Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_203

Experiment: RCH2 defined with Nickel (II) chloride 0.04 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_202 and Pf6N2E2_203 overlap by 11 nucleotidesPf6N2E2_203 and Pf6N2E2_204 are separated by 2 nucleotidesPf6N2E2_204 and Pf6N2E2_205 overlap by 4 nucleotides Pf6N2E2_202 - 4Fe-4S ferredoxin, iron-sulfur binding, at 253,064 to 254,650 _202 Pf6N2E2_203 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 254,640 to 255,920 _203 Pf6N2E2_204 - hypothetical protein, at 255,923 to 256,330 _204 Pf6N2E2_205 - hypothetical protein, at 256,327 to 257,073 _205 Position (kb) 254 255 256Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 253.941 kb on + strand, within Pf6N2E2_202at 253.941 kb on + strand, within Pf6N2E2_202at 253.942 kb on - strand, within Pf6N2E2_202at 253.942 kb on - strand, within Pf6N2E2_202at 253.942 kb on - strand, within Pf6N2E2_202at 253.942 kb on - strand, within Pf6N2E2_202at 253.942 kb on - strand, within Pf6N2E2_202at 253.942 kb on - strand, within Pf6N2E2_202at 253.942 kb on - strand, within Pf6N2E2_202at 253.942 kb on - strand, within Pf6N2E2_202at 253.942 kb on - strand, within Pf6N2E2_202at 253.942 kb on - strand, within Pf6N2E2_202at 254.157 kb on + strand, within Pf6N2E2_202at 254.157 kb on + strand, within Pf6N2E2_202at 254.157 kb on + strand, within Pf6N2E2_202at 254.157 kb on + strand, within Pf6N2E2_202at 254.158 kb on - strand, within Pf6N2E2_202at 254.158 kb on - strand, within Pf6N2E2_202at 254.158 kb on - strand, within Pf6N2E2_202at 254.218 kb on - strand, within Pf6N2E2_202at 254.218 kb on - strand, within Pf6N2E2_202at 254.239 kb on - strand, within Pf6N2E2_202at 254.826 kb on + strand, within Pf6N2E2_203at 254.960 kb on + strand, within Pf6N2E2_203at 255.029 kb on - strand, within Pf6N2E2_203at 255.232 kb on - strand, within Pf6N2E2_203at 255.384 kb on + strand, within Pf6N2E2_203at 255.416 kb on + strand, within Pf6N2E2_203at 255.416 kb on + strand, within Pf6N2E2_203at 255.416 kb on + strand, within Pf6N2E2_203at 255.416 kb on + strand, within Pf6N2E2_203at 255.416 kb on + strand, within Pf6N2E2_203at 255.416 kb on + strand, within Pf6N2E2_203at 255.417 kb on - strand, within Pf6N2E2_203at 255.514 kb on + strand, within Pf6N2E2_203at 255.514 kb on + strand, within Pf6N2E2_203at 255.514 kb on + strand, within Pf6N2E2_203at 255.515 kb on - strand, within Pf6N2E2_203at 255.515 kb on - strand, within Pf6N2E2_203at 255.515 kb on - strand, within Pf6N2E2_203at 255.803 kb on + strandat 255.803 kb on + strandat 255.803 kb on + strandat 255.803 kb on + strandat 255.803 kb on + strandat 255.803 kb on + strandat 255.803 kb on + strandat 255.805 kb on + strandat 255.805 kb on + strandat 255.805 kb on + strandat 255.805 kb on + strandat 255.806 kb on - strandat 255.806 kb on - strandat 255.806 kb on - strandat 255.890 kb on - strandat 255.912 kb on + strandat 256.062 kb on + strand, within Pf6N2E2_204at 256.062 kb on + strand, within Pf6N2E2_204at 256.062 kb on + strand, within Pf6N2E2_204at 256.321 kb on + strandat 256.478 kb on + strand, within Pf6N2E2_205at 256.478 kb on + strand, within Pf6N2E2_205at 256.479 kb on - strand, within Pf6N2E2_205at 256.562 kb on + strand, within Pf6N2E2_205at 256.562 kb on + strand, within Pf6N2E2_205at 256.563 kb on - strand, within Pf6N2E2_205at 256.563 kb on - strand, within Pf6N2E2_205at 256.563 kb on - strand, within Pf6N2E2_205at 256.659 kb on + strand, within Pf6N2E2_205at 256.661 kb on + strand, within Pf6N2E2_205at 256.661 kb on + strand, within Pf6N2E2_205at 256.882 kb on + strand, within Pf6N2E2_205at 256.883 kb on - strand, within Pf6N2E2_205at 256.892 kb on + strand, within Pf6N2E2_205

