
_812
Pf6N2E2_813 - Response regulator, at 946,256 to 946,561
_813
Pf6N2E2_814 - hypothetical protein, at 946,774 to 946,917
_814
Pf6N2E2_815 - Signal transduction histidine kinase, at 946,888 to 950,370
_815
Position (kb) 946
947 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 946.138 kb on + strand at 946.138 kb on + strand at 946.138 kb on + strand at 946.138 kb on + strand at 946.139 kb on - strand at 946.342 kb on + strand, within Pf6N2E2_813 at 946.343 kb on - strand, within Pf6N2E2_813 at 946.527 kb on + strand, within Pf6N2E2_813 at 946.527 kb on + strand, within Pf6N2E2_813 at 946.527 kb on + strand, within Pf6N2E2_813 at 946.527 kb on + strand, within Pf6N2E2_813 at 946.527 kb on + strand, within Pf6N2E2_813 at 946.627 kb on + strand at 946.628 kb on - strand at 946.628 kb on - strand at 946.628 kb on - strand at 946.628 kb on - strand at 946.628 kb on - strand at 946.628 kb on - strand at 946.628 kb on - strand at 946.690 kb on + strand at 946.690 kb on + strand at 946.690 kb on + strand at 946.691 kb on - strand at 946.724 kb on - strand at 946.737 kb on + strand at 946.737 kb on + strand at 946.738 kb on - strand at 946.739 kb on + strand at 946.740 kb on - strand at 946.742 kb on + strand at 946.775 kb on + strand at 946.776 kb on - strand at 946.782 kb on + strand at 946.819 kb on + strand, within Pf6N2E2_814 at 946.819 kb on + strand, within Pf6N2E2_814 at 946.819 kb on + strand, within Pf6N2E2_814 at 946.819 kb on + strand, within Pf6N2E2_814 at 946.820 kb on - strand, within Pf6N2E2_814 at 946.820 kb on - strand, within Pf6N2E2_814 at 946.820 kb on - strand, within Pf6N2E2_814 at 946.820 kb on - strand, within Pf6N2E2_814 at 946.944 kb on + strand at 946.944 kb on + strand at 946.944 kb on + strand at 947.005 kb on + strand at 947.005 kb on + strand at 947.005 kb on + strand at 947.006 kb on - strand at 947.146 kb on + strand at 947.184 kb on + strand at 947.185 kb on - strand at 947.185 kb on - strand at 947.240 kb on + strand, within Pf6N2E2_815 at 947.240 kb on + strand, within Pf6N2E2_815 at 947.240 kb on + strand, within Pf6N2E2_815 at 947.241 kb on - strand, within Pf6N2E2_815 at 947.241 kb on - strand, within Pf6N2E2_815 at 947.241 kb on - strand, within Pf6N2E2_815 at 947.241 kb on - strand, within Pf6N2E2_815 at 947.270 kb on - strand, within Pf6N2E2_815 at 947.270 kb on - strand, within Pf6N2E2_815 at 947.458 kb on + strand, within Pf6N2E2_815 at 947.458 kb on + strand, within Pf6N2E2_815 at 947.459 kb on - strand, within Pf6N2E2_815 at 947.497 kb on + strand, within Pf6N2E2_815 at 947.498 kb on - strand, within Pf6N2E2_815 at 947.498 kb on - strand, within Pf6N2E2_815 at 947.536 kb on + strand, within Pf6N2E2_815 at 947.536 kb on + strand, within Pf6N2E2_815 at 947.537 kb on - strand, within Pf6N2E2_815 at 947.537 kb on - strand, within Pf6N2E2_815 at 947.537 