Experiment: RCH2 defined with Nickel (II) chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3402 and Pf6N2E2_3403 overlap by 1 nucleotides Pf6N2E2_3403 and Pf6N2E2_3404 are separated by 4 nucleotides Pf6N2E2_3404 and Pf6N2E2_3405 overlap by 4 nucleotides
Pf6N2E2_3402 - D-amino acid dehydrogenase small subunit (EC 1.4.99.1), at 3,946,353 to 3,947,585
_3402
Pf6N2E2_3403 - Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7), at 3,947,585 to 3,949,012
_3403
Pf6N2E2_3404 - Multi antimicrobial extrusion protein (Na(+)/drug antiporter), MATE family of MDR efflux pumps, at 3,949,017 to 3,949,700
_3404
Pf6N2E2_3405 - FIG00984945: hypothetical protein, at 3,949,697 to 3,950,353
_3405
Position (kb)
3947
3948
3949
3950 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3946.674 kb on - strand, within Pf6N2E2_3402 at 3946.674 kb on - strand, within Pf6N2E2_3402 at 3946.733 kb on + strand, within Pf6N2E2_3402 at 3946.733 kb on + strand, within Pf6N2E2_3402 at 3946.733 kb on + strand, within Pf6N2E2_3402 at 3946.734 kb on - strand, within Pf6N2E2_3402 at 3946.734 kb on - strand, within Pf6N2E2_3402 at 3946.734 kb on - strand, within Pf6N2E2_3402 at 3946.734 kb on - strand, within Pf6N2E2_3402 at 3946.742 kb on - strand, within Pf6N2E2_3402 at 3946.785 kb on + strand, within Pf6N2E2_3402 at 3946.786 kb on - strand, within Pf6N2E2_3402 at 3946.786 kb on - strand, within Pf6N2E2_3402 at 3946.786 kb on - strand, within Pf6N2E2_3402 at 3946.802 kb on - strand, within Pf6N2E2_3402 at 3947.036 kb on + strand, within Pf6N2E2_3402 at 3947.036 kb on + strand, within Pf6N2E2_3402 at 3947.037 kb on - strand, within Pf6N2E2_3402 at 3947.037 kb on - strand, within Pf6N2E2_3402 at 3947.037 kb on - strand, within Pf6N2E2_3402 at 3947.037 kb on - strand, within Pf6N2E2_3402 at 3947.037 kb on - strand, within Pf6N2E2_3402 at 3947.039 kb on - strand, within Pf6N2E2_3402 at 3947.294 kb on + strand, within Pf6N2E2_3402 at 3947.295 kb on - strand, within Pf6N2E2_3402 at 3947.295 kb on - strand, within Pf6N2E2_3402 at 3947.434 kb on + strand, within Pf6N2E2_3402 at 3947.434 kb on + strand, within Pf6N2E2_3402 at 3947.434 kb on + strand, within Pf6N2E2_3402 at 3947.434 kb on + strand, within Pf6N2E2_3402 at 3947.434 kb on + strand, within Pf6N2E2_3402 at 3947.435 kb on - strand, within Pf6N2E2_3402 at 3947.435 kb on - strand, within Pf6N2E2_3402 at 3947.435 kb on - strand, within Pf6N2E2_3402 at 3947.435 kb on - strand, within Pf6N2E2_3402 at 3947.435 kb on - strand, within Pf6N2E2_3402 at 3947.435 kb on - strand, within Pf6N2E2_3402 at 3947.441 kb on - strand, within Pf6N2E2_3402 at 3947.441 kb on - strand, within Pf6N2E2_3402 at 3947.441 kb on - strand, within Pf6N2E2_3402 