Experiment: Varel_Bryant_medium_Glucose with Doxycycline hyclate 0.0001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1289 and BT1290 overlap by 7 nucleotides BT1290 and BT1291 are separated by 35 nucleotides BT1291 and BT1292 are separated by 139 nucleotides
BT1289: BT1289 - putrescine transport system permease protein potI (NCBI ptt file), at 1,606,023 to 1,606,820
BT1289
BT1290: BT1290 - putrescine transport system permease protein potH (NCBI ptt file), at 1,606,814 to 1,607,590
BT1290
BT1291: BT1291 - spermidine/putrescine transport ATP-binding protein (NCBI ptt file), at 1,607,626 to 1,609,017
BT1291
BT1292: BT1292 - thiol:disulfide interchange protein (NCBI ptt file), at 1,609,157 to 1,610,257
BT1292
Position (kb)
1607
1608
1609
1610 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1606.739 kb on - strand, within BT1289 at 1606.767 kb on + strand at 1606.780 kb on - strand at 1606.816 kb on - strand at 1606.821 kb on - strand at 1606.841 kb on - strand at 1606.927 kb on + strand, within BT1290 at 1606.927 kb on + strand, within BT1290 at 1606.957 kb on + strand, within BT1290 at 1607.014 kb on + strand, within BT1290 at 1607.131 kb on - strand, within BT1290 at 1607.131 kb on - strand, within BT1290 at 1607.131 kb on - strand, within BT1290 at 1607.185 kb on + strand, within BT1290 at 1607.270 kb on - strand, within BT1290 at 1607.273 kb on + strand, within BT1290 at 1607.273 kb on + strand, within BT1290 at 1607.386 kb on + strand, within BT1290 at 1607.387 kb on - strand, within BT1290 at 1607.408 kb on - strand, within BT1290 at 1607.411 kb on + strand, within BT1290 at 1607.428 kb on + strand, within BT1290 at 1607.497 kb on + strand, within BT1290 at 1607.560 kb on - strand at 1607.594 kb on + strand at 1607.627 kb on + strand at 1607.640 kb on + strand at 1607.670 kb on + strand at 1607.756 kb on + strand at 1607.769 kb on + strand, within BT1291 at 1607.770 kb on - strand, within BT1291 at 1607.836 kb on - strand, within BT1291 at 1607.962 kb on + strand, within BT1291 at 1607.982 kb on + strand, within BT1291 at 1608.041 kb on + strand, within BT1291 at 1608.042 kb on - strand, within BT1291 at 1608.095 kb on - strand, within BT1291 at 1608.335 kb on + strand, within BT1291 at 1608.434 kb on + strand, within BT1291 at 1608.434 kb on + strand, within BT1291 at 1608.437 kb on - strand, within BT1291 at 1608.444 kb on + strand, within BT1291 at 1608.445 kb on - strand, within BT1291 at 1608.570 kb on + strand, within BT1291 at 1608.724 kb on + strand, within BT1291 at 1608.749 kb on + strand, within BT1291 at 1608.750 kb on - strand, within BT1291 at 1608.973 kb on + strand at 1609.017 kb on + strand at 1609.071 kb on + strand at 1609.077 kb on - strand at 1609.132 kb on - strand at 1609.135 kb on + strand at 1609.180 kb on + strand at 1609.232 kb on + strand