Experiment: RCH2 defined with Nickel (II) chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4625 and Pf6N2E2_4626 are separated by 67 nucleotides Pf6N2E2_4626 and Pf6N2E2_4627 are separated by 1 nucleotides Pf6N2E2_4627 and Pf6N2E2_4628 are separated by 114 nucleotides
Pf6N2E2_4625 - Malonyl CoA acyl carrier protein transacylase (EC 2.3.1.39), at 5,354,225 to 5,355,145
_4625
Pf6N2E2_4626 - Malonate transporter, MadL subunit, at 5,355,213 to 5,355,629
_4626
Pf6N2E2_4627 - Malonate transporter, MadM subunit, at 5,355,631 to 5,356,395
_4627
Pf6N2E2_4628 - Malonate utilization transcriptional regulator, at 5,356,510 to 5,357,424
_4628
Position (kb)
5355
5356
5357 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5354.873 kb on + strand, within Pf6N2E2_4625 at 5354.985 kb on - strand, within Pf6N2E2_4625 at 5355.143 kb on + strand at 5355.143 kb on + strand at 5355.206 kb on + strand at 5355.206 kb on + strand at 5355.207 kb on - strand at 5355.218 kb on + strand at 5355.218 kb on + strand at 5355.218 kb on + strand at 5355.218 kb on + strand at 5355.218 kb on + strand at 5355.219 kb on - strand at 5355.222 kb on + strand at 5355.222 kb on + strand at 5355.222 kb on + strand at 5355.222 kb on + strand at 5355.223 kb on - strand at 5355.223 kb on - strand at 5355.423 kb on + strand, within Pf6N2E2_4626 at 5355.423 kb on + strand, within Pf6N2E2_4626 at 5355.423 kb on + strand, within Pf6N2E2_4626 at 5355.423 kb on + strand, within Pf6N2E2_4626 at 5355.423 kb on + strand, within Pf6N2E2_4626 at 5355.423 kb on + strand, within Pf6N2E2_4626 at 5355.423 kb on + strand, within Pf6N2E2_4626 at 5355.423 kb on + strand, within Pf6N2E2_4626 at 5355.424 kb on - strand, within Pf6N2E2_4626 at 5355.616 kb on + strand at 5355.617 kb on - strand at 5355.617 kb on - strand at 5355.617 kb on - strand at 5355.617 kb on - strand at 5355.630 kb on + strand at 5355.630 kb on + strand at 5355.630 kb on + strand at 5355.631 kb on - strand at 5355.664 kb on - strand at 5355.664 kb on - strand at 5355.664 kb on - strand at 5355.739 kb on + strand, within Pf6N2E2_4627 at 5355.739 kb on + strand, within Pf6N2E2_4627 at 5355.739 kb on + strand, within Pf6N2E2_4627 at 5355.740 kb on - strand, within Pf6N2E2_4627 at 5355.740 kb on - strand, within Pf6N2E2_4627 at 5356.072 kb on + strand, within Pf6N2E2_4627 at 5356.072 kb on + strand, within Pf6N2E2_4627 at 5356.072 kb on + strand, within Pf6N2E2_4627 at 5356.072 kb on + strand, within Pf6N2E2_4627 at 5356.072 kb on + strand, within Pf6N2E2_4627 at 5356.072 kb on + strand, within Pf6N2E2_4627 at 5356.072 kb on + strand, within Pf6N2E2_4627 at 5356.072 kb on + strand, within Pf6N2E2_4627 at 5356.072 kb on + strand, within Pf6N2E2_4627 at 5356.073 kb on - strand, within Pf6N2E2_4627 at 5356.073 kb on - strand, within Pf6N2E2_4627 at 5356.073 kb on - strand, within Pf6N2E2_4627 at 5356.073 kb on - strand, within Pf6N2E2_4627 at 5356.073 kb on - strand, within Pf6N2E2_4627 at 5356.073 kb on - strand, within Pf6N2E2_4627 at 5356.073 kb on - strand, within Pf6N2E2_4627 at 5356.073 kb on - strand, within Pf6N2E2_4627 at 5356.210 kb on - strand, within Pf6N2E2_4627 at 5356.303 kb on + strand, within Pf6N2E2_4627 at 5356.367 kb on - strand at 5356.367 kb on - strand at 5356.367 kb on - strand at 5356.367 kb on - strand at 5356.394 kb on - strand at 5356.467 kb on + strand at 5356.467 kb on + strand at 5356.468 kb on - strand at 5356.468 kb on - strand at 5356.503 kb on + strand at 5356.503 kb on + strand at 5356.503 kb on + strand at 5356.504 kb on - strand at 5356.504 kb on - strand at 5356.529 kb on + strand at 5356.529 kb on + strand at 5356.529 kb on + strand at 5356.529 kb on + strand at 5356.529 kb on + strand at 5356.529 kb on + strand at 5356.530 kb on - strand