Experiment: RCH2 defined with Nickel (II) chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_558 and Pf6N2E2_559 are separated by 18 nucleotides Pf6N2E2_559 and Pf6N2E2_560 overlap by 4 nucleotides Pf6N2E2_560 and Pf6N2E2_561 overlap by 4 nucleotides
Pf6N2E2_558 - Uncharacterized protein ImpJ/VasE, at 628,613 to 629,944
_558
Pf6N2E2_559 - Inner membrane protein DotU, at 629,963 to 630,727
_559
Pf6N2E2_560 - IcmF-related protein, at 630,724 to 634,548
_560
Pf6N2E2_561 - Uncharacterized protein ImpA, at 634,545 to 635,627
_561
Position (kb)
630
631
632
633
634
635 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 629.786 kb on + strand, within Pf6N2E2_558 at 629.786 kb on + strand, within Pf6N2E2_558 at 629.787 kb on - strand, within Pf6N2E2_558 at 629.962 kb on + strand at 629.962 kb on + strand at 629.962 kb on + strand at 629.962 kb on + strand at 629.962 kb on + strand at 629.962 kb on + strand at 629.962 kb on + strand at 629.962 kb on + strand at 629.963 kb on - strand at 630.090 kb on + strand, within Pf6N2E2_559 at 630.201 kb on - strand, within Pf6N2E2_559 at 630.201 kb on - strand, within Pf6N2E2_559 at 630.293 kb on + strand, within Pf6N2E2_559 at 630.293 kb on + strand, within Pf6N2E2_559 at 630.293 kb on + strand, within Pf6N2E2_559 at 630.293 kb on + strand, within Pf6N2E2_559 at 630.293 kb on + strand, within Pf6N2E2_559 at 630.294 kb on - strand, within Pf6N2E2_559 at 630.294 kb on - strand, within Pf6N2E2_559 at 630.294 kb on - strand, within Pf6N2E2_559 at 630.294 kb on - strand, within Pf6N2E2_559 at 630.294 kb on - strand, within Pf6N2E2_559 at 630.294 kb on - strand, within Pf6N2E2_559 at 630.294 kb on - strand, within Pf6N2E2_559 at 630.294 kb on - strand, within Pf6N2E2_559 at 630.343 kb on - strand, within Pf6N2E2_559 at 630.387 kb on - strand, within Pf6N2E2_559 at 630.503 kb on + strand, within Pf6N2E2_559 at 630.503 kb on + strand, within Pf6N2E2_559 at 630.503 kb on + strand, within Pf6N2E2_559 at 630.839 kb on - strand at 631.255 kb on + strand, within Pf6N2E2_560 at 631.255 kb on + strand, within Pf6N2E2_560 at 631.255 kb on + strand, within Pf6N2E2_560 at 631.255 kb on + strand, within Pf6N2E2_560 at 631.255 kb on + strand, within Pf6N2E2_560 at 631.256 kb on - strand, within Pf6N2E2_560 at 631.256 kb on - strand, within Pf6N2E2_560 at 631.256 kb on - strand, within Pf6N2E2_560 at 631.357 kb on + strand, within Pf6N2E2_560 at 631.357 kb on + strand, within Pf6N2E2_560 at 631.358 kb on - strand, within Pf6N2E2_560 at 631.588 kb on + strand, within Pf6N2E2_560 at 631.588 kb on + strand, within Pf6N2E2_560 at 631.588 kb on + strand, within Pf6N2E2_560 at 631.588 kb on + strand, within Pf6N2E2_560 at 631.588 kb on + strand, within Pf6N2E2_560 at 631.588 kb on + strand, within Pf6N2E2_560 at 631.589 kb on - strand, within Pf6N2E2_560 at 