Experiment: RCH2 defined with Nickel (II) chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1028 and Pf6N2E2_1029 are separated by 126 nucleotides Pf6N2E2_1029 and Pf6N2E2_1030 are separated by 151 nucleotides
Pf6N2E2_1028 - Transcriptional regulator, LysR family, at 1,201,891 to 1,202,796
_1028
Pf6N2E2_1029 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 1,202,923 to 1,203,717
_1029
Pf6N2E2_1030 - Alkanesulfonate monooxygenase (EC 1.14.14.5), at 1,203,869 to 1,204,957
_1030
Position (kb)
1202
1203
1204 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1202.449 kb on + strand, within Pf6N2E2_1028 at 1202.449 kb on + strand, within Pf6N2E2_1028 at 1202.449 kb on + strand, within Pf6N2E2_1028 at 1202.704 kb on + strand, within Pf6N2E2_1028 at 1202.881 kb on - strand at 1202.899 kb on + strand at 1202.946 kb on + strand at 1203.058 kb on + strand, within Pf6N2E2_1029 at 1203.058 kb on + strand, within Pf6N2E2_1029 at 1203.058 kb on + strand, within Pf6N2E2_1029 at 1203.059 kb on - strand, within Pf6N2E2_1029 at 1203.059 kb on - strand, within Pf6N2E2_1029 at 1203.059 kb on - strand, within Pf6N2E2_1029 at 1203.193 kb on - strand, within Pf6N2E2_1029 at 1203.220 kb on + strand, within Pf6N2E2_1029 at 1203.446 kb on + strand, within Pf6N2E2_1029 at 1203.446 kb on + strand, within Pf6N2E2_1029 at 1203.447 kb on - strand, within Pf6N2E2_1029 at 1203.746 kb on - strand at 1203.760 kb on + strand at 1203.853 kb on - strand at 1203.945 kb on + strand at 1204.009 kb on - strand, within Pf6N2E2_1030 at 1204.009 kb on - strand, within Pf6N2E2_1030 at 1204.181 kb on + strand, within Pf6N2E2_1030 at 1204.182 kb on - strand, within Pf6N2E2_1030 at 1204.359 kb on + strand, within Pf6N2E2_1030 at 1204.380 kb on + strand, within Pf6N2E2_1030 at 1204.381 kb on - strand, within Pf6N2E2_1030 at 1204.438 kb on - strand, within Pf6N2E2_1030 at 1204.448 kb on - strand, within Pf6N2E2_1030 at 1204.448 kb on - strand, within Pf6N2E2_1030 at 1204.448 kb on - strand, within Pf6N2E2_1030 at 1204.500 kb on + strand, within Pf6N2E2_1030 at 1204.500 kb on + strand, within Pf6N2E2_1030 at 1204.500 kb on + strand, within Pf6N2E2_1030 at 1204.501 kb on - strand, within Pf6N2E2_1030 at 1204.501 kb on - strand, within Pf6N2E2_1030 at 1204.501 kb on - strand, within Pf6N2E2_1030 at 1204.501 kb on - strand, within Pf6N2E2_1030 at 1204.512 kb on + strand, within Pf6N2E2_1030 at 1204.599 kb on + strand, within Pf6N2E2_1030 at 1204.599 kb on + strand, within Pf6N2E2_1030 at 1204.599 kb on + strand, within Pf6N2E2_1030 at 1204.599 kb on + strand, within Pf6N2E2_1030 at 1204.599 kb on + strand, within Pf6N2E2_1030 at 1204.599 kb on + strand, within Pf6N2E2_1030 at 1204.600 kb on - strand, within Pf6N2E2_1030 at 1204.600 kb on - strand, within Pf6N2E2_1030 at 1204.600 kb on - strand, within Pf6N2E2_1030 at 1204.600 kb on - strand, within Pf6N2E2_1030
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Nickel (II) chloride 0.04 mM remove 1,202,449 + Pf6N2E2_1028 0.62 +2.6 1,202,449 + Pf6N2E2_1028 0.62 +0.5 1,202,449 + Pf6N2E2_1028 0.62 +0.3 1,202,704 + Pf6N2E2_1028 0.90 -1.4 1,202,881 - -0.3 1,202,899 + +0.9 1,202,946 + -0.2 1,203,058 + Pf6N2E2_1029 0.17 +1.0 1,203,058 + Pf6N2E2_1029 0.17 -1.6 1,203,058 + Pf6N2E2_1029 0.17 -0.9 1,203,059 - Pf6N2E2_1029 0.17 -1.8 1,203,059 - Pf6N2E2_1029 0.17 +0.4 1,203,059 - Pf6N2E2_1029 0.17 -1.9 1,203,193 - Pf6N2E2_1029 0.34 -2.0 1,203,220 + Pf6N2E2_1029 0.37 -1.0 1,203,446 + Pf6N2E2_1029 0.66 -1.9 1,203,446 + Pf6N2E2_1029 0.66 -1.9 1,203,447 - Pf6N2E2_1029 0.66 -1.2 1,203,746 - +2.1 1,203,760 + +1.9 1,203,853 - +1.5 1,203,945 + +1.7 1,204,009 - Pf6N2E2_1030 0.13 +0.4 1,204,009 - Pf6N2E2_1030 0.13 +0.7 1,204,181 + Pf6N2E2_1030 0.29 -0.6 1,204,182 - Pf6N2E2_1030 0.29 -1.5 1,204,359 + Pf6N2E2_1030 0.45 -1.3 1,204,380 + Pf6N2E2_1030 0.47 -1.2 1,204,381 - Pf6N2E2_1030 0.47 -1.2 1,204,438 - Pf6N2E2_1030 0.52 +0.7 1,204,448 - Pf6N2E2_1030 0.53 +0.8 1,204,448 - Pf6N2E2_1030 0.53 +0.4 1,204,448 - Pf6N2E2_1030 0.53 +0.3 1,204,500 + Pf6N2E2_1030 0.58 -3.4 1,204,500 + Pf6N2E2_1030 0.58 -0.6 1,204,500 + Pf6N2E2_1030 0.58 +1.7 1,204,501 - Pf6N2E2_1030 0.58 -0.6 1,204,501 - Pf6N2E2_1030 0.58 +0.3 1,204,501 - Pf6N2E2_1030 0.58 -3.3 1,204,501 - Pf6N2E2_1030 0.58 -0.4 1,204,512 + Pf6N2E2_1030 0.59 -1.6 1,204,599 + Pf6N2E2_1030 0.67 -1.0 1,204,599 + Pf6N2E2_1030 0.67 +2.1 1,204,599 + Pf6N2E2_1030 0.67 +1.4 1,204,599 + Pf6N2E2_1030 0.67 +0.0 1,204,599 + Pf6N2E2_1030 0.67 -0.6 1,204,599 + Pf6N2E2_1030 0.67 +0.3 1,204,600 - Pf6N2E2_1030 0.67 +0.3 1,204,600 - Pf6N2E2_1030 0.67 -0.2 1,204,600 - Pf6N2E2_1030 0.67 -0.6 1,204,600 - Pf6N2E2_1030 0.67 -0.7
Or see this region's nucleotide sequence