Experiment: nitrogen source nitrite 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS02145 and ABZR87_RS02150 overlap by 1 nucleotides ABZR87_RS02150 and ABZR87_RS02155 are separated by 13 nucleotides ABZR87_RS02155 and ABZR87_RS02160 are separated by 1 nucleotides ABZR87_RS02160 and ABZR87_RS02165 are separated by 10 nucleotides
ABZR87_RS02145: ABZR87_RS02145 - aminodeoxychorismate/anthranilate synthase component II, at 460,673 to 461,242
_RS02145
ABZR87_RS02150: ABZR87_RS02150 - lysozyme inhibitor LprI family protein, at 461,242 to 461,679
_RS02150
ABZR87_RS02155: ABZR87_RS02155 - anthranilate phosphoribosyltransferase, at 461,693 to 462,730
_RS02155
ABZR87_RS02160: ABZR87_RS02160 - indole-3-glycerol phosphate synthase TrpC, at 462,732 to 463,535
_RS02160
ABZR87_RS02165: ABZR87_RS02165 - CYTH domain-containing protein, at 463,546 to 464,205
_RS02165
Position (kb) 461
462
463 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 460.694 kb on + strand at 460.694 kb on + strand at 460.694 kb on + strand at 460.694 kb on + strand at 460.694 kb on + strand at 460.694 kb on + strand at 460.694 kb on + strand at 460.694 kb on + strand at 460.695 kb on - strand at 460.695 kb on - strand at 460.695 kb on - strand at 460.695 kb on - strand at 460.695 kb on - strand at 460.705 kb on + strand at 460.705 kb on + strand at 460.705 kb on + strand at 460.706 kb on - strand at 460.706 kb on - strand at 460.706 kb on - strand at 460.709 kb on + strand at 460.709 kb on + strand at 460.709 kb on + strand at 460.709 kb on + strand at 460.709 kb on + strand at 460.710 kb on - strand at 460.710 kb on - strand at 460.710 kb on - strand at 460.710 kb on - strand at 460.710 kb on - strand at 460.710 kb on - strand at 460.710 kb on - strand at 460.724 kb on + strand at 460.724 kb on + strand at 460.724 kb on + strand at 461.051 kb on + strand, within ABZR87_RS02145 at 461.051 kb on + strand, within ABZR87_RS02145 at 461.052 kb on - strand, within ABZR87_RS02145 at 461.101 kb on + strand, within ABZR87_RS02145 at 461.512 kb on + strand, within ABZR87_RS02150 at 461.513 kb on - strand, within ABZR87_RS02150 at 461.513 kb on - strand, within ABZR87_RS02150 at 461.513 kb on - strand, within ABZR87_RS02150 at 461.681 kb on + strand at 461.681 kb on + strand at 461.681 kb on + strand at 461.681 kb on + strand at 461.682 kb on - strand at 461.728 kb on + strand at 461.729 kb on - strand at 462.025 kb on + strand, within ABZR87_RS02155 at 462.025 kb on + strand, within ABZR87_RS02155 at 462.025 kb on + strand, within ABZR87_RS02155 at 462.025 kb on + strand, within ABZR87_RS02155 at 462.026 kb on - strand, within ABZR87_RS02155 at 462.447 kb on - strand, within ABZR87_RS02155 at 462.498 kb on + strand, within ABZR87_RS02155 at 462.498 kb on + strand, within ABZR87_RS02155 at 462.499 kb on - strand, within ABZR87_RS02155 at 462.499 kb on - strand, within ABZR87_RS02155 at 462.499 kb on - strand, within ABZR87_RS02155 at 462.499 kb on - strand, within ABZR87_RS02155 at 462.605 kb on + strand, within ABZR87_RS02155 at 462.605 kb on + strand, within ABZR87_RS02155 at 462.606 kb on - strand, within ABZR87_RS02155 at 462.606 kb on - strand, within ABZR87_RS02155 at 462.730 kb on + strand at 462.731 kb on + strand at 462.731 kb on + strand at 462.731 kb on + strand at 462.731 kb on + strand at 462.731 kb on + strand at 462.732 kb on - strand at 462.732 kb on - strand at 462.732 kb on - strand at 462.732 kb on - strand at 462.732 