Experiment: RCH2 defined with Nickel (II) chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5383 and Pf6N2E2_5384 are separated by 26 nucleotides Pf6N2E2_5384 and Pf6N2E2_5385 overlap by 4 nucleotides
Pf6N2E2_5383 - Poly(beta-D-mannuronate) C5 epimerase precursor (EC 5.1.3.-), at 6,185,829 to 6,187,418
_5383
Pf6N2E2_5384 - outer membrane protein AlgE, at 6,187,445 to 6,188,944
_5384
Pf6N2E2_5385 - Alginate biosynthesis protein AlgK precursor, at 6,188,941 to 6,190,341
_5385
Position (kb)
6187
6188
6189 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6186.518 kb on - strand, within Pf6N2E2_5383 at 6186.518 kb on - strand, within Pf6N2E2_5383 at 6186.518 kb on - strand, within Pf6N2E2_5383 at 6186.518 kb on - strand, within Pf6N2E2_5383 at 6186.553 kb on + strand, within Pf6N2E2_5383 at 6186.553 kb on + strand, within Pf6N2E2_5383 at 6186.554 kb on - strand, within Pf6N2E2_5383 at 6186.559 kb on + strand at 6186.559 kb on + strand, within Pf6N2E2_5383 at 6186.560 kb on - strand, within Pf6N2E2_5383 at 6186.649 kb on + strand, within Pf6N2E2_5383 at 6186.650 kb on - strand, within Pf6N2E2_5383 at 6186.659 kb on + strand, within Pf6N2E2_5383 at 6186.659 kb on + strand, within Pf6N2E2_5383 at 6186.660 kb on - strand, within Pf6N2E2_5383 at 6186.660 kb on - strand, within Pf6N2E2_5383 at 6186.677 kb on + strand, within Pf6N2E2_5383 at 6186.678 kb on - strand, within Pf6N2E2_5383 at 6186.678 kb on - strand, within Pf6N2E2_5383 at 6186.678 kb on - strand, within Pf6N2E2_5383 at 6186.678 kb on - strand, within Pf6N2E2_5383 at 6186.678 kb on - strand, within Pf6N2E2_5383 at 6186.721 kb on + strand, within Pf6N2E2_5383 at 6186.721 kb on + strand, within Pf6N2E2_5383 at 6186.721 kb on + strand, within Pf6N2E2_5383 at 6186.721 kb on + strand, within Pf6N2E2_5383 at 6186.721 kb on + strand, within Pf6N2E2_5383 at 6186.722 kb on - strand, within Pf6N2E2_5383 at 6186.722 kb on - strand, within Pf6N2E2_5383 at 6186.955 kb on + strand, within Pf6N2E2_5383 at 6186.955 kb on + strand, within Pf6N2E2_5383 at 6186.982 kb on + strand, within Pf6N2E2_5383 at 6186.982 kb on + strand, within Pf6N2E2_5383 at 6186.982 kb on + strand, within Pf6N2E2_5383 at 6186.983 kb on - strand, within Pf6N2E2_5383 at 6187.021 kb on + strand, within Pf6N2E2_5383 at 6187.021 kb on + strand, within Pf6N2E2_5383 at 6187.022 kb on - strand, within Pf6N2E2_5383 at 6187.195 kb on + strand, within Pf6N2E2_5383 at 6187.196 kb on - strand, within Pf6N2E2_5383 at 6187.264 kb on + strand at 6187.264 kb on + strand at 6187.264 kb on + strand at 6187.264 kb on + strand at 6187.264 kb on + strand at 6187.264 kb on + strand at 6187.265 kb on - strand at 6187.265 kb on - strand at 6187.265 