Experiment: RCH2 defined with Cobalt chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2181 and Pf6N2E2_2182 are separated by 133 nucleotides Pf6N2E2_2182 and Pf6N2E2_2183 overlap by 4 nucleotides Pf6N2E2_2183 and Pf6N2E2_2184 are separated by 26 nucleotides Pf6N2E2_2184 and Pf6N2E2_2185 are separated by 177 nucleotides
Pf6N2E2_2181 - Arginine N-succinyltransferase (EC 2.3.1.109), at 2,586,219 to 2,587,253
_2181
Pf6N2E2_2182 - hypothetical protein, at 2,587,387 to 2,588,166
_2182
Pf6N2E2_2183 - hypothetical protein, at 2,588,163 to 2,588,825
_2183
Pf6N2E2_2184 - hypothetical protein, at 2,588,852 to 2,589,031
_2184
Pf6N2E2_2185 - carboxyphosphonoenolpyruvate phosphonomutase, putative, at 2,589,209 to 2,590,078
_2185
Position (kb)
2588
2589 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2587.285 kb on - strand at 2587.285 kb on - strand at 2587.289 kb on - strand at 2587.289 kb on - strand at 2587.289 kb on - strand at 2587.329 kb on - strand at 2587.329 kb on - strand at 2587.357 kb on - strand at 2587.434 kb on + strand at 2587.457 kb on + strand at 2587.652 kb on - strand, within Pf6N2E2_2182 at 2587.671 kb on + strand, within Pf6N2E2_2182 at 2587.712 kb on - strand, within Pf6N2E2_2182 at 2587.884 kb on - strand, within Pf6N2E2_2182 at 2587.884 kb on - strand, within Pf6N2E2_2182 at 2587.884 kb on - strand, within Pf6N2E2_2182 at 2588.017 kb on + strand, within Pf6N2E2_2182 at 2588.017 kb on + strand, within Pf6N2E2_2182 at 2588.018 kb on - strand, within Pf6N2E2_2182 at 2588.024 kb on - strand, within Pf6N2E2_2182 at 2588.092 kb on + strand at 2588.093 kb on - strand at 2588.093 kb on - strand at 2588.199 kb on + strand at 2588.199 kb on + strand at 2588.200 kb on - strand at 2588.200 kb on - strand at 2588.283 kb on + strand, within Pf6N2E2_2183 at 2588.284 kb on - strand, within Pf6N2E2_2183 at 2588.286 kb on - strand, within Pf6N2E2_2183 at 2588.328 kb on + strand, within Pf6N2E2_2183 at 2588.329 kb on - strand, within Pf6N2E2_2183 at 2588.338 kb on - strand, within Pf6N2E2_2183 at 2588.490 kb on + strand, within Pf6N2E2_2183 at 2588.503 kb on + strand, within Pf6N2E2_2183 at 2588.525 kb on - strand, within Pf6N2E2_2183 at 2588.574 kb on - strand, within Pf6N2E2_2183 at 2588.597 kb on - strand, within Pf6N2E2_2183 at 2588.597 kb on - strand, within Pf6N2E2_2183 at 2588.598 kb on + strand, within Pf6N2E2_2183 at 2588.598 kb on + strand, within Pf6N2E2_2183 at 2588.598 kb on + strand, within Pf6N2E2_2183 at 2588.598 kb on + strand, within Pf6N2E2_2183 at 2588.598 kb on + strand, within Pf6N2E2_2183 at 2588.598 kb on + strand, within Pf6N2E2_2183 at 2588.598 kb on + strand, within Pf6N2E2_2183 at 2588.599 kb on - strand, within Pf6N2E2_2183 at 2588.599 kb on - strand, within Pf6N2E2_2183 at 2588.599 kb on - strand, within Pf6N2E2_2183 at 2588.599 kb on - strand, within Pf6N2E2_2183 at 2588.599 kb on - strand, within Pf6N2E2_2183 at 2588.724 kb on + strand, within Pf6N2E2_2183 at 2588.724 kb on + strand, within Pf6N2E2_2183 at 2588.724 kb on + strand, within Pf6N2E2_2183 at 2588.724 kb on + strand, within Pf6N2E2_2183 at 2588.725 kb on - strand, within Pf6N2E2_2183 at 2588.725 kb on - strand, within Pf6N2E2_2183 at 2588.725 kb on - strand, within Pf6N2E2_2183 at 2588.725 kb on - strand, within Pf6N2E2_2183 at 2588.728 kb on + strand, within Pf6N2E2_2183 at 2588.729 kb on - strand, within Pf6N2E2_2183 at 2588.729 kb on - strand, within Pf6N2E2_2183 at 2588.746 kb on + strand, within Pf6N2E2_2183 at 2588.782 kb on + strand at 2588.782 kb on + strand at 2588.782 kb on + strand at 2588.782 kb on + strand at 2588.782 kb on + strand at 2588.783 kb on - strand at 2588.783 kb on - strand at 2588.850 kb on + strand at 2588.850 kb on + strand at 