Experiment: RCH2 defined with Zinc 0.032 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_208 and Pf6N2E2_209 are separated by 146 nucleotides Pf6N2E2_209 and Pf6N2E2_210 are separated by 181 nucleotides
Pf6N2E2_208 - Glycosyl transferase, group 2 family protein, at 260,782 to 263,229
_208
Pf6N2E2_209 - O-antigen export system permease protein RfbD, at 263,376 to 264,194
_209
Pf6N2E2_210 - Teichoic acid export ATP-binding protein TagH (EC 3.6.3.40), at 264,376 to 267,099
_210
Position (kb) 263
264
265 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 262.427 kb on + strand, within Pf6N2E2_208 at 262.427 kb on + strand, within Pf6N2E2_208 at 262.427 kb on + strand, within Pf6N2E2_208 at 262.428 kb on - strand, within Pf6N2E2_208 at 262.428 kb on - strand, within Pf6N2E2_208 at 262.428 kb on - strand, within Pf6N2E2_208 at 262.540 kb on - strand, within Pf6N2E2_208 at 262.541 kb on + strand, within Pf6N2E2_208 at 262.542 kb on - strand, within Pf6N2E2_208 at 262.542 kb on - strand, within Pf6N2E2_208 at 262.830 kb on + strand, within Pf6N2E2_208 at 262.832 kb on + strand, within Pf6N2E2_208 at 262.990 kb on + strand at 262.991 kb on - strand at 263.255 kb on - strand at 263.266 kb on + strand at 263.266 kb on + strand at 263.267 kb on - strand at 263.379 kb on + strand at 263.380 kb on - strand at 263.380 kb on - strand at 263.380 kb on - strand at 263.380 kb on - strand at 263.588 kb on - strand, within Pf6N2E2_209 at 263.624 kb on + strand, within Pf6N2E2_209 at 263.625 kb on - strand, within Pf6N2E2_209 at 263.656 kb on + strand, within Pf6N2E2_209 at 263.657 kb on - strand, within Pf6N2E2_209 at 263.664 kb on + strand, within Pf6N2E2_209 at 263.770 kb on + strand, within Pf6N2E2_209 at 263.770 kb on + strand, within Pf6N2E2_209 at 263.770 kb on + strand, within Pf6N2E2_209 at 263.771 kb on - strand at 263.872 kb on + strand, within Pf6N2E2_209 at 263.872 kb on + strand, within Pf6N2E2_209 at 263.873 kb on - strand, within Pf6N2E2_209 at 264.032 kb on - strand, within Pf6N2E2_209 at 264.069 kb on + strand, within Pf6N2E2_209 at 264.069 kb on + strand, within Pf6N2E2_209 at 264.069 kb on + strand, within Pf6N2E2_209 at 264.069 kb on + strand, within Pf6N2E2_209 at 264.069 kb on + strand, within Pf6N2E2_209 at 264.070 kb on - strand, within Pf6N2E2_209 at 264.070 kb on - strand, within Pf6N2E2_209 at 264.070 kb on - strand, within Pf6N2E2_209 at 264.070 kb on - strand, within Pf6N2E2_209 at 264.070 kb on - strand, within Pf6N2E2_209 at 264.070 kb on - strand, within Pf6N2E2_209 at 264.094 kb on + strand, within Pf6N2E2_209 at 264.127 kb on + strand at 264.127 kb on + strand at 264.127 kb on + strand at 264.128 kb