Experiment: RCH2 defined with Zinc 0.032 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6031 and Pf6N2E2_6032 are separated by 161 nucleotides Pf6N2E2_6032 and Pf6N2E2_6033 are separated by 88 nucleotides Pf6N2E2_6033 and Pf6N2E2_6034 overlap by 4 nucleotides Pf6N2E2_6034 and Pf6N2E2_6035 are separated by 15 nucleotides
Pf6N2E2_6031 - hypothetical protein, at 6,848,181 to 6,848,705
_6031
Pf6N2E2_6032 - acetyltransferase, GNAT family, at 6,848,867 to 6,849,340
_6032
Pf6N2E2_6033 - Sterol desaturase, at 6,849,429 to 6,850,526
_6033
Pf6N2E2_6034 - FIG00965471: hypothetical protein, at 6,850,523 to 6,851,338
_6034
Pf6N2E2_6035 - alpha/beta hydrolase fold, at 6,851,354 to 6,852,034
_6035
Position (kb)
6849
6850
6851 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6848.551 kb on + strand, within Pf6N2E2_6031 at 6848.551 kb on + strand, within Pf6N2E2_6031 at 6848.741 kb on + strand at 6848.741 kb on + strand at 6848.742 kb on - strand at 6848.742 kb on - strand at 6848.820 kb on + strand at 6848.820 kb on + strand at 6848.821 kb on - strand at 6848.821 kb on - strand at 6848.824 kb on + strand at 6848.824 kb on + strand at 6848.824 kb on + strand at 6848.824 kb on + strand at 6848.824 kb on + strand at 6848.824 kb on + strand at 6848.824 kb on + strand at 6848.824 kb on + strand at 6848.824 kb on + strand at 6848.824 kb on + strand at 6848.825 kb on - strand at 6848.825 kb on - strand at 6848.825 kb on - strand at 6848.825 kb on - strand at 6848.825 kb on - strand at 6848.825 kb on - strand at 6848.825 kb on - strand at 6848.825 kb on - strand at 6848.825 kb on - strand at 6848.825 kb on - strand at 6848.825 kb on - strand at 6848.847 kb on + strand at 6848.847 kb on + strand at 6848.847 kb on + strand at 6848.893 kb on + strand at 6848.893 kb on + strand at 6848.926 kb on + strand, within Pf6N2E2_6032 at 6848.926 kb on + strand, within Pf6N2E2_6032 at 6848.926 kb on + strand, within Pf6N2E2_6032 at 6848.927 kb on - strand, within Pf6N2E2_6032 at 6848.927 kb on - strand, within Pf6N2E2_6032 at 6848.927 kb on - strand, within Pf6N2E2_6032 at 6849.037 kb on + strand, within Pf6N2E2_6032 at 6849.038 kb on - strand, within Pf6N2E2_6032 at 6849.038 kb on - strand, within Pf6N2E2_6032 at 6849.187 kb on - strand, within Pf6N2E2_6032 at 6849.278 kb on - strand, within Pf6N2E2_6032 at 6849.278 kb on - strand, within Pf6N2E2_6032 at 6849.283 kb on + strand, within Pf6N2E2_6032 at 6849.283 kb on + strand, within Pf6N2E2_6032 at 6849.313 kb on + strand at 6849.313 kb on + strand at 6849.313 kb on + strand at 6849.394 kb on + strand at 6849.450 kb on + strand at 6849.450 kb on + strand at 6849.451 kb on - strand at 6849.451 kb on - strand at 6849.451 kb on - strand at 6849.451 kb on - strand at 6849.552 kb on + strand, within Pf6N2E2_6033 at 6849.553 kb on - strand, within Pf6N2E2_6033 at 6849.678 kb on + strand, within Pf6N2E2_6033 at 6849.678 kb on + strand, within Pf6N2E2_6033 at 6849.678 kb on + strand, within Pf6N2E2_6033 at 6849.678 