Experiment: RCH2 defined with Nickel (II) chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4376 and Pf6N2E2_4377 are separated by 144 nucleotides Pf6N2E2_4377 and Pf6N2E2_4378 are separated by 77 nucleotides Pf6N2E2_4378 and Pf6N2E2_4379 are separated by 473 nucleotides Pf6N2E2_4379 and Pf6N2E2_4380 are separated by 11 nucleotides Pf6N2E2_4380 and Pf6N2E2_4381 are separated by 90 nucleotides
Pf6N2E2_4376 - Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36) / Phosphopantothenoylcysteine synthetase (EC 6.3.2.5), at 5,104,857 to 5,106,065
_4376
Pf6N2E2_4377 - DNA repair protein RadC, at 5,106,210 to 5,106,884
_4377
Pf6N2E2_4378 - Dipeptide-binding ABC transporter, periplasmic substrate-binding component (TC 3.A.1.5.2), at 5,106,962 to 5,108,551
_4378
Pf6N2E2_4379 - LSU ribosomal protein L28p, at 5,109,025 to 5,109,222
_4379
Pf6N2E2_4380 - LSU ribosomal protein L33p @ LSU ribosomal protein L33p, zinc-independent, at 5,109,234 to 5,109,389
_4380
Pf6N2E2_4381 - hypothetical protein, at 5,109,480 to 5,109,698
_4381
Position (kb)
5106
5107
5108
5109 Strain fitness (log2 ratio)
-1
0
1
2 at 5106.066 kb on - strand at 5106.156 kb on - strand at 5106.156 kb on - strand at 5106.215 kb on + strand at 5106.215 kb on + strand at 5106.215 kb on + strand at 5106.216 kb on - strand at 5106.216 kb on - strand at 5106.216 kb on - strand at 5106.281 kb on - strand, within Pf6N2E2_4377 at 5106.358 kb on + strand, within Pf6N2E2_4377 at 5106.359 kb on - strand, within Pf6N2E2_4377 at 5106.359 kb on - strand, within Pf6N2E2_4377 at 5106.359 kb on - strand, within Pf6N2E2_4377 at 5106.359 kb on - strand, within Pf6N2E2_4377 at 5106.359 kb on - strand, within Pf6N2E2_4377 at 5106.359 kb on - strand, within Pf6N2E2_4377 at 5106.359 kb on - strand, within Pf6N2E2_4377 at 5106.359 kb on - strand, within Pf6N2E2_4377 at 5106.359 kb on - strand, within Pf6N2E2_4377 at 5106.422 kb on + strand, within Pf6N2E2_4377 at 5106.422 kb on + strand, within Pf6N2E2_4377 at 5106.423 kb on - strand, within Pf6N2E2_4377 at 5106.544 kb on - strand, within Pf6N2E2_4377 at 5106.963 kb on + strand at 5106.987 kb on + strand at 5106.988 kb on - strand at 5106.988 kb on - strand at 5107.113 kb on + strand at 5107.113 kb on + strand at 5107.114 kb on - strand at 5107.114 kb on - strand at 5107.540 kb on - strand, within Pf6N2E2_4378 at 5107.609 kb on - strand, within Pf6N2E2_4378 at 5107.732 kb on + strand, within Pf6N2E2_4378 at 5107.732 kb on + strand, within Pf6N2E2_4378 at 5107.732 kb on + strand, within Pf6N2E2_4378 at 5107.733 kb on - strand, within Pf6N2E2_4378 at 5107.733 kb on - strand, within Pf6N2E2_4378 at 5107.733 kb on - strand, within Pf6N2E2_4378 at 5107.733 kb on - strand, within Pf6N2E2_4378 at 5107.733 kb on - strand, within Pf6N2E2_4378 at 5107.733 kb on - strand, within Pf6N2E2_4378 at 5107.754 kb