Experiment: RCH2 defined with Zinc 0.032 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_446 and Pf6N2E2_447 are separated by 174 nucleotides Pf6N2E2_447 and Pf6N2E2_448 are separated by 124 nucleotides Pf6N2E2_448 and Pf6N2E2_449 are separated by 87 nucleotides Pf6N2E2_449 and Pf6N2E2_450 are separated by 194 nucleotides
Pf6N2E2_446 - Glutathione reductase (EC 1.8.1.7), at 508,167 to 509,525
_446
Pf6N2E2_447 - Alkyl hydroperoxide reductase protein C (EC 1.6.4.-), at 509,700 to 510,263
_447
Pf6N2E2_448 - Alkyl hydroperoxide reductase protein F (EC 1.6.4.-), at 510,388 to 511,950
_448
Pf6N2E2_449 - Lactoylglutathione lyase (EC 4.4.1.5), at 512,038 to 512,559
_449
Pf6N2E2_450 - FIG00953950: hypothetical protein, at 512,754 to 513,281
_450
Position (kb)
510
511
512 Strain fitness (log2 ratio)
-2
-1
0
1 at 509.507 kb on + strand at 509.540 kb on + strand at 509.540 kb on + strand at 509.540 kb on + strand at 509.540 kb on + strand at 509.540 kb on + strand at 509.540 kb on + strand at 509.540 kb on + strand at 509.540 kb on + strand at 509.541 kb on - strand at 509.541 kb on - strand at 509.541 kb on - strand at 509.541 kb on - strand at 509.580 kb on - strand at 509.583 kb on + strand at 509.584 kb on - strand at 509.656 kb on + strand at 509.656 kb on + strand at 509.656 kb on + strand at 509.706 kb on - strand at 509.706 kb on - strand at 509.706 kb on - strand at 509.724 kb on - strand at 509.814 kb on + strand, within Pf6N2E2_447 at 509.814 kb on + strand, within Pf6N2E2_447 at 509.904 kb on + strand, within Pf6N2E2_447 at 509.905 kb on - strand, within Pf6N2E2_447 at 509.905 kb on - strand, within Pf6N2E2_447 at 509.979 kb on + strand, within Pf6N2E2_447 at 509.980 kb on - strand, within Pf6N2E2_447 at 509.980 kb on - strand, within Pf6N2E2_447 at 509.980 kb on - strand, within Pf6N2E2_447 at 510.169 kb on - strand, within Pf6N2E2_447 at 510.169 kb on - strand, within Pf6N2E2_447 at 510.169 kb on - strand, within Pf6N2E2_447 at 510.261 kb on + strand at 510.261 kb on + strand at 510.262 kb on - strand at 510.262 kb on - strand at 510.262 kb on - strand at 510.262 kb on - strand at 510.262 kb on - strand at 510.262 kb on - strand at 510.262 kb on - strand at 510.298 kb on + strand at 510.298 kb on + strand at 510.298 kb on + strand at 510.298 kb on + strand at 510.299 kb on - strand at 510.299 kb on - strand at 510.299 kb on - strand at 510.299 kb on - strand at 510.299 kb on - strand at 510.299 kb on - strand at 510.299 kb on - strand at 510.387 kb on + strand at 510.388 kb on - strand at 510.388 kb on - strand at 510.405 kb on + strand at 510.405 kb on + strand at 510.424 kb on + strand at 510.504 kb on - strand at 510.538 kb on - strand at 510.538 kb on - strand at 510.538 kb on - strand at 510.538 kb on - strand at 510.754 kb on + strand, within Pf6N2E2_448 at 510.754 kb on + strand, within Pf6N2E2_448 at 510.755 kb on - strand, within Pf6N2E2_448 at 510.755 kb on - strand, within Pf6N2E2_448 at 510.755 kb on - strand, within Pf6N2E2_448 at 510.907 kb on + strand, within Pf6N2E2_448 at 510.908 kb on - strand, within Pf6N2E2_448 at 511.072 kb on + strand, within Pf6N2E2_448 at 511.072 kb on + strand, within Pf6N2E2_448 at 511.072 kb on + strand, within Pf6N2E2_448 at 511.073 kb on - strand, within Pf6N2E2_448 at 511.083 kb on + strand, within Pf6N2E2_448 at 511.222 kb on + strand, within Pf6N2E2_448 at 511.391 kb on - strand, within Pf6N2E2_448 at 511.411 kb on + strand, within Pf6N2E2_448 at 511.473 kb on + strand, within Pf6N2E2_448 at 511.473 kb on + strand, within