kb on + strand, within Pf6N2E2_6033 at 6849.681 kb on + strand, within Pf6N2E2_6033 at 6849.681 kb on + strand, within Pf6N2E2_6033 at 6849.744 kb on + strand, within Pf6N2E2_6033 at 6849.744 kb on + strand, within Pf6N2E2_6033 at 6849.744 kb on + strand, within Pf6N2E2_6033 at 6849.744 kb on + strand, within Pf6N2E2_6033 at 6849.745 kb on - strand, within Pf6N2E2_6033 at 6849.745 kb on - strand, within Pf6N2E2_6033 at 6849.745 kb on - strand, within Pf6N2E2_6033 at 6849.766 kb on - strand, within Pf6N2E2_6033 at 6849.838 kb on - strand, within Pf6N2E2_6033 at 6849.877 kb on - strand, within Pf6N2E2_6033 at 6849.949 kb on + strand, within Pf6N2E2_6033 at 6850.047 kb on + strand, within Pf6N2E2_6033 at 6850.048 kb on - strand, within Pf6N2E2_6033 at 6850.048 kb on - strand, within Pf6N2E2_6033 at 6850.048 kb on - strand, within Pf6N2E2_6033 at 6850.095 kb on + strand, within Pf6N2E2_6033 at 6850.095 kb on + strand, within Pf6N2E2_6033 at 6850.096 kb on - strand, within Pf6N2E2_6033 at 6850.290 kb on + strand, within Pf6N2E2_6033 at 6850.290 kb on + strand, within Pf6N2E2_6033 at 6850.291 kb on - strand, within Pf6N2E2_6033 at 6850.291 kb on - strand, within Pf6N2E2_6033 at 6850.291 kb on - strand, within Pf6N2E2_6033 at 6850.365 kb on + strand, within Pf6N2E2_6033 at 6850.365 kb on + strand, within Pf6N2E2_6033 at 6850.365 kb on + strand, within Pf6N2E2_6033 at 6850.365 kb on + strand, within Pf6N2E2_6033 at 6850.366 kb on - strand, within Pf6N2E2_6033 at 6850.366 kb on - strand, within Pf6N2E2_6033 at 6850.366 kb on - strand, within Pf6N2E2_6033 at 6850.366 kb on - strand, within Pf6N2E2_6033 at 6850.366 kb on - strand, within Pf6N2E2_6033 at 6850.366 kb on - strand, within Pf6N2E2_6033 at 6850.366 kb on - strand, within Pf6N2E2_6033 at 6850.366 kb on - strand, within Pf6N2E2_6033 at 6850.367 kb on + strand, within Pf6N2E2_6033 at 6850.408 kb on - strand, within Pf6N2E2_6033 at 6850.426 kb on - strand at 6850.718 kb on + strand, within Pf6N2E2_6034 at 6850.718 kb on + strand, within Pf6N2E2_6034 at 6850.843 kb on + strand, within Pf6N2E2_6034 at 6850.897 kb on + strand, within Pf6N2E2_6034 at 6850.897 kb on + strand, within Pf6N2E2_6034 at 6850.914 kb on - strand, within Pf6N2E2_6034 at 6850.914 kb on - strand, within Pf6N2E2_6034 at 6850.914 kb on - strand, within Pf6N2E2_6034 at 6851.209 kb on - strand, within Pf6N2E2_6034 at 6851.234 kb on + strand, within Pf6N2E2_6034 at 6851.235 kb on - strand, within Pf6N2E2_6034 at 6851.235 kb on - strand, within Pf6N2E2_6034 at 6851.305 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Zinc 0.032 mM remove 6,848,551 + Pf6N2E2_6031 0.70 +0.2 6,848,551 + Pf6N2E2_6031 0.70 -0.1 6,848,741 + -0.3 6,848,741 + -0.8 6,848,742 - +0.5 6,848,742 - -0.4 6,848,820 + -0.8 6,848,820 + -1.0 6,848,821 - +0.5 6,848,821 - -1.0 6,848,824 + +0.2 6,848,824 + -0.7 6,848,824 + +0.4 6,848,824 + +0.6 6,848,824 + +0.9 6,848,824 + +0.6 6,848,824 + -0.2 6,848,824 + -0.3 6,848,824 + -0.9 6,848,824 + -0.5 6,848,825 - -0.1 