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2 defined with Nickel (II) chloride 0.04 mM
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253,941 + Pf6N2E2_202 0.55 -1.9
253,941 + Pf6N2E2_202 0.55 +0.3
253,942 - Pf6N2E2_202 0.55 +1.3
253,942 - Pf6N2E2_202 0.55 -1.8
253,942 - Pf6N2E2_202 0.55 -0.8
253,942 - Pf6N2E2_202 0.55 -0.2
253,942 - Pf6N2E2_202 0.55 +0.9
253,942 - Pf6N2E2_202 0.55 +0.7
253,942 - Pf6N2E2_202 0.55 +0.3
253,942 - Pf6N2E2_202 0.55 -0.8
253,942 - Pf6N2E2_202 0.55 +0.2
253,942 - Pf6N2E2_202 0.55 +0.5
254,157 + Pf6N2E2_202 0.69 +0.9
254,157 + Pf6N2E2_202 0.69 +0.3
254,157 + Pf6N2E2_202 0.69 -0.2
254,157 + Pf6N2E2_202 0.69 +2.0
254,158 - Pf6N2E2_202 0.69 -2.5
254,158 - Pf6N2E2_202 0.69 -0.8
254,158 - Pf6N2E2_202 0.69 +0.0
254,218 - Pf6N2E2_202 0.73 +0.1
254,218 - Pf6N2E2_202 0.73 -0.8
254,239 - Pf6N2E2_202 0.74 +0.5
254,826 + Pf6N2E2_203 0.15 -0.4
254,960 + Pf6N2E2_203 0.25 -0.2
255,029 - Pf6N2E2_203 0.30 -0.2
255,232 - Pf6N2E2_203 0.46 -3.0
255,384 + Pf6N2E2_203 0.58 -0.8
255,416 + Pf6N2E2_203 0.61 +0.0
255,416 + Pf6N2E2_203 0.61 +0.5
255,416 + Pf6N2E2_203 0.61 +1.1
255,416 + Pf6N2E2_203 0.61 +0.4
255,416 + Pf6N2E2_203 0.61 -1.5
255,416 + Pf6N2E2_203 0.61 -1.0
255,417 - Pf6N2E2_203 0.61 -0.2
255,514 + Pf6N2E2_203 0.68 -0.4
255,514 + Pf6N2E2_203 0.68 +0.3
255,514 + Pf6N2E2_203 0.68 -0.7
255,515 - Pf6N2E2_203 0.68 -0.2
255,515 - Pf6N2E2_203 0.68 -1.0
255,515 - Pf6N2E2_203 0.68 -0.5
255,803 + -0.8
255,803 + +0.8
255,803 + +0.1
255,803 + -0.8
255,803 + +0.5
255,803 + +1.0
255,803 + -0.4
255,805 + +2.8
255,805 + -0.2
255,805 + +0.0
255,805 + +1.8
255,806 - +1.4
255,806 - +0.1
255,806 - -0.2
255,890 - -0.9
255,912 + -0.2
256,062 + Pf6N2E2_204 0.34 +0.1
256,062 + Pf6N2E2_204 0.34 +0.5
256,062 + Pf6N2E2_204 0.34 -0.8
256,321 + +1.1
256,478 + Pf6N2E2_205 0.20 -0.3
256,478 + Pf6N2E2_205 0.20 +1.4
256,479 - Pf6N2E2_205 0.20 +1.5
256,562 + Pf6N2E2_205 0.31 -2.2
256,562 + Pf6N2E2_205 0.31 -0.2
256,563 - Pf6N2E2_205 0.32 -0.9
256,563 - Pf6N2E2_205 0.32 +0.4
256,563 - Pf6N2E2_205 0.32 -1.8
256,659 + Pf6N2E2_205 0.44 -1.3
256,661 + Pf6N2E2_205 0.45 -0.6
256,661 + Pf6N2E2_205 0.45 +0.3
256,882 + Pf6N2E2_205 0.74 -0.9
256,883 - Pf6N2E2_205 0.74 -1.5
256,892 + Pf6N2E2_205 0.76 +0.8

Or see this region's nucleotide sequence