2589.021 kb on + strand at 2589.022 kb on - strand at 2589.022 kb on - strand at 2589.022 kb on - strand at 2589.024 kb on + strand at 2589.024 kb on + strand at 2589.025 kb on - strand at 2589.025 kb on - strand at 2589.241 kb on + strand at 2589.275 kb on + strand at 2589.276 kb on - strand at 2589.339 kb on + strand, within Pf6N2E2_2185 at 2589.339 kb on + strand, within Pf6N2E2_2185 at 2589.339 kb on + strand, within Pf6N2E2_2185 at 2589.339 kb on + strand, within Pf6N2E2_2185 at 2589.340 kb on - strand, within Pf6N2E2_2185 at 2589.486 kb on - strand, within Pf6N2E2_2185 at 2589.737 kb on + strand, within Pf6N2E2_2185 at 2589.738 kb on - strand, within Pf6N2E2_2185 at 2589.738 kb on - strand, within Pf6N2E2_2185 at 2589.738 kb on - strand, within Pf6N2E2_2185 at 2589.738 kb on - strand, within Pf6N2E2_2185 at 2589.738 kb on - strand, within Pf6N2E2_2185 at 2589.738 kb on - strand, within Pf6N2E2_2185
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.02 mM remove 2,587,285 - +0.4 2,587,285 - +0.4 2,587,289 - +0.0 2,587,289 - -0.5 2,587,289 - -0.4 2,587,329 - +2.7 2,587,329 - -2.8 2,587,357 - -0.6 2,587,434 + -2.2 2,587,457 + +0.1 2,587,652 - Pf6N2E2_2182 0.34 -0.4 2,587,671 + Pf6N2E2_2182 0.36 -1.2 2,587,712 - Pf6N2E2_2182 0.42 +0.4 2,587,884 - Pf6N2E2_2182 0.64 +0.5 2,587,884 - Pf6N2E2_2182 0.64 +1.1 2,587,884 - Pf6N2E2_2182 0.64 -0.3 2,588,017 + Pf6N2E2_2182 0.81 -2.2 2,588,017 + Pf6N2E2_2182 0.81 -1.2 2,588,018 - Pf6N2E2_2182 0.81 -0.8 2,588,024 - Pf6N2E2_2182 0.82 -0.4 2,588,092 + -0.6 2,588,093 - +0.7 2,588,093 - +1.0 2,588,199 + -0.1 2,588,199 + -1.2 2,588,200 - +0.4 2,588,200 - +0.1 2,588,283 + Pf6N2E2_2183 0.18 +1.2 2,588,284 - Pf6N2E2_2183 0.18 -0.9 2,588,286 - Pf6N2E2_2183 0.19 -1.2 2,588,328 + Pf6N2E2_2183 0.25 +1.8 2,588,329 - Pf6N2E2_2183 0.25 +0.4 2,588,338 - Pf6N2E2_2183 0.26 -1.6 2,588,490 + Pf6N2E2_2183 0.49 -2.1 2,588,503 + Pf6N2E2_2183 0.51 -1.6 2,588,525 - Pf6N2E2_2183 0.55 -0.6 2,588,574 - Pf6N2E2_2183 0.62 -1.2 2,588,597 - Pf6N2E2_2183 0.65 +1.3 2,588,597 - Pf6N2E2_2183 0.65 -0.7 2,588,598 + Pf6N2E2_2183 0.66 +2.4 2,588,598 + Pf6N2E2_2183 0.66 +0.6 2,588,598 + Pf6N2E2_2183 0.66 -1.2 2,588,598 + Pf6N2E2_2183 0.66 -0.9 2,588,598 + Pf6N2E2_2183 0.66 +0.1 2,588,598 + Pf6N2E2_2183 0.66 +0.9 2,588,598 + Pf6N2E2_2183 0.66 +0.4 2,588,599 - Pf6N2E2_2183 0.66 +1.3 2,588,599 - Pf6N2E2_2183 0.66 +0.0 2,588,599 - Pf6N2E2_2183 0.66 -0.1 2,588,599 - Pf6N2E2_2183 0.66 +0.4 2,588,599 - Pf6N2E2_2183 0.66 -0.9 2,588,724 + Pf6N2E2_2183 0.85 -0.3 2,588,724 + Pf6N2E2_2183 0.85 -0.6 2,588,724 + Pf6N2E2_2183 0.85 -0.1 2,588,724 + Pf6N2E2_2183 0.85 -0.2 2,588,725 - Pf6N2E2_2183 0.85 -0.3 2,588,725 - Pf6N2E2_2183 0.85 -1.0 2,588,725 - Pf6N2E2_2183 0.85 -2.2 2,588,725 - Pf6N2E2_2183 0.85 -0.6 2,588,728 + Pf6N2E2_2183 0.85 -0.6 2,588,729 - Pf6N2E2_2183 0.85 -0.0 2,588,729 - Pf6N2E2_2183 0.85 -0.9 2,588,746 + Pf6N2E2_2183 0.88 +0.6 2,588,782 + -0.6 2,588,782 + -0.6 2,588,782 + +0.4 2,588,782 + +0.4 2,588,782 + -0.1 2,588,783 - +0.4 2,588,783 - -0.1 2,588,850 + +0.4 2,588,850 + +0.4 2,589,021 + +1.8 2,589,022 - -0.6 2,589,022 - -1.0 2,589,022 - -0.5 2,589,024 + -0.5 2,589,024 + -0.0 2,589,025 - -1.6 2,589,025 - -0.6 2,589,241 + +0.6 2,589,275 + -0.6 2,589,276 - +1.1 2,589,339 + Pf6N2E2_2185 0.15 +1.1 2,589,339 + Pf6N2E2_2185 0.15 +0.8 2,589,339 + Pf6N2E2_2185 0.15 +0.4 2,589,339 + Pf6N2E2_2185 0.15 +0.8 2,589,340 - Pf6N2E2_2185 0.15 +0.0 2,589,486 - Pf6N2E2_2185 0.32 -1.3 2,589,737 + Pf6N2E2_2185 0.61 -0.5 2,589,738 - Pf6N2E2_2185 0.61 +0.4 2,589,738 - Pf6N2E2_2185 0.61 -4.2 2,589,738 - Pf6N2E2_2185 0.61 -0.1 2,589,738 - Pf6N2E2_2185 0.61 +3.0 2,589,738 - Pf6N2E2_2185 0.61 +0.0 2,589,738 - Pf6N2E2_2185 0.61 -0.1
Or see this region's nucleotide sequence