6,848,825 - +0.1 6,848,825 - +0.7 6,848,825 - +0.1 6,848,825 - +0.3 6,848,825 - +1.7 6,848,825 - +0.4 6,848,825 - -1.7 6,848,825 - +0.8 6,848,825 - -0.6 6,848,825 - -0.1 6,848,847 + -0.4 6,848,847 + -2.6 6,848,847 + +0.4 6,848,893 + +0.2 6,848,893 + -0.2 6,848,926 + Pf6N2E2_6032 0.12 -0.1 6,848,926 + Pf6N2E2_6032 0.12 -0.7 6,848,926 + Pf6N2E2_6032 0.12 +0.8 6,848,927 - Pf6N2E2_6032 0.13 -0.5 6,848,927 - Pf6N2E2_6032 0.13 +0.2 6,848,927 - Pf6N2E2_6032 0.13 +0.2 6,849,037 + Pf6N2E2_6032 0.36 -0.6 6,849,038 - Pf6N2E2_6032 0.36 -1.1 6,849,038 - Pf6N2E2_6032 0.36 +1.1 6,849,187 - Pf6N2E2_6032 0.68 +0.8 6,849,278 - Pf6N2E2_6032 0.87 -0.1 6,849,278 - Pf6N2E2_6032 0.87 -1.0 6,849,283 + Pf6N2E2_6032 0.88 -0.3 6,849,283 + Pf6N2E2_6032 0.88 -1.3 6,849,313 + -0.7 6,849,313 + +1.2 6,849,313 + -2.0 6,849,394 + -0.4 6,849,450 + -0.3 6,849,450 + +0.5 6,849,451 - -0.5 6,849,451 - +1.5 6,849,451 - +0.5 6,849,451 - +1.0 6,849,552 + Pf6N2E2_6033 0.11 -1.2 6,849,553 - Pf6N2E2_6033 0.11 -0.9 6,849,678 + Pf6N2E2_6033 0.23 -0.8 6,849,678 + Pf6N2E2_6033 0.23 +1.1 6,849,678 + Pf6N2E2_6033 0.23 -0.4 6,849,678 + Pf6N2E2_6033 0.23 -0.5 6,849,681 + Pf6N2E2_6033 0.23 -0.1 6,849,681 + Pf6N2E2_6033 0.23 -0.6 6,849,744 + Pf6N2E2_6033 0.29 -0.5 6,849,744 + Pf6N2E2_6033 0.29 -1.5 6,849,744 + Pf6N2E2_6033 0.29 -0.1 6,849,744 + Pf6N2E2_6033 0.29 -0.5 6,849,745 - Pf6N2E2_6033 0.29 +0.1 6,849,745 - Pf6N2E2_6033 0.29 -0.9 6,849,745 - Pf6N2E2_6033 0.29 -1.5 6,849,766 - Pf6N2E2_6033 0.31 +0.4 6,849,838 - Pf6N2E2_6033 0.37 -1.5 6,849,877 - Pf6N2E2_6033 0.41 -1.1 6,849,949 + Pf6N2E2_6033 0.47 -0.9 6,850,047 + Pf6N2E2_6033 0.56 -0.1 6,850,048 - Pf6N2E2_6033 0.56 -2.5 6,850,048 - Pf6N2E2_6033 0.56 +0.0 6,850,048 - Pf6N2E2_6033 0.56 -0.0 6,850,095 + Pf6N2E2_6033 0.61 -0.9 6,850,095 + Pf6N2E2_6033 0.61 -0.2 6,850,096 - Pf6N2E2_6033 0.61 +1.2 6,850,290 + Pf6N2E2_6033 0.78 -1.4 6,850,290 + Pf6N2E2_6033 0.78 -1.3 6,850,291 - Pf6N2E2_6033 0.79 +0.9 6,850,291 - Pf6N2E2_6033 0.79 +0.2 6,850,291 - Pf6N2E2_6033 0.79 +0.5 6,850,365 + Pf6N2E2_6033 0.85 +2.2 6,850,365 + Pf6N2E2_6033 0.85 +0.6 6,850,365 + Pf6N2E2_6033 0.85 -0.9 6,850,365 + Pf6N2E2_6033 0.85 +0.1 6,850,366 - Pf6N2E2_6033 0.85 -0.3 6,850,366 - Pf6N2E2_6033 0.85 -0.9 6,850,366 - Pf6N2E2_6033 0.85 -2.2 6,850,366 - Pf6N2E2_6033 0.85 +0.7 6,850,366 - Pf6N2E2_6033 0.85 +0.0 6,850,366 - Pf6N2E2_6033 0.85 +0.3 6,850,366 - Pf6N2E2_6033 0.85 -1.2 6,850,366 - Pf6N2E2_6033 0.85 +1.3 6,850,367 + Pf6N2E2_6033 0.85 -0.5 6,850,408 - Pf6N2E2_6033 0.89 -2.0 6,850,426 - -0.7 6,850,718 + Pf6N2E2_6034 0.24 +0.7 6,850,718 + Pf6N2E2_6034 0.24 -0.7 6,850,843 + Pf6N2E2_6034 0.39 +1.2 6,850,897 + Pf6N2E2_6034 0.46 +0.3 6,850,897 + Pf6N2E2_6034 0.46 -0.4 6,850,914 - Pf6N2E2_6034 0.48 -0.1 6,850,914 - Pf6N2E2_6034 0.48 +0.1 6,850,914 - Pf6N2E2_6034 0.48 +0.3 6,851,209 - Pf6N2E2_6034 0.84 +0.6 6,851,234 + Pf6N2E2_6034 0.87 +0.1 6,851,235 - Pf6N2E2_6034 0.87 +1.0 6,851,235 - Pf6N2E2_6034 0.87 +0.8 6,851,305 + -1.6
Or see this region's nucleotide sequence