at 3947.513 kb on + strand at 3947.514 kb on - strand at 3947.514 kb on - strand at 3947.530 kb on - strand at 3947.656 kb on - strand at 3947.656 kb on - strand at 3947.814 kb on + strand, within Pf6N2E2_3403 at 3947.814 kb on + strand, within Pf6N2E2_3403 at 3947.815 kb on - strand, within Pf6N2E2_3403 at 3947.815 kb on - strand, within Pf6N2E2_3403 at 3947.815 kb on - strand, within Pf6N2E2_3403 at 3947.940 kb on + strand, within Pf6N2E2_3403 at 3947.940 kb on + strand, within Pf6N2E2_3403 at 3947.940 kb on + strand, within Pf6N2E2_3403 at 3947.940 kb on + strand, within Pf6N2E2_3403 at 3947.940 kb on + strand, within Pf6N2E2_3403 at 3947.940 kb on + strand, within Pf6N2E2_3403 at 3947.941 kb on - strand, within Pf6N2E2_3403 at 3947.941 kb on - strand, within Pf6N2E2_3403 at 3947.941 kb on - strand, within Pf6N2E2_3403 at 3948.002 kb on + strand, within Pf6N2E2_3403 at 3948.002 kb on + strand, within Pf6N2E2_3403 at 3948.003 kb on - strand, within Pf6N2E2_3403 at 3948.003 kb on - strand, within Pf6N2E2_3403 at 3948.003 kb on - strand, within Pf6N2E2_3403 at 3948.039 kb on + strand, within Pf6N2E2_3403 at 3948.040 kb on - strand, within Pf6N2E2_3403 at 3948.056 kb on - strand, within Pf6N2E2_3403 at 3948.056 kb on - strand, within Pf6N2E2_3403 at 3948.156 kb on + strand, within Pf6N2E2_3403 at 3948.156 kb on + strand, within Pf6N2E2_3403 at 3948.157 kb on - strand, within Pf6N2E2_3403 at 3948.157 kb on - strand, within Pf6N2E2_3403 at 3948.157 kb on - strand, within Pf6N2E2_3403 at 3948.157 kb on - strand, within Pf6N2E2_3403 at 3948.157 kb on - strand, within Pf6N2E2_3403 at 3948.168 kb on - strand, within Pf6N2E2_3403 at 3948.168 kb on - strand, within Pf6N2E2_3403 at 3948.215 kb on - strand, within Pf6N2E2_3403 at 3948.215 kb on - strand, within Pf6N2E2_3403 at 3948.347 kb on - strand, within Pf6N2E2_3403 at 3948.554 kb on + strand, within Pf6N2E2_3403 at 3948.554 kb on + strand, within Pf6N2E2_3403 at 3948.554 kb on + strand, within Pf6N2E2_3403 at 3948.554 kb on + strand, within Pf6N2E2_3403 at 3948.554 kb on + strand, within Pf6N2E2_3403 at 3948.554 kb on + strand, within Pf6N2E2_3403 at 3948.554 kb on + strand, within Pf6N2E2_3403 at 3948.555 kb on - strand, within Pf6N2E2_3403 at 3948.555 kb on - strand, within Pf6N2E2_3403 at 3948.555 kb on - strand, within Pf6N2E2_3403 at 3948.555 kb on - strand, within Pf6N2E2_3403 at 3948.873 kb on + strand at 3948.873 kb on + strand at 3948.873 kb on + strand at 3948.873 kb on + strand at 3948.873 kb on + strand at 3948.874 kb on - strand at 3948.874 kb on - strand at 3948.979 kb on + strand at 3948.979 kb on + strand at 3948.980 kb on - strand at 3948.980 kb on - strand at 3949.102 kb on + strand, within