Experiment: RCH2 defined with Nickel (II) chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3422 and Pf6N2E2_3423 are separated by 3 nucleotides Pf6N2E2_3423 and Pf6N2E2_3424 overlap by 4 nucleotides Pf6N2E2_3424 and Pf6N2E2_3425 overlap by 4 nucleotides Pf6N2E2_3425 and Pf6N2E2_3426 overlap by 4 nucleotides
Pf6N2E2_3422 - Proline iminopeptidase (EC 3.4.11.5), at 3,979,270 to 3,980,160
_3422
Pf6N2E2_3423 - Putative glutathione transporter, permease component, at 3,980,164 to 3,981,111
_3423
Pf6N2E2_3424 - Dipeptide transport system permease protein DppC (TC 3.A.1.5.2), at 3,981,108 to 3,981,965
_3424
Pf6N2E2_3425 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 3,981,962 to 3,982,948
_3425
Pf6N2E2_3426 - Peptide ABC transporter, ATP-binding protein, at 3,982,945 to 3,983,916
_3426
Position (kb)
3981
3982 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3980.116 kb on + strand at 3980.117 kb on - strand at 3980.159 kb on - strand at 3980.189 kb on + strand at 3980.190 kb on - strand at 3980.205 kb on - strand at 3980.210 kb on + strand at 3980.210 kb on + strand at 3980.211 kb on - strand at 3980.211 kb on - strand at 3980.339 kb on - strand, within Pf6N2E2_3423 at 3980.368 kb on + strand, within Pf6N2E2_3423 at 3980.368 kb on + strand, within Pf6N2E2_3423 at 3980.369 kb on - strand, within Pf6N2E2_3423 at 3980.369 kb on - strand, within Pf6N2E2_3423 at 3980.374 kb on + strand, within Pf6N2E2_3423 at 3980.374 kb on + strand, within Pf6N2E2_3423 at 3980.374 kb on + strand, within Pf6N2E2_3423 at 3980.375 kb on - strand, within Pf6N2E2_3423 at 3980.375 kb on - strand, within Pf6N2E2_3423 at 3980.375 kb on - strand, within Pf6N2E2_3423 at 3980.422 kb on + strand, within Pf6N2E2_3423 at 3980.423 kb on - strand, within Pf6N2E2_3423 at 3980.451 kb on + strand, within Pf6N2E2_3423 at 3980.790 kb on - strand, within Pf6N2E2_3423 at 3980.790 kb on - strand, within Pf6N2E2_3423 at 3980.790 kb on - strand, within Pf6N2E2_3423 at 3980.844 kb on - strand, within Pf6N2E2_3423 at 3980.978 kb on + strand, within Pf6N2E2_3423 at 3980.992 kb on - strand, within Pf6N2E2_3423 at 3981.001 kb on + strand, within Pf6N2E2_3423 at 3981.001 kb on + strand, within Pf6N2E2_3423 at 3981.001 kb on + strand, within Pf6N2E2_3423 at 3981.001 kb on + strand, within Pf6N2E2_3423 at 3981.002 kb on - strand, within Pf6N2E2_3423 at 3981.002 kb on - strand, within Pf6N2E2_3423 at 3981.002 kb on - strand, within Pf6N2E2_3423 at 3981.002 kb on - strand, within Pf6N2E2_3423 at 3981.441 kb on + strand, within Pf6N2E2_3424 at 3981.441 kb on + strand, within Pf6N2E2_3424 at 3981.442 kb on - strand, within Pf6N2E2_3424 at 3981.442 kb on - strand, within Pf6N2E2_3424 at 3981.486 kb on - strand, within Pf6N2E2_3424 at 3981.486 kb on - strand, within Pf6N2E2_3424 at 3981.488 kb on + strand, within Pf6N2E2_3424 at 3981.488 kb on + strand, within Pf6N2E2_3424 at 3981.488 kb on + strand, within Pf6N2E2_3424 at 3981.489 kb on - strand, within Pf6N2E2_3424 at 3981.561 kb on + strand, within Pf6N2E2_3424 at 3981.561 kb on + strand, within Pf6N2E2_3424 at 3981.561 kb on + strand, within Pf6N2E2_3424 at 3981.562 kb on - strand, within Pf6N2E2_3424 at 3981.562 kb on - strand, within Pf6N2E2_3424 at 3981.564 kb on - strand, within Pf6N2E2_3424 at 3981.564 kb on - strand, within Pf6N2E2_3424 at 3981.594 kb on + strand, within