Experiment: nitrogen source nitrite 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS10345 and ABZR87_RS10350 overlap by 8 nucleotides ABZR87_RS10350 and ABZR87_RS10355 are separated by 98 nucleotides
ABZR87_RS10345: ABZR87_RS10345 - 3-isopropylmalate dehydratase large subunit, at 2,193,957 to 2,195,378
_RS10345
ABZR87_RS10350: ABZR87_RS10350 - 3-isopropylmalate dehydratase small subunit, at 2,195,371 to 2,196,033
_RS10350
ABZR87_RS10355: ABZR87_RS10355 - 2-hydroxycarboxylate transporter family protein, at 2,196,132 to 2,197,454
_RS10355
Position (kb)
2195
2196
2197 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2194.468 kb on + strand, within ABZR87_RS10345 at 2194.469 kb on - strand, within ABZR87_RS10345 at 2194.469 kb on - strand, within ABZR87_RS10345 at 2194.469 kb on - strand, within ABZR87_RS10345 at 2194.469 kb on - strand, within ABZR87_RS10345 at 2194.469 kb on - strand, within ABZR87_RS10345 at 2194.469 kb on - strand, within ABZR87_RS10345 at 2194.469 kb on - strand, within ABZR87_RS10345 at 2194.683 kb on + strand, within ABZR87_RS10345 at 2194.683 kb on + strand, within ABZR87_RS10345 at 2194.684 kb on - strand, within ABZR87_RS10345 at 2194.716 kb on - strand, within ABZR87_RS10345 at 2194.716 kb on - strand, within ABZR87_RS10345 at 2194.716 kb on - strand, within ABZR87_RS10345 at 2194.716 kb on - strand, within ABZR87_RS10345 at 2194.723 kb on + strand, within ABZR87_RS10345 at 2194.723 kb on + strand, within ABZR87_RS10345 at 2194.723 kb on + strand, within ABZR87_RS10345 at 2194.723 kb on + strand, within ABZR87_RS10345 at 2194.723 kb on + strand, within ABZR87_RS10345 at 2194.724 kb on - strand, within ABZR87_RS10345 at 2194.724 kb on - strand, within ABZR87_RS10345 at 2194.818 kb on + strand, within ABZR87_RS10345 at 2194.818 kb on + strand, within ABZR87_RS10345 at 2194.819 kb on - strand, within ABZR87_RS10345 at 2194.918 kb on - strand, within ABZR87_RS10345 at 2194.919 kb on + strand, within ABZR87_RS10345 at 2194.920 kb on - strand, within ABZR87_RS10345 at 2195.310 kb on - strand at 2195.392 kb on + strand at 2195.501 kb on - strand, within ABZR87_RS10350 at 2195.564 kb on - strand, within ABZR87_RS10350 at 2195.564 kb on - strand, within ABZR87_RS10350 at 2195.830 kb on - strand, within ABZR87_RS10350 at 2196.195 kb on + strand at 2196.226 kb on - strand at 2196.417 kb on + strand, within ABZR87_RS10355 at 2196.417 kb on + strand, within ABZR87_RS10355 at 2196.418 kb on - strand, within ABZR87_RS10355 at 2196.483 kb on + strand, within ABZR87_RS10355 at 2196.483 kb on + strand, within ABZR87_RS10355 at 2196.483 kb on + strand, within ABZR87_RS10355 at 2196.483 kb on + strand, within ABZR87_RS10355 at 2196.484 kb on - strand, within ABZR87_RS10355 at 2196.484 kb on - strand, within ABZR87_RS10355 at 2196.484 kb on - strand, within ABZR87_RS10355 at 2196.484 kb on - strand, within ABZR87_RS10355 at 2196.484 kb on - strand, within ABZR87_RS10355 at 2196.484 kb on - strand, within ABZR87_RS10355 at 2196.489 kb on + strand, within ABZR87_RS10355 at 2196.489 kb on + strand, within