on + strand, within Pf6N2E2_4378 at 5107.754 kb on + strand, within Pf6N2E2_4378 at 5107.754 kb on + strand, within Pf6N2E2_4378 at 5107.754 kb on + strand, within Pf6N2E2_4378 at 5107.754 kb on + strand, within Pf6N2E2_4378 at 5107.754 kb on + strand, within Pf6N2E2_4378 at 5107.755 kb on - strand, within Pf6N2E2_4378 at 5107.755 kb on - strand, within Pf6N2E2_4378 at 5107.755 kb on - strand, within Pf6N2E2_4378 at 5107.755 kb on - strand, within Pf6N2E2_4378 at 5107.755 kb on - strand, within Pf6N2E2_4378 at 5107.878 kb on + strand, within Pf6N2E2_4378 at 5107.879 kb on - strand, within Pf6N2E2_4378 at 5107.896 kb on + strand, within Pf6N2E2_4378 at 5107.896 kb on + strand, within Pf6N2E2_4378 at 5107.896 kb on + strand, within Pf6N2E2_4378 at 5107.896 kb on + strand, within Pf6N2E2_4378 at 5107.897 kb on - strand, within Pf6N2E2_4378 at 5107.897 kb on - strand, within Pf6N2E2_4378 at 5107.897 kb on - strand, within Pf6N2E2_4378 at 5108.005 kb on - strand, within Pf6N2E2_4378 at 5108.016 kb on + strand, within Pf6N2E2_4378 at 5108.017 kb on - strand, within Pf6N2E2_4378 at 5108.017 kb on - strand, within Pf6N2E2_4378 at 5108.257 kb on - strand, within Pf6N2E2_4378 at 5108.257 kb on - strand, within Pf6N2E2_4378 at 5108.301 kb on + strand, within Pf6N2E2_4378 at 5108.301 kb on + strand, within Pf6N2E2_4378 at 5108.301 kb on + strand, within Pf6N2E2_4378 at 5108.301 kb on + strand, within Pf6N2E2_4378 at 5108.302 kb on - strand, within Pf6N2E2_4378 at 5108.302 kb on - strand, within Pf6N2E2_4378 at 5108.302 kb on - strand, within Pf6N2E2_4378 at 5108.302 kb on - strand, within Pf6N2E2_4378 at 5108.302 kb on - strand, within Pf6N2E2_4378 at 5108.302 kb on - strand, within Pf6N2E2_4378 at 5108.302 kb on - strand, within Pf6N2E2_4378 at 5108.302 kb on - strand, within Pf6N2E2_4378 at 5108.302 kb on - strand, within Pf6N2E2_4378 at 5108.302 kb on - strand, within Pf6N2E2_4378 at 5108.302 kb on - strand, within Pf6N2E2_4378 at 5108.302 kb on - strand, within Pf6N2E2_4378 at 5108.302 kb on - strand, within Pf6N2E2_4378 at 5108.302 kb on - strand, within Pf6N2E2_4378 at 5108.433 kb on + strand at 5108.433 kb on + strand at 5108.433 kb on + strand at 5108.433 kb on + strand at 5108.434 kb on - strand at 5108.434 kb on - strand at 5108.434 kb on - strand at 5108.434 kb on - strand at 5108.434 kb on - strand at 5108.434 kb on - strand at 5108.434 kb on - strand at 5108.434 kb on - strand at 5108.613 kb on + strand at 5108.613 kb on + strand at 5108.731 kb on + strand at 5108.731 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Nickel (II) chloride 0.04 mM remove 5,106,066 - -1.1 5,106,156 - +0.4 5,106,156 - +0.1 5,106,215 + +0.9 5,106,215 + +0.1 5,106,215 + +0.5 5,106,216 - +1.1 5,106,216 - +0.1 5,106,216 - +0.2 5,106,281 - Pf6N2E2_4377 0.11 -0.3 5,106,358 + Pf6N2E2_4377 0.22 +0.5 