Pf6N2E2_3404 at 3949.103 kb on - strand, within Pf6N2E2_3404 at 3949.103 kb on - strand, within Pf6N2E2_3404 at 3949.103 kb on - strand, within Pf6N2E2_3404 at 3949.103 kb on - strand, within Pf6N2E2_3404 at 3949.103 kb on - strand, within Pf6N2E2_3404 at 3949.127 kb on + strand, within Pf6N2E2_3404 at 3949.127 kb on + strand, within Pf6N2E2_3404 at 3949.127 kb on + strand, within Pf6N2E2_3404 at 3949.128 kb on - strand, within Pf6N2E2_3404 at 3949.128 kb on - strand, within Pf6N2E2_3404 at 3949.231 kb on + strand, within Pf6N2E2_3404 at 3949.231 kb on + strand, within Pf6N2E2_3404 at 3949.231 kb on + strand, within Pf6N2E2_3404 at 3949.231 kb on + strand, within Pf6N2E2_3404 at 3949.232 kb on - strand, within Pf6N2E2_3404 at 3949.232 kb on - strand, within Pf6N2E2_3404 at 3949.232 kb on - strand, within Pf6N2E2_3404 at 3949.232 kb on - strand, within Pf6N2E2_3404 at 3949.384 kb on + strand, within Pf6N2E2_3404 at 3949.384 kb on + strand, within Pf6N2E2_3404 at 3949.384 kb on + strand, within Pf6N2E2_3404 at 3949.385 kb on - strand, within Pf6N2E2_3404 at 3949.385 kb on - strand, within Pf6N2E2_3404 at 3949.669 kb on + strand at 3949.767 kb on + strand, within Pf6N2E2_3405 at 3949.767 kb on + strand, within Pf6N2E2_3405 at 3949.767 kb on + strand, within Pf6N2E2_3405 at 3949.767 kb on + strand, within Pf6N2E2_3405 at 3949.768 kb on - strand, within Pf6N2E2_3405 at 3949.768 kb on - strand, within Pf6N2E2_3405 at 3949.768 kb on - strand, within Pf6N2E2_3405 at 3949.768 kb on - strand, within Pf6N2E2_3405 at 3949.768 kb on - strand, within Pf6N2E2_3405 at 3949.788 kb on + strand, within Pf6N2E2_3405 at 3949.788 kb on + strand, within Pf6N2E2_3405 at 3949.788 kb on + strand, within Pf6N2E2_3405 at 3949.788 kb on + strand, within Pf6N2E2_3405 at 3949.788 kb on + strand, within Pf6N2E2_3405 at 3949.789 kb on - strand, within Pf6N2E2_3405 at 3949.789 kb on - strand, within Pf6N2E2_3405 at 3949.789 kb on - strand, within Pf6N2E2_3405 at 3949.899 kb on + strand, within Pf6N2E2_3405 at 3949.899 kb on + strand, within Pf6N2E2_3405 at 3949.899 kb on + strand, within Pf6N2E2_3405 at 3949.899 kb on + strand, within Pf6N2E2_3405 at 3949.899 kb on + strand, within Pf6N2E2_3405 at 3949.900 kb on - strand, within Pf6N2E2_3405 at 3949.900 kb on - strand, within Pf6N2E2_3405 at 3949.900 kb on - strand, within Pf6N2E2_3405 at 3949.900 kb on - strand, within Pf6N2E2_3405 at 3949.950 kb on + strand, within Pf6N2E2_3405 at 3949.950 kb on + strand, within Pf6N2E2_3405 at 3949.950 kb on + strand, within Pf6N2E2_3405 at 3949.951 kb on - strand, within Pf6N2E2_3405 at 3949.966 kb on + strand, within Pf6N2E2_3405 at 3949.966 kb on + strand, within Pf6N2E2_3405