kb on - strand at 462.732 kb on - strand at 462.732 kb on - strand at 462.732 kb on - strand at 462.732 kb on - strand at 462.732 kb on - strand at 462.732 kb on - strand at 462.993 kb on + strand, within ABZR87_RS02160 at 462.993 kb on + strand, within ABZR87_RS02160 at 462.993 kb on + strand, within ABZR87_RS02160 at 463.063 kb on - strand, within ABZR87_RS02160 at 463.129 kb on - strand, within ABZR87_RS02160 at 463.137 kb on + strand, within ABZR87_RS02160 at 463.137 kb on + strand, within ABZR87_RS02160 at 463.137 kb on + strand, within ABZR87_RS02160 at 463.138 kb on - strand, within ABZR87_RS02160 at 463.138 kb on - strand, within ABZR87_RS02160 at 463.577 kb on + strand at 463.661 kb on - strand, within ABZR87_RS02165 at 463.661 kb on - strand, within ABZR87_RS02165 at 463.661 kb on - strand, within ABZR87_RS02165 at 463.661 kb on - strand, within ABZR87_RS02165 at 463.663 kb on + strand, within ABZR87_RS02165 at 463.663 kb on + strand, within ABZR87_RS02165 at 463.664 kb on - strand, within ABZR87_RS02165
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 1mM remove 460,694 + -1.6 460,694 + -4.9 460,694 + -2.6 460,694 + -3.5 460,694 + -3.3 460,694 + -2.4 460,694 + -1.3 460,694 + -5.6 460,695 - -2.2 460,695 - -3.9 460,695 - -2.9 460,695 - -2.9 460,695 - -2.4 460,705 + -1.6 460,705 + -1.4 460,705 + -3.5 460,706 - -2.4 460,706 - -0.1 460,706 - -1.5 460,709 + -3.2 460,709 + -2.3 460,709 + -3.8 460,709 + -2.6 460,709 + -4.1 460,710 - -2.2 460,710 - -2.6 460,710 - -1.7 460,710 - -2.7 460,710 - -3.7 460,710 - -1.4 460,710 - -4.8 460,724 + -3.4 460,724 + -2.1 460,724 + -3.9 461,051 + ABZR87_RS02145 0.66 -2.6 461,051 + ABZR87_RS02145 0.66 -2.4 461,052 - ABZR87_RS02145 0.66 -1.5 461,101 + ABZR87_RS02145 0.75 -1.5 461,512 + ABZR87_RS02150 0.62 +0.3 461,513 - ABZR87_RS02150 0.62 -2.1 461,513 - ABZR87_RS02150 0.62 +0.5 461,513 - ABZR87_RS02150 0.62 -0.6 461,681 + -0.9 461,681 + +0.6 461,681 + -2.2 461,681 + +0.5 461,682 - -0.8 461,728 + -2.6 461,729 - -1.8 462,025 + ABZR87_RS02155 0.32 -2.1 462,025 + ABZR87_RS02155 0.32 -3.4 462,025 + ABZR87_RS02155 0.32 -1.4 462,025 + ABZR87_RS02155 0.32 -4.0 462,026 - ABZR87_RS02155 0.32 -3.9 462,447 - ABZR87_RS02155 0.73 -3.9 462,498 + ABZR87_RS02155 0.78 +1.4 462,498 + ABZR87_RS02155 0.78 -3.4 462,499 - ABZR87_RS02155 0.78 -4.1 462,499 - ABZR87_RS02155 0.78 -0.6 462,499 - ABZR87_RS02155 0.78 -0.3 462,499 - ABZR87_RS02155 0.78 -3.2 462,605 + ABZR87_RS02155 0.88 -2.1 462,605 + ABZR87_RS02155 0.88 -1.4 462,606 - ABZR87_RS02155 0.88 -3.1 462,606 - ABZR87_RS02155 0.88 -4.4 462,730 + +1.9 462,731 + +0.3 462,731 + +0.5 462,731 + -0.2 462,731 + -3.7 462,731 + +1.1 462,732 - -3.4 462,732 - -1.9 462,732 - -2.1 462,732 - -1.7 462,732 - -3.7 462,732 - -2.2 462,732 - -0.5 462,732 - -2.4 462,732 - -0.4 462,732 - -2.8 462,732 - -4.0 462,993 + ABZR87_RS02160 0.32 -2.0 462,993 + ABZR87_RS02160 0.32 -3.2 462,993 + ABZR87_RS02160 0.32 -3.1 463,063 - ABZR87_RS02160 0.41 -1.6 463,129 - ABZR87_RS02160 0.49 -1.9 463,137 + ABZR87_RS02160 0.50 -3.9 463,137 + ABZR87_RS02160 0.50 -3.9 463,137 + ABZR87_RS02160 0.50 -3.0 463,138 - ABZR87_RS02160 0.50 -3.7 463,138 - ABZR87_RS02160 0.50 -1.4 463,577 + -0.4 463,661 - ABZR87_RS02165 0.17 +0.1 463,661 - ABZR87_RS02165 0.17 -1.2 463,661 - ABZR87_RS02165 0.17 +1.6 463,661 - ABZR87_RS02165 0.17 +1.0 463,663 + ABZR87_RS02165 0.18 -0.6 463,663 + ABZR87_RS02165 0.18 +0.2 463,664 - ABZR87_RS02165 0.18 -3.4
Or see this region's nucleotide sequence