Pf6N2E2_3424 at 3981.595 kb on - strand, within Pf6N2E2_3424 at 3981.757 kb on + strand, within Pf6N2E2_3424 at 3981.757 kb on + strand, within Pf6N2E2_3424 at 3982.317 kb on + strand, within Pf6N2E2_3425 at 3982.317 kb on + strand, within Pf6N2E2_3425 at 3982.317 kb on + strand, within Pf6N2E2_3425 at 3982.318 kb on - strand, within Pf6N2E2_3425 at 3982.318 kb on - strand, within Pf6N2E2_3425 at 3982.419 kb on - strand, within Pf6N2E2_3425 at 3982.533 kb on + strand, within Pf6N2E2_3425 at 3982.534 kb on - strand, within Pf6N2E2_3425 at 3982.653 kb on - strand, within Pf6N2E2_3425 at 3982.653 kb on - strand, within Pf6N2E2_3425 at 3982.653 kb on - strand, within Pf6N2E2_3425 at 3982.716 kb on - strand, within Pf6N2E2_3425 at 3982.716 kb on - strand, within Pf6N2E2_3425 at 3982.716 kb on - strand, within Pf6N2E2_3425
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Nickel (II) chloride 0.04 mM remove 3,980,116 + +0.0 3,980,117 - +0.5 3,980,159 - +1.2 3,980,189 + +0.2 3,980,190 - +0.2 3,980,205 - +1.1 3,980,210 + -0.1 3,980,210 + +1.4 3,980,211 - +1.0 3,980,211 - +0.7 3,980,339 - Pf6N2E2_3423 0.18 -0.4 3,980,368 + Pf6N2E2_3423 0.22 +1.2 3,980,368 + Pf6N2E2_3423 0.22 -1.1 3,980,369 - Pf6N2E2_3423 0.22 -1.8 3,980,369 - Pf6N2E2_3423 0.22 -0.1 3,980,374 + Pf6N2E2_3423 0.22 -0.4 3,980,374 + Pf6N2E2_3423 0.22 +0.6 3,980,374 + Pf6N2E2_3423 0.22 -0.5 3,980,375 - Pf6N2E2_3423 0.22 -1.6 3,980,375 - Pf6N2E2_3423 0.22 +1.1 3,980,375 - Pf6N2E2_3423 0.22 +2.1 3,980,422 + Pf6N2E2_3423 0.27 +1.1 3,980,423 - Pf6N2E2_3423 0.27 +1.2 3,980,451 + Pf6N2E2_3423 0.30 -0.6 3,980,790 - Pf6N2E2_3423 0.66 -3.7 3,980,790 - Pf6N2E2_3423 0.66 +0.7 3,980,790 - Pf6N2E2_3423 0.66 -0.0 3,980,844 - Pf6N2E2_3423 0.72 -1.1 3,980,978 + Pf6N2E2_3423 0.86 +0.4 3,980,992 - Pf6N2E2_3423 0.87 -0.0 3,981,001 + Pf6N2E2_3423 0.88 -0.1 3,981,001 + Pf6N2E2_3423 0.88 +0.8 3,981,001 + Pf6N2E2_3423 0.88 +1.6 3,981,001 + Pf6N2E2_3423 0.88 -0.0 3,981,002 - Pf6N2E2_3423 0.88 +0.9 3,981,002 - Pf6N2E2_3423 0.88 +0.9 3,981,002 - Pf6N2E2_3423 0.88 -1.3 3,981,002 - Pf6N2E2_3423 0.88 +0.2 3,981,441 + Pf6N2E2_3424 0.39 +0.3 3,981,441 + Pf6N2E2_3424 0.39 -2.7 3,981,442 - Pf6N2E2_3424 0.39 -0.9 3,981,442 - Pf6N2E2_3424 0.39 +0.8 3,981,486 - Pf6N2E2_3424 0.44 -1.8 3,981,486 - Pf6N2E2_3424 0.44 -0.4 3,981,488 + Pf6N2E2_3424 0.44 -1.3 3,981,488 + Pf6N2E2_3424 0.44 +1.1 3,981,488 + Pf6N2E2_3424 0.44 +0.3 3,981,489 - Pf6N2E2_3424 0.44 +0.7 3,981,561 + Pf6N2E2_3424 0.53 -0.1 3,981,561 + Pf6N2E2_3424 0.53 -0.4 3,981,561 + Pf6N2E2_3424 0.53 +0.8 3,981,562 - Pf6N2E2_3424 0.53 +1.2 3,981,562 - Pf6N2E2_3424 0.53 -0.0 3,981,564 - Pf6N2E2_3424 0.53 +1.5 3,981,564 - Pf6N2E2_3424 0.53 +0.4 3,981,594 + Pf6N2E2_3424 0.57 +0.0 3,981,595 - Pf6N2E2_3424 0.57 +0.9 3,981,757 + Pf6N2E2_3424 0.76 +2.2 3,981,757 + Pf6N2E2_3424 0.76 +0.7 3,982,317 + Pf6N2E2_3425 0.36 +0.3 3,982,317 + Pf6N2E2_3425 0.36 +0.5 3,982,317 + Pf6N2E2_3425 0.36 +0.7 3,982,318 - Pf6N2E2_3425 0.36 -0.5 3,982,318 - Pf6N2E2_3425 0.36 +0.6 3,982,419 - Pf6N2E2_3425 0.46 +0.4 3,982,533 + Pf6N2E2_3425 0.58 +0.9 3,982,534 - Pf6N2E2_3425 0.58 -0.4 3,982,653 - Pf6N2E2_3425 0.70 +0.5 3,982,653 - Pf6N2E2_3425 0.70 -0.9 3,982,653 - Pf6N2E2_3425 0.70 -1.0 3,982,716 - Pf6N2E2_3425 0.76 +1.7 3,982,716 - Pf6N2E2_3425 0.76 +0.3 3,982,716 - Pf6N2E2_3425 0.76 -1.1
Or see this region's nucleotide sequence