ABZR87_RS10355 at 2196.489 kb on + strand, within ABZR87_RS10355 at 2196.489 kb on + strand, within ABZR87_RS10355 at 2196.489 kb on + strand, within ABZR87_RS10355 at 2196.490 kb on - strand, within ABZR87_RS10355 at 2196.490 kb on - strand, within ABZR87_RS10355 at 2196.490 kb on - strand, within ABZR87_RS10355 at 2196.490 kb on - strand, within ABZR87_RS10355 at 2196.490 kb on - strand, within ABZR87_RS10355 at 2196.490 kb on - strand, within ABZR87_RS10355 at 2196.490 kb on - strand, within ABZR87_RS10355 at 2196.821 kb on + strand, within ABZR87_RS10355 at 2196.821 kb on + strand, within ABZR87_RS10355 at 2196.822 kb on - strand, within ABZR87_RS10355
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 1mM remove 2,194,468 + ABZR87_RS10345 0.36 +0.1 2,194,469 - ABZR87_RS10345 0.36 +0.6 2,194,469 - ABZR87_RS10345 0.36 +0.2 2,194,469 - ABZR87_RS10345 0.36 -0.1 2,194,469 - ABZR87_RS10345 0.36 +1.0 2,194,469 - ABZR87_RS10345 0.36 +1.2 2,194,469 - ABZR87_RS10345 0.36 -1.2 2,194,469 - ABZR87_RS10345 0.36 +0.7 2,194,683 + ABZR87_RS10345 0.51 +0.1 2,194,683 + ABZR87_RS10345 0.51 +1.4 2,194,684 - ABZR87_RS10345 0.51 -0.0 2,194,716 - ABZR87_RS10345 0.53 -0.0 2,194,716 - ABZR87_RS10345 0.53 +0.4 2,194,716 - ABZR87_RS10345 0.53 -0.1 2,194,716 - ABZR87_RS10345 0.53 +0.9 2,194,723 + ABZR87_RS10345 0.54 +0.7 2,194,723 + ABZR87_RS10345 0.54 -0.6 2,194,723 + ABZR87_RS10345 0.54 -0.5 2,194,723 + ABZR87_RS10345 0.54 +0.4 2,194,723 + ABZR87_RS10345 0.54 +0.1 2,194,724 - ABZR87_RS10345 0.54 -1.0 2,194,724 - ABZR87_RS10345 0.54 +0.7 2,194,818 + ABZR87_RS10345 0.61 -0.6 2,194,818 + ABZR87_RS10345 0.61 -1.5 2,194,819 - ABZR87_RS10345 0.61 -0.3 2,194,918 - ABZR87_RS10345 0.68 -0.4 2,194,919 + ABZR87_RS10345 0.68 +0.7 2,194,920 - ABZR87_RS10345 0.68 -3.0 2,195,310 - -0.3 2,195,392 + +1.4 2,195,501 - ABZR87_RS10350 0.20 -1.3 2,195,564 - ABZR87_RS10350 0.29 -0.2 2,195,564 - ABZR87_RS10350 0.29 -0.3 2,195,830 - ABZR87_RS10350 0.69 +4.4 2,196,195 + -0.2 2,196,226 - +0.2 2,196,417 + ABZR87_RS10355 0.22 -0.8 2,196,417 + ABZR87_RS10355 0.22 +0.2 2,196,418 - ABZR87_RS10355 0.22 +0.2 2,196,483 + ABZR87_RS10355 0.27 +0.6 2,196,483 + ABZR87_RS10355 0.27 -0.9 2,196,483 + ABZR87_RS10355 0.27 +0.4 2,196,483 + ABZR87_RS10355 0.27 +0.4 2,196,484 - ABZR87_RS10355 0.27 +0.9 2,196,484 - ABZR87_RS10355 0.27 -0.4 2,196,484 - ABZR87_RS10355 0.27 +0.4 2,196,484 - ABZR87_RS10355 0.27 -0.3 2,196,484 - ABZR87_RS10355 0.27 +0.1 2,196,484 - ABZR87_RS10355 0.27 +0.3 2,196,489 + ABZR87_RS10355 0.27 -1.4 2,196,489 + ABZR87_RS10355 0.27 +1.9 2,196,489 + ABZR87_RS10355 0.27 +0.5 2,196,489 + ABZR87_RS10355 0.27 -0.2 2,196,489 + ABZR87_RS10355 0.27 -0.5 2,196,490 - ABZR87_RS10355 0.27 -0.7 2,196,490 - ABZR87_RS10355 0.27 +1.2 2,196,490 - ABZR87_RS10355 0.27 -2.3 2,196,490 - ABZR87_RS10355 0.27 -0.0 2,196,490 - ABZR87_RS10355 0.27 -0.5 2,196,490 - ABZR87_RS10355 0.27 +0.3 2,196,490 - ABZR87_RS10355 0.27 -0.3 2,196,821 + ABZR87_RS10355 0.52 +0.5 2,196,821 + ABZR87_RS10355 0.52 -0.2 2,196,822 - ABZR87_RS10355 0.52 +0.8
Or see this region's nucleotide sequence