Experiment: MoLS4 with Putrescine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pgl and DVU2315 are separated by 281 nucleotides DVU2315 and topB are separated by 82 nucleotides
DVU2313: pgl - 6-phosphogluconolactonase (TIGR), at 2,406,614 to 2,407,363
pgl
DVU2315: DVU2315 - conserved hypothetical protein TIGR00257 (TIGR), at 2,407,645 to 2,408,373
DVU2315
DVU2316: topB - DNA topoisomerase III (TIGR), at 2,408,456 to 2,411,044
topB
Position (kb)
2407
2408
2409 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2406.716 kb on - strand, within pgl at 2406.723 kb on - strand, within pgl at 2406.759 kb on + strand, within pgl at 2406.841 kb on - strand, within pgl at 2406.856 kb on - strand, within pgl at 2406.908 kb on - strand, within pgl at 2407.004 kb on + strand, within pgl at 2407.158 kb on - strand, within pgl at 2407.164 kb on + strand, within pgl at 2407.170 kb on + strand, within pgl at 2407.170 kb on + strand, within pgl at 2407.184 kb on + strand, within pgl at 2407.401 kb on - strand at 2407.403 kb on + strand at 2407.478 kb on + strand at 2407.483 kb on + strand at 2407.500 kb on + strand at 2407.598 kb on + strand at 2407.657 kb on + strand at 2407.665 kb on - strand at 2407.673 kb on + strand at 2407.681 kb on - strand at 2407.681 kb on - strand at 2407.681 kb on - strand at 2407.681 kb on - strand at 2407.711 kb on - strand at 2407.755 kb on + strand, within DVU2315 at 2407.763 kb on - strand, within DVU2315 at 2407.770 kb on - strand, within DVU2315 at 2408.037 kb on + strand, within DVU2315 at 2408.083 kb on - strand, within DVU2315 at 2408.134 kb on - strand, within DVU2315 at 2408.172 kb on - strand, within DVU2315 at 2408.359 kb on - strand at 2408.377 kb on - strand at 2408.377 kb on - strand at 2408.441 kb on + strand at 2408.544 kb on + strand at 2408.631 kb on - strand at 2408.705 kb on + strand at 2408.710 kb on + strand at 2408.979 kb on + strand, within topB at 2409.016 kb on - strand, within topB at 2409.036 kb on + strand, within topB at 2409.036 kb on + strand, within topB at 2409.044 kb on - strand, within topB at 2409.097 kb on + strand, within topB at 2409.161 kb on + strand, within topB at 2409.161 kb on - strand, within topB at 2409.189 kb on - strand, within topB at 2409.239 kb on + strand, within topB at 2409.255 kb on + strand, within topB at 2409.255 kb on + strand, within topB at 2409.263 kb on - strand, within topB at 2409.271 kb on + strand, within topB
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with Putrescine as nutrient 10mM remove 2,406,716 - pgl DVU2313 0.14 -0.6 2,406,723 - pgl DVU2313 0.15 -1.1 2,406,759 + pgl DVU2313 0.19 -0.3 2,406,841 - pgl DVU2313 0.30 -2.1 2,406,856 - pgl DVU2313 0.32 +0.2 2,406,908 - pgl DVU2313 0.39 -1.6 2,407,004 + pgl DVU2313 0.52 -0.6 2,407,158 - pgl DVU2313 0.73 -0.4 2,407,164 + pgl DVU2313 0.73 -0.2 2,407,170 + pgl DVU2313 0.74 +0.2 2,407,170 + pgl DVU2313 0.74 -0.5 2,407,184 + pgl DVU2313 0.76 +0.3 2,407,401 - +0.2 2,407,403 + -0.0 2,407,478 + -0.1 2,407,483 + -0.2 2,407,500 + -0.3 2,407,598 + -1.6 2,407,657 + +0.4 2,407,665 - +1.0 2,407,673 + +1.0 2,407,681 - +2.8 2,407,681 - +0.2 2,407,681 - +0.0 2,407,681 - +0.4 2,407,711 - -1.5 2,407,755 + DVU2315 0.15 +0.3 2,407,763 - DVU2315 0.16 -0.0 2,407,770 - DVU2315 0.17 +0.0 2,408,037 + DVU2315 0.54 +0.7 2,408,083 - DVU2315 0.60 -0.1 2,408,134 - DVU2315 0.67 -0.2 2,408,172 - DVU2315 0.72 +0.1 2,408,359 - +0.8 2,408,377 - +0.5 2,408,377 - -0.4 2,408,441 + -0.3 2,408,544 + -0.2 2,408,631 - -0.6 2,408,705 + -0.3 2,408,710 + -0.0 2,408,979 + topB DVU2316 0.20 -0.4 2,409,016 - topB DVU2316 0.22 -0.4 2,409,036 + topB DVU2316 0.22 -0.8 2,409,036 + topB DVU2316 0.22 +1.0 2,409,044 - topB DVU2316 0.23 -0.0 2,409,097 + topB DVU2316 0.25 +0.7 2,409,161 + topB DVU2316 0.27 -0.1 2,409,161 - topB DVU2316 0.27 +0.6 2,409,189 - topB DVU2316 0.28 +0.5 2,409,239 + topB DVU2316 0.30 -0.0 2,409,255 + topB DVU2316 0.31 +0.0 2,409,255 + topB DVU2316 0.31 +1.1 2,409,263 - topB DVU2316 0.31 -0.2 2,409,271 + topB DVU2316 0.31 -0.0
Or see this region's nucleotide sequence