Experiment: RCH2 defined with Nickel (II) chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1003 and Pf6N2E2_1004 are separated by 88 nucleotides Pf6N2E2_1004 and Pf6N2E2_1005 are separated by 85 nucleotides Pf6N2E2_1005 and Pf6N2E2_1006 are separated by 486 nucleotides
Pf6N2E2_1003 - Fucose permease, at 1,172,313 to 1,173,695
_1003
Pf6N2E2_1004 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 1,173,784 to 1,174,542
_1004
Pf6N2E2_1005 - Ribose ABC transport system, periplasmic ribose-binding protein RbsB (TC 3.A.1.2.1), at 1,174,628 to 1,175,581
_1005
Pf6N2E2_1006 - Ribose ABC transport system, periplasmic ribose-binding protein RbsB (TC 3.A.1.2.1), at 1,176,068 to 1,176,601
_1006
Position (kb)
1174
1175
1176 Strain fitness (log2 ratio)
-2
-1
0
1 at 1173.684 kb on - strand at 1173.722 kb on + strand at 1173.722 kb on + strand at 1173.778 kb on + strand at 1173.779 kb on - strand at 1173.816 kb on + strand at 1173.827 kb on + strand at 1173.827 kb on + strand at 1173.827 kb on + strand at 1173.828 kb on - strand at 1173.831 kb on + strand at 1173.831 kb on + strand at 1173.832 kb on - strand at 1173.832 kb on - strand at 1173.832 kb on - strand at 1173.832 kb on - strand at 1173.832 kb on - strand at 1173.946 kb on + strand, within Pf6N2E2_1004 at 1173.947 kb on - strand, within Pf6N2E2_1004 at 1173.947 kb on - strand, within Pf6N2E2_1004 at 1173.947 kb on - strand, within Pf6N2E2_1004 at 1173.958 kb on - strand, within Pf6N2E2_1004 at 1174.023 kb on - strand, within Pf6N2E2_1004 at 1174.049 kb on + strand, within Pf6N2E2_1004 at 1174.049 kb on + strand, within Pf6N2E2_1004 at 1174.074 kb on - strand, within Pf6N2E2_1004 at 1174.081 kb on + strand, within Pf6N2E2_1004 at 1174.132 kb on - strand, within Pf6N2E2_1004 at 1174.153 kb on + strand, within Pf6N2E2_1004 at 1174.313 kb on - strand, within Pf6N2E2_1004 at 1174.422 kb on + strand, within Pf6N2E2_1004 at 1174.423 kb on - strand, within Pf6N2E2_1004 at 1174.423 kb on - strand, within Pf6N2E2_1004 at 1174.423 kb on - strand, within Pf6N2E2_1004 at 1174.466 kb on + strand, within Pf6N2E2_1004 at 1174.466 kb on + strand, within Pf6N2E2_1004 at 1174.693 kb on - strand at 1174.759 kb on + strand, within Pf6N2E2_1005 at 1174.759 kb on + strand, within Pf6N2E2_1005 at 1174.763 kb on + strand, within Pf6N2E2_1005 at 1174.763 kb on + strand, within Pf6N2E2_1005 at 1174.763 kb on + strand, within Pf6N2E2_1005 at 1174.763 kb on + strand, within Pf6N2E2_1005 at 1174.764 kb on - strand, within Pf6N2E2_1005 at 1174.764 kb on - strand, within Pf6N2E2_1005 at 1174.764 kb on - strand, within Pf6N2E2_1005 at 1174.806 kb on + strand, within Pf6N2E2_1005 at 1174.806 kb on + strand, within Pf6N2E2_1005 at 1174.806 kb on + strand, within Pf6N2E2_1005 at 1174.807 kb on - strand, within Pf6N2E2_1005 at 1174.947 kb on + strand, within Pf6N2E2_1005 at 1174.947 kb on + strand, within Pf6N2E2_1005 at 1174.947 kb on + strand, within Pf6N2E2_1005 at 1174.948 kb on - strand, within Pf6N2E2_1005 at 1175.013 kb on - strand, within Pf6N2E2_1005 at 1175.048 kb on - strand, within Pf6N2E2_1005 at 1175.048 kb on - strand, within Pf6N2E2_1005 at 1175.285 kb on - strand, within Pf6N2E2_1005 at 1175.564 kb on + strand at 1175.565 kb on - strand at 1175.565 kb on - strand at 