Strain Fitness in Ralstonia sp. UNC404CL21Col around ABZR87_RS09995

Experiment: nitrogen source nitrite 0.5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABZR87_RS09980 and ABZR87_RS09985 are separated by 71 nucleotidesABZR87_RS09985 and ABZR87_RS09990 are separated by 26 nucleotidesABZR87_RS09990 and ABZR87_RS09995 are separated by 10 nucleotidesABZR87_RS09995 and ABZR87_RS10000 are separated by 34 nucleotidesABZR87_RS10000 and ABZR87_RS10005 are separated by 69 nucleotides ABZR87_RS09980: ABZR87_RS09980 - IscS subfamily cysteine desulfurase, at 2,121,791 to 2,123,008 _RS09980 ABZR87_RS09985: ABZR87_RS09985 - Fe-S cluster assembly scaffold IscU, at 2,123,080 to 2,123,481 _RS09985 ABZR87_RS09990: ABZR87_RS09990 - iron-sulfur cluster assembly protein IscA, at 2,123,508 to 2,123,828 _RS09990 ABZR87_RS09995: ABZR87_RS09995 - DUF3565 domain-containing protein, at 2,123,839 to 2,124,039 _RS09995 ABZR87_RS10000: ABZR87_RS10000 - Fe-S protein assembly co-chaperone HscB, at 2,124,074 to 2,124,613 _RS10000 ABZR87_RS10005: ABZR87_RS10005 - Fe-S protein assembly chaperone HscA, at 2,124,683 to 2,126,548 _RS10005 Position (kb) 2123 2124 2125Strain fitness (log2 ratio) -1 0 1 2at 2123.036 kb on + strandat 2124.037 kb on + strandat 2124.611 kb on + strandat 2124.611 kb on + strandat 2124.611 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM
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2,123,036 + +0.3
2,124,037 + +0.4
2,124,611 + +0.4
2,124,611 + +2.1
2,124,611 + +0.1

Or see this region's nucleotide sequence