631.589 kb on - strand, within Pf6N2E2_560 at 632.182 kb on + strand, within Pf6N2E2_560 at 632.182 kb on + strand, within Pf6N2E2_560 at 632.182 kb on + strand, within Pf6N2E2_560 at 632.182 kb on + strand, within Pf6N2E2_560 at 632.183 kb on - strand, within Pf6N2E2_560 at 632.183 kb on - strand, within Pf6N2E2_560 at 632.183 kb on - strand, within Pf6N2E2_560 at 632.183 kb on - strand, within Pf6N2E2_560 at 632.183 kb on - strand, within Pf6N2E2_560 at 632.183 kb on - strand, within Pf6N2E2_560 at 632.183 kb on - strand, within Pf6N2E2_560 at 632.183 kb on - strand, within Pf6N2E2_560 at 632.470 kb on + strand, within Pf6N2E2_560 at 632.470 kb on + strand, within Pf6N2E2_560 at 632.471 kb on - strand, within Pf6N2E2_560 at 632.986 kb on + strand, within Pf6N2E2_560 at 632.987 kb on - strand, within Pf6N2E2_560 at 632.998 kb on + strand, within Pf6N2E2_560 at 632.999 kb on - strand, within Pf6N2E2_560 at 632.999 kb on - strand, within Pf6N2E2_560 at 632.999 kb on - strand, within Pf6N2E2_560 at 633.007 kb on + strand, within Pf6N2E2_560 at 633.007 kb on + strand, within Pf6N2E2_560 at 633.008 kb on - strand, within Pf6N2E2_560 at 633.008 kb on - strand, within Pf6N2E2_560 at 633.008 kb on - strand, within Pf6N2E2_560 at 633.008 kb on - strand, within Pf6N2E2_560 at 633.008 kb on - strand, within Pf6N2E2_560 at 633.353 kb on - strand, within Pf6N2E2_560 at 633.353 kb on - strand, within Pf6N2E2_560 at 633.536 kb on - strand, within Pf6N2E2_560 at 633.536 kb on - strand, within Pf6N2E2_560 at 633.719 kb on - strand, within Pf6N2E2_560 at 633.766 kb on + strand, within Pf6N2E2_560 at 633.767 kb on - strand, within Pf6N2E2_560 at 633.767 kb on - strand, within Pf6N2E2_560 at 633.779 kb on - strand, within Pf6N2E2_560 at 633.964 kb on - strand, within Pf6N2E2_560 at 633.987 kb on - strand, within Pf6N2E2_560 at 634.261 kb on + strand at 634.333 kb on + strand at 634.334 kb on - strand at 634.823 kb on + strand, within Pf6N2E2_561 at 634.824 kb on - strand, within Pf6N2E2_561 at 635.241 kb on + strand, within Pf6N2E2_561 at 635.241 kb on + strand, within Pf6N2E2_561 at 635.242 kb on - strand, within Pf6N2E2_561 at 635.266 kb on - strand, within Pf6N2E2_561 at 635.266 kb on - strand, within Pf6N2E2_561 at 635.459 kb on - strand, within Pf6N2E2_561 at 635.459 kb on - strand, within Pf6N2E2_561 at 635.486 kb on + strand, within Pf6N2E2_561
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Nickel (II) chloride 0.04 mM remove 629,786 + Pf6N2E2_558 0.88 -1.4 629,786 + Pf6N2E2_558 0.88 +0.8 629,787 - Pf6N2E2_558 0.88 +0.8 629,962 + +1.0 629,962 + -3.2 629,962 + -0.5 629,962 + -2.2 629,962 + +0.4 629,962 + -0.6 629,962 + +0.4 629,962 + +0.5 629,963 - -2.8 630,090 + Pf6N2E2_559 0.17 -0.1 630,201 - Pf6N2E2_559 0.31 -2.1 630,201 - Pf6N2E2_559 0.31 -0.8 630,293 + Pf6N2E2_559 0.43 +1.0 