1175.593 kb on + strand at 1175.621 kb on + strand at 1175.621 kb on + strand at 1175.621 kb on + strand at 1175.621 kb on + strand at 1175.621 kb on + strand at 1175.621 kb on + strand at 1175.621 kb on + strand at 1175.621 kb on + strand at 1175.622 kb on - strand at 1175.625 kb on + strand at 1175.626 kb on - strand at 1175.676 kb on + strand at 1175.813 kb on - strand at 1175.916 kb on + strand at 1175.964 kb on + strand at 1175.964 kb on + strand at 1175.964 kb on + strand at 1175.965 kb on - strand at 1175.965 kb on - strand at 1176.538 kb on + strand, within Pf6N2E2_1006 at 1176.538 kb on + strand, within Pf6N2E2_1006 at 1176.538 kb on + strand, within Pf6N2E2_1006 at 1176.538 kb on + strand, within Pf6N2E2_1006 at 1176.538 kb on + strand, within Pf6N2E2_1006 at 1176.539 kb on - strand, within Pf6N2E2_1006
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Nickel (II) chloride 0.04 mM remove 1,173,684 - +0.5 1,173,722 + -1.0 1,173,722 + -0.8 1,173,778 + +0.5 1,173,779 - -0.3 1,173,816 + +0.5 1,173,827 + -0.5 1,173,827 + -1.6 1,173,827 + -0.5 1,173,828 - +1.0 1,173,831 + -1.5 1,173,831 + -1.3 1,173,832 - -0.1 1,173,832 - -0.5 1,173,832 - -0.3 1,173,832 - +1.7 1,173,832 - -1.8 1,173,946 + Pf6N2E2_1004 0.21 +0.1 1,173,947 - Pf6N2E2_1004 0.21 +1.0 1,173,947 - Pf6N2E2_1004 0.21 -1.5 1,173,947 - Pf6N2E2_1004 0.21 +1.1 1,173,958 - Pf6N2E2_1004 0.23 +0.5 1,174,023 - Pf6N2E2_1004 0.31 -0.4 1,174,049 + Pf6N2E2_1004 0.35 -0.1 1,174,049 + Pf6N2E2_1004 0.35 -0.5 1,174,074 - Pf6N2E2_1004 0.38 -0.5 1,174,081 + Pf6N2E2_1004 0.39 +0.5 1,174,132 - Pf6N2E2_1004 0.46 +0.5 1,174,153 + Pf6N2E2_1004 0.49 +1.1 1,174,313 - Pf6N2E2_1004 0.70 +0.5 1,174,422 + Pf6N2E2_1004 0.84 -2.0 1,174,423 - Pf6N2E2_1004 0.84 +0.7 1,174,423 - Pf6N2E2_1004 0.84 -0.1 1,174,423 - Pf6N2E2_1004 0.84 +0.2 1,174,466 + Pf6N2E2_1004 0.90 -0.4 1,174,466 + Pf6N2E2_1004 0.90 -0.5 1,174,693 - +0.5 1,174,759 + Pf6N2E2_1005 0.14 +0.5 1,174,759 + Pf6N2E2_1005 0.14 -1.4 1,174,763 + Pf6N2E2_1005 0.14 -0.5 1,174,763 + Pf6N2E2_1005 0.14 -2.0 1,174,763 + Pf6N2E2_1005 0.14 -1.5 1,174,763 + Pf6N2E2_1005 0.14 +0.5 1,174,764 - Pf6N2E2_1005 0.14 +0.2 1,174,764 - Pf6N2E2_1005 0.14 -0.5 1,174,764 - Pf6N2E2_1005 0.14 +0.5 1,174,806 + Pf6N2E2_1005 0.19 +0.5 1,174,806 + Pf6N2E2_1005 0.19 +0.5 1,174,806 + Pf6N2E2_1005 0.19 +0.5 1,174,807 - Pf6N2E2_1005 0.19 -1.0 1,174,947 + Pf6N2E2_1005 0.33 -2.3 1,174,947 + Pf6N2E2_1005 0.33 -0.0 1,174,947 + Pf6N2E2_1005 0.33 +1.3 1,174,948 - Pf6N2E2_1005 0.34 -0.5 1,175,013 - Pf6N2E2_1005 0.40 +0.5 1,175,048 - Pf6N2E2_1005 0.44 -0.5 1,175,048 - Pf6N2E2_1005 0.44 +0.1 1,175,285 - Pf6N2E2_1005 0.69 +0.5 1,175,564 + -0.3 1,175,565 - +0.5 1,175,565 - -1.0 1,175,593 + +0.2 1,175,621 + +0.5 1,175,621 + +0.2 1,175,621 + +0.9 1,175,621 + -1.0 1,175,621 + -0.5 1,175,621 + -0.7 1,175,621 + -0.8 1,175,621 + +1.1 1,175,622 - -1.0 1,175,625 + +0.6 1,175,626 - +1.1 1,175,676 + -1.5 1,175,813 - -1.8 1,175,916 + +0.8 1,175,964 + -0.7 1,175,964 + +1.1 1,175,964 + -0.0 1,175,965 - -1.5 1,175,965 - -1.0 1,176,538 + Pf6N2E2_1006 0.88 +1.5 1,176,538 + Pf6N2E2_1006 0.88 +0.8 1,176,538 + Pf6N2E2_1006 0.88 -0.5 1,176,538 + Pf6N2E2_1006 0.88 -0.7 1,176,538 + Pf6N2E2_1006 0.88 +1.2 1,176,539 - Pf6N2E2_1006 0.88 +1.5
Or see this region's nucleotide sequence