kb on - strand at 6187.265 kb on - strand at 6187.409 kb on - strand at 6187.409 kb on - strand at 6187.485 kb on + strand at 6187.486 kb on - strand at 6187.486 kb on - strand at 6187.506 kb on + strand at 6187.609 kb on - strand, within Pf6N2E2_5384 at 6187.609 kb on - strand, within Pf6N2E2_5384 at 6187.779 kb on + strand, within Pf6N2E2_5384 at 6187.779 kb on + strand, within Pf6N2E2_5384 at 6187.779 kb on + strand, within Pf6N2E2_5384 at 6187.779 kb on + strand, within Pf6N2E2_5384 at 6187.779 kb on + strand, within Pf6N2E2_5384 at 6187.779 kb on + strand, within Pf6N2E2_5384 at 6187.780 kb on - strand, within Pf6N2E2_5384 at 6187.941 kb on + strand, within Pf6N2E2_5384 at 6187.962 kb on + strand, within Pf6N2E2_5384 at 6188.130 kb on + strand, within Pf6N2E2_5384 at 6188.131 kb on - strand, within Pf6N2E2_5384 at 6188.160 kb on + strand, within Pf6N2E2_5384 at 6188.160 kb on + strand, within Pf6N2E2_5384 at 6188.160 kb on + strand, within Pf6N2E2_5384 at 6188.160 kb on + strand, within Pf6N2E2_5384 at 6188.251 kb on - strand, within Pf6N2E2_5384 at 6188.698 kb on - strand, within Pf6N2E2_5384 at 6188.730 kb on + strand, within Pf6N2E2_5384 at 6188.731 kb on - strand, within Pf6N2E2_5384 at 6188.731 kb on - strand, within Pf6N2E2_5384 at 6188.944 kb on + strand at 6188.971 kb on + strand at 6189.031 kb on + strand at 6189.031 kb on + strand at 6189.031 kb on + strand at 6189.068 kb on - strand at 6189.143 kb on + strand, within Pf6N2E2_5385 at 6189.143 kb on + strand, within Pf6N2E2_5385 at 6189.144 kb on - strand, within Pf6N2E2_5385 at 6189.144 kb on - strand, within Pf6N2E2_5385 at 6189.144 kb on - strand, within Pf6N2E2_5385 at 6189.144 kb on - strand, within Pf6N2E2_5385 at 6189.144 kb on - strand, within Pf6N2E2_5385 at 6189.144 kb on - strand, within Pf6N2E2_5385 at 6189.144 kb on - strand, within Pf6N2E2_5385 at 6189.144 kb on - strand, within Pf6N2E2_5385 at 6189.266 kb on + strand, within Pf6N2E2_5385 at 6189.293 kb on + strand, within Pf6N2E2_5385 at 6189.293 kb on + strand, within Pf6N2E2_5385 at 6189.293 kb on + strand, within Pf6N2E2_5385 at 6189.293 kb on + strand, within Pf6N2E2_5385 at 6189.294 kb on - strand, within Pf6N2E2_5385 at 6189.308 kb on + strand, within Pf6N2E2_5385 at 6189.309 kb on - strand, within Pf6N2E2_5385 at 6189.309 kb on - strand, within Pf6N2E2_5385 at 6189.311 kb on + strand, within Pf6N2E2_5385 at 6189.395 kb on + strand, within Pf6N2E2_5385 at 6189.396 kb on - strand, within Pf6N2E2_5385 at 6189.396 kb on - strand, within Pf6N2E2_5385 at 6189.396 kb on - strand, within Pf6N2E2_5385 at 6189.396 kb on - strand, within Pf6N2E2_5385 at 6189.398 kb on + strand, within Pf6N2E2_5385 at 6189.399 kb