on - strand at 264.128 kb on - strand at 264.250 kb on + strand at 264.261 kb on + strand at 264.261 kb on + strand at 264.262 kb on - strand at 264.296 kb on + strand at 264.296 kb on + strand at 264.297 kb on - strand at 264.344 kb on + strand at 264.430 kb on + strand at 264.430 kb on + strand at 264.430 kb on + strand at 264.431 kb on - strand at 264.431 kb on - strand at 264.709 kb on + strand, within Pf6N2E2_210 at 264.709 kb on + strand, within Pf6N2E2_210 at 264.709 kb on + strand, within Pf6N2E2_210 at 264.820 kb on + strand, within Pf6N2E2_210 at 265.033 kb on + strand, within Pf6N2E2_210 at 265.033 kb on + strand, within Pf6N2E2_210 at 265.034 kb on - strand, within Pf6N2E2_210
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Zinc 0.032 mM remove 262,427 + Pf6N2E2_208 0.67 -0.6 262,427 + Pf6N2E2_208 0.67 +1.2 262,427 + Pf6N2E2_208 0.67 -0.4 262,428 - Pf6N2E2_208 0.67 +1.5 262,428 - Pf6N2E2_208 0.67 +3.0 262,428 - Pf6N2E2_208 0.67 -1.7 262,540 - Pf6N2E2_208 0.72 -0.7 262,541 + Pf6N2E2_208 0.72 +0.7 262,542 - Pf6N2E2_208 0.72 -0.3 262,542 - Pf6N2E2_208 0.72 +2.2 262,830 + Pf6N2E2_208 0.84 -0.7 262,832 + Pf6N2E2_208 0.84 -0.1 262,990 + -0.2 262,991 - +0.7 263,255 - -0.6 263,266 + -1.0 263,266 + +0.7 263,267 - -0.6 263,379 + +0.5 263,380 - -0.1 263,380 - +0.6 263,380 - -1.6 263,380 - +0.6 263,588 - Pf6N2E2_209 0.26 -1.1 263,624 + Pf6N2E2_209 0.30 -0.8 263,625 - Pf6N2E2_209 0.30 +0.5 263,656 + Pf6N2E2_209 0.34 -0.2 263,657 - Pf6N2E2_209 0.34 -0.8 263,664 + Pf6N2E2_209 0.35 +0.3 263,770 + Pf6N2E2_209 0.48 -0.5 263,770 + Pf6N2E2_209 0.48 -0.5 263,770 + Pf6N2E2_209 0.48 +0.4 263,771 - +1.4 263,872 + Pf6N2E2_209 0.61 +0.5 263,872 + Pf6N2E2_209 0.61 -0.7 263,873 - Pf6N2E2_209 0.61 -0.2 264,032 - Pf6N2E2_209 0.80 -0.0 264,069 + Pf6N2E2_209 0.85 -1.0 264,069 + Pf6N2E2_209 0.85 -0.1 264,069 + Pf6N2E2_209 0.85 -0.7 264,069 + Pf6N2E2_209 0.85 -0.8 264,069 + Pf6N2E2_209 0.85 -0.7 264,070 - Pf6N2E2_209 0.85 -0.6 264,070 - Pf6N2E2_209 0.85 +1.4 264,070 - Pf6N2E2_209 0.85 -1.5 264,070 - Pf6N2E2_209 0.85 -0.7 264,070 - Pf6N2E2_209 0.85 +1.0 264,070 - Pf6N2E2_209 0.85 -0.2 264,094 + Pf6N2E2_209 0.88 -1.0 264,127 + +0.9 264,127 + +1.8 264,127 + -0.2 264,128 - +1.0 264,128 - +0.0 264,250 + -1.3 264,261 + -1.6 264,261 + -0.4 264,262 - +0.5 264,296 + -0.1 264,296 + -0.6 264,297 - -0.2 264,344 + -0.2 264,430 + -0.2 264,430 + -0.2 264,430 + -3.0 264,431 - -0.7 264,431 - -0.6 264,709 + Pf6N2E2_210 0.12 +0.1 264,709 + Pf6N2E2_210 0.12 +1.4 264,709 + Pf6N2E2_210 0.12 -0.3 264,820 + Pf6N2E2_210 0.16 +1.4 265,033 + Pf6N2E2_210 0.24 -0.9 265,033 + Pf6N2E2_210 0.24 -1.9 265,034 - Pf6N2E2_210 0.24 -0.4
Or see this region's nucleotide sequence