Experiment: nitrogen source nitrate 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS10445 and ABZR87_RS10450 are separated by 17 nucleotides ABZR87_RS10450 and ABZR87_RS10455 are separated by 91 nucleotides
ABZR87_RS10445: ABZR87_RS10445 - peptidase, at 2,213,866 to 2,214,768
_RS10445
ABZR87_RS10450: ABZR87_RS10450 - alpha/beta hydrolase, at 2,214,786 to 2,215,607
_RS10450
ABZR87_RS10455: ABZR87_RS10455 - CTP synthase, at 2,215,699 to 2,217,363
_RS10455
Position (kb)
2214
2215
2216 Strain fitness (log2 ratio)
-1
0
1
2 at 2213.824 kb on + strand at 2213.824 kb on + strand at 2213.825 kb on - strand at 2213.825 kb on - strand at 2213.825 kb on - strand at 2213.872 kb on + strand at 2213.872 kb on + strand at 2213.872 kb on + strand at 2213.872 kb on + strand at 2213.873 kb on - strand at 2213.873 kb on - strand at 2213.873 kb on - strand at 2213.873 kb on - strand at 2213.873 kb on - strand at 2213.873 kb on - strand at 2213.873 kb on - strand at 2213.873 kb on - strand at 2214.135 kb on - strand, within ABZR87_RS10445 at 2214.145 kb on + strand, within ABZR87_RS10445 at 2214.145 kb on + strand, within ABZR87_RS10445 at 2214.145 kb on + strand, within ABZR87_RS10445 at 2214.146 kb on - strand, within ABZR87_RS10445 at 2214.146 kb on - strand, within ABZR87_RS10445 at 2214.251 kb on - strand, within ABZR87_RS10445 at 2214.292 kb on + strand, within ABZR87_RS10445 at 2214.292 kb on + strand, within ABZR87_RS10445 at 2214.293 kb on - strand, within ABZR87_RS10445 at 2214.293 kb on - strand, within ABZR87_RS10445 at 2214.316 kb on + strand, within ABZR87_RS10445 at 2214.316 kb on + strand, within ABZR87_RS10445 at 2214.316 kb on + strand, within ABZR87_RS10445 at 2214.316 kb on + strand, within ABZR87_RS10445 at 2214.317 kb on - strand, within ABZR87_RS10445 at 2214.317 kb on - strand, within ABZR87_RS10445 at 2214.317 kb on - strand, within ABZR87_RS10445 at 2214.317 kb on - strand, within ABZR87_RS10445 at 2214.451 kb on + strand, within ABZR87_RS10445 at 2214.452 kb on - strand, within ABZR87_RS10445 at 2214.452 kb on - strand, within ABZR87_RS10445 at 2214.837 kb on + strand at 2214.837 kb on + strand at 2214.838 kb on - strand at 2214.838 kb on - strand at 2215.267 kb on - strand, within ABZR87_RS10450 at 2215.267 kb on - strand, within ABZR87_RS10450 at 2215.267 kb on - strand, within ABZR87_RS10450 at 2215.338 kb on + strand, within ABZR87_RS10450 at 2215.339 kb on - strand, within ABZR87_RS10450 at 2215.347 kb on + strand, within ABZR87_RS10450 at 2215.348 kb on - strand, within ABZR87_RS10450 at 2215.512 kb on + strand, within ABZR87_RS10450 at 2215.513 kb on - strand, within ABZR87_RS10450 at 2215.605 kb on + strand at 2215.668 kb on + strand at 2215.668 kb on + strand at 2215.668 kb on + strand at 2215.668 kb on + strand at 2215.669 kb on - strand at 2215.669 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 20mM remove 2,213,824 + -0.2 2,213,824 + +0.2 2,213,825 - -0.5 2,213,825 - +0.7 2,213,825 - +0.3 2,213,872 + +0.2 2,213,872 + +0.4 2,213,872 + -0.0 2,213,872 + +0.5 2,213,873 - +0.9 2,213,873 - -0.4 2,213,873 - +0.7 2,213,873 - -0.3 2,213,873 - -0.7 2,213,873 - -0.1 2,213,873 - -1.2 2,213,873 - +0.6 2,214,135 - ABZR87_RS10445 0.30 -0.3 2,214,145 + ABZR87_RS10445 0.31 +2.6 2,214,145 + ABZR87_RS10445 0.31 -0.9 2,214,145 + ABZR87_RS10445 0.31 +0.6 2,214,146 - ABZR87_RS10445 0.31 +1.7 2,214,146 - ABZR87_RS10445 0.31 +0.5 2,214,251 - ABZR87_RS10445 0.43 -0.3 2,214,292 + ABZR87_RS10445 0.47 +0.6 2,214,292 + ABZR87_RS10445 0.47 -0.4 2,214,293 - ABZR87_RS10445 0.47 -1.4 2,214,293 - ABZR87_RS10445 0.47 -0.5 2,214,316 + ABZR87_RS10445 0.50 -1.6 2,214,316 + ABZR87_RS10445 0.50 +0.7 2,214,316 + ABZR87_RS10445 0.50 +1.3 2,214,316 + ABZR87_RS10445 0.50 -1.2 2,214,317 - ABZR87_RS10445 0.50 +0.1 2,214,317 - ABZR87_RS10445 0.50 -0.8 2,214,317 - ABZR87_RS10445 0.50 +0.3 2,214,317 - ABZR87_RS10445 0.50 +0.5 2,214,451 + ABZR87_RS10445 0.65 +0.4 2,214,452 - ABZR87_RS10445 0.65 -0.1 2,214,452 - ABZR87_RS10445 0.65 -1.0 2,214,837 + -0.1 2,214,837 + +1.0 2,214,838 - -0.5 2,214,838 - +0.4 2,215,267 - ABZR87_RS10450 0.59 +1.8 2,215,267 - ABZR87_RS10450 0.59 +0.8 2,215,267 - ABZR87_RS10450 0.59 +0.0 2,215,338 + ABZR87_RS10450 0.67 +0.7 2,215,339 - ABZR87_RS10450 0.67 +0.3 2,215,347 + ABZR87_RS10450 0.68 +1.0 2,215,348 - ABZR87_RS10450 0.68 +0.7 2,215,512 + ABZR87_RS10450 0.88 +0.7 2,215,513 - ABZR87_RS10450 0.88 -1.6 2,215,605 + -0.3 2,215,668 + -0.7 2,215,668 + -0.9 2,215,668 + -0.3 2,215,668 + +0.0 2,215,669 - +0.8 2,215,669 - -0.7
Or see this region's nucleotide sequence