on - strand, within Pf6N2E2_5385 at 6189.458 kb on + strand, within Pf6N2E2_5385 at 6189.458 kb on + strand, within Pf6N2E2_5385 at 6189.459 kb on - strand, within Pf6N2E2_5385 at 6189.501 kb on - strand, within Pf6N2E2_5385 at 6189.719 kb on + strand, within Pf6N2E2_5385 at 6189.720 kb on - strand, within Pf6N2E2_5385 at 6189.740 kb on + strand, within Pf6N2E2_5385 at 6189.740 kb on + strand, within Pf6N2E2_5385 at 6189.740 kb on + strand, within Pf6N2E2_5385 at 6189.741 kb on - strand, within Pf6N2E2_5385 at 6189.806 kb on + strand, within Pf6N2E2_5385 at 6189.806 kb on + strand, within Pf6N2E2_5385 at 6189.807 kb on - strand, within Pf6N2E2_5385 at 6189.807 kb on - strand, within Pf6N2E2_5385 at 6189.807 kb on - strand, within Pf6N2E2_5385 at 6189.807 kb on - strand, within Pf6N2E2_5385 at 6189.929 kb on + strand, within Pf6N2E2_5385 at 6189.929 kb on + strand, within Pf6N2E2_5385 at 6189.929 kb on + strand, within Pf6N2E2_5385 at 6189.930 kb on - strand, within Pf6N2E2_5385 at 6189.930 kb on - strand, within Pf6N2E2_5385
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Nickel (II) chloride 0.04 mM remove 6,186,518 - Pf6N2E2_5383 0.43 +0.5 6,186,518 - Pf6N2E2_5383 0.43 -2.9 6,186,518 - Pf6N2E2_5383 0.43 +1.2 6,186,518 - Pf6N2E2_5383 0.43 +1.1 6,186,553 + Pf6N2E2_5383 0.46 +0.9 6,186,553 + Pf6N2E2_5383 0.46 -0.5 6,186,554 - Pf6N2E2_5383 0.46 -0.1 6,186,559 + -0.5 6,186,559 + Pf6N2E2_5383 0.46 +1.6 6,186,560 - Pf6N2E2_5383 0.46 -1.0 6,186,649 + Pf6N2E2_5383 0.52 +1.1 6,186,650 - Pf6N2E2_5383 0.52 -1.2 6,186,659 + Pf6N2E2_5383 0.52 -0.3 6,186,659 + Pf6N2E2_5383 0.52 +0.5 6,186,660 - Pf6N2E2_5383 0.52 +0.5 6,186,660 - Pf6N2E2_5383 0.52 +1.1 6,186,677 + Pf6N2E2_5383 0.53 +0.5 6,186,678 - Pf6N2E2_5383 0.53 +0.5 6,186,678 - Pf6N2E2_5383 0.53 +0.5 6,186,678 - Pf6N2E2_5383 0.53 -1.5 6,186,678 - Pf6N2E2_5383 0.53 -1.6 6,186,678 - Pf6N2E2_5383 0.53 +1.4 6,186,721 + Pf6N2E2_5383 0.56 -1.0 6,186,721 + Pf6N2E2_5383 0.56 +0.3 6,186,721 + Pf6N2E2_5383 0.56 +0.2 6,186,721 + Pf6N2E2_5383 0.56 +0.1 6,186,721 + Pf6N2E2_5383 0.56 +0.6 6,186,722 - Pf6N2E2_5383 0.56 -1.7 6,186,722 - Pf6N2E2_5383 0.56 +1.1 6,186,955 + Pf6N2E2_5383 0.71 +0.7 6,186,955 + Pf6N2E2_5383 0.71 +0.1 6,186,982 + Pf6N2E2_5383 0.73 -0.1 6,186,982 + Pf6N2E2_5383 0.73 -1.3 6,186,982 + Pf6N2E2_5383 0.73 -1.7 6,186,983 - Pf6N2E2_5383 0.73 +0.9 6,187,021 + Pf6N2E2_5383 0.75 -0.3 6,187,021 + Pf6N2E2_5383 0.75 -0.4 6,187,022 - Pf6N2E2_5383 0.75 -0.8 6,187,195 + Pf6N2E2_5383 0.86 +2.1 6,187,196 - Pf6N2E2_5383 0.86 +0.5 6,187,264 + -0.5 6,187,264 + +0.0 6,187,264 + -1.8 6,187,264 + +0.2 6,187,264 + +0.1 6,187,264 + -0.2 6,187,265 - +1.1 6,187,265 - -0.4 6,187,265 - -0.2 6,187,265 - -1.2 6,187,409 - +0.7 6,187,409 - +0.4 6,187,485 + +0.1 6,187,486 - -0.9 6,187,486 - +1.9 6,187,506 + -0.5 6,187,609 - Pf6N2E2_5384 0.11 -0.1 6,187,609 - Pf6N2E2_5384 0.11 -0.0 6,187,779 + Pf6N2E2_5384 0.22 -1.0 6,187,779 + Pf6N2E2_5384 0.22 -1.6 6,187,779 + Pf6N2E2_5384 0.22 -0.2 6,187,779 + Pf6N2E2_5384 0.22 +0.8 6,187,779 + Pf6N2E2_5384 0.22 +0.8 6,187,779 + Pf6N2E2_5384 0.22 -0.1 6,187,780 - Pf6N2E2_5384 0.22 +2.8 6,187,941 + Pf6N2E2_5384 0.33 -1.8 6,187,962 + Pf6N2E2_5384 0.34 +0.1 6,188,130 + Pf6N2E2_5384 0.46 +0.2 6,188,131 - Pf6N2E2_5384 0.46 -1.2 6,188,160 + Pf6N2E2_5384 0.48 -1.7 6,188,160 + Pf6N2E2_5384 0.48 +0.1 6,188,160 + Pf6N2E2_5384 0.48 +0.6 6,188,160 + Pf6N2E2_5384 0.48 -1.5 6,188,251 - Pf6N2E2_5384 0.54 -0.4 6,188,698 - Pf6N2E2_5384 0.84 +1.6 6,188,730 + Pf6N2E2_5384 0.86 -0.0 6,188,731 - Pf6N2E2_5384 0.86 +0.1 6,188,731 - Pf6N2E2_5384 0.86 +0.1 6,188,944 + +0.7 6,188,971 + -0.5 6,189,031 + -0.4 6,189,031 + -1.6 6,189,031 + +0.2 6,189,068 - -1.5 6,189,143 + Pf6N2E2_5385 0.14 +0.8 6,189,143 + Pf6N2E2_5385 0.14 +0.4 6,189,144 - Pf6N2E2_5385 0.14 -0.0 6,189,144 - Pf6N2E2_5385 0.14 +1.1 6,189,144 - Pf6N2E2_5385 0.14 -0.4 6,189,144 - Pf6N2E2_5385 0.14 +1.1 6,189,144 - Pf6N2E2_5385 0.14 -1.2 6,189,144 - Pf6N2E2_5385 0.14 -0.1 6,189,144 - Pf6N2E2_5385 0.14 -0.9 6,189,144 - Pf6N2E2_5385 0.14 +0.3 6,189,266 + Pf6N2E2_5385 0.23 +1.1 6,189,293 + Pf6N2E2_5385 0.25 -1.1 6,189,293 + Pf6N2E2_5385 0.25 -1.6 6,189,293 + Pf6N2E2_5385 0.25 -0.2 6,189,293 + Pf6N2E2_5385 0.25 +0.9 6,189,294 - Pf6N2E2_5385 0.25 -0.0 6,189,308 + Pf6N2E2_5385 0.26 -0.9 6,189,309 - Pf6N2E2_5385 0.26 -3.6 6,189,309 - Pf6N2E2_5385 0.26 +0.3 6,189,311 + Pf6N2E2_5385 0.26 -0.9 6,189,395 + Pf6N2E2_5385 0.32 +0.2 6,189,396 - Pf6N2E2_5385 0.32 +0.4 6,189,396 - Pf6N2E2_5385 0.32 +1.1 6,189,396 - Pf6N2E2_5385 0.32 -1.2 6,189,396 - Pf6N2E2_5385 0.32 -0.4 6,189,398 + Pf6N2E2_5385 0.33 +0.2 6,189,399 - Pf6N2E2_5385 0.33 -0.4 6,189,458 + Pf6N2E2_5385 0.37 -0.9 6,189,458 + Pf6N2E2_5385 0.37 +0.4 6,189,459 - Pf6N2E2_5385 0.37 -0.4 6,189,501 - Pf6N2E2_5385 0.40 +0.8 6,189,719 + Pf6N2E2_5385 0.56 -0.2 6,189,720 - Pf6N2E2_5385 0.56 -0.1 6,189,740 + Pf6N2E2_5385 0.57 +0.9 6,189,740 + Pf6N2E2_5385 0.57 -1.9 6,189,740 + Pf6N2E2_5385 0.57 -1.5 6,189,741 - Pf6N2E2_5385 0.57 +0.0 6,189,806 + Pf6N2E2_5385 0.62 -0.2 6,189,806 + Pf6N2E2_5385 0.62 -0.0 6,189,807 - Pf6N2E2_5385 0.62 +0.8 6,189,807 - Pf6N2E2_5385 0.62 +0.2 6,189,807 - Pf6N2E2_5385 0.62 +1.0 6,189,807 - Pf6N2E2_5385 0.62 +0.5 6,189,929 + Pf6N2E2_5385 0.71 +0.6 6,189,929 + Pf6N2E2_5385 0.71 +0.0 6,189,929 + Pf6N2E2_5385 0.71 -1.5 6,189,930 - Pf6N2E2_5385 0.71 -1.2 6,189,930 - Pf6N2E2_5385 0.71 -0.4
Or see this region's nucleotide sequence