Experiment: RCH2 defined with Nickel (II) chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3311 and Pf6N2E2_3312 are separated by 10 nucleotides Pf6N2E2_3312 and Pf6N2E2_3313 are separated by 10 nucleotides Pf6N2E2_3313 and Pf6N2E2_3314 overlap by 1 nucleotides
Pf6N2E2_3311 - Dipeptide transport system permease protein DppB (TC 3.A.1.5.2), at 3,825,646 to 3,826,656
_3311
Pf6N2E2_3312 - Dipeptide transport system permease protein DppC (TC 3.A.1.5.2), at 3,826,667 to 3,827,578
_3312
Pf6N2E2_3313 - Dipeptide transport ATP-binding protein DppD (TC 3.A.1.5.2), at 3,827,589 to 3,828,557
_3313
Pf6N2E2_3314 - Dipeptide transport ATP-binding protein DppF (TC 3.A.1.5.2), at 3,828,557 to 3,829,537
_3314
Position (kb)
3826
3827
3828 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3825.822 kb on + strand, within Pf6N2E2_3311 at 3825.832 kb on + strand, within Pf6N2E2_3311 at 3825.832 kb on + strand, within Pf6N2E2_3311 at 3825.833 kb on - strand, within Pf6N2E2_3311 at 3825.842 kb on - strand, within Pf6N2E2_3311 at 3825.851 kb on - strand, within Pf6N2E2_3311 at 3826.163 kb on - strand, within Pf6N2E2_3311 at 3826.342 kb on + strand, within Pf6N2E2_3311 at 3826.342 kb on + strand, within Pf6N2E2_3311 at 3826.342 kb on + strand, within Pf6N2E2_3311 at 3826.343 kb on - strand, within Pf6N2E2_3311 at 3826.343 kb on - strand, within Pf6N2E2_3311 at 3826.343 kb on - strand, within Pf6N2E2_3311 at 3826.343 kb on - strand, within Pf6N2E2_3311 at 3826.343 kb on - strand, within Pf6N2E2_3311 at 3826.458 kb on - strand, within Pf6N2E2_3311 at 3826.544 kb on - strand, within Pf6N2E2_3311 at 3826.672 kb on + strand at 3826.672 kb on + strand at 3826.673 kb on - strand at 3827.195 kb on + strand, within Pf6N2E2_3312 at 3827.195 kb on + strand, within Pf6N2E2_3312 at 3827.195 kb on + strand, within Pf6N2E2_3312 at 3827.196 kb on - strand, within Pf6N2E2_3312 at 3827.196 kb on - strand, within Pf6N2E2_3312 at 3827.243 kb on + strand, within Pf6N2E2_3312 at 3827.244 kb on - strand, within Pf6N2E2_3312 at 3827.450 kb on + strand, within Pf6N2E2_3312 at 3827.451 kb on - strand, within Pf6N2E2_3312 at 3827.451 kb on - strand, within Pf6N2E2_3312 at 3827.600 kb on - strand at 3827.901 kb on + strand at 3828.033 kb on + strand, within Pf6N2E2_3313 at 3828.267 kb on + strand, within Pf6N2E2_3313 at 3828.267 kb on + strand, within Pf6N2E2_3313 at 3828.267 kb on + strand, within Pf6N2E2_3313 at 3828.268 kb on - strand, within Pf6N2E2_3313 at 3828.268 kb on - strand, within Pf6N2E2_3313 at 3828.331 kb on - strand, within Pf6N2E2_3313 at 3828.460 kb on - strand, within Pf6N2E2_3313 at 3828.471 kb on - strand at 3828.482 kb on - strand at 3828.532 kb on - strand at 3828.567 kb on + strand at 3828.567 kb on + strand at 3828.567 kb on + strand at 3828.568 kb on - strand at 3828.568 kb on - strand at 3828.568 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Nickel (II) chloride 0.04 mM remove 3,825,822 + Pf6N2E2_3311 0.17 -1.2 3,825,832 + Pf6N2E2_3311 0.18 +3.3 3,825,832 + Pf6N2E2_3311 0.18 +1.9 3,825,833 - Pf6N2E2_3311 0.18 +0.9 3,825,842 - Pf6N2E2_3311 0.19 +1.6 3,825,851 - Pf6N2E2_3311 0.20 +0.7 3,826,163 - Pf6N2E2_3311 0.51 +2.0 3,826,342 + Pf6N2E2_3311 0.69 -0.2 3,826,342 + Pf6N2E2_3311 0.69 +1.3 3,826,342 + Pf6N2E2_3311 0.69 +1.7 3,826,343 - Pf6N2E2_3311 0.69 +2.2 3,826,343 - Pf6N2E2_3311 0.69 +1.8 3,826,343 - Pf6N2E2_3311 0.69 +0.3 3,826,343 - Pf6N2E2_3311 0.69 +0.4 3,826,343 - Pf6N2E2_3311 0.69 +1.5 3,826,458 - Pf6N2E2_3311 0.80 +2.4 3,826,544 - Pf6N2E2_3311 0.89 +1.4 3,826,672 + +1.1 3,826,672 + -0.1 3,826,673 - +1.7 3,827,195 + Pf6N2E2_3312 0.58 +1.8 3,827,195 + Pf6N2E2_3312 0.58 +1.8 3,827,195 + Pf6N2E2_3312 0.58 +1.1 3,827,196 - Pf6N2E2_3312 0.58 -0.2 3,827,196 - Pf6N2E2_3312 0.58 +1.0 3,827,243 + Pf6N2E2_3312 0.63 +1.2 3,827,244 - Pf6N2E2_3312 0.63 +2.8 3,827,450 + Pf6N2E2_3312 0.86 +1.4 3,827,451 - Pf6N2E2_3312 0.86 +1.4 3,827,451 - Pf6N2E2_3312 0.86 +2.3 3,827,600 - +0.8 3,827,901 + +2.0 3,828,033 + Pf6N2E2_3313 0.46 +1.6 3,828,267 + Pf6N2E2_3313 0.70 +2.0 3,828,267 + Pf6N2E2_3313 0.70 +2.5 3,828,267 + Pf6N2E2_3313 0.70 +0.7 3,828,268 - Pf6N2E2_3313 0.70 +1.4 3,828,268 - Pf6N2E2_3313 0.70 +2.0 3,828,331 - Pf6N2E2_3313 0.77 -0.1 3,828,460 - Pf6N2E2_3313 0.90 +2.2 3,828,471 - +2.4 3,828,482 - +2.0 3,828,532 - +1.0 3,828,567 + +2.0 3,828,567 + -1.2 3,828,567 + +1.0 3,828,568 - -1.2 3,828,568 - +1.6 3,828,568 - +0.4
Or see this region's nucleotide sequence