Pf6N2E2_448 at 511.608 kb on + strand, within Pf6N2E2_448 at 511.609 kb on - strand, within Pf6N2E2_448 at 511.861 kb on + strand at 511.861 kb on + strand at 511.862 kb on - strand at 511.948 kb on + strand at 512.039 kb on + strand at 512.040 kb on - strand at 512.072 kb on + strand at 512.073 kb on - strand at 512.237 kb on + strand, within Pf6N2E2_449 at 512.370 kb on - strand, within Pf6N2E2_449 at 512.370 kb on - strand, within Pf6N2E2_449 at 512.370 kb on - strand, within Pf6N2E2_449 at 512.435 kb on + strand, within Pf6N2E2_449 at 512.573 kb on - strand at 512.574 kb on + strand at 512.574 kb on + strand at 512.574 kb on + strand at 512.574 kb on + strand at 512.574 kb on + strand at 512.574 kb on + strand at 512.574 kb on + strand at 512.574 kb on + strand at 512.574 kb on + strand at 512.575 kb on - strand at 512.575 kb on - strand at 512.575 kb on - strand at 512.575 kb on - strand at 512.575 kb on - strand at 512.658 kb on + strand at 512.659 kb on - strand at 512.659 kb on - strand at 512.659 kb on - strand at 512.659 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Zinc 0.032 mM remove 509,507 + +0.0 509,540 + -1.1 509,540 + -0.6 509,540 + -0.9 509,540 + +0.5 509,540 + -1.1 509,540 + +0.4 509,540 + -0.1 509,540 + +1.0 509,541 - +1.1 509,541 - -1.1 509,541 - +1.5 509,541 - -0.3 509,580 - +0.4 509,583 + +0.5 509,584 - +0.6 509,656 + -1.4 509,656 + -0.9 509,656 + -0.5 509,706 - +0.5 509,706 - -0.8 509,706 - +0.2 509,724 - -0.0 509,814 + Pf6N2E2_447 0.20 -1.2 509,814 + Pf6N2E2_447 0.20 +0.4 509,904 + Pf6N2E2_447 0.36 -2.3 509,905 - Pf6N2E2_447 0.36 +0.4 509,905 - Pf6N2E2_447 0.36 +0.4 509,979 + Pf6N2E2_447 0.49 +1.4 509,980 - Pf6N2E2_447 0.50 -1.6 509,980 - Pf6N2E2_447 0.50 -1.2 509,980 - Pf6N2E2_447 0.50 -0.6 510,169 - Pf6N2E2_447 0.83 +0.2 510,169 - Pf6N2E2_447 0.83 -0.2 510,169 - Pf6N2E2_447 0.83 -0.2 510,261 + -0.3 510,261 + +1.4 510,262 - +1.6 510,262 - -1.3 510,262 - -1.3 510,262 - -1.1 510,262 - +0.4 510,262 - -0.2 510,262 - +1.0 510,298 + -0.4 510,298 + -0.8 510,298 + -0.3 510,298 + -0.4 510,299 - -0.3 510,299 - -0.1 510,299 - -0.3 510,299 - -0.7 510,299 - -0.4 510,299 - -0.3 510,299 - +1.6 510,387 + -0.2 510,388 - +0.1 510,388 - -2.0 510,405 + +0.2 510,405 + +1.1 510,424 + +0.4 510,504 - -0.5 510,538 - -0.7 510,538 - +0.5 510,538 - +0.1 510,538 - -1.2 510,754 + Pf6N2E2_448 0.23 +0.1 510,754 + Pf6N2E2_448 0.23 +0.1 510,755 - Pf6N2E2_448 0.23 +1.0 510,755 - Pf6N2E2_448 0.23 -0.5 510,755 - Pf6N2E2_448 0.23 +0.6 510,907 + Pf6N2E2_448 0.33 +0.5 510,908 - Pf6N2E2_448 0.33 +0.1 511,072 + Pf6N2E2_448 0.44 +0.4 511,072 + Pf6N2E2_448 0.44 -0.4 511,072 + Pf6N2E2_448 0.44 -0.8 511,073 - Pf6N2E2_448 0.44 -1.8 511,083 + Pf6N2E2_448 0.44 -0.2 511,222 + Pf6N2E2_448 0.53 -0.3 511,391 - Pf6N2E2_448 0.64 -0.6 511,411 + Pf6N2E2_448 0.65 -0.1 511,473 + Pf6N2E2_448 0.69 +0.3 511,473 + Pf6N2E2_448 0.69 +0.1 511,608 + Pf6N2E2_448 0.78 +0.6 511,609 - Pf6N2E2_448 0.78 +0.6 511,861 + -1.4 511,861 + +0.3 511,862 - +0.2 511,948 + -0.3 512,039 + +0.9 512,040 - -0.1 512,072 + +0.0 512,073 - -0.3 512,237 + Pf6N2E2_449 0.38 +0.8 512,370 - Pf6N2E2_449 0.64 -0.4 512,370 - Pf6N2E2_449 0.64 -0.4 512,370 - Pf6N2E2_449 0.64 -0.1 512,435 + Pf6N2E2_449 0.76 +0.8 512,573 - -0.3 512,574 + -1.4 512,574 + +0.0 512,574 + -1.4 512,574 + -0.1 512,574 + +1.2 512,574 + +0.2 512,574 + +0.7 512,574 + +0.9 512,574 + -0.8 512,575 - +0.6 512,575 - -0.1 512,575 - -1.2 512,575 - +0.8 512,575 - -1.3 512,658 + +0.5 512,659 - +0.6 512,659 - -2.1 512,659 - -0.1 512,659 - -0.2
Or see this region's nucleotide sequence