kb on - strand, within Pf6N2E2_815 at 947.537 kb on - strand, within Pf6N2E2_815 at 947.668 kb on - strand, within Pf6N2E2_815 at 947.668 kb on - strand, within Pf6N2E2_815 at 947.689 kb on + strand, within Pf6N2E2_815 at 947.690 kb on - strand, within Pf6N2E2_815 at 947.716 kb on + strand, within Pf6N2E2_815 at 947.758 kb on + strand, within Pf6N2E2_815 at 947.758 kb on + strand, within Pf6N2E2_815 at 947.759 kb on - strand, within Pf6N2E2_815 at 947.759 kb on - strand, within Pf6N2E2_815 at 947.814 kb on + strand, within Pf6N2E2_815 at 947.815 kb on - strand, within Pf6N2E2_815 at 947.864 kb on + strand, within Pf6N2E2_815
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.02 mM remove 946,138 + -0.4 946,138 + -0.2 946,138 + -1.4 946,138 + -2.4 946,139 - +1.2 946,342 + Pf6N2E2_813 0.28 +0.2 946,343 - Pf6N2E2_813 0.28 -1.2 946,527 + Pf6N2E2_813 0.89 -1.4 946,527 + Pf6N2E2_813 0.89 +0.7 946,527 + Pf6N2E2_813 0.89 -1.8 946,527 + Pf6N2E2_813 0.89 -1.2 946,527 + Pf6N2E2_813 0.89 +0.3 946,627 + +0.2 946,628 - -1.8 946,628 - +0.2 946,628 - +0.2 946,628 - +0.2 946,628 - -1.2 946,628 - -2.2 946,628 - -0.2 946,690 + -0.1 946,690 + +0.2 946,690 + +0.8 946,691 - +0.2 946,724 - -0.8 946,737 + +0.2 946,737 + -2.2 946,738 - +1.2 946,739 + +0.2 946,740 - +1.2 946,742 + -1.8 946,775 + -2.2 946,776 - -0.6 946,782 + -2.4 946,819 + Pf6N2E2_814 0.31 +0.2 946,819 + Pf6N2E2_814 0.31 -0.8 946,819 + Pf6N2E2_814 0.31 -0.6 946,819 + Pf6N2E2_814 0.31 -1.2 946,820 - Pf6N2E2_814 0.32 -3.0 946,820 - Pf6N2E2_814 0.32 -1.5 946,820 - Pf6N2E2_814 0.32 -3.5 946,820 - Pf6N2E2_814 0.32 -0.5 946,944 + -1.8 946,944 + +0.2 946,944 + -0.8 947,005 + +0.2 947,005 + +0.2 947,005 + -2.4 947,006 - +1.2 947,146 + -1.4 947,184 + -0.8 947,185 - -0.8 947,185 - +0.4 947,240 + Pf6N2E2_815 0.10 -1.8 947,240 + Pf6N2E2_815 0.10 +0.2 947,240 + Pf6N2E2_815 0.10 +0.2 947,241 - Pf6N2E2_815 0.10 -1.1 947,241 - Pf6N2E2_815 0.10 -0.5 947,241 - Pf6N2E2_815 0.10 -0.8 947,241 - Pf6N2E2_815 0.10 -0.8 947,270 - Pf6N2E2_815 0.11 +0.2 947,270 - Pf6N2E2_815 0.11 -0.8 947,458 + Pf6N2E2_815 0.16 -1.4 947,458 + Pf6N2E2_815 0.16 +0.3 947,459 - Pf6N2E2_815 0.16 -1.8 947,497 + Pf6N2E2_815 0.17 -0.8 947,498 - Pf6N2E2_815 0.18 -1.8 947,498 - Pf6N2E2_815 0.18 +0.2 947,536 + Pf6N2E2_815 0.19 -0.8 947,536 + Pf6N2E2_815 0.19 +0.2 947,537 - Pf6N2E2_815 0.19 -0.8 947,537 - Pf6N2E2_815 0.19 -0.8 947,537 - Pf6N2E2_815 0.19 +0.2 947,537 - Pf6N2E2_815 0.19 +0.2 947,668 - Pf6N2E2_815 0.22 -0.8 947,668 - Pf6N2E2_815 0.22 +0.2 947,689 + Pf6N2E2_815 0.23 -1.4 947,690 - Pf6N2E2_815 0.23 -0.8 947,716 + Pf6N2E2_815 0.24 +1.2 947,758 + Pf6N2E2_815 0.25 -0.4 947,758 + Pf6N2E2_815 0.25 +0.2 947,759 - Pf6N2E2_815 0.25 -2.4 947,759 - Pf6N2E2_815 0.25 -1.4 947,814 + Pf6N2E2_815 0.27 +0.2 947,815 - Pf6N2E2_815 0.27 -0.8 947,864 + Pf6N2E2_815 0.28 -0.2
Or see this region's nucleotide sequence