630,293 + Pf6N2E2_559 0.43 -1.1 630,293 + Pf6N2E2_559 0.43 -0.1 630,293 + Pf6N2E2_559 0.43 +1.3 630,293 + Pf6N2E2_559 0.43 -1.2 630,294 - Pf6N2E2_559 0.43 -0.0 630,294 - Pf6N2E2_559 0.43 +0.5 630,294 - Pf6N2E2_559 0.43 +0.2 630,294 - Pf6N2E2_559 0.43 -0.9 630,294 - Pf6N2E2_559 0.43 -1.5 630,294 - Pf6N2E2_559 0.43 -0.7 630,294 - Pf6N2E2_559 0.43 -0.7 630,294 - Pf6N2E2_559 0.43 -3.0 630,343 - Pf6N2E2_559 0.50 -0.5 630,387 - Pf6N2E2_559 0.55 -0.6 630,503 + Pf6N2E2_559 0.71 +0.5 630,503 + Pf6N2E2_559 0.71 +1.3 630,503 + Pf6N2E2_559 0.71 +1.7 630,839 - +1.2 631,255 + Pf6N2E2_560 0.14 -2.5 631,255 + Pf6N2E2_560 0.14 -0.1 631,255 + Pf6N2E2_560 0.14 -2.1 631,255 + Pf6N2E2_560 0.14 +1.4 631,255 + Pf6N2E2_560 0.14 -0.3 631,256 - Pf6N2E2_560 0.14 +1.8 631,256 - Pf6N2E2_560 0.14 +0.1 631,256 - Pf6N2E2_560 0.14 +0.3 631,357 + Pf6N2E2_560 0.17 +1.8 631,357 + Pf6N2E2_560 0.17 +0.5 631,358 - Pf6N2E2_560 0.17 -2.7 631,588 + Pf6N2E2_560 0.23 -0.3 631,588 + Pf6N2E2_560 0.23 -0.5 631,588 + Pf6N2E2_560 0.23 +0.2 631,588 + Pf6N2E2_560 0.23 +0.6 631,588 + Pf6N2E2_560 0.23 +0.2 631,588 + Pf6N2E2_560 0.23 +0.5 631,589 - Pf6N2E2_560 0.23 +0.5 631,589 - Pf6N2E2_560 0.23 +1.2 632,182 + Pf6N2E2_560 0.38 +0.5 632,182 + Pf6N2E2_560 0.38 +2.1 632,182 + Pf6N2E2_560 0.38 +0.2 632,182 + Pf6N2E2_560 0.38 -0.6 632,183 - Pf6N2E2_560 0.38 -1.1 632,183 - Pf6N2E2_560 0.38 +1.5 632,183 - Pf6N2E2_560 0.38 -0.9 632,183 - Pf6N2E2_560 0.38 +1.1 632,183 - Pf6N2E2_560 0.38 +2.5 632,183 - Pf6N2E2_560 0.38 +0.5 632,183 - Pf6N2E2_560 0.38 +0.9 632,183 - Pf6N2E2_560 0.38 +0.7 632,470 + Pf6N2E2_560 0.46 -0.9 632,470 + Pf6N2E2_560 0.46 +1.1 632,471 - Pf6N2E2_560 0.46 +0.8 632,986 + Pf6N2E2_560 0.59 -0.5 632,987 - Pf6N2E2_560 0.59 -0.5 632,998 + Pf6N2E2_560 0.59 +1.5 632,999 - Pf6N2E2_560 0.59 -1.5 632,999 - Pf6N2E2_560 0.59 +0.3 632,999 - Pf6N2E2_560 0.59 +0.9 633,007 + Pf6N2E2_560 0.60 +0.5 633,007 + Pf6N2E2_560 0.60 -0.4 633,008 - Pf6N2E2_560 0.60 +2.3 633,008 - Pf6N2E2_560 0.60 +0.7 633,008 - Pf6N2E2_560 0.60 -0.0 633,008 - Pf6N2E2_560 0.60 -0.2 633,008 - Pf6N2E2_560 0.60 -4.1 633,353 - Pf6N2E2_560 0.69 +0.9 633,353 - Pf6N2E2_560 0.69 +1.1 633,536 - Pf6N2E2_560 0.74 -2.3 633,536 - Pf6N2E2_560 0.74 -0.5 633,719 - Pf6N2E2_560 0.78 +1.2 633,766 + Pf6N2E2_560 0.80 -1.1 633,767 - Pf6N2E2_560 0.80 +1.7 633,767 - Pf6N2E2_560 0.80 +0.6 633,779 - Pf6N2E2_560 0.80 +0.7 633,964 - Pf6N2E2_560 0.85 -0.2 633,987 - Pf6N2E2_560 0.85 +0.5 634,261 + -0.2 634,333 + +0.4 634,334 - -0.7 634,823 + Pf6N2E2_561 0.26 -0.2 634,824 - Pf6N2E2_561 0.26 -0.7 635,241 + Pf6N2E2_561 0.64 -0.2 635,241 + Pf6N2E2_561 0.64 -0.5 635,242 - Pf6N2E2_561 0.64 +0.8 635,266 - Pf6N2E2_561 0.67 -1.0 635,266 - Pf6N2E2_561 0.67 -0.0 635,459 - Pf6N2E2_561 0.84 +0.4 635,459 - Pf6N2E2_561 0.84 -0.9 635,486 + Pf6N2E2_561 0.87 +0.8
Or see this region's nucleotide sequence