Strain Fitness in Ralstonia sp. UNC404CL21Col around ABZR87_RS17470

Experiment: nitrogen source nitrite 0.5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABZR87_RS17465 and ABZR87_RS17470 are separated by 72 nucleotidesABZR87_RS17470 and ABZR87_RS17475 are separated by 19 nucleotidesABZR87_RS17475 and ABZR87_RS17480 overlap by 1 nucleotides ABZR87_RS17465: ABZR87_RS17465 - AMP-binding protein, at 277,253 to 278,947 _RS17465 ABZR87_RS17470: ABZR87_RS17470 - CoA-acylating methylmalonate-semialdehyde dehydrogenase, at 279,020 to 280,567 _RS17470 ABZR87_RS17475: ABZR87_RS17475 - 3-hydroxyisobutyrate dehydrogenase, at 280,587 to 281,480 _RS17475 ABZR87_RS17480: ABZR87_RS17480 - enoyl-CoA hydratase, at 281,480 to 282,277 _RS17480 Position (kb) 279 280 281Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 278.064 kb on - strand, within ABZR87_RS17465at 278.070 kb on - strand, within ABZR87_RS17465at 278.070 kb on - strand, within ABZR87_RS17465at 278.116 kb on + strand, within ABZR87_RS17465at 278.183 kb on + strand, within ABZR87_RS17465at 278.184 kb on - strand, within ABZR87_RS17465at 278.513 kb on + strand, within ABZR87_RS17465at 278.513 kb on + strand, within ABZR87_RS17465at 278.516 kb on + strand, within ABZR87_RS17465at 278.517 kb on - strand, within ABZR87_RS17465at 278.517 kb on - strand, within ABZR87_RS17465at 278.517 kb on - strand, within ABZR87_RS17465at 278.564 kb on + strand, within ABZR87_RS17465at 278.565 kb on - strand, within ABZR87_RS17465at 278.565 kb on - strand, within ABZR87_RS17465at 278.798 kb on + strandat 278.799 kb on - strandat 278.925 kb on - strandat 279.474 kb on - strand, within ABZR87_RS17470at 279.773 kb on + strand, within ABZR87_RS17470at 279.773 kb on + strand, within ABZR87_RS17470at 279.773 kb on + strand, within ABZR87_RS17470at 279.774 kb on - strand, within ABZR87_RS17470at 279.774 kb on - strand, within ABZR87_RS17470at 280.200 kb on - strand, within ABZR87_RS17470at 280.205 kb on + strand, within ABZR87_RS17470at 280.205 kb on + strand, within ABZR87_RS17470at 280.205 kb on + strand, within ABZR87_RS17470at 280.205 kb on + strand, within ABZR87_RS17470at 280.206 kb on - strand, within ABZR87_RS17470at 280.206 kb on - strand, within ABZR87_RS17470at 280.252 kb on + strand, within ABZR87_RS17470at 280.406 kb on + strand, within ABZR87_RS17470at 280.481 kb on + strandat 280.547 kb on - strandat 280.683 kb on - strand, within ABZR87_RS17475at 280.795 kb on + strand, within ABZR87_RS17475at 280.795 kb on + strand, within ABZR87_RS17475at 280.795 kb on + strand, within ABZR87_RS17475at 280.795 kb on + strand, within ABZR87_RS17475at 280.795 kb on + strand, within ABZR87_RS17475at 280.795 kb on + strand, within ABZR87_RS17475at 280.795 kb on + strand, within ABZR87_RS17475at 280.796 kb on - strand, within ABZR87_RS17475at 281.239 kb on - strand, within ABZR87_RS17475at 281.239 kb on - strand, within ABZR87_RS17475at 281.239 kb on - strand, within ABZR87_RS17475at 281.286 kb on + strand, within ABZR87_RS17475at 281.370 kb on + strand, within ABZR87_RS17475at 281.370 kb on + strand, within ABZR87_RS17475at 281.458 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM
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278,064 - ABZR87_RS17465 0.48 +1.1
278,070 - ABZR87_RS17465 0.48 +0.7
278,070 - ABZR87_RS17465 0.48 +0.5
278,116 + ABZR87_RS17465 0.51 -1.1
278,183 + ABZR87_RS17465 0.55 +0.5
278,184 - ABZR87_RS17465 0.55 +0.5
278,513 + ABZR87_RS17465 0.74 -1.5
278,513 + ABZR87_RS17465 0.74 +0.8
278,516 + ABZR87_RS17465 0.75 -0.9
278,517 - ABZR87_RS17465 0.75 +1.7
278,517 - ABZR87_RS17465 0.75 +0.1
278,517 - ABZR87_RS17465 0.75 +0.4
278,564 + ABZR87_RS17465 0.77 +3.7
278,565 - ABZR87_RS17465 0.77 +0.2
278,565 - ABZR87_RS17465 0.77 +0.2
278,798 + +0.8
278,799 - +0.5
278,925 - -0.1
279,474 - ABZR87_RS17470 0.29 +0.5
279,773 + ABZR87_RS17470 0.49 +0.2
279,773 + ABZR87_RS17470 0.49 +1.5
279,773 + ABZR87_RS17470 0.49 +0.1
279,774 - ABZR87_RS17470 0.49 +0.3
279,774 - ABZR87_RS17470 0.49 +2.3
280,200 - ABZR87_RS17470 0.76 +1.3
280,205 + ABZR87_RS17470 0.77 +1.8
280,205 + ABZR87_RS17470 0.77 -0.7
280,205 + ABZR87_RS17470 0.77 +2.3
280,205 + ABZR87_RS17470 0.77 -4.6
280,206 - ABZR87_RS17470 0.77 +1.5
280,206 - ABZR87_RS17470 0.77 +0.7
280,252 + ABZR87_RS17470 0.80 -1.9
280,406 + ABZR87_RS17470 0.90 +1.1
280,481 + -0.1
280,547 - +1.1
280,683 - ABZR87_RS17475 0.11 +1.5
280,795 + ABZR87_RS17475 0.23 +1.6
280,795 + ABZR87_RS17475 0.23 +0.7
280,795 + ABZR87_RS17475 0.23 -2.1
280,795 + ABZR87_RS17475 0.23 +0.0
280,795 + ABZR87_RS17475 0.23 -0.2
280,795 + ABZR87_RS17475 0.23 +0.7
280,795 + ABZR87_RS17475 0.23 -1.9
280,796 - ABZR87_RS17475 0.23 -0.2
281,239 - ABZR87_RS17475 0.73 -0.0
281,239 - ABZR87_RS17475 0.73 +0.2
281,239 - ABZR87_RS17475 0.73 +1.2
281,286 + ABZR87_RS17475 0.78 -0.5
281,370 + ABZR87_RS17475 0.88 +0.9
281,370 + ABZR87_RS17475 0.88 -1.5
281,458 - +0.3

Or see this region's nucleotide sequence