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Nickel (II) chloride 0.04 mM remove 3,946,674 - Pf6N2E2_3402 0.26 -0.2 3,946,674 - Pf6N2E2_3402 0.26 -1.3 3,946,733 + Pf6N2E2_3402 0.31 +0.9 3,946,733 + Pf6N2E2_3402 0.31 +0.9 3,946,733 + Pf6N2E2_3402 0.31 -1.1 3,946,734 - Pf6N2E2_3402 0.31 +1.2 3,946,734 - Pf6N2E2_3402 0.31 -1.0 3,946,734 - Pf6N2E2_3402 0.31 -0.8 3,946,734 - Pf6N2E2_3402 0.31 +1.8 3,946,742 - Pf6N2E2_3402 0.32 +1.0 3,946,785 + Pf6N2E2_3402 0.35 -1.4 3,946,786 - Pf6N2E2_3402 0.35 +1.2 3,946,786 - Pf6N2E2_3402 0.35 -0.5 3,946,786 - Pf6N2E2_3402 0.35 -2.6 3,946,802 - Pf6N2E2_3402 0.36 +0.5 3,947,036 + Pf6N2E2_3402 0.55 +1.0 3,947,036 + Pf6N2E2_3402 0.55 +1.2 3,947,037 - Pf6N2E2_3402 0.55 -0.5 3,947,037 - Pf6N2E2_3402 0.55 +0.9 3,947,037 - Pf6N2E2_3402 0.55 +0.4 3,947,037 - Pf6N2E2_3402 0.55 -0.2 3,947,037 - Pf6N2E2_3402 0.55 +1.0 3,947,039 - Pf6N2E2_3402 0.56 +0.4 3,947,294 + Pf6N2E2_3402 0.76 -0.6 3,947,295 - Pf6N2E2_3402 0.76 +0.1 3,947,295 - Pf6N2E2_3402 0.76 +0.7 3,947,434 + Pf6N2E2_3402 0.88 +0.9 3,947,434 + Pf6N2E2_3402 0.88 -2.0 3,947,434 + Pf6N2E2_3402 0.88 +1.3 3,947,434 + Pf6N2E2_3402 0.88 +0.6 3,947,434 + Pf6N2E2_3402 0.88 -0.8 3,947,435 - Pf6N2E2_3402 0.88 -0.6 3,947,435 - Pf6N2E2_3402 0.88 -2.0 3,947,435 - Pf6N2E2_3402 0.88 +0.7 3,947,435 - Pf6N2E2_3402 0.88 +0.6 3,947,435 - Pf6N2E2_3402 0.88 -0.8 3,947,435 - Pf6N2E2_3402 0.88 +1.2 3,947,441 - Pf6N2E2_3402 0.88 +1.3 3,947,441 - Pf6N2E2_3402 0.88 +0.6 3,947,441 - Pf6N2E2_3402 0.88 +0.0 3,947,513 + +2.5 3,947,514 - -0.0 3,947,514 - +0.2 3,947,530 - +1.5 3,947,656 - -1.1 3,947,656 - -0.2 3,947,814 + Pf6N2E2_3403 0.16 -2.4 3,947,814 + Pf6N2E2_3403 0.16 -0.7 3,947,815 - Pf6N2E2_3403 0.16 +0.6 3,947,815 - Pf6N2E2_3403 0.16 +0.2 3,947,815 - Pf6N2E2_3403 0.16 +0.0 3,947,940 + Pf6N2E2_3403 0.25 -0.4 3,947,940 + Pf6N2E2_3403 0.25 +0.4 3,947,940 + Pf6N2E2_3403 0.25 +0.4 3,947,940 + Pf6N2E2_3403 0.25 -0.9 3,947,940 + Pf6N2E2_3403 0.25 +1.6 3,947,940 + Pf6N2E2_3403 0.25 +2.2 3,947,941 - Pf6N2E2_3403 0.25 -1.0 3,947,941 - Pf6N2E2_3403 0.25 +0.3 3,947,941 - Pf6N2E2_3403 0.25 +0.1 3,948,002 + Pf6N2E2_3403 0.29 +0.4 3,948,002 + Pf6N2E2_3403 0.29 -0.2 3,948,003 - Pf6N2E2_3403 0.29 +0.3 3,948,003 - Pf6N2E2_3403 0.29 -0.1 3,948,003 - Pf6N2E2_3403 0.29 -0.1 3,948,039 + Pf6N2E2_3403 0.32 +0.9 3,948,040 - Pf6N2E2_3403 0.32 +0.4 3,948,056 - Pf6N2E2_3403 0.33 +0.3 3,948,056 - Pf6N2E2_3403 0.33 +1.3 3,948,156 + Pf6N2E2_3403 0.40 -1.1 3,948,156 + Pf6N2E2_3403 0.40 -0.8 3,948,157 - Pf6N2E2_3403 0.40 -3.1 3,948,157 - Pf6N2E2_3403 0.40 +1.5 3,948,157 - Pf6N2E2_3403 0.40 +0.3 3,948,157 - Pf6N2E2_3403 0.40 +0.4 3,948,157 - Pf6N2E2_3403 0.40 -0.5 3,948,168 - Pf6N2E2_3403 0.41 -2.9 3,948,168 - Pf6N2E2_3403 0.41 +0.2 