5,106,359 - Pf6N2E2_4377 0.22 -0.9 5,106,359 - Pf6N2E2_4377 0.22 +1.4 5,106,359 - Pf6N2E2_4377 0.22 +0.2 5,106,359 - Pf6N2E2_4377 0.22 +0.1 5,106,359 - Pf6N2E2_4377 0.22 -1.5 5,106,359 - Pf6N2E2_4377 0.22 -0.1 5,106,359 - Pf6N2E2_4377 0.22 -1.0 5,106,359 - Pf6N2E2_4377 0.22 +0.7 5,106,359 - Pf6N2E2_4377 0.22 -0.8 5,106,422 + Pf6N2E2_4377 0.31 +0.8 5,106,422 + Pf6N2E2_4377 0.31 +1.4 5,106,423 - Pf6N2E2_4377 0.32 -0.2 5,106,544 - Pf6N2E2_4377 0.49 +0.9 5,106,963 + +0.9 5,106,987 + +1.7 5,106,988 - +1.6 5,106,988 - +1.3 5,107,113 + +2.0 5,107,113 + +0.4 5,107,114 - +1.2 5,107,114 - +1.7 5,107,540 - Pf6N2E2_4378 0.36 +1.1 5,107,609 - Pf6N2E2_4378 0.41 +1.3 5,107,732 + Pf6N2E2_4378 0.48 +0.6 5,107,732 + Pf6N2E2_4378 0.48 +2.6 5,107,732 + Pf6N2E2_4378 0.48 +1.0 5,107,733 - Pf6N2E2_4378 0.48 +1.4 5,107,733 - Pf6N2E2_4378 0.48 +1.1 5,107,733 - Pf6N2E2_4378 0.48 -0.8 5,107,733 - Pf6N2E2_4378 0.48 +1.5 5,107,733 - Pf6N2E2_4378 0.48 +2.5 5,107,733 - Pf6N2E2_4378 0.48 +0.5 5,107,754 + Pf6N2E2_4378 0.50 +2.3 5,107,754 + Pf6N2E2_4378 0.50 +1.3 5,107,754 + Pf6N2E2_4378 0.50 +1.7 5,107,754 + Pf6N2E2_4378 0.50 +1.4 5,107,754 + Pf6N2E2_4378 0.50 +2.1 5,107,754 + Pf6N2E2_4378 0.50 +1.3 5,107,755 - Pf6N2E2_4378 0.50 +1.3 5,107,755 - Pf6N2E2_4378 0.50 +2.1 5,107,755 - Pf6N2E2_4378 0.50 +1.5 5,107,755 - Pf6N2E2_4378 0.50 +1.3 5,107,755 - Pf6N2E2_4378 0.50 +1.2 5,107,878 + Pf6N2E2_4378 0.58 +1.8 5,107,879 - Pf6N2E2_4378 0.58 +1.2 5,107,896 + Pf6N2E2_4378 0.59 +1.0 5,107,896 + Pf6N2E2_4378 0.59 +1.5 5,107,896 + Pf6N2E2_4378 0.59 +1.9 5,107,896 + Pf6N2E2_4378 0.59 +0.9 5,107,897 - Pf6N2E2_4378 0.59 +1.2 5,107,897 - Pf6N2E2_4378 0.59 +1.6 5,107,897 - Pf6N2E2_4378 0.59 +2.0 5,108,005 - Pf6N2E2_4378 0.66 +2.1 5,108,016 + Pf6N2E2_4378 0.66 +1.9 5,108,017 - Pf6N2E2_4378 0.66 -0.4 5,108,017 - Pf6N2E2_4378 0.66 +1.8 5,108,257 - Pf6N2E2_4378 0.81 +0.9 5,108,257 - Pf6N2E2_4378 0.81 +1.5 5,108,301 + Pf6N2E2_4378 0.84 +1.0 5,108,301 + Pf6N2E2_4378 0.84 +1.5 5,108,301 + Pf6N2E2_4378 0.84 +1.2 5,108,301 + Pf6N2E2_4378 0.84 +1.6 5,108,302 - Pf6N2E2_4378 0.84 +1.6 5,108,302 - Pf6N2E2_4378 0.84 +2.3 5,108,302 - Pf6N2E2_4378 0.84 +1.2 5,108,302 - Pf6N2E2_4378 0.84 +1.2 5,108,302 - Pf6N2E2_4378 0.84 +1.2 5,108,302 - Pf6N2E2_4378 0.84 +0.3 5,108,302 - Pf6N2E2_4378 0.84 +1.7 5,108,302 - Pf6N2E2_4378 0.84 +1.3 5,108,302 - Pf6N2E2_4378 0.84 +1.1 5,108,302 - Pf6N2E2_4378 0.84 +2.1 5,108,302 - Pf6N2E2_4378 0.84 +1.7 5,108,302 - Pf6N2E2_4378 0.84 +1.0 5,108,302 - Pf6N2E2_4378 0.84 +1.2 5,108,302 - Pf6N2E2_4378 0.84 +0.1 5,108,433 + +1.4 5,108,433 + +1.4 5,108,433 + +1.2 5,108,433 + +2.3 5,108,434 - +1.4 5,108,434 - +1.2 5,108,434 - +2.5 5,108,434 - +0.1 5,108,434 - +2.6 5,108,434 - +1.7 5,108,434 - +0.6 5,108,434 - +0.7 5,108,613 + +1.8 5,108,613 + +1.6 5,108,731 + -0.4 5,108,731 + +1.5
Or see this region's nucleotide sequence