at 1609.449 kb on + strand, within BT1292 at 1609.516 kb on + strand, within BT1292 at 1609.516 kb on + strand, within BT1292 at 1609.517 kb on - strand, within BT1292 at 1609.517 kb on - strand, within BT1292 at 1609.649 kb on - strand, within BT1292 at 1609.653 kb on + strand, within BT1292 at 1609.653 kb on + strand, within BT1292 at 1609.699 kb on - strand, within BT1292 at 1609.699 kb on - strand, within BT1292 at 1609.726 kb on + strand, within BT1292 at 1609.840 kb on - strand at 1609.844 kb on - strand, within BT1292 at 1609.846 kb on + strand, within BT1292 at 1609.847 kb on - strand, within BT1292 at 1609.918 kb on + strand, within BT1292 at 1609.978 kb on + strand, within BT1292 at 1609.988 kb on + strand, within BT1292 at 1609.994 kb on + strand, within BT1292
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Doxycycline hyclate 0.0001953125 mM remove 1,606,739 - BT1289 0.90 -2.3 1,606,767 + -1.7 1,606,780 - -2.4 1,606,816 - +1.5 1,606,821 - -1.1 1,606,841 - -0.7 1,606,927 + BT1290 0.15 -0.1 1,606,927 + BT1290 0.15 -2.7 1,606,957 + BT1290 0.18 -1.1 1,607,014 + BT1290 0.26 -1.4 1,607,131 - BT1290 0.41 -0.4 1,607,131 - BT1290 0.41 -0.7 1,607,131 - BT1290 0.41 -1.7 1,607,185 + BT1290 0.48 -1.1 1,607,270 - BT1290 0.59 -2.9 1,607,273 + BT1290 0.59 +3.1 1,607,273 + BT1290 0.59 -3.4 1,607,386 + BT1290 0.74 -3.6 1,607,387 - BT1290 0.74 -1.1 1,607,408 - BT1290 0.76 -1.3 1,607,411 + BT1290 0.77 -0.7 1,607,428 + BT1290 0.79 -0.5 1,607,497 + BT1290 0.88 -1.7 1,607,560 - -1.1 1,607,594 + -1.7 1,607,627 + -3.3 1,607,640 + -1.6 1,607,670 + +0.9 1,607,756 + -1.5 1,607,769 + BT1291 0.10 -0.1 1,607,770 - BT1291 0.10 +0.3 1,607,836 - BT1291 0.15 -1.7 1,607,962 + BT1291 0.24 -2.9 1,607,982 + BT1291 0.26 -2.1 1,608,041 + BT1291 0.30 -2.4 1,608,042 - BT1291 0.30 -2.1 1,608,095 - BT1291 0.34 +0.9 1,608,335 + BT1291 0.51 -1.1 1,608,434 + BT1291 0.58 -0.1 1,608,434 + BT1291 0.58 -1.4 1,608,437 - BT1291 0.58 -0.1 1,608,444 + BT1291 0.59 -2.4 1,608,445 - BT1291 0.59 -1.7 1,608,570 + BT1291 0.68 -0.9 1,608,724 + BT1291 0.79 -1.1 1,608,749 + BT1291 0.81 -1.7 1,608,750 - BT1291 0.81 -0.1 1,608,973 + -0.1 1,609,017 + -0.1 1,609,071 + +0.5 1,609,077 - -0.1 1,609,132 - -0.7 1,609,135 + -2.7 1,609,180 + +1.5 1,609,232 + +0.2 1,609,449 + BT1292 0.27 -1.9 1,609,516 + BT1292 0.33 -1.7 1,609,516 + BT1292 0.33 -0.1 1,609,517 - BT1292 0.33 +1.5 1,609,517 - BT1292 0.33 -0.5 1,609,649 - BT1292 0.45 -0.7 1,609,653 + BT1292 0.45 -0.7 1,609,653 + BT1292 0.45 +0.9 1,609,699 - BT1292 0.49 -1.1 1,609,699 - BT1292 0.49 -1.2 1,609,726 + BT1292 0.52 -0.5 1,609,840 - -0.1 1,609,844 - BT1292 0.62 -1.5 1,609,846 + BT1292 0.63 -0.1 1,609,847 - BT1292 0.63 -0.3 1,609,918 + BT1292 0.69 -1.1 1,609,978 + BT1292 0.75 -1.4 1,609,988 + BT1292 0.75 -1.7 1,609,994 + BT1292 0.76 -0.3
Or see this region's nucleotide sequence