3,948,215 - Pf6N2E2_3403 0.44 -0.3 3,948,215 - Pf6N2E2_3403 0.44 +0.6 3,948,347 - Pf6N2E2_3403 0.53 -0.9 3,948,554 + Pf6N2E2_3403 0.68 -0.5 3,948,554 + Pf6N2E2_3403 0.68 -1.3 3,948,554 + Pf6N2E2_3403 0.68 +1.4 3,948,554 + Pf6N2E2_3403 0.68 -1.6 3,948,554 + Pf6N2E2_3403 0.68 +0.4 3,948,554 + Pf6N2E2_3403 0.68 -2.0 3,948,554 + Pf6N2E2_3403 0.68 -0.0 3,948,555 - Pf6N2E2_3403 0.68 -0.1 3,948,555 - Pf6N2E2_3403 0.68 -3.1 3,948,555 - Pf6N2E2_3403 0.68 -0.7 3,948,555 - Pf6N2E2_3403 0.68 +0.5 3,948,873 + +1.2 3,948,873 + +0.9 3,948,873 + -0.8 3,948,873 + -0.1 3,948,873 + -1.6 3,948,874 - +0.6 3,948,874 - +1.2 3,948,979 + -0.7 3,948,979 + -0.5 3,948,980 - -1.5 3,948,980 - -1.8 3,949,102 + Pf6N2E2_3404 0.12 +2.2 3,949,103 - Pf6N2E2_3404 0.13 +2.2 3,949,103 - Pf6N2E2_3404 0.13 -0.4 3,949,103 - Pf6N2E2_3404 0.13 -0.2 3,949,103 - Pf6N2E2_3404 0.13 +0.9 3,949,103 - Pf6N2E2_3404 0.13 +0.2 3,949,127 + Pf6N2E2_3404 0.16 -0.6 3,949,127 + Pf6N2E2_3404 0.16 -0.0 3,949,127 + Pf6N2E2_3404 0.16 +0.6 3,949,128 - Pf6N2E2_3404 0.16 +0.2 3,949,128 - Pf6N2E2_3404 0.16 +1.5 3,949,231 + Pf6N2E2_3404 0.31 +1.8 3,949,231 + Pf6N2E2_3404 0.31 -2.7 3,949,231 + Pf6N2E2_3404 0.31 +1.4 3,949,231 + Pf6N2E2_3404 0.31 -1.2 3,949,232 - Pf6N2E2_3404 0.31 -0.8 3,949,232 - Pf6N2E2_3404 0.31 -0.2 3,949,232 - Pf6N2E2_3404 0.31 +0.6 3,949,232 - Pf6N2E2_3404 0.31 -0.4 3,949,384 + Pf6N2E2_3404 0.54 -0.4 3,949,384 + Pf6N2E2_3404 0.54 +1.2 3,949,384 + Pf6N2E2_3404 0.54 +1.2 3,949,385 - Pf6N2E2_3404 0.54 +0.2 3,949,385 - Pf6N2E2_3404 0.54 +0.1 3,949,669 + -1.3 3,949,767 + Pf6N2E2_3405 0.11 +0.1 3,949,767 + Pf6N2E2_3405 0.11 +0.3 3,949,767 + Pf6N2E2_3405 0.11 +0.1 3,949,767 + Pf6N2E2_3405 0.11 +1.0 3,949,768 - Pf6N2E2_3405 0.11 -0.1 3,949,768 - Pf6N2E2_3405 0.11 -0.3 3,949,768 - Pf6N2E2_3405 0.11 -0.8 3,949,768 - Pf6N2E2_3405 0.11 -0.3 3,949,768 - Pf6N2E2_3405 0.11 -0.3 3,949,788 + Pf6N2E2_3405 0.14 +0.0 3,949,788 + Pf6N2E2_3405 0.14 -0.1 3,949,788 + Pf6N2E2_3405 0.14 +0.8 3,949,788 + Pf6N2E2_3405 0.14 +1.7 3,949,788 + Pf6N2E2_3405 0.14 +0.4 3,949,789 - Pf6N2E2_3405 0.14 -0.7 3,949,789 - Pf6N2E2_3405 0.14 -1.4 3,949,789 - Pf6N2E2_3405 0.14 +0.5 3,949,899 + Pf6N2E2_3405 0.31 -2.1 3,949,899 + Pf6N2E2_3405 0.31 -0.7 3,949,899 + Pf6N2E2_3405 0.31 -0.4 3,949,899 + Pf6N2E2_3405 0.31 +1.2 3,949,899 + Pf6N2E2_3405 0.31 +0.2 3,949,900 - Pf6N2E2_3405 0.31 -0.2 3,949,900 - Pf6N2E2_3405 0.31 +0.6 3,949,900 - Pf6N2E2_3405 0.31 -1.1 3,949,900 - Pf6N2E2_3405 0.31 +1.5 3,949,950 + Pf6N2E2_3405 0.39 -1.7 3,949,950 + Pf6N2E2_3405 0.39 -0.6 3,949,950 + Pf6N2E2_3405 0.39 +0.8 3,949,951 - Pf6N2E2_3405 0.39 -1.4 3,949,966 + Pf6N2E2_3405 0.41 +0.3 3,949,966 + Pf6N2E2_3405 0.41 +0.6
Or see this region's nucleotide sequence