Experiment: RCH2 defined with Nickel (II) chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1796 and Pf6N2E2_1797 are separated by 440 nucleotides Pf6N2E2_1797 and Pf6N2E2_1798 are separated by 295 nucleotides
Pf6N2E2_1796 - Glycine cleavage system transcriptional activator GcvA, at 2,124,977 to 2,125,777
_1796
Pf6N2E2_1797 - Transcriptional regulator, at 2,126,218 to 2,127,183
_1797
Pf6N2E2_1798 - Argininosuccinate lyase (EC 4.3.2.1), at 2,127,479 to 2,128,906
_1798
Position (kb)
2126
2127
2128 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2125.487 kb on - strand, within Pf6N2E2_1796 at 2125.895 kb on + strand at 2125.962 kb on - strand at 2126.086 kb on + strand at 2126.087 kb on - strand at 2126.087 kb on - strand at 2126.133 kb on + strand at 2126.134 kb on - strand at 2126.134 kb on - strand at 2126.141 kb on + strand at 2126.157 kb on - strand at 2126.284 kb on + strand at 2126.285 kb on - strand at 2126.382 kb on - strand, within Pf6N2E2_1797 at 2126.619 kb on - strand, within Pf6N2E2_1797 at 2126.627 kb on + strand, within Pf6N2E2_1797 at 2126.902 kb on + strand, within Pf6N2E2_1797 at 2127.191 kb on - strand at 2127.639 kb on + strand, within Pf6N2E2_1798 at 2127.752 kb on - strand, within Pf6N2E2_1798 at 2128.030 kb on - strand, within Pf6N2E2_1798 at 2128.030 kb on - strand, within Pf6N2E2_1798 at 2128.054 kb on - strand, within Pf6N2E2_1798 at 2128.054 kb on - strand, within Pf6N2E2_1798 at 2128.057 kb on + strand, within Pf6N2E2_1798 at 2128.057 kb on + strand, within Pf6N2E2_1798 at 2128.057 kb on + strand, within Pf6N2E2_1798 at 2128.057 kb on + strand, within Pf6N2E2_1798 at 2128.057 kb on + strand, within Pf6N2E2_1798 at 2128.058 kb on - strand, within Pf6N2E2_1798 at 2128.058 kb on - strand, within Pf6N2E2_1798 at 2128.058 kb on - strand, within Pf6N2E2_1798 at 2128.058 kb on - strand, within Pf6N2E2_1798
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Nickel (II) chloride 0.04 mM remove 2,125,487 - Pf6N2E2_1796 0.64 -0.3 2,125,895 + +0.8 2,125,962 - -0.5 2,126,086 + +1.1 2,126,087 - -0.6 2,126,087 - -1.0 2,126,133 + -1.5 2,126,134 - -1.5 2,126,134 - -0.1 2,126,141 + -1.0 2,126,157 - -2.5 2,126,284 + -2.3 2,126,285 - -0.5 2,126,382 - Pf6N2E2_1797 0.17 -0.1 2,126,619 - Pf6N2E2_1797 0.42 -1.0 2,126,627 + Pf6N2E2_1797 0.42 -3.0 2,126,902 + Pf6N2E2_1797 0.71 -1.0 2,127,191 - +0.1 2,127,639 + Pf6N2E2_1798 0.11 +0.5 2,127,752 - Pf6N2E2_1798 0.19 +2.7 2,128,030 - Pf6N2E2_1798 0.39 -1.5 2,128,030 - Pf6N2E2_1798 0.39 +0.9 2,128,054 - Pf6N2E2_1798 0.40 +2.0 2,128,054 - Pf6N2E2_1798 0.40 -0.9 2,128,057 + Pf6N2E2_1798 0.40 +0.5 2,128,057 + Pf6N2E2_1798 0.40 +1.7 2,128,057 + Pf6N2E2_1798 0.40 +2.0 2,128,057 + Pf6N2E2_1798 0.40 -0.6 2,128,057 + Pf6N2E2_1798 0.40 -0.5 2,128,058 - Pf6N2E2_1798 0.41 +0.5 2,128,058 - Pf6N2E2_1798 0.41 -2.0 2,128,058 - Pf6N2E2_1798 0.41 +0.7 2,128,058 - Pf6N2E2_1798 0.41 -0.6
Or see this region's nucleotide sequence