at 5356.530 kb on - strand at 5356.530 kb on - strand at 5356.530 kb on - strand at 5356.612 kb on + strand, within Pf6N2E2_4628 at 5356.612 kb on + strand, within Pf6N2E2_4628 at 5356.612 kb on + strand, within Pf6N2E2_4628 at 5356.659 kb on - strand, within Pf6N2E2_4628 at 5357.006 kb on + strand, within Pf6N2E2_4628 at 5357.006 kb on + strand, within Pf6N2E2_4628 at 5357.006 kb on + strand, within Pf6N2E2_4628 at 5357.007 kb on - strand, within Pf6N2E2_4628 at 5357.111 kb on + strand, within Pf6N2E2_4628 at 5357.171 kb on - strand, within Pf6N2E2_4628 at 5357.222 kb on + strand, within Pf6N2E2_4628 at 5357.222 kb on + strand, within Pf6N2E2_4628 at 5357.222 kb on + strand, within Pf6N2E2_4628 at 5357.222 kb on + strand, within Pf6N2E2_4628 at 5357.222 kb on + strand, within Pf6N2E2_4628 at 5357.223 kb on - strand, within Pf6N2E2_4628 at 5357.223 kb on - strand, within Pf6N2E2_4628
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Nickel (II) chloride 0.04 mM remove 5,354,873 + Pf6N2E2_4625 0.70 -0.7 5,354,985 - Pf6N2E2_4625 0.83 -2.5 5,355,143 + -0.1 5,355,143 + +0.4 5,355,206 + +0.7 5,355,206 + -0.2 5,355,207 - +0.3 5,355,218 + +0.3 5,355,218 + +1.7 5,355,218 + -0.1 5,355,218 + -0.4 5,355,218 + -0.7 5,355,219 - +0.8 5,355,222 + +0.3 5,355,222 + -0.1 5,355,222 + +0.7 5,355,222 + -0.9 5,355,223 - +1.0 5,355,223 - +1.1 5,355,423 + Pf6N2E2_4626 0.50 +0.3 5,355,423 + Pf6N2E2_4626 0.50 -0.6 5,355,423 + Pf6N2E2_4626 0.50 -1.6 5,355,423 + Pf6N2E2_4626 0.50 -1.7 5,355,423 + Pf6N2E2_4626 0.50 +0.7 5,355,423 + Pf6N2E2_4626 0.50 +0.6 5,355,423 + Pf6N2E2_4626 0.50 +0.1 5,355,423 + Pf6N2E2_4626 0.50 +0.6 5,355,424 - Pf6N2E2_4626 0.51 +0.1 5,355,616 + -0.1 5,355,617 - +0.2 5,355,617 - +0.9 5,355,617 - +0.0 5,355,617 - -0.2 5,355,630 + -0.6 5,355,630 + -0.3 5,355,630 + -0.0 5,355,631 - +1.7 5,355,664 - -2.9 5,355,664 - +0.8 5,355,664 - +0.6 5,355,739 + Pf6N2E2_4627 0.14 -0.1 5,355,739 + Pf6N2E2_4627 0.14 -1.6 5,355,739 + Pf6N2E2_4627 0.14 +2.4 5,355,740 - Pf6N2E2_4627 0.14 +1.9 5,355,740 - Pf6N2E2_4627 0.14 +0.9 5,356,072 + Pf6N2E2_4627 0.58 +1.1 5,356,072 + Pf6N2E2_4627 0.58 +1.4 5,356,072 + Pf6N2E2_4627 0.58 +1.0 5,356,072 + Pf6N2E2_4627 0.58 -0.8 5,356,072 + Pf6N2E2_4627 0.58 +1.9 5,356,072 + Pf6N2E2_4627 0.58 -0.1 5,356,072 + Pf6N2E2_4627 0.58 -0.8 5,356,072 + Pf6N2E2_4627 0.58 +0.4 5,356,072 + Pf6N2E2_4627 0.58 +1.6 5,356,073 - Pf6N2E2_4627 0.58 -0.9 5,356,073 - Pf6N2E2_4627 0.58 -1.7 5,356,073 - Pf6N2E2_4627 0.58 +0.9 5,356,073 - Pf6N2E2_4627 0.58 +1.2 5,356,073 - Pf6N2E2_4627 0.58 -0.0 5,356,073 - Pf6N2E2_4627 0.58 +0.7 5,356,073 - Pf6N2E2_4627 0.58 -0.2 5,356,073 - Pf6N2E2_4627 0.58 +0.0 5,356,210 - Pf6N2E2_4627 0.76 +1.4 5,356,303 + Pf6N2E2_4627 0.88 -0.1 5,356,367 - +0.4 5,356,367 - +1.6 5,356,367 - +0.4 5,356,367 - -0.6 5,356,394 - -0.5 5,356,467 + +0.0 5,356,467 + -0.2 5,356,468 - +0.9 5,356,468 - -0.2 5,356,503 + -0.4 5,356,503 + -1.1 5,356,503 + +0.7 5,356,504 - -0.8 5,356,504 - +1.0 5,356,529 + -1.3 5,356,529 + +0.7 5,356,529 + +1.4 5,356,529 + +1.4 5,356,529 + +0.4 5,356,529 + -0.6 5,356,530 - +0.6 5,356,530 - +1.9 5,356,530 - +1.4 5,356,530 - +0.9 5,356,612 + Pf6N2E2_4628 0.11 +1.0 5,356,612 + Pf6N2E2_4628 0.11 -0.2 5,356,612 + Pf6N2E2_4628 0.11 +0.6 5,356,659 - Pf6N2E2_4628 0.16 -1.9 5,357,006 + Pf6N2E2_4628 0.54 +0.2 5,357,006 + Pf6N2E2_4628 0.54 +1.2 5,357,006 + Pf6N2E2_4628 0.54 -2.9 5,357,007 - Pf6N2E2_4628 0.54 -0.9 5,357,111 + Pf6N2E2_4628 0.66 -0.9 5,357,171 - Pf6N2E2_4628 0.72 -0.1 5,357,222 + Pf6N2E2_4628 0.78 -0.7 5,357,222 + Pf6N2E2_4628 0.78 -0.1 5,357,222 + Pf6N2E2_4628 0.78 -1.1 5,357,222 + Pf6N2E2_4628 0.78 +0.0 5,357,222 + Pf6N2E2_4628 0.78 +1.1 5,357,223 - Pf6N2E2_4628 0.78 +0.4 5,357,223 - Pf6N2E2_4628 0.78 +1.7
Or see this region's nucleotide sequence