Experiment: RCH2 defined with Cobalt chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2349 and Pf6N2E2_2350 are separated by 4 nucleotides Pf6N2E2_2350 and Pf6N2E2_2351 overlap by 29 nucleotides Pf6N2E2_2351 and Pf6N2E2_2352 are separated by 4 nucleotides Pf6N2E2_2352 and Pf6N2E2_2353 overlap by 4 nucleotides
Pf6N2E2_2349 - hypothetical protein, at 2,785,576 to 2,785,989
_2349
Pf6N2E2_2350 - Oxygen-regulated invasion protein OrgB, at 2,785,994 to 2,786,704
_2350
Pf6N2E2_2351 - Oxygen-regulated invasion protein OrgA, at 2,786,676 to 2,787,224
_2351
Pf6N2E2_2352 - Type III secretion bridge between inner and outermembrane lipoprotein (YscJ,HrcJ,EscJ, PscJ), at 2,787,229 to 2,787,948
_2352
Pf6N2E2_2353 - cell invasion protein - cytoplasmic, at 2,787,945 to 2,788,253
_2353
Position (kb)
2786
2787
2788 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2785.693 kb on - strand, within Pf6N2E2_2349 at 2785.694 kb on + strand, within Pf6N2E2_2349 at 2785.694 kb on + strand, within Pf6N2E2_2349 at 2785.694 kb on + strand, within Pf6N2E2_2349 at 2785.694 kb on + strand, within Pf6N2E2_2349 at 2785.694 kb on + strand, within Pf6N2E2_2349 at 2785.694 kb on + strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.738 kb on + strand, within Pf6N2E2_2349 at 2785.739 kb on - strand, within Pf6N2E2_2349 at 2785.739 kb on - strand, within Pf6N2E2_2349 at 2785.871 kb on + strand, within Pf6N2E2_2349 at 2785.871 kb on + strand, within Pf6N2E2_2349 at 2785.871 kb on + strand, within Pf6N2E2_2349 at 2785.871 kb on + strand, within Pf6N2E2_2349 at 2786.205 kb on + strand, within Pf6N2E2_2350 at 2786.205 kb on + strand, within Pf6N2E2_2350 at 2786.206 kb on - strand, within Pf6N2E2_2350 at 2786.223 kb on + strand, within Pf6N2E2_2350 at 2786.224 kb on - strand, within Pf6N2E2_2350 at 2786.224 kb on - strand, within Pf6N2E2_2350 at 2786.920 kb on - strand, within Pf6N2E2_2351 at 2787.023 kb on + strand, within Pf6N2E2_2351 at 2787.023 kb on + strand, within Pf6N2E2_2351 at 2787.024 kb on - strand, within Pf6N2E2_2351 at 2787.024 kb on - strand, within Pf6N2E2_2351 at 2787.026 kb on - strand, within Pf6N2E2_2351 at 2787.026 kb on - strand, within Pf6N2E2_2351 at 2787.026 kb on - strand, within Pf6N2E2_2351 at 2787.132 kb on + strand, within Pf6N2E2_2351 at 2787.133 kb on - strand, within Pf6N2E2_2351 at 2787.133 kb on - strand, within Pf6N2E2_2351 at 2787.193 kb on + strand at 2787.194 kb on - strand at 2787.269 kb on + strand at 2787.413 kb on + strand, within Pf6N2E2_2352 at 2787.557 kb on + strand, within Pf6N2E2_2352 at 2787.558 kb on - strand, within Pf6N2E2_2352 at 2787.635 kb on + strand, within Pf6N2E2_2352 at 2787.636 kb on - strand, within Pf6N2E2_2352 at 2788.024 kb on + strand, within Pf6N2E2_2353 at 2788.024 kb on + strand, within Pf6N2E2_2353 at 2788.024 kb on + strand, within Pf6N2E2_2353 at 2788.025 kb on - strand, within Pf6N2E2_2353 at 2788.025 kb on - strand, within Pf6N2E2_2353 at 2788.056 kb on + strand, within Pf6N2E2_2353 at 2788.056 kb on + strand, within Pf6N2E2_2353 at 2788.056 kb on + strand, within Pf6N2E2_2353 at 2788.056 kb on + strand, within Pf6N2E2_2353 at 2788.057 kb on - strand, within Pf6N2E2_2353 at 2788.057 kb on - strand, within Pf6N2E2_2353
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.02 mM remove 2,785,693 - Pf6N2E2_2349 0.28 -3.5 2,785,694 + Pf6N2E2_2349 0.29 -0.6 2,785,694 + Pf6N2E2_2349 0.29 -0.3 2,785,694 + Pf6N2E2_2349 0.29 +0.3 2,785,694 + Pf6N2E2_2349 0.29 -3.7 2,785,694 + Pf6N2E2_2349 0.29 +0.8 2,785,694 + Pf6N2E2_2349 0.29 -0.2 2,785,695 - Pf6N2E2_2349 0.29 -0.7 2,785,695 - Pf6N2E2_2349 0.29 +0.5 2,785,695 - Pf6N2E2_2349 0.29 +0.3 2,785,695 - Pf6N2E2_2349 0.29 -0.2 2,785,695 - Pf6N2E2_2349 0.29 -0.5 2,785,695 - Pf6N2E2_2349 0.29 +0.2 2,785,695 - Pf6N2E2_2349 0.29 -2.5 2,785,695 - Pf6N2E2_2349 0.29 -0.4 2,785,695 - Pf6N2E2_2349 0.29 -1.1 2,785,738 + Pf6N2E2_2349 0.39 -0.0 2,785,739 - Pf6N2E2_2349 0.39 -1.5 2,785,739 - Pf6N2E2_2349 0.39 +0.3 2,785,871 + Pf6N2E2_2349 0.71 +0.5 2,785,871 + Pf6N2E2_2349 0.71 +1.9 2,785,871 + Pf6N2E2_2349 0.71 +2.5 2,785,871 + Pf6N2E2_2349 0.71 -0.6 2,786,205 + Pf6N2E2_2350 0.30 -0.2 2,786,205 + Pf6N2E2_2350 0.30 -0.9 2,786,206 - Pf6N2E2_2350 0.30 -1.9 2,786,223 + Pf6N2E2_2350 0.32 -0.1 2,786,224 - Pf6N2E2_2350 0.32 +0.5 2,786,224 - Pf6N2E2_2350 0.32 -1.1 2,786,920 - Pf6N2E2_2351 0.44 +0.8 2,787,023 + Pf6N2E2_2351 0.63 +1.5 2,787,023 + Pf6N2E2_2351 0.63 -1.9 2,787,024 - Pf6N2E2_2351 0.63 +1.2 2,787,024 - Pf6N2E2_2351 0.63 +1.7 2,787,026 - Pf6N2E2_2351 0.64 -0.3 2,787,026 - Pf6N2E2_2351 0.64 +0.0 2,787,026 - Pf6N2E2_2351 0.64 +1.3 2,787,132 + Pf6N2E2_2351 0.83 +0.5 2,787,133 - Pf6N2E2_2351 0.83 +0.3 2,787,133 - Pf6N2E2_2351 0.83 +0.3 2,787,193 + -0.7 2,787,194 - -0.2 2,787,269 + +0.5 2,787,413 + Pf6N2E2_2352 0.26 -1.5 2,787,557 + Pf6N2E2_2352 0.46 -0.9 2,787,558 - Pf6N2E2_2352 0.46 -0.5 2,787,635 + Pf6N2E2_2352 0.56 -0.4 2,787,636 - Pf6N2E2_2352 0.57 +0.3 2,788,024 + Pf6N2E2_2353 0.26 -1.5 2,788,024 + Pf6N2E2_2353 0.26 -1.9 2,788,024 + Pf6N2E2_2353 0.26 -0.2 2,788,025 - Pf6N2E2_2353 0.26 +0.5 2,788,025 - Pf6N2E2_2353 0.26 +1.1 2,788,056 + Pf6N2E2_2353 0.36 -0.1 2,788,056 + Pf6N2E2_2353 0.36 +0.1 2,788,056 + Pf6N2E2_2353 0.36 -0.9 2,788,056 + Pf6N2E2_2353 0.36 -0.5 2,788,057 - Pf6N2E2_2353 0.36 -1.5 2,788,057 - Pf6N2E2_2353 0